2011 International Workshop on Biophotonics最新文献_第3页

In-vivo non-linear imaging of collagen before and after laser micro-ablative fractional resurfacing treatment 激光微烧蚀分次表面置换治疗前后胶原蛋白的体内非线性成像

2011 International Workshop on Biophotonics Pub Date : 2011-06-08 DOI: 10.1109/IWBP.2011.5954830

R. Cicchi, D. Kapsokalyvas, F. Pavone, M. Troiano, P. Campolmi, C. Morini, T. Lotti

{"title":"In-vivo non-linear imaging of collagen before and after laser micro-ablative fractional resurfacing treatment","authors":"R. Cicchi, D. Kapsokalyvas, F. Pavone, M. Troiano, P. Campolmi, C. Morini, T. Lotti","doi":"10.1109/IWBP.2011.5954830","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954830","url":null,"abstract":"Second-harmonic generation and two-photon excited fluorescence microscopy were used in combination in the same optical system for in-vivo imaging. This work aimed at detecting collagen remodeling and reorganization in living subjects following laser micro-ablative fractional rejuvenation treatment. Treated regions in the forearm of volunteers covering a wide age range were imaged with two-photon microscopy before and after the treatment. A strong age-dependence of the treatment effectiveness was found, demonstrating a negligible effect in very young subjects (age < 30 years) face to a significant synthesis of new collagen in the most aged subjects ( age > 60 years). The amount of newly synthesized collagen as well as its organization were evaluated by means of both visual examination and an image analysis methods, based on second-harmonic to autofluorescence ageing index of dermis (SAAID) scoring. The obtained results demonstrate the performance of laser fractional micro ablative rejuvenation without the need of an invasive biopsy as well as the wide applicability range of applications for multiphoton microscopy in clinical dermatology.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"78 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128392408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Principal component analysis of data from laser scanning flow cytometry 激光扫描流式细胞术数据的主成分分析

2011 International Workshop on Biophotonics Pub Date : 2011-06-08 DOI: 10.1109/IWBP.2011.5954825

A. Palucci, V. Spizzichino, F. Angelini

引用次数: 0

Blue and red upconverted emission from fluoride crystal nanoparticles 氟化物晶体纳米粒子的蓝色和红色上转换辐射

2011 International Workshop on Biophotonics Pub Date : 2011-06-08 DOI: 10.1109/IWBP.2011.5954826

A. Toncelli, B. Ahmadi

引用次数: 1

Detection of biomolecules using light scattering 光散射法检测生物分子

2011 International Workshop on Biophotonics Pub Date : 2011-06-08 DOI: 10.1109/IWBP.2011.5954839

C. Morasso, F. Gramatica, T. Bellini, D. Prosperi

引用次数: 0

Combined optical trapping and nanometer-precision localization for the single-molecule study of DNA-binding proteins 结合光学捕获和纳米精确定位的dna结合蛋白单分子研究

2011 International Workshop on Biophotonics Pub Date : 2011-06-08 DOI: 10.1109/IWBP.2011.5954832

C. Monico, G. Belcastro, M. Capitanio, F. Vanzi, F. Pavone

{"title":"Combined optical trapping and nanometer-precision localization for the single-molecule study of DNA-binding proteins","authors":"C. Monico, G. Belcastro, M. Capitanio, F. Vanzi, F. Pavone","doi":"10.1109/IWBP.2011.5954832","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954832","url":null,"abstract":"The development of an increasing variety of single-molecule techniques has provided remarkable insights on several biological processes. Recently, tremendous improvements have been achieved in the precision of localization of single fluorescent molecules, allowing localization and tracking of biomolecules at the nm level. In the present work, we describe a single-molecule assay, based on the combination of two different single-molecule techniques in the same experimental setup: nanometer-precision Fluorescence Imaging and optical Trapping (FIAT). A microfluidic chamber allows fast exchange of the sample buffer between two different buffer compositions. The main advantage of the FIAT assay is the possibility of detecting the position of a single fluorescently labeled biomolecule and characterize its dynamics of interaction with the substrate, while precisely controlling the mechanical properties of the substrate itself. These features make FIAT well suitable for the study of several biological systems, including DNA-binding proteins and molecular motors. Here, we present preliminary results obtained with two proteins: RNA polymerase (RNAp) and the lactose repressor (LacI): two crucial proteins involved in prokaryotic gene expression and its regulation. RNAp, in a stalled ternary complex, was labeled with a quantum dot and localized on the T7 promoter. The DNA molecule containing the promoter was suspended between two optical traps and the position of RNAp was measured with a precision of ∼ 4 nm. For the study of LacI, the protein is labeled with a quantum dot through a genetically-encoded biotin tag at the C-terminal (after the tetramerization domain) and a DNA construct containing two primary operators (O1) is suspended between the two traps. The positions at which binding of LacI takes place are measured. These methods will be extended to the study of dynamics of RNAp and LacI in different mechanical conditions.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115620252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Fluorescence detection of fibrillar proteins on silicon microstructures 硅微结构上纤维蛋白的荧光检测

2011 International Workshop on Biophotonics Pub Date : 2011-06-08 DOI: 10.1109/IWBP.2011.5954809

S. Merlo, F. Carpignano, G. Silva, G. Barillaro, S. Surdo, L. Strambini, G. Mazzini, S. Raimondi, M. Stoppini

{"title":"Fluorescence detection of fibrillar proteins on silicon microstructures","authors":"S. Merlo, F. Carpignano, G. Silva, G. Barillaro, S. Surdo, L. Strambini, G. Mazzini, S. Raimondi, M. Stoppini","doi":"10.1109/IWBP.2011.5954809","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954809","url":null,"abstract":"The biology and the structure of amyloid fibrils are under extensive investigation in many laboratories: they are co-causative agents of diseases such as Parkinson's and Alzheimer's. We are investigating the use of a silicon micromachined structure, fabricated by electrochemical etching, as a three-dimensional supporting matrix also suitable for optically monitoring the amyloid fibrils growth. The silicon device consists in a periodic array of silicon walls with high aspect-ratio. This periodic arrangement of silicon and air gives rise to one-dimensional hybrid photonic crystals, suitable for out-of-plane (top view) imaging but, potentially, also for in-plane label-free testing. Here, we present some preliminary results relative to fluorescence microscopy analysis performed to investigate the interaction among silicon microstructures and fibrillar proteins. Samples of the highly amyloidogenic variant of human β2-microglobulin (P32G β2-m) are deposited on flat silicon dice as well as inserted into the gaps of the micromachined silicon devices. After Thioflavin T labeling, a bright emission originating only from silicon devices where polymerized amyloid fibrils are present is observed.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"129 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123225062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plasmonic biosensor schemes with thermo-responsive hydrogel binding matrix 热响应型水凝胶结合基质等离子体生物传感器方案

2011 International Workshop on Biophotonics Pub Date : 2011-06-08 DOI: 10.1109/IWBP.2011.5954841

M. Toma, W. Knoll, J. Dostálek, A. Mateescu, U. Jonas

引用次数: 0

A localized surface plasmon sensor for early cancer detection 用于早期癌症检测的局部表面等离子体传感器

2011 International Workshop on Biophotonics Pub Date : 2011-06-08 DOI: 10.1109/IWBP.2011.5954842

F. Rohde, R. Porcar

引用次数: 3

Localized photocatalysis by Au-titania plasmonics 金钛等离子体的局部光催化

2011 International Workshop on Biophotonics Pub Date : 2011-06-08 DOI: 10.1109/IWBP.2011.5954856

Lorenzo Rosa, S. Juodkazis, E. Kowalska

引用次数: 2

Extending the applicability of Raman microspectroscopy in biomedicine using statistical analysis and plasmonic effects 利用统计分析和等离子体效应扩大拉曼显微光谱在生物医学中的适用性

2011 International Workshop on Biophotonics Pub Date : 2011-06-08 DOI: 10.1109/IWBP.2011.5954816

Mónica Marro, Saurabh Raj, Satish Rao, D. Petrov

引用次数: 0