International Journal of Peptide Research and Therapeutics最新文献_第6页

Systematic Profiling of Mitogen-Inducible Gene 6 and Its Derived Peptides Binding to Receptor Tyrosine Kinases in Bone Cancers at Molecular and Cellular Levels 从分子和细胞水平系统分析与骨癌受体酪氨酸激酶结合的丝裂原诱导基因 6 及其衍生肽

IF 2.5 4区生物学

International Journal of Peptide Research and Therapeutics Pub Date : 2024-01-04 DOI: 10.1007/s10989-023-10585-4

Jinping Ni, Zhidong Zhong, Weikang Lu, Shuai Li, Xiang Shao, Lihua Hang

{"title":"Systematic Profiling of Mitogen-Inducible Gene 6 and Its Derived Peptides Binding to Receptor Tyrosine Kinases in Bone Cancers at Molecular and Cellular Levels","authors":"Jinping Ni, Zhidong Zhong, Weikang Lu, Shuai Li, Xiang Shao, Lihua Hang","doi":"10.1007/s10989-023-10585-4","DOIUrl":"https://doi.org/10.1007/s10989-023-10585-4","url":null,"abstract":"Mitogen-inducible gene 6 (Mig6) is a tumor suppressor that inactivates oncogenic kinases by disrupting the dimerization of kinase domain using its segment 1 (SGT1). Traditionally, Mig6 has been documented as a cognate binder of the ErbB family of receptor tyrosine kinases (RTKs). Considering that the kinase domains of RTKs are highly conserved that share significant homology in sequence, structure and function, we herein assumed that the Mig6 SGT1 should not only bind to ErbBs, but can also target and then inhibit other RTKs in bone cancers and bone cancer pain. In this study, we systematically profiled the binding behavior of Mig6 SGT1 segment peptide against various ontology-enriched RTKs that are semantically relevant with bone cancers. A variety of RTKs were computationally identified as the potential binding hits of Mig6 SGT1; most of them have been previously reported to be involved in the cell signaling pathways of bone cancer pathogenesis. Binding analysis further revealed that the VEGFR1 and VEGFR2 kinases are two potent binders of Mig6 SGT1, which are comparable with or even stronger than the EGFR, while other two FGFR1 and FGFR2 kinases were also observed to have a moderate potency to Mig6 SGT1. Interaction modeling and dynamics simulation revealed that the full-length Mig6 SGT1 segment contains three hotspot sub-peptide fragments sbpt1, sbpt2 and sbpt3, which are primarily responsible for SGT1 binding to RTKs and can interact with the kinase dimerization interface in an independent manner. The sbpt2 was found as a moderately potent binder of VEGFR1 kinase domain at molecular level, while the Mig6 SGT1 and its derived sbpt1 and sbpt2 were observed to have similar suppressing effects on human osteosarcoma at cellular level.","PeriodicalId":14217,"journal":{"name":"International Journal of Peptide Research and Therapeutics","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139101812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design and Production of a Chimeric Enzyme with Efficient Antibacterial Properties on Staphylococcus aureus 设计和生产对金黄色葡萄球菌具有高效抗菌特性的嵌合酶

IF 2.5 4区生物学

International Journal of Peptide Research and Therapeutics Pub Date : 2024-01-02 DOI: 10.1007/s10989-023-10584-5

Amin M. Saber, Hossein Aghamollaei, Hadi Esmaeili Gouvarchin Ghaleh, Mozafar Mohammadi, Said Yaghoob Sehri, Gholamreza Farnoosh

{"title":"Design and Production of a Chimeric Enzyme with Efficient Antibacterial Properties on Staphylococcus aureus","authors":"Amin M. Saber, Hossein Aghamollaei, Hadi Esmaeili Gouvarchin Ghaleh, Mozafar Mohammadi, Said Yaghoob Sehri, Gholamreza Farnoosh","doi":"10.1007/s10989-023-10584-5","DOIUrl":"https://doi.org/10.1007/s10989-023-10584-5","url":null,"abstract":"Improper use of antibiotics has alarmingly led to the emergence of antibiotic resistance. Hence, this necessitated an urgent need to find a suitable alternative to traditional antibiotics. Endolysins are enzymes produced at the end of the phage replication cycle and destroy the peptidoglycan of the bacterial cell wall leading to the lysis of the host bacterial cell. These enzymes are species-specific, exhibit high lytic activity, and it is almost impossible for bacteria to develop resistance against them. Lysozyme subfamily 2 (LYZ2) is a modular region of the gene 61 (gp61) of phage φMR11 with lytic activity against S. aureus. However, it does not possess a cell wall recognition domain, which is usually found in lysins acting against gram-positive bacteria. Therefore, in this study, we engineered the LYZ2 by fusing a Staphylococcus aureus cell wall-binding domain (CBD) to its C-terminus and cloned the chimeric protein (named chimeric Staphylococcus aureus–targeting enzybiotic (CSTEnz)) into the pET28a vector, and expressed the enzyme in E. coli BL21 (DE3) cell. The engineered lysin displayed a rapid and specific lytic activity against susceptible and Methicillin-resistant Staphylococcus aureus and inhibited the growth of the bacteria at concentrations higher than 0.5 µg/ml. Besides, the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of CSTEnz were both approximately 64 times lower than those of LYZ2, indicating the increased bacteriolytic activity of the engineered version of the enzyme. In conclusion, the chimeric enzybiotic can be used as a potential antibacterial agent to limit infections caused by methicillin-resistant Staphylococcus aureus (MRSA).","PeriodicalId":14217,"journal":{"name":"International Journal of Peptide Research and Therapeutics","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139077790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alleviation of LPS-induced Endothelial Injury due to GHRH Antagonist Treatment. GHRH 拮抗剂可缓解 LPS 诱导的内皮损伤。

IF 2 4区生物学

International Journal of Peptide Research and Therapeutics Pub Date : 2024-01-01 Epub Date: 2024-10-01 DOI: 10.1007/s10989-024-10653-3

Saikat Fakir, Khadeja-Tul Kubra, Mohammad Shohel Akhter, Mohammad Afaz Uddin, Nektarios Barabutis

{"title":"Alleviation of LPS-induced Endothelial Injury due to GHRH Antagonist Treatment.","authors":"Saikat Fakir, Khadeja-Tul Kubra, Mohammad Shohel Akhter, Mohammad Afaz Uddin, Nektarios Barabutis","doi":"10.1007/s10989-024-10653-3","DOIUrl":"10.1007/s10989-024-10653-3","url":null,"abstract":"Background: GHRH is produced in the hypothalamus and affects various tissues beyond the pituitary, including the lungs. GHRH antagonists exert anti-inflammatory properties in several experimental models of disease, but their role inprotecting the endothelial barrier during inflammation is less understood. This study investigates the effects ofGHRHAnt on LPS-induced endothelial dysfunction.Methods: BPAEC and HMVEC-L cells were exposed to LPS to induce endothelial injury. GHRHAnt was administered eitherpre- or post-LPS treatment. Western blot analysis was used to evaluate protein expression levels. Paracellularpermeability was assessed utilizing FITC-dextran assay to evaluate endothelial barrier function.Results: GHRHAnt post-treatment significantly reduced LPS-induced MLC2 phosphorylation and cofilin activation inBPAECs. Furthermore, pretreatment with GHRHAnt enhanced barrier function and ameliorated LPS-inducedhyperpermeability in both human and bovine endothelial cells.Conclusions: GHRHAnt treatment mitigates LPS-induced endothelial barrier dysfunction. These findings suggest that GHRHAntcould serve as potential therapeutic agents towards endothelial dysfunction-related illness (e.g. sepsis).","PeriodicalId":14217,"journal":{"name":"International Journal of Peptide Research and Therapeutics","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11500629/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142500472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Types and Applications of Peptibodies 蛋白胨抗体的类型和应用

IF 2.5 4区生物学

International Journal of Peptide Research and Therapeutics Pub Date : 2023-12-27 DOI: 10.1007/s10989-023-10582-7

Mohammadmahdi Nemati, Ahmadreza Ahmadi, Ahmad Hashemzehi, Farukhruzi Nasrullozoda, Mohsen Abedi, Masoud Hashemzaei

引用次数: 0

Scaled-up Synthesis and Characterization of Oxytocin Trisulfide 三硫化催产素的放大合成与表征

IF 2.5 4区生物学

International Journal of Peptide Research and Therapeutics Pub Date : 2023-12-26 DOI: 10.1007/s10989-023-10580-9

Robert P. Hammer, Melissa A. Butrie, Karen Davidson, Phillip T. Goldblatt, Alex M. Schrader, Joseph J. Dalluge, Allyn Becker, George Barany

引用次数: 0

Evaluation of the Antimicrobial Efficacy of Epidermal Mucus Extract from Air-Breathing Fish (Channa punctatus) and Identification of the Peptides Serving as Immune Components 评估呼吸空气鱼（鳢）表皮粘液提取物的抗菌功效并鉴定作为免疫成分的多肽

IF 2.5 4区生物学

International Journal of Peptide Research and Therapeutics Pub Date : 2023-12-18 DOI: 10.1007/s10989-023-10581-8

Ahmed Hussain, Shashwati Ghosh Sachan

{"title":"Evaluation of the Antimicrobial Efficacy of Epidermal Mucus Extract from Air-Breathing Fish (Channa punctatus) and Identification of the Peptides Serving as Immune Components","authors":"Ahmed Hussain, Shashwati Ghosh Sachan","doi":"10.1007/s10989-023-10581-8","DOIUrl":"https://doi.org/10.1007/s10989-023-10581-8","url":null,"abstract":"Fish and other members of aquatic habitats are in constant contact with the microbes that trigger their immune system to produce immune components along with mucus. Compounds like lysozymes, proteases, lectins and peroxidase were reported in many studies. The study aims to identify and characterize the immune components present in the epidermal mucus of Channa punctatus. For this, antibacterial and antifungal efficacy was tested first using agar well diffusion assay and MIC was measured. Maximum activity against bacteria’s E. coli, S. aureus and Fungus H. gramineum was observed. FESEM analysis was performed to reveal the mechanism of the antimicrobial activity. Physico–chemical stability of mucus was also checked by exposing to various parameters such as (temperature, pH, proteases and surfactants). Precipitated proteins of mucus were separated using 12% SDS-PAGE assay. The band was digested using trypsin digestion and digested peptides were subjected to LCMSMS analysis for identification and on characterization using MASCOT servers 2 proteins (Hemoglobin subunit beta-A-globin chain and Galactose specific lectins nattectin) with number of fragmented unique peptides were obtained. Insilico predictions of antibacterial, antifungal, antiviral and anticancerous properties of peptides were also determined to support the role of mucus in microbial defense and therapeutical applications using the servers AMPfun, DBAASP and APD3.","PeriodicalId":14217,"journal":{"name":"International Journal of Peptide Research and Therapeutics","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138714452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design and Self Assembly of Tri-Terpene Peptide Conjugates and Their Interactions with EGFR and EGFR Mutant Receptors: An In Silico and In Vitro Study 三萜肽共轭物的设计和自组装及其与表皮生长因子受体和表皮生长因子受体突变受体的相互作用：硅学和体外研究

IF 2.5 4区生物学

International Journal of Peptide Research and Therapeutics Pub Date : 2023-12-14 DOI: 10.1007/s10989-023-10583-6

Mia I. Rico, Beatriz G. Goncalves, Hannah L. Hunt, Ipsita A. Banerjee

{"title":"Design and Self Assembly of Tri-Terpene Peptide Conjugates and Their Interactions with EGFR and EGFR Mutant Receptors: An In Silico and In Vitro Study","authors":"Mia I. Rico, Beatriz G. Goncalves, Hannah L. Hunt, Ipsita A. Banerjee","doi":"10.1007/s10989-023-10583-6","DOIUrl":"https://doi.org/10.1007/s10989-023-10583-6","url":null,"abstract":"In this work, we designed new terpenoid-peptide conjugates to target the epidermal growth factor receptor (EGFR) and its double mutant EGFR T790M/L858R which are implicated in many cancers. The peptides utilized were MEGPSKCCFSLALSH (MFSL), a new peptide sequence designed by mutating an ErbB2 targeting peptide, while the sequence VPWXE was derived from a peptide motif known to target tumor cells. Each of the peptides were conjugated to four terpenoids, 23-hydroxy betulinic acid (HB), oleanolic acid, perillic acid, and ursolic acid. Molecular docking and molecular dynamics (MD) simulations with the kinase domain of both the wild type and double mutant EGFR receptors revealed that the conjugates formed H-bonds and hydrophobic interactions with key residues in the hinge region, A-loop, and DFG motif, while in the case of the double mutant, interactions also occurred with C-terminal residues and with allosteric sites. MMGBSA analysis revealed higher binding energies for the double mutant. Six of the conjugates were synthesized and self-assembled into nanoassemblies and their impact on tumor cells expressing the wild type and double mutant receptor revealed that higher apoptosis was induced by MFSL conjugates, particularly in cells expressing the double mutant EGFR receptor. The HB and ursolate conjugates were found to be more potent against the tumor cell lines. Overall, these results indicate that these peptides and peptide conjugates can effectively bind to both the wild type and the T790M/L858R receptors to target tumor cells. Such peptide conjugates may be further potentially developed as therapeutic agents for further laboratory studies against tumors overexpressing EGFR.","PeriodicalId":14217,"journal":{"name":"International Journal of Peptide Research and Therapeutics","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138628790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Review of the in Silico Design and Development Approaches of Ras-Specific Anticancer Therapeutics ras特异性抗癌药物的芯片设计与开发方法综述

IF 2.5 4区生物学

International Journal of Peptide Research and Therapeutics Pub Date : 2023-12-02 DOI: 10.1007/s10989-023-10578-3

Parinaz Motiei, Hamid Reza Heidari, Mohammad Saeid Hejazi, Ommoleila Molavi, Elnaz Mehdizadeh Aghdam

{"title":"A Review of the in Silico Design and Development Approaches of Ras-Specific Anticancer Therapeutics","authors":"Parinaz Motiei, Hamid Reza Heidari, Mohammad Saeid Hejazi, Ommoleila Molavi, Elnaz Mehdizadeh Aghdam","doi":"10.1007/s10989-023-10578-3","DOIUrl":"https://doi.org/10.1007/s10989-023-10578-3","url":null,"abstract":"Ras Proteins, play a pivotal role in the proliferation pathways, and Ras mutant forms are well-established cancer drivers. Ras mutations are found in about 30% of all human cancers widely known as challenging diseases to control and treat. The direct inhibition of Ras is challenging and makes Ras an “undruggable” target for many years. The important reason is, that Ras protein has a unique smooth surface and shows different dynamicity upon binding GDP and GTP. As a result, interfering peptides (IPs) targeting the Ras family protein-protein interactions (PPIs) are considered more likely to bind Ras effectively and inhibit the downstream signaling. In this review, we aimed to cover the recent approaches to design the peptides that target Ras family proteins, focusing on in silico methods. In this regard, the anti-cancer peptide development approaches including design and delivery strategies are discussed. Later, more specific methods regarding Ras-specific peptide design are presented. In conclusion, IPs are a promising group of cancer therapeutics to combat Ras mutant cancers. For future perspectives to have these peptides in clinical use, co-inhibition of other cancer targets as well as improving the pharmacokinetic features of peptides are suggested.","PeriodicalId":14217,"journal":{"name":"International Journal of Peptide Research and Therapeutics","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138518603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytotoxic Activity of A New Isoform l-Amino Acid Oxidase (Balt-LAAO-II) From Bothrops alternatus (Urutu) Snake Venom in Human Leukemic HL60 Cells 一种新的异型l-氨基酸氧化酶(Balt-LAAO-II)在人白血病HL60细胞中的细胞毒活性

IF 2.5 4区生物学

International Journal of Peptide Research and Therapeutics Pub Date : 2023-11-22 DOI: 10.1007/s10989-023-10574-7

Mauricio Aurelio Gomes Heleno, Alexandre Nowill, João Ernesto de Carvalho, Diego L. Suni-Curasi, Julissa Vilca-Quispe, Emilio Alberto Ponce-Fuentes, Gustavo Alberto Obando-Pereda, Luis Alberto Ponce-Soto

{"title":"Cytotoxic Activity of A New Isoform l-Amino Acid Oxidase (Balt-LAAO-II) From Bothrops alternatus (Urutu) Snake Venom in Human Leukemic HL60 Cells","authors":"Mauricio Aurelio Gomes Heleno, Alexandre Nowill, João Ernesto de Carvalho, Diego L. Suni-Curasi, Julissa Vilca-Quispe, Emilio Alberto Ponce-Fuentes, Gustavo Alberto Obando-Pereda, Luis Alberto Ponce-Soto","doi":"10.1007/s10989-023-10574-7","DOIUrl":"https://doi.org/10.1007/s10989-023-10574-7","url":null,"abstract":"In this study, we describe the isolation of a new isoform, l-Amino acid oxidase (LAAO), referred to as Balt-LAAO-II, from Bothrops alternatus snake venom, which was highly purified using a combination of molecular exclusion (Sephadex G-75) and RP-HPLC chromatographic steps. SDS-PAGE analysis showed that purified Balt-LAAO-II had a molecular weight of (sim )66 kDa. The N-terminal amino acid sequence and internal peptide sequences showed close structural homology to those of other snake venom l-Amino acid oxidases. This enzyme induces in vitro cytotoxicity in cultured human leukemic HL60 cells. Cells were grown in RPMI medium and incubated with the isoform Balt-LAAO-II (1, 10, and 100 (mu )g/mL) for up to 72 h. All three concentrations of venom markedly decreased cell viability from 6 h onwards based on staining with propidium iodide, the reduction of 3-(4,5-dimethylthazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), and the uptake of neutral red. Flow cytometry showed that all isoforms of Balt-LAAO-II and whole venom concentrations induced apoptosis after 2–6 h of incubation. Morphological analysis of cells incubated with the isoform Balt-LAAO-II and whole venom showed cell rounding and lysis, which increased with venom concentration and duration of incubation. These results show that the isoform Balt-LAAO-II from venom Bothrops alternatus is cytotoxic to cultured HL60 cells, suggesting that this damage may involve the apoptotic and oxidative stress pathways.","PeriodicalId":14217,"journal":{"name":"International Journal of Peptide Research and Therapeutics","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138518602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Peptidomic Analysis and Potential Bioactive Peptides Identification Targeting Endometrial Cancer 针对子宫内膜癌的肽组学分析及潜在生物活性肽鉴定

4区生物学

International Journal of Peptide Research and Therapeutics Pub Date : 2023-11-13 DOI: 10.1007/s10989-023-10573-8

Hanzi Xu, Shenglong Yuan, Lin Zhou, Huixin Li, Juan Lv, Xiaoyan Shi, Wangfei Wu, Zhijian Feng, Zhen Gong

引用次数: 0