{"title":"Responses of glyoxalase system, ascorbate-glutathione cycle, and antioxidant enzymes in <i>Pontederia cordata</i> to lead stress and its capacity to remove lead.","authors":"Jianpan Xin, Sisi Ma, Runan Tian","doi":"10.1080/15226514.2024.2421362","DOIUrl":"https://doi.org/10.1080/15226514.2024.2421362","url":null,"abstract":"<p><p>A hydroponic experiment was conducted to investigate the variations in membrane permeabilities, chlorophyll contents, antioxidase activities, the ascorbic acid (AsA)-glutathione (GSH) cycle, and the glyoxalase system in the leaves of <i>Pontederia cordata</i> with 0 ∼ 15.0 mg L<sup>-1</sup> lead ion (Pb<sup>2+</sup>) exposure. The concentrations of Pb<sup>2+</sup> accumulated in the plant roots, stems, and leaves were also evaluated. After 7 days of exposure, the plants maintained normal growth, and there was a significant increase in ascorbate peroxidase and dehydroascorbate reductase activities. With 5.0 mg L<sup>-1</sup> Pb<sup>2+</sup> exposure for 28 days, nearly 66.36% of Pb<sup>2+</sup> accumulated in the roots, while excess Pb<sup>2+</sup> immobilized in the leaves was not observed. Exposure to 10.0 and 15.0 mg L<sup>-1</sup> Pb<sup>2+</sup> for 28 days significantly increased Pb<sup>2+</sup> contents in the leaves. This led to decrease in chlorophyll <i>a, b</i>, and carotenoid contents, and to increase in the methylglyoxal content in the leaves. With 10 and 15 mg L<sup>-1</sup> Pb<sup>2+</sup> exposure, NPT and PCs contents in leaves increased. however, the glyoxalase system did not function well in the plant tolerant to Pb<sup>2+</sup> at higher concentrations. The AsA-GSH cycle did not cooperate with the glyoxalase system in the plant defense against Pb<sup>2+</sup> exposure in the present investigation.</p>","PeriodicalId":14235,"journal":{"name":"International Journal of Phytoremediation","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142603714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Daniel G Coelho, Vinicius M Silva, Claudio S Marinato, Pedro H S Neves, Antonio A P Gomes Filho, Fernanda S Farnese, Wagner L Araújo, Juraci A Oliveira
{"title":"Hyperaccumulation of metal in the apoplast contributes to the tolerance of the phytoremediator <i>Pistia stratiotes</i> L. to manganese-contaminated water.","authors":"Daniel G Coelho, Vinicius M Silva, Claudio S Marinato, Pedro H S Neves, Antonio A P Gomes Filho, Fernanda S Farnese, Wagner L Araújo, Juraci A Oliveira","doi":"10.1080/15226514.2024.2422462","DOIUrl":"10.1080/15226514.2024.2422462","url":null,"abstract":"<p><p>Phytoremediation of manganese (Mn)-contaminated water requires the selection of Mn-tolerant species. This study reports on physiological changes and Mn bioaccumulation in the aquatic macrophyte <i>Pistia stratiotes</i> cultivated under various MnCl<sub>2</sub> concentrations: control, 80, 340, 600, 1000, 2000, and 4000 µM. Few visual symptoms of Mn toxicity, such as chlorosis, were observed after 10 days, especially in plants treated with 2000 and 4000 µM MnCl<sub>2</sub>. High Mn accumulation was recorded, with maximum values of 23,700 and 24,600 µg g<sup>-1</sup> DW in the shoots and roots, respectively, at 4000 µM Mn, contrasting with 825.01 and 1587.53 µg g<sup>-1</sup> DW in control plants. Cellular fractioning showed that Mn in shoots and roots was mainly associated with the cell wall, with approximately 90% of the Mn in roots detected in the apoplast. There were no significant changes in net CO<sub>2</sub> assimilation or respiratory rates after 5 and 10 days of Mn exposure. These results demonstrate that <i>P. stratiotes</i> is a Mn hyperaccumulator species with excellent phytoremediation potential, as shown by its high bioaccumulation capacity and its ability to maintain photosynthetic efficiency under Mn stress.</p>","PeriodicalId":14235,"journal":{"name":"International Journal of Phytoremediation","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"<i>In situ</i> bioaugmented phytoremediation of cadmium and crude oil co-contaminated soil by <i>Ocimum gratissimum</i> in association with PGPR <i>Micrococcus luteus</i> WN01.","authors":"Pem Choden, Toemthip Poolpak, Prayad Pokethitiyook, Kwang Mo Yang, Maleeya Kruatrachue","doi":"10.1080/15226514.2024.2415535","DOIUrl":"https://doi.org/10.1080/15226514.2024.2415535","url":null,"abstract":"<p><p>Heavy metals and petroleum oil are the two most important contaminants in the environment. Currently, phytoremediation is regarded as an effective and affordable solution that allows the attenuation of toxic pollutants through the use of plants. Not many studies are carried out regarding the use of aromatic plants capable of remediating soil that is co-contaminated by heavy metal and petroleum hydrocarbons. A pot experiment was conducted to investigate the influence of cadmium-resistant PGPR <i>Micrococcus luteus</i> on the phytoremediation efficiency of <i>Ocimum gratissimum</i> in Cd and petroleum co-contaminated soil. The plants were harvested after 60 days of treatment and their growth and biomass were determined. The accumulation of Cd in plant shoots and roots was determined. The residual petroleum hydrocarbon concentration during the 60 days of the phytoremediation experiment was determined using GC-FID. <i>O. gratissimum</i> with <i>M. luteus</i> showed the highest Cd accumulation (14.05 mg kg<sup>-1</sup>) and the highest reduction of TPH (46.64%). <i>M. luteus</i> ameliorated contaminant toxicity and promoted biomass production of <i>O. gratissimum</i>. These results demonstrated that <i>O. gratissimum</i> in combination with <i>M. luteus</i> can be efficiently used to remediate Cd and petroleum-co-contaminated soils.</p>","PeriodicalId":14235,"journal":{"name":"International Journal of Phytoremediation","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142583055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Parammal Faseela, Mathew Veena, Akhila Sen, K S Anjitha, K P Raj Aswathi, Palliyath Sruthi, Jos T Puthur
{"title":"Elicitors fortifies the plant resilience against metal and metalloid stress.","authors":"Parammal Faseela, Mathew Veena, Akhila Sen, K S Anjitha, K P Raj Aswathi, Palliyath Sruthi, Jos T Puthur","doi":"10.1080/15226514.2024.2420328","DOIUrl":"https://doi.org/10.1080/15226514.2024.2420328","url":null,"abstract":"<p><p>This review addresses plant interactions with HMs, emphasizing defence mechanisms and the role of chelating agents, antioxidants and various elicitor molecules in mitigating metal toxicity in plants. To combat soil contamination with HMs, chelate assisted phytoextraction using application of natural or synthetic aminopolycarboxylic acids is an effective strategy. Plants also employ diverse signaling pathways, including hormones, calcium, reactive oxygen species, nitric oxide, and Mitogen-Activated Protein Kinases influencing gene expression and defence mechanisms to counter HM stress. Phytohormones enhance the enzymatic and non-enzymatic antioxidant defence mechanism and the level of secondary metabolites in plants when exposed to HM stress. Also it activates genes responsible for DNA repair mechanism. In addition, the plant hormones can also regulate the activity of several transporters of HMs, thereby preventing their entry into the cell. Elicitor molecules regulate metal and metalloid absorption, sequestration and transport in plants. Combining of different elicitors like jasmonic acid, calcium, salicylic acid etc. effectively mitigates metal and metalloid stress in plants. Moreover, microbes including bacteria and fungi, offer eco-friendly and efficient solution for HM remediation. Understanding these elicitors, microbes and various signaling pathways is crucial for developing strategies to enhance plant resilience to metal and metalloid stress.</p>","PeriodicalId":14235,"journal":{"name":"International Journal of Phytoremediation","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142567308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Morish Azabo, Amal Abdelhaleem, Manabu Fujii, Mahmoud Nasr
{"title":"<i>Pontederia crassipes</i> utilization for dual phytoremediation and adsorption in greywater treatment: a techno-economic and sustainable approach.","authors":"Morish Azabo, Amal Abdelhaleem, Manabu Fujii, Mahmoud Nasr","doi":"10.1080/15226514.2024.2374887","DOIUrl":"10.1080/15226514.2024.2374887","url":null,"abstract":"<p><p>While phytoremediation has been widely employed for greywater treatment, this system suffers from the transfer of considerable amounts of surfactants to the aquatic environment through partially treated effluent and/or exhausted plant disposal. Hence, this study focuses on greywater phytoremediation followed by recycling the spent plant for preparing an adsorbent material used as post-treatment. <i>P. crassipes</i> was used to operate a phytoremediation unit under 23 °C, 60% relative humidity, plant density (5-30 g/L), dilution (0-50%), pH (4-10), and retention time (3-15 days). The optimum condition was 12.7 g/L density, 34.0% dilution, pH 8.4, and 13 days, giving chemical oxygen demand (COD), surfactant, and NH<sub>4</sub>-N removal efficiencies of 94.62%, 90.45%, and 88.09%, respectively. The exhausted plant was then thermally treated at 550 °C and 40 min to obtain biochar used as adsorbent to treat the phytoremediation effluent. The optimum adsorption process was biochar dosage of 1.51 g/L, pH of 2.1, and 137 min, providing a surfactant removal efficiency of 92.56%. The final discharge of this phytoremediation/adsorption combined process contained 8.30 mg/L COD, 0.23 mg/L surfactant, and 0.94 mg/L NH<sub>4</sub><sup>+</sup>-N. Interestingly, this approach could be economically feasible with a payback period of 6.5 years, 14 USD net present value, and 8.6% internal rate of return.</p>","PeriodicalId":14235,"journal":{"name":"International Journal of Phytoremediation","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141534430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jian Yang, Zuyong Chen, Jie Dai, Fang Liu, Jian Zhu
{"title":"Research on the optimal ratio of improved electrolytic manganese residue substrate about <i>Pennisetum sinese Roxb</i> growth effects.","authors":"Jian Yang, Zuyong Chen, Jie Dai, Fang Liu, Jian Zhu","doi":"10.1080/15226514.2024.2379610","DOIUrl":"10.1080/15226514.2024.2379610","url":null,"abstract":"<p><p>Electrolytic manganese slag (EMR) is a solid waste generated in the manganese hydrometallurgy process. It not only takes up significant land space but also contains Mn<sup>2+</sup>, which can lead to environmental contamination. There is a need for research on the treatment and utilization of EMR. Improved EMR substrate for <i>Pennisetum sinese Roxb</i> growth was determined in pot planting experiments. The study tested the effects of leaching solution, microorganisms, leaf cell structures, and growth data. Results indicated a substrate of 45% EMR, 40% phosphogypsum, 5% <i>Hericium erinaceus</i> fungi residue, 5% quicklime, and 5% dolomite sand significantly increased the available phosphorus content (135.54 ± 2.88 μg·g<sup>-1</sup>) by 17.95 times, compared to pure soil, and enhanced the relative abundance of dominant bacteria. After 240 days, the plant height (147.00 ± 0.52 cm), number of tillers (6), and aerial dry weight (144.00 ± 15.99g) of <i>Pennisetum sinese Roxb</i> increased by 5.81%, 200%, and 32.58%, respectively. Analyses of leaves and leaching solution revealed that the highest leaf Mn content (46.84 ± 2.91 μg·g<sup>-1</sup>) being 3.38 times higher than in pure soil, and the leaching solution Mn content (0.66 ± 0.13 μg·g<sup>-1</sup>) was lowest. Our study suggested <i>P. sinese Roxb</i> grown in an improved EMR substrate could be a feasible option for solidification treatment and resource utilization of EMR.</p>","PeriodicalId":14235,"journal":{"name":"International Journal of Phytoremediation","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141758614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Denisse Astrid Hernández-Castelán, Florentina Zurita, Oscar Marín-Peña, Erick Arturo Betanzo-Torres, Mayerlin Sandoval-Herazo, Jesús Castellanos-Rivera, Luis Carlos Sandoval Herazo
{"title":"Effect of monocultures and polycultures of <i>Typha latifolia</i> and <i>Heliconia psittacorum</i> on the treatment of river waters contaminated with landfill leachate/domestic wastewater in partially saturated vertical constructed wetlands.","authors":"Denisse Astrid Hernández-Castelán, Florentina Zurita, Oscar Marín-Peña, Erick Arturo Betanzo-Torres, Mayerlin Sandoval-Herazo, Jesús Castellanos-Rivera, Luis Carlos Sandoval Herazo","doi":"10.1080/15226514.2024.2379007","DOIUrl":"10.1080/15226514.2024.2379007","url":null,"abstract":"<p><p>Partially Saturated Vertical Constructed Wetlands (PSV-CWs) are novel wastewater treatment systems that work through aerobic and anaerobic conditions that favor the removal of pollutants found in high concentrations, such as rivers contaminated with domestic wastewater and landfill leachate. The objective of the study was to evaluate the efficiency of PSV-CWs using monocultures and polycultures of <i>Typha latifolia</i> and <i>Heliconia psittacorum</i> to treat river waters contaminated with leachates from open dumps and domestic wastewater. Six experimental units of PSV-CWs were used; two were planted with <i>Typha latifolia</i> monoculture, two with <i>Heliconia psittacorum</i> monoculture and two with polycultures of both plants. The results indicated better organic matter and nitrogen removal efficiencies (<i>p</i> < 0.05) in systems with polycultures (TSS:95%, BOD<sub>5</sub>:83%, COD:89%, TN:82% and NH<sub>4+</sub>:99%). In general, the whole system showed high average removal efficiencies (TSS:93%, BOD<sub>5</sub>:79%, COD:85%, TN:79%, NH<sub>4+</sub>:98% and TP:85%). Regarding vegetation, both species developed better in units with monocultures, being <i>Typha latifolia</i> the one that reached a more remarkable development. However, both species showed high resistance to the contaminated environment. These results showed higher removals than those reported in the literature with conventional Free Flow Vertical Constructed Wetlands (FFV-CWs), so PSV-CWs could be a suitable option to treat this type of effluent.</p>","PeriodicalId":14235,"journal":{"name":"International Journal of Phytoremediation","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141590314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Spontaneous growth of plants enhances phytoextraction on abandoned coal mine wastes in Central Alborz coalfield, Iran.","authors":"Nateq Lashkari Sanami, Jamshid Ghorbani, Ghorban Vahabzadeh, Seyed Mohammad Hodjati, Babak Motesharezadeh","doi":"10.1080/15226514.2024.2378217","DOIUrl":"10.1080/15226514.2024.2378217","url":null,"abstract":"<p><p>Coal mining disperses heavy metals into the environment, necessitating the identification of metal-tolerant plants for ecosystem restoration. This study evaluated the phytoremediation potential of plant species in abandoned coal wastes in northern Iran. Pollution indices indicated moderate contamination of Cu, Ni, V, Zn, Pb, Cr, and As in coal wastes. The plants varied in their ability to accumulate and translocate these metals, with most showing efficient root-to-shoot translocation. <i>Artemisia scoparia</i> (41.06 mg.kg<sup>-1</sup>) and <i>Capparis spinosa</i> (42.48 mg.kg<sup>-1</sup>) were effective for Cu phytoextraction. Most species, notably <i>Cynodon dactylon</i> (3.4 mg.kg<sup>-1</sup>), showed promise for phytoextraction of Cr. <i>Capparis spinosa</i> (7.67 mg.kg<sup>-1</sup>) exhibited potential for Pb phytoextraction. Most plants, particularly <i>Hordeum vulgare</i> and <i>Melica persica</i>, were effective phytoextractors of Ni. <i>Sylibum marianum</i> accumulated V beyond phytotoxic levels. <i>Chenopodium album</i> and <i>Glaucium fimbriligerum</i> were identified as phytoextractors of Zn while <i>Cynodon dactylon</i> and <i>Hordeum vulgare</i>, accumulating >100 mg.kg<sup>-1</sup> Zn in roots, showed potential for phytostabilization. <i>Sylibum marianum</i> and <i>Glaucium fimbriligerum</i>, acted as excluders for As. <i>Kochia prostrata</i> and <i>Artemisia aucheri</i> were excluders for Cu, Cr, Ni, and Pb. This study provided the role of multiple indigenous plants, including perennials and annuals with diverse life forms, in metal extraction and stabilization for sustainable coal waste management.</p>","PeriodicalId":14235,"journal":{"name":"International Journal of Phytoremediation","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141599869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Soil amendment-assisted phytoremediation with ryegrass offers a promising approach to mitigate environmental health concerns.","authors":"Albert Kobina Mensah","doi":"10.1080/15226514.2024.2380039","DOIUrl":"10.1080/15226514.2024.2380039","url":null,"abstract":"<p><p>This study aimed to examine the potential of soil amendment-assisted phytoremediation using ryegrass in reclaiming abandoned gold mine soil in southwestern Ghana, with a specific focus on the soil contamination hazards associated with metals and metalloids. A pot experiment lasting 60 days was carried out to assess the efficacy of soil amendments, such as compost, iron oxide, and poultry manure, in mitigating environmental hazards. Three soil contamination indices (soil contamination = CF, enrichment factor = ER, and pollution load index = PLI) were used to calculate the extent of soil contamination, enrichment, and pollution of the sites with Co, Hg, Ni, Mo, Se, Sb, and Pb. The findings show that Hg made the greatest contribution (with a maximum soil CF of 18.0) to the overall PLI, with a maximum value of 74.4. The sites were averagely and consequently enriched with toxic elements in the decreasing order: Ni (ER = 33.3) > Mo (20.5) > Sb (14.1) > Pb (11.0) > Hg (7.9) > Se (2.1). The bioaccumulation factor (BCF > 1) suggests that ryegrass has the ability to phytostabilize Co, Hg, Mo, and Ni. This means that the plant may store these elements in its roots, potentially decreasing their negative effects on the environment and human health. Ultimately, the addition of combined manure with iron oxides might have augmented the sequestration of these metals in the root. The elements may have accumulated through sorption on manure or Fe surfaces, dissolution from watering the plants in the pot, or mineralization of organic manure. Thus, ryegrass has shown potential for phytostabilisation of Co, Hg, Mo, and Ni when assisted with a combination of manure and iron oxides; and can consequently mitigate the environmental and human health impacts.</p>","PeriodicalId":14235,"journal":{"name":"International Journal of Phytoremediation","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jiangbo Guo, Hanyang Liu, Yang Xu, Lu Li, Cuihua Xin
{"title":"Ectopic expression of the yeast Mn<sup>2+</sup> transporter <i>SMF2</i> enhances tolerance and resistance to cadmium and arsenic in transgenic Arabidopsis.","authors":"Jiangbo Guo, Hanyang Liu, Yang Xu, Lu Li, Cuihua Xin","doi":"10.1080/15226514.2024.2373974","DOIUrl":"10.1080/15226514.2024.2373974","url":null,"abstract":"<p><p>Vesicular sequestration is a potential strategy for enhancing plant tolerance to cadmium (Cd) and arsenic (As). In this study, the ectopic overexpression of yeast-derived <i>ScSMF2</i> in <i>Arabidopsis thaliana</i> was found to enhance the accumulation and tolerance of Cd and As in transgenic plants. ScSMF2 was localized on vacuole membranes and formed puncta structures in plant cells when agro-infiltrated for transient expression. Transgenic Arabidopsis showed less retardation on root elongation and shoot weight and more accumulation of Cd, As (III) and As (V) when cultured on medium containing Cd or As. Overexpression of <i>ScSMF2</i> promoted accumulation of Cd and arsenic in transgenic Arabidopsis, which were over twice higher than in WT plants when cultured in soil. This study provides insights into the mechanisms involved in the vesicular sequestration of heavy metals in plant and presents a potential strategy for enhancing the phytoremediation capacity of plants toward heavy metals.</p>","PeriodicalId":14235,"journal":{"name":"International Journal of Phytoremediation","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141554817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}