International Journal of Oral Biology最新文献_第6页

L-ascorbic acid induces apoptosis in human laryngeal epidermoid Hep-2 cells by modulating the nuclear factor kappa-light-chain-enhancer of activated B cells/ mitogen-activated protein kinase/Akt signaling pathway l -抗坏血酸通过调节活化B细胞/丝裂原活化蛋白激酶/Akt信号通路的核因子kappa-轻链增强子诱导人喉表皮样Hep-2细胞凋亡

International Journal of Oral Biology Pub Date : 2020-12-30 DOI: 10.11620/IJOB.2020.45.4.169

J. Park, Yoon-Jung Kim, S. Park, Kyung-Yi Chung, Sang-Jin Oh, Won-Jae Kim, Ji-Yeon Jung

{"title":"L-ascorbic acid induces apoptosis in human laryngeal epidermoid Hep-2 cells by modulating the nuclear factor kappa-light-chain-enhancer of activated B cells/ mitogen-activated protein kinase/Akt signaling pathway","authors":"J. Park, Yoon-Jung Kim, S. Park, Kyung-Yi Chung, Sang-Jin Oh, Won-Jae Kim, Ji-Yeon Jung","doi":"10.11620/IJOB.2020.45.4.169","DOIUrl":"https://doi.org/10.11620/IJOB.2020.45.4.169","url":null,"abstract":"L-ascorbic acid (L-AA; vitamin C) induces apoptosis in cancer cells. This study aimed to elucidate the molecular mechanisms of L-AA-induced apoptosis in human laryngeal epidermoid carcinoma Hep-2 cells. L-AA suppressed the viability of Hep-2 cells and induced apoptosis, as shown by the cleavage and condensation of nuclear chromatin and increased number of Annexin V-positive cells. L-AA decreased Bcl-2 protein expression but upregulated Bax protein levels. In addition, cytochrome c release from the mitochondria into the cytosol and activation of caspase-9, -8, and -3 were enhanced by L-AA treatment. Furthermore, apoptosis-inducing factor (AIF) and endonuclease G (EndoG) were translocated into the nucleus during apoptosis of L-AA-treated Hep-2 cells. L-AA effectively inhibited the constitutive nuclear factor-κB (NF-κB) activation and attenuated the nuclear expression of the p65 subunit of NF-κB. Interestingly, L-AA treatment of Hep-2 cells markedly activated Akt and mitogen-activated protein kinase (MAPK; extracellular signal-regulated kinase 1/2, p38, and c-Jun N-terminal kinase [JNK]) and and LY294002 (Akt inhibitor), SB203580 (p38 inhibitor) or SP600125 (a JNK inhibitor) decreased the levels of Annexin V-positive cells. These results suggested that L-AA induces the apoptosis of Hep-2 cells via the nuclear translocation of AIF and EndoG by modulating the Bcl- 2 family and MAPK/Akt signaling pathways.","PeriodicalId":14180,"journal":{"name":"International Journal of Oral Biology","volume":"82 1","pages":"169-178"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88101945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High concentration of calcium represses osteoblast differentiation in C2C12 cells 高浓度钙抑制C2C12细胞成骨细胞分化

International Journal of Oral Biology Pub Date : 2020-12-30 DOI: 10.11620/IJOB.2020.45.4.162

Y. J. Lee, Younho Han

{"title":"High concentration of calcium represses osteoblast differentiation in C2C12 cells","authors":"Y. J. Lee, Younho Han","doi":"10.11620/IJOB.2020.45.4.162","DOIUrl":"https://doi.org/10.11620/IJOB.2020.45.4.162","url":null,"abstract":"Calcium is the most abundant stored mineral in the human body and is especially vital for bone health; thus, calcium deficiency can cause bone-related diseases, such as osteopenia and osteoporosis. However, a high concentration of serum calcium, which is commonly known as hypercalcemia, can also lead to weakened bones and, in severe cases, osteosarcoma. Therefore, it is necessary to maintain the concentration of calcium that is appropriate for bone biology. In the present study, we aimed to elucidate the effects of high concentration of calcium, approximately 2 folds the normal calcium level, on osteoblast differentiation. The CaCl2 treatment showed dose-dependent suppression of the alkaline phosphatase activity and mineralized nodule formation. Calcium showed cytotoxicity at an extremely high concentration, but a moderately high concentration of calcium that results in inhibitory effects to osteoblast differentiation showed no signs of cytotoxicity. We also confirmed that the CaCl2 treatment repressed the mRNA expression and protein abundance of various osteogenic genes and transcriptional factors. Considered together, these results indicate that a high concentration of calcium negatively regulates the osteoblast differentiation of C2C12 cells.","PeriodicalId":14180,"journal":{"name":"International Journal of Oral Biology","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78706786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-salt and glucose diet attenuates alveolar bone recovery in a ligature-induced rat model of experimental periodontitis 在结扎诱导的实验性牙周炎大鼠模型中，高盐和高糖饮食减缓了牙槽骨的恢复

International Journal of Oral Biology Pub Date : 2020-12-30 DOI: 10.11620/IJOB.2020.45.4.204

Wan-Ruoh Lee, Seoung Hoon Lee, Min-Seuk Kim, E. Choi

{"title":"High-salt and glucose diet attenuates alveolar bone recovery in a ligature-induced rat model of experimental periodontitis","authors":"Wan-Ruoh Lee, Seoung Hoon Lee, Min-Seuk Kim, E. Choi","doi":"10.11620/IJOB.2020.45.4.204","DOIUrl":"https://doi.org/10.11620/IJOB.2020.45.4.204","url":null,"abstract":"Excessive intake of sodium caused by high salt diet promotes the expression of inflammatory cytokines and differentiation of helper T cells resulting in inflammatory responses. High-glucose diet also contributes to the pathogenesis of periodontitis by inducing changes in the oral microbiome and reducing salivation. However, the effect of a high-salt and glucose diet (HSGD) on the prognosis of periodontitis remains unclear. In this study, a rat model of experimental periodontitis was established by periodic insertion of absorbable sutures containing Porphyromonas gingivalis and Fusobacterium nucleatum strains into the right gingival sulcus to analyze the effect of HSGD on the incidence and progression of periodontitis. The alveolar bone heights (ABH) was measured with microcomputed tomography imaging of the HSGD- and general diet (GD)-treated groups. The right ABH was significantly decreased compared to the left in both groups at 4 weeks after induction of inflammation; however, no significant difference was noted between the groups. Notably, the ABH in the HSGD-treated group was significantly decreased at 8 weeks after induction of inflammation, whereas in the GD-treated group, an increase in the ABH was observed; a significant difference of the ABH was noted between the two groups (p < 0.05). At 12 weeks, recovery of the alveolar bone was observed in both groups, with no significant differences in ABH between the two groups. These findings indicate that the intake of excessive sodium attenuates the recovery rate of the alveolar bone even after the local infectant is removed. In addition, this study demonstrates the use of HSGD in establishing a new animal model of periodontitis.","PeriodicalId":14180,"journal":{"name":"International Journal of Oral Biology","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82905651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Next-generation sequencing analysis of exosomal microRNAs: Fusobacteriumnucleatum regulates the expression profiling of exosomal microRNAs in human colorectal cancer cells 外泌体microRNAs的下一代测序分析:核梭杆菌调节人类结直肠癌细胞外泌体microRNAs的表达谱

International Journal of Oral Biology Pub Date : 2020-09-30 DOI: 10.11620/ijob.2020.45.3.134

M. Yu, Hye Jung Kim, J. Kang, Y. Kim, H. Park

{"title":"Next-generation sequencing analysis of exosomal microRNAs: Fusobacteriumnucleatum regulates the expression profiling of exosomal microRNAs in human colorectal cancer cells","authors":"M. Yu, Hye Jung Kim, J. Kang, Y. Kim, H. Park","doi":"10.11620/ijob.2020.45.3.134","DOIUrl":"https://doi.org/10.11620/ijob.2020.45.3.134","url":null,"abstract":"Colon cancer is one of the most common malignant tumors, but there are still a few validated biomarkers of colon cancer. Exosome-mediated microRNAs (miRNAs) have been recognized as potential biomarkers in cancers, and miRNAs can regulate a variety of genes. Recently, Fusobacterium nucleatum was discovered in the tissues of human colon cancer patients. Its role in colon cancer was highlighted. F. nucleatum may contribute to the progression of colon cancer through the mechanism of exosome-mediated miRNAs transfer. However, the exosomal miRNAs regulation mechanism by F. nucleatum in colon cancer is not well known. Thus, we performed next-generation sequencing to investigate the overall pattern of exosomal miRNAs expression in the colon cancer cell culture supernatant. We have confirmed the alterations of various exosomal miRNAs. In addition, to investigate the function of exosomal miRNAs, a Kyoto Encyclopedia of Genes and Genomes analysis was performed on the target genes of changed miRNAs. Potential target genes were associated with a variety of signaling pathways, and one of these pathways was related to colorectal cancer. These findings suggested that F. nucleatum can alter exosomal miRNAs released from colorectal cancer cells. Furthermore, exosomal miRNAs altered by F. nucleatum could be potential biomarkers for the diagnosis and therapy of colon cancer.","PeriodicalId":14180,"journal":{"name":"International Journal of Oral Biology","volume":"55 1","pages":"134-142"},"PeriodicalIF":0.0,"publicationDate":"2020-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87914892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Participation of D-serine and NR2 subunits in EphA4-mediated trigeminal neuropathic pain d -丝氨酸和NR2亚基参与epha4介导的三叉神经性疼痛

International Journal of Oral Biology Pub Date : 2020-09-30 DOI: 10.11620/ijob.2020.45.3.84

M. Kim, Min-Ji Kim, Jo-Young Son, Yu-Mi Kim, J. Ju, D. Ahn

{"title":"Participation of D-serine and NR2 subunits in EphA4-mediated trigeminal neuropathic pain","authors":"M. Kim, Min-Ji Kim, Jo-Young Son, Yu-Mi Kim, J. Ju, D. Ahn","doi":"10.11620/ijob.2020.45.3.84","DOIUrl":"https://doi.org/10.11620/ijob.2020.45.3.84","url":null,"abstract":"The present study investigated the participation of D-serine and NR2 in antinociception produced by blockade of central erythropoietin-producing hepatocellular carcinoma (Eph) A4 (EphA4) signaling in rats with trigeminal neuropathic pain. Trigeminal neuropathic pain was modeled in male Sprague-Dawley rats using mal-positioned dental implants. The left mandibular second molar was extracted under anesthesia, and a miniature dental implant was placed to induce injury to the inferior alveolar nerve. Our current findings showed that nerve injury induced by malpositioned dental implants significantly produced mechanical allodynia; additionally, the inferior alveolar nerve injury increased the expression of D-serine and NR2 subunits in the ipsilateral medullary dorsal horn (trigeminal subnucleus caudalis). Intracisternal administration of EphA4-Fc, an EphA4 inhibitor, inhibited nerve injury-induced mechanical allodynia and upregulated the expression of D-serine and NR2 subunits. Moreover, intracisternal administration of D-amino acids oxidase, a D-serine inhibitor, inhibited trigeminal mechanical allodynia. These results show that D-serine and NR2 subunit pathways participate in central EphA4 signaling after an inferior alveolar nerve injury. Therefore, blockade of D-serine and NR2 subunit pathways in central EphA4 signaling provides a new therapeutic target for the treatment of trigeminal neuropathic pain.","PeriodicalId":14180,"journal":{"name":"International Journal of Oral Biology","volume":"40 1","pages":"84-91"},"PeriodicalIF":0.0,"publicationDate":"2020-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88481906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deficiencies of Homer2 and Homer3 accelerate aging-dependent bone loss in mice 缺乏Homer2和Homer3会加速小鼠衰老依赖性骨质流失

International Journal of Oral Biology Pub Date : 2020-09-30 DOI: 10.11620/ijob.2020.45.3.126

J. Y. Kang, Namju Kang, D. Shin, Yu-Mi Yang

{"title":"Deficiencies of Homer2 and Homer3 accelerate aging-dependent bone loss in mice","authors":"J. Y. Kang, Namju Kang, D. Shin, Yu-Mi Yang","doi":"10.11620/ijob.2020.45.3.126","DOIUrl":"https://doi.org/10.11620/ijob.2020.45.3.126","url":null,"abstract":"Homer proteins are scaffold proteins that regulate calcium (Ca2+) signaling by modulating the activity of multiple Ca2+ signaling proteins. In our previous report, Homer2 and Homer3 regulated NFATc1 function through its interaction with calcineurin, which then acted to regulate receptor activator of nuclear factor-kappa B ligand (RANKL)-induced osteoclastogenesis and bone metabolism. However, to date, the role of Homers in osteoclastogenesis remains unknown. In this study, we investigated the roles of Homer2 and Homer3 in aging-dependent bone remodeling. Deletion of Homer2 /Homer3 (Homer2/3 DKO) markedly decreased the bone density of the femur. The decrease in bone density was not seen in mice with Homer2 (Homer2−/−) and Homer3 (Homer3−/−) deletion. Moreover, RANKL treatment of bone marrow-derived monocytes/macrophages in Homer2/3 DKO mice significantly increased the formation of multinucleated cells and resorption areas. Finally, Homer2/3 DKO mice decreased bone density in an aging-dependent manner. These findings suggest a novel potent mode of bone homeostasis regulation through osteoclasts differentiation during aging by Homer proteins, specifically Homer2 and Homer3.","PeriodicalId":14180,"journal":{"name":"International Journal of Oral Biology","volume":"10 5","pages":"126-133"},"PeriodicalIF":0.0,"publicationDate":"2020-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72607165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Practical considerations for the study of the oral microbiome 口腔微生物组研究的实际考虑

International Journal of Oral Biology Pub Date : 2020-09-30 DOI: 10.11620/ijob.2020.45.3.77

Yeuni Yu, Seo-young Lee, H. Na

引用次数: 0

Inhibition of cell growth and induction of apoptosis by acacetin in FaDu human pharyngeal carcinoma cells 阿卡乙素对FaDu人咽癌细胞生长的抑制及诱导凋亡的作用

International Journal of Oral Biology Pub Date : 2020-09-30 DOI: 10.11620/ijob.2020.45.3.107

Kang Kyeong-Rok, K. jae-sung, Kim Tae-Hyeon, Seo Jeong-Yeon, Park Jong-Hyun, Lim Jin-Woong, Yu Sun-Kyoung, Kim Heung-Joong, Shin Sang-Hun, Park, Bo-Ram, Kim Chun-Sung, Kim Do-Kyung

{"title":"Inhibition of cell growth and induction of apoptosis by acacetin in FaDu human pharyngeal carcinoma cells","authors":"Kang Kyeong-Rok, K. jae-sung, Kim Tae-Hyeon, Seo Jeong-Yeon, Park Jong-Hyun, Lim Jin-Woong, Yu Sun-Kyoung, Kim Heung-Joong, Shin Sang-Hun, Park, Bo-Ram, Kim Chun-Sung, Kim Do-Kyung","doi":"10.11620/ijob.2020.45.3.107","DOIUrl":"https://doi.org/10.11620/ijob.2020.45.3.107","url":null,"abstract":"Acacetin, which is present in damiana (Turnera diffusa ) and black locust (Robinia pseudoacacia ), has several pharmacologic activities such as antioxidant, anti-inflammatory, and anti-proliferative effects on cancer cells. However, the effect of acacetin on head and neck cancers has not been clearly established. This study aimed to examine the effects of acacetin on cell growth and apoptosis induction in FaDu human pharyngeal carcinoma cells. These were investigated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay, Live/Dead cell assay, 4′,6-diamidino-2-phenylindole dihydrochloride staining, caspase-3 and caspase-7 activation assay, and immunoblotting in FaDu cells. Acacetin induced FaDu cell death in a dose-dependent manner, with an estimated IC50 value of 41.9 µM, without affecting the viability of L-929 mouse fibroblasts as normal cells. Acacetin treatment resulted in nuclear condensation in the FaDu cells. It promoted the proteolytic cleavage of procaspase-3, -7, -8, and -9 with increasing amounts of the cleaved caspase isoforms in FaDu cells. Acacetin-induced apoptosis in FaDu cells was mediated by the expression of Fas and activation of caspase-8, caspase-3, and poly (ADP-ribose) polymerase. Immunoblotting showed downregulation of the anti-apoptotic mitochondrial proteins Bcl-2 and Bcl-xL, but upregulation of the mitochondria-dependent pro-apoptotic proteins Bax and Badin FaDu cells after acacetin treatment. These findings indicate that acacetin inhibits cell proliferation and induces apoptotic cell death in FaDu human pharyngeal carcinoma cells via both the death receptor-mediated extrinsic apoptotic pathway and the mitochondria-mediated intrinsic apoptotic pathway.","PeriodicalId":14180,"journal":{"name":"International Journal of Oral Biology","volume":"38 1","pages":"107-114"},"PeriodicalIF":0.0,"publicationDate":"2020-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74195764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phagocytic osteoclasts in the alveolar bone of diabetic rats with periodontitis 糖尿病牙周炎大鼠牙槽骨吞噬破骨细胞的研究

International Journal of Oral Biology Pub Date : 2020-09-30 DOI: 10.11620/ijob.2020.45.3.92

B. Eun-Jung, Kim Ae-Ri, Kim, Ji-hye, Yoo Yun-Jung

{"title":"Phagocytic osteoclasts in the alveolar bone of diabetic rats with periodontitis","authors":"B. Eun-Jung, Kim Ae-Ri, Kim, Ji-hye, Yoo Yun-Jung","doi":"10.11620/ijob.2020.45.3.92","DOIUrl":"https://doi.org/10.11620/ijob.2020.45.3.92","url":null,"abstract":"Periodontitis is a bacteria-induced inflammatory disease associated with alveolar bone loss. Osteoclast is a macrophage-lineage cell that exhibits phagocytic activity; however, osteoclast phagocytic activity has not been demonstrated under pathological conditions. Diabetes is a pathological condition that exacerbates alveolar bone loss via periodontitis; therefore, we examined phagocytic osteoclasts in diabetic rats that had periodontitis. The rats were divided into the control (C), periodontitis (P), and diabetes with periodontitis (DP) groups. Diabetes and periodontitis were induced by streptozotocin injection and ligature of the mandibular first molars, respectively. On days 3 and 20 after the ligature, the rats were sacrificed, and osteoclasts containing inclusions were quantified by tartrate-resistant acid phosphatase staining. On day 3, there were more osteoclasts containing inclusions in the DP group than in the C group. Among inclusions, osteocyte-like cells and dense bodies were more frequently observed in the DP group than in the C group. Cytoplasm-like structures were elevated more in the DP group than in the C and P groups. However, no differences were observed on day 20. Interestingly, some osteoclasts were in contact with the osteocytes within the exposed lacunae and contained several inclusions within a large vacuole. Thus, the elevation of phagocytic osteoclasts in rats with diabetes and periodontitis provides insight into the role of osteoclast phagocytic activity under pathological conditions.","PeriodicalId":14180,"journal":{"name":"International Journal of Oral Biology","volume":"52 1","pages":"92-98"},"PeriodicalIF":0.0,"publicationDate":"2020-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90746763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resveratrol inhibits cell growth via targeting the Bmi-1 pathway in YD-10B human oral squamous cell carcinoma cells 白藜芦醇通过靶向pd - 10b人口腔鳞状细胞癌细胞的Bmi-1通路抑制细胞生长

International Journal of Oral Biology Pub Date : 2020-09-30 DOI: 10.11620/ijob.2020.45.3.115

Kyoung‐Eun Park, C. Y. Ok, H. Jang, M. Bae, S. Bae

{"title":"Resveratrol inhibits cell growth via targeting the Bmi-1 pathway in YD-10B human oral squamous cell carcinoma cells","authors":"Kyoung‐Eun Park, C. Y. Ok, H. Jang, M. Bae, S. Bae","doi":"10.11620/ijob.2020.45.3.115","DOIUrl":"https://doi.org/10.11620/ijob.2020.45.3.115","url":null,"abstract":"Resveratrol has been reported to exert anticancer activity via modulation of multiple pathways and genes. In this study, we examined the effect of resveratrol on YD-10B human oral squamous cell carcinoma cells and its molecular mechanisms of action. We found that resveratrol inhibited the proliferation of YD-10B cells in a dose- and timedependent manner. The suppressive effect of resveratrol was accompanied by a reduction in Bmi-1 gene expression. We observed that silencing the Bmi-1 gene by small interfering RNA effectively downregulated the levels of GLUT1 mRNA and protein, which were also repressed by resveratrol. Bmi-1 silencing increased the number of YD-10B cells in S-phase arrest by approximately 2.3-fold compared with the control. In conclusion, the results of the present study demonstrate, for the first time, that resveratrol suppresses Bmi-1-mediated GLUT1 expression in human oral squamous cell carcinoma cells and suggest that the specific molecular targeting of Bmi-1 and/or GLUT1 expression can be combined with a chemotherapeutic strategy to improve the response of oral cancer cells to resveratrol.","PeriodicalId":14180,"journal":{"name":"International Journal of Oral Biology","volume":"34 1","pages":"115-125"},"PeriodicalIF":0.0,"publicationDate":"2020-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90829414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0