International Journal of Oral Biology最新文献_第5页

Expression of neurotransmitter receptors in oral keratinocytes and their response to agonists 神经递质受体在口腔角质形成细胞中的表达及其对激动剂的反应

International Journal of Oral Biology Pub Date : 2021-03-31 DOI: 10.11620/IJOB.2021.46.1.39

Eun Ji Choi, Sung-Ho Chang, Se-Young Choi, Youngnim Choi

引用次数: 0

The antibacterial effect of Endoseal TCS mixed with water-soluble mangostin derivatives of Garcinia mangostana L. ethanol extract against Enterococcus faecalis and Staphylococcus aureus Endoseal TCS与山竹果水溶性山竹苷衍生物乙醇提取物混合对粪肠球菌和金黄色葡萄球菌的抑菌效果

International Journal of Oral Biology Pub Date : 2021-03-31 DOI: 10.11620/IJOB.2021.46.1.45

Tae-Young Park, Y. Lim, Jin Hee Kim, Dae Sung Lee, J. Kook

引用次数: 0

Anticancer effects of D-pinitol in human oral squamous carcinoma cells d -蒎醇对人口腔鳞状癌细胞的抗癌作用

International Journal of Oral Biology Pub Date : 2020-12-30 DOI: 10.11620/IJOB.2020.45.4.152

Hyunjin Shin, T. Bang, Kang haemi, Park，Bong Soo, Kim Inryoung

{"title":"Anticancer effects of D-pinitol in human oral squamous carcinoma cells","authors":"Hyunjin Shin, T. Bang, Kang haemi, Park，Bong Soo, Kim Inryoung","doi":"10.11620/IJOB.2020.45.4.152","DOIUrl":"https://doi.org/10.11620/IJOB.2020.45.4.152","url":null,"abstract":"D-pinitol is an analog of 3-methoxy-D-chiro-inositol found in beans and plants. D-pinitol has anti-inflammatory, antidiabetic, and anticancer effects. Additionally, D-pinitol induces apoptosis and inhibits metastasis in breast and prostate cancers. However, to date, no study has investigated the anticancer effects of D-pinitol in oral cancer. Therefore, in this study, whether the anticancer effects of D-pinitol induce apoptosis, inhibit the epithelialto- mesenchymal transition (EMT), and arrest cell cycle was investigated in squamous epithelial cells. D-pinitol decreased the survival and cell proliferation rates of CAL-27 and Ca9-22 oral squamous carcinoma cells in a concentration- and time-dependent manner. Evidence of apoptosis, including nuclear condensation, poly (ADP-ribose) polymerase, and caspase-3 fragmentation, was also observed. D-pinitol inhibited the migration and invasion of both cell lines. In terms of EMT-related proteins, E-cadherin was increased, whereas N-cadherin, Snail, and Slug were decreased. D-pinitol also decreased the expression of cyclin D1, a protein involved in the cell cycle, but increased the expression of p21, a cyclin-dependent kinase inhibitor. Hence, D-pinitol induces apoptosis and cell cycle arrest in CAL-27 and Ca9-22 cells, demonstrating an anticancer effect by decreasing the EMT.","PeriodicalId":14180,"journal":{"name":"International Journal of Oral Biology","volume":"13 1","pages":"152-161"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79921220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Inhibitory effects of Coptis chinensis extract on the growth and biofilm formation of Streptococcus mutans and Streptococcus sobrinus 黄连提取物对变形链球菌和斜纹链球菌生长和生物膜形成的抑制作用

International Journal of Oral Biology Pub Date : 2020-12-30 DOI: 10.11620/IJOB.2020.45.4.143

S. Kim, Y. Song, H. A. Lee, H. Na, C. Jung, Gyung Yun Bek, Jin Chung

{"title":"Inhibitory effects of Coptis chinensis extract on the growth and biofilm formation of Streptococcus mutans and Streptococcus sobrinus","authors":"S. Kim, Y. Song, H. A. Lee, H. Na, C. Jung, Gyung Yun Bek, Jin Chung","doi":"10.11620/IJOB.2020.45.4.143","DOIUrl":"https://doi.org/10.11620/IJOB.2020.45.4.143","url":null,"abstract":"Streptococcus mutans and Streptococcus sobrinus play important roles in dental caries. Coptis chinensis is a natural product with antimicrobial activity against enterobacteria; however, its effects on oral streptococci are still unknown. Therefore, the effects of C. chinensis on the growth and biofilm formation of the representative cariogenic bacteria S. mutans and S. sobrinus were investigated for the possible use of C. chinensis as an anticaries agent. The C. chinensis extract was diluted with sterile distilled water, and 0.1–2.5% of the extract was used in the experiment. The effects of the C. chinensis extract on the growth and glucan formation of S. mutans and S. sobrinus were measured by viable cell counting and spectrophotometry at 650 nm absorbance, respectively. Crystal violet staining was also carried out to confirm the C. chinensis extract’s inhibitory effect on biofilm formation. The C. chinensis extract significantly inhibited the growth of S. mutans and S. sobrinus at concentrations of ≥ 0.3% as compared with the control group. The viable cell count of colonies decreased by 1.7-fold and 1.2-fold at 2.5% and 1.25%, respectively, compared with the control group. The biofilm formation of S. mutans and S. sobrinus was inhibited by > 20-fold at C. chinensis extract concentrations of ≥ 1.25% as compared with the control group. In summary, the C. chinensis extract inhibited the growth and biofilm and glucan formation of S. mutans and S. sobrinus . Therefore, C. chinensis might be a potential candidate for controlling dental caries.","PeriodicalId":14180,"journal":{"name":"International Journal of Oral Biology","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82119109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Induction of anti-aquaporin 5 autoantibodies by molecular mimicry in mice 分子模拟诱导小鼠抗水通道蛋白5自身抗体

International Journal of Oral Biology Pub Date : 2020-12-30 DOI: 10.11620/IJOB.2020.45.4.211

Ahreum Lee, Youngnim Choi

{"title":"Induction of anti-aquaporin 5 autoantibodies by molecular mimicry in mice","authors":"Ahreum Lee, Youngnim Choi","doi":"10.11620/IJOB.2020.45.4.211","DOIUrl":"https://doi.org/10.11620/IJOB.2020.45.4.211","url":null,"abstract":"Molecular mimicry is the most common mechanism that breaches self-tolerance. We previously identified autoantibodies to aquaporin-5 (AQP5) in the sera of patients with Sjogren’s syndrome and found that the aquaporin of Prevotella melaninogenica (PmAqp), an oral commensal, is highly homologous to human AQP5. This study aimed to test whether PmAqp can induce anti-AQP5 autoantibodies via molecular mimicry. From the amino acid sequence of PmAqp, an immunizing peptide; i.e., PmE-L, was designed, which contained both the B cell epitope “E” and T cell epitope. C57BL/6 and BALB/c mice were subcutaneously immunized with linear or cyclic forms of PmE-L emulsified in incomplete Freund’s adjuvant. The concentrations of the antibodies in sera were measured using enzymelinked immunosorbent assays. Both linear and cyclic PmE-L induced high levels of antibodies against not only the immunized peptides but also autoantibodies against AQP5E and antibodies against PmE, a Pm homolog of AQP5E. In C57BL/6 mice; however, the cyclic form of PmE-L was more efficient than the linear form in inducing autoantibodies against AQP5E that contained a cyclic epitope. The levels of anti-PmE antibodies and anti-AQP5E autoantibodies showed a strong positive correlation (r = 0.95, p < 0.0005), suggesting molecular mimicry. Collectively, the mice produced anti-AQP5E autoantibodies in response to a PmAqp-derived peptide. This model proved to be useful for studying the mechanisms of autoantibody production by molecular mimicry.","PeriodicalId":14180,"journal":{"name":"International Journal of Oral Biology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86384023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Alteration of cellular events in tooth development by chemical chaperon, Tauroursodeoxycholic acid treatment 化学伴侣子、金牛座脱氧胆酸处理对牙齿发育中细胞事件的改变

International Journal of Oral Biology Pub Date : 2020-12-30 DOI: 10.11620/IJOB.2020.45.4.190

Eui-Seon Lee, Y. P. Aryal, Tae-Young Kim, Elina Pokharel, Harim Kim, S. Sung, Wern-Joo Sohn, Lee Youngkyun, Chang-Hyeon An, K. J. Young

{"title":"Alteration of cellular events in tooth development by chemical chaperon, Tauroursodeoxycholic acid treatment","authors":"Eui-Seon Lee, Y. P. Aryal, Tae-Young Kim, Elina Pokharel, Harim Kim, S. Sung, Wern-Joo Sohn, Lee Youngkyun, Chang-Hyeon An, K. J. Young","doi":"10.11620/IJOB.2020.45.4.190","DOIUrl":"https://doi.org/10.11620/IJOB.2020.45.4.190","url":null,"abstract":"Several factors, including genetic and environmental insults, impede protein folding and secretion in the endoplasmic reticulum (ER). Accumulation of unfolded or mis-folded protein in the ER manifests as ER stress. To cope with this morbid condition of the ER, recent data has suggested that the intracellular event of an unfolded protein response plays a critical role in managing the secretory load and maintaining proteostasis in the ER. Tauroursodeoxycholic acid (TUDCA) is a chemical chaperone and hydrophilic bile acid that is known to inhibit apoptosis by attenuating ER stress. Numerous studies have revealed that TUDCA affects hepatic diseases, obesity, and inflammatory illnesses. Recently, molecular regulation of ER stress in tooth development, especially during the secretory stage, has been studied. Therefore, in this study, we examined the developmental role of ER stress regulation in tooth morphogenesis using in vitro organ cultivation methods with a chemical chaperone treatment, TUDCA. Altered cellular events including proliferation, apoptosis, and dentinogenesis were examined using immunostaining and terminal deoxynucleotidyl transferase dUTP nick end labeling assay. In addition, altered localization patterns of the formation of hard tissue matrices related to molecules, including amelogenin and nestin, were examined to assess their morphological changes. Based on our findings, modulating the role of the chemical chaperone TUDCA in tooth morphogenesis, especially through the modulation of cellular proliferation and apoptosis, could be applied as a supporting data for tooth regeneration for future studies.","PeriodicalId":14180,"journal":{"name":"International Journal of Oral Biology","volume":"126 1","pages":"190-196"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85108748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antitumor effects of octyl gallate on hypopharyngeal carcinoma cells 没食子酸辛酯对下咽癌细胞的抗肿瘤作用

International Journal of Oral Biology Pub Date : 2020-12-30 DOI: 10.11620/IJOB.2020.45.4.218

Trang Ntk, H. Yoo

引用次数: 0

Role of microglial activation on neuronal excitability in rat substantia gelatinosa 小胶质细胞活化对大鼠明胶质神经元兴奋性的影响

International Journal of Oral Biology Pub Date : 2020-12-30 DOI: 10.11620/IJOB.2020.45.4.225

A. Park, S. Chun

{"title":"Role of microglial activation on neuronal excitability in rat substantia gelatinosa","authors":"A. Park, S. Chun","doi":"10.11620/IJOB.2020.45.4.225","DOIUrl":"https://doi.org/10.11620/IJOB.2020.45.4.225","url":null,"abstract":"Glial cells, including astrocytes and microglia, interact closely with neurons and modulate pain transmission, particularly under pathological conditions. In this study, we examined the excitability of substantia gelatinosa (SG) neurons of the spinal dorsal horn using a patch clamp recording to investigate the roles of microglial activation in the nociceptive processes of rats. We used xanthine/xanthine oxidase (X/XO), a generator of superoxide anion (O2∙–), to induce a pathological pain condition. X/XO treatment induced an inward current and membrane depolarization. The inward current was significantly inhibited by minocycline, a microglial inhibitor, and fluorocitrate, an astrocyte inhibitor. To examine whether toll-like receptor 4 (TLR4) in microglia was involved in the inward current, we used lipopolysaccharide (LPS), a highly specific TLR4 agonist. The LPS induced inward current, which was decreased by pretreatment with Tak-242, a TLR4-specific inhibitor, and phenyl N-t-butylnitrone, a reactive oxygen species scavenger. The X/XO-induced inward current was also inhibited by pretreatment with Tak-242. These results indicate that the X/XO-induced inward current of SG neurons occurs through activation of TLR4 in microglial cells, suggesting that neuroglial cells modulate the nociceptive process through central sensitization.","PeriodicalId":14180,"journal":{"name":"International Journal of Oral Biology","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85678481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green tea polyphenol (–)-epigallocatechin-3-gallate prevents ultraviolet-induced apoptosis in PC12 cells 绿茶多酚(-)-表没食子儿茶素-3-没食子酸酯防止紫外线诱导的PC12细胞凋亡

International Journal of Oral Biology Pub Date : 2020-12-30 DOI: 10.11620/IJOB.2020.45.4.179

S. Woo, Kim Yoon-Jung, Bangrong Cai, S. Park, Young Ho Kim, Ok-Joon Kim, I. Kang, Won-Jae Kim, Ji-Yeon Jung

{"title":"Green tea polyphenol (–)-epigallocatechin-3-gallate prevents ultraviolet-induced apoptosis in PC12 cells","authors":"S. Woo, Kim Yoon-Jung, Bangrong Cai, S. Park, Young Ho Kim, Ok-Joon Kim, I. Kang, Won-Jae Kim, Ji-Yeon Jung","doi":"10.11620/IJOB.2020.45.4.179","DOIUrl":"https://doi.org/10.11620/IJOB.2020.45.4.179","url":null,"abstract":"Green tea polyphenol (–)-epigallocatechin-3-gallate (EGCG) is a potent antioxidant with protective effects against neurotoxicity. However, it is currently unclear whether EGCG protects neuronal cells against radiation-induced damage. Therefore, the objective of this study was to investigate the effects of EGCG on ultraviolet (UV)-induced oxidative stress and apoptosis in PC12 cells. The effects of UV irradiation included apoptotic cell death, which was associated with DNA fragmentation, reactive oxygen species (ROS) production, enhanced caspase-3 and caspase-9 activity, and poly (ADP-ribose) polymerase cleavage. UV irradiation also increased the Bax/Bcl-2 ratio and mitochondrial pathway-associated cytochrome c expression. However, pretreatment with EGCG before UV exposure markedly decreased UV-induced DNA fragmentation and ROS production. Furthermore, the UV irradiationinduced increase in Bax/Bcl-2 ratio, cytochrome c upregulation, and caspase-3 and caspase-9 activation were each ameliorated by EGCG pretreatment. Additionally, EGCG suppressed UV-induced phosphorylation of p38 and rescued UV-downregulated phosphorylation of ERK. Taken together, these results suggest that EGCG prevents UV irradiationinduced apoptosis in PC12 cells by scavenging ROS and inhibiting the mitochondrial pathways known to play a crucial role in apoptosis. In addition, EGCG inhibits UV-induced apoptosis via JNK inactivation and ERK activation in PC12 cells. Thus, EGCG represents a potential neuroprotective agent that could be applied to prevent neuronal cell death induced by UV irradiation.","PeriodicalId":14180,"journal":{"name":"International Journal of Oral Biology","volume":"67 1","pages":"179-189"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86894269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increased SOX2 expression in three-dimensional sphere culture of dental pulp stem cells 牙髓干细胞三维球培养中SOX2表达增加

International Journal of Oral Biology Pub Date : 2020-12-30 DOI: 10.11620/IJOB.2020.45.4.197

Eun Jin Seo, I. Jang

{"title":"Increased SOX2 expression in three-dimensional sphere culture of dental pulp stem cells","authors":"Eun Jin Seo, I. Jang","doi":"10.11620/IJOB.2020.45.4.197","DOIUrl":"https://doi.org/10.11620/IJOB.2020.45.4.197","url":null,"abstract":"Mesenchymal stem cells in the dental pulp exhibit a tendency for differentiation into various dental lineages and hold great potential as a major conduit for regenerative treatment in dentistry. Although they can be readily isolated from teeth, the exact characteristics of these stem cells have not been fully understood so far. When compared to twodimensional (2D) cultures, three-dimensional (3D) cultures have the advantage of enriching the stem cell population. Hence, 3D-organoid culture and 3D-sphere culture were applied to dental pulp cells in the current study. Although the establishment of the organoid culture proved unsuccessful, the 3D-sphere culture readily initiated the stable generation of cell aggregates, which continued to grow and could be passaged to the second round. Interestingly, a significant increase in SOX2 expression was detected in the 3D-spheroid culture compared to the 2D culture. These results indicate the enrichment of the stemness-high population in the 3D-sphere culture. Thus, 3D-sphere culture may act as a link between the conventional and 3D-organoid cultures and aid in understanding the characteristics of dental pulp stem cells.","PeriodicalId":14180,"journal":{"name":"International Journal of Oral Biology","volume":"19 1","pages":"197-203"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84496061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0