International journal of industrial entomology最新文献_第8页

Simultaneous detection of fungal, bacterial, and viral pathogens in insects by multiplex PCR and capillary electrophoresis 多重PCR和毛细管电泳同时检测昆虫真菌、细菌和病毒病原体

International journal of industrial entomology Pub Date : 2015-06-30 DOI: 10.7852/IJIE.2015.30.2.64

Kyu‐Won Kwak, S. Nam, Ji-Young Choi, Seokhyung Lee, H. Kim, Sung Hyun Kim, K. Park, Myung‐Sae Han

{"title":"Simultaneous detection of fungal, bacterial, and viral pathogens in insects by multiplex PCR and capillary electrophoresis","authors":"Kyu‐Won Kwak, S. Nam, Ji-Young Choi, Seokhyung Lee, H. Kim, Sung Hyun Kim, K. Park, Myung‐Sae Han","doi":"10.7852/IJIE.2015.30.2.64","DOIUrl":"https://doi.org/10.7852/IJIE.2015.30.2.64","url":null,"abstract":"Beetles Protaetia brevitarsis seulensis Kolbe (Coleoptera: Cetoniidae) and Allomyrina dichotoma Linn. (Coleoptera: Scarabaeidae) are widely used in traditional medicine, and the number of insect-rearing farms is increasing in South Korea. The purpose of this study was to establish a multiplex PCR-based assay for rapid simultaneous detection of multiple pathogens causing insect diseases. Six insect parasites such as fungi Beauveria bassiana (Bals.-Criv.) Vuill. (Hypocreales: Cordycipitaceae) and Metarhizium anisopliae (Metschn.) Sorokin (Hypocreales: Clavicipitaceae), bacteria Bacillus thuringiensis Berliner (Bacillales: Bacillaceae), Pseudomonas aeruginosa Migula (Pseudomonadales: Pseudomonadaceae), and Serratia marcescens Bizio (Enterobacteriales: Enterobacteriaceae), and Oryctes rhinoceros nudivirus were chosen based on the severity and incidence rate of insect diseases in South Korea. Pathogen-specific primers were designed and successfully applied for simultaneous detection of multiple infectious agents in farm-bred insects P. b. seulensis and A. dichotoma using multiplex PCR and high resolution capillary electrophoresis. Our results indicate that multiplex PCR is an effective and time-saving method for simultaneous detection of multiple infections in insects, and the QIAxcel capillary electrophoresis system is useful for quantitative evaluation of the individual impact of each infectious agent on the severity of insect disease. The approach designed in this study can be utilized for rapid and accurate diagnostics of infection in insect farms.","PeriodicalId":14140,"journal":{"name":"International journal of industrial entomology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79541841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Expression of porcine circovirus type 2 capsid protein fused with partial polyhedrin using baculovirus 猪圆环病毒2型衣壳蛋白与部分多面蛋白融合的杆状病毒表达

International journal of industrial entomology Pub Date : 2015-06-30 DOI: 10.7852/IJIE.2015.30.2.50

Jun Beom Lee, S. Bae, T. Shin, S. Woo

{"title":"Expression of porcine circovirus type 2 capsid protein fused with partial polyhedrin using baculovirus","authors":"Jun Beom Lee, S. Bae, T. Shin, S. Woo","doi":"10.7852/IJIE.2015.30.2.50","DOIUrl":"https://doi.org/10.7852/IJIE.2015.30.2.50","url":null,"abstract":"Porcine circovirus type 2 (PCV2) is an important infectious swine virus causing postweaning multisystemic wasting syndrome (PMWS). PCV2 capsid protein, encoded by ORF2 has type- specific epitopes, is very immunogenic, and is associated with the induction of neutralizing antibodies. For the efficient production of capsid protein, recombinant Autographa californica nucleopolyhedroviruses were generated to express ORF2 fused with two forms of a partial polyhedrin. Recombinant capsid protein was produced successfully with the partial polyhedrin fusion form and the yield was high, as was shown by SDS-PAGE. Production of recombinant capsid proteins in insect cells was confirmed by Western blot analysis using anti- His monoclonal antibody, anti-ORF2 monoclonal antibody, and anti-PCV2 porcine serum. Fusion expression with amino acids 19 to 110 of the polyhedrin increased the production of recombinant capsid protein, but fusion with amino acids 32 to 85 did not. Additionally, PCV2 capsid protein is a glycoprotein; however, the glycosylation of recombinant protein was not observed. The results of an Enzyme-linked immunosorbent assay (ELISA) showed that recombinant capsid proteins could be utilized as antigens for fast, large-scale diagnosis of PCV2-infected pigs. Our results suggest that the fusion expression of partial polyhedrin is able to increase the production of recombinant PCV2 capsid protein in insect cells.","PeriodicalId":14140,"journal":{"name":"International journal of industrial entomology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80318845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Silkworm-food plant-interaction: search for an alternate food plant for tasar silkworm (Antheraea mylitta Drury) rearing 蚕-食性植物-相互作用:寻找一种可替代的沙蚕(Antheraea mylitta Drury)饲养的食性植物

International journal of industrial entomology Pub Date : 2015-06-30 DOI: 10.7852/IJIE.2015.30.2.58

M. Deka, G. Gargi, Rajendra Kumar, H. Yadav, A. Sahay

{"title":"Silkworm-food plant-interaction: search for an alternate food plant for tasar silkworm (Antheraea mylitta Drury) rearing","authors":"M. Deka, G. Gargi, Rajendra Kumar, H. Yadav, A. Sahay","doi":"10.7852/IJIE.2015.30.2.58","DOIUrl":"https://doi.org/10.7852/IJIE.2015.30.2.58","url":null,"abstract":"An experimental rearing of tropical tasar silkworm, Antheraea mylitta Drury was conducted to study silkworm-food plant-interaction and thereby to search for an alternate silkworm food plant. The silkworm-food-plant-interaction was studied with six different food plant species viz. Terminalia tomentosa, Terminalia arjuna, Terminalia belerica, Terminalia chebula of Combretaceae family and Lagerstroemia speciosa, Lagerstroemia parviflora of Lythraceae family. The rearing performance of silkworm on Lagerstroemia speciosa in terms of cocoons per DFL and silk ratio was found comparable with Terminalia tomentosa and Termonalia arjuna, the primary tasar silkworm food plant species. These three plant species also possessed better results in terms of physiological (leaf moisture content and net photosynthesis rate) and biochemical (Chlorophyll, protein, carbohydrate and crude fibre contents) characteristics to support silkworm rearing than Terminalia belerica, Terminalia chebula and Lagerstroemia parviflora. The correlation study between silkworm rearing performance and food plant`s constituents indicates commercial perspective of Lagerstroemia speciosa as an alternate food plant for tasar silkworm rearing.","PeriodicalId":14140,"journal":{"name":"International journal of industrial entomology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76643214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effect of different Bombyx mori silkworm varieties on the wet spinning of silk fibroin 不同家蚕品种对湿法纺丝丝素蛋白的影响

International journal of industrial entomology Pub Date : 2015-06-30 DOI: 10.7852/IJIE.2015.30.2.75

M. Jang, I. Um

引用次数: 2

Developmental characteristics of Zophobas atratus (Coleoptera: Tenebrionidae) larvae in different instars 白蛉(鞘翅目:拟甲科)不同龄期幼虫的发育特征

International journal of industrial entomology Pub Date : 2015-06-30 DOI: 10.7852/IJIE.2015.30.2.45

Sunyoung Kim, H. Kim, S. Song, N. Kim

{"title":"Developmental characteristics of Zophobas atratus (Coleoptera: Tenebrionidae) larvae in different instars","authors":"Sunyoung Kim, H. Kim, S. Song, N. Kim","doi":"10.7852/IJIE.2015.30.2.45","DOIUrl":"https://doi.org/10.7852/IJIE.2015.30.2.45","url":null,"abstract":"The giant mealworm beetle, Zophobas atratus (Coleoptera: Tenebrionidae), is reared for commercial purposes as a live feed for animals. In 2011, it was officially introduced in Korea, and since then it has been considered commercially important. This beetle is a good animal feed resource because of its high protein content with balanced nutrients. However, its life stage characteristics have not been clearly described, especially of the larval stage that can be used as commercial products. To this end, in our study, we determined the number of Z. atratus larval instars, and described their characteristics at each stage, providing basic information about this beetle. Z. atratus larvae required eight to nine d of incubation period before hatching. The first instar period comprised three to four d. There were relatively large variations in each instar period, except for the first instar. Before the adults emerged, most of the individuals passed through15 to 18 instars. The highest pupation rate, 25.71%, was observed in both 16th and 17th instars. Body length gradually increased with each successive instar and it reached its maximum at the 18th instar. The color of larvae was white at the first instar, and gradually turned brown after the second instar.","PeriodicalId":14140,"journal":{"name":"International journal of industrial entomology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83298457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Structure and properties of silk sericin obtained from different silkworm varieties 不同家蚕品种丝胶蛋白的结构和性质

International journal of industrial entomology Pub Date : 2015-06-30 DOI: 10.7852/IJIE.2015.30.2.81

D. Chung, Ji Hye Lee, H. Kweon, Kwang-gill Lee, I. Um

引用次数: 8

Effects of silk fibroin hydrolysate on bone metabolism in ovariectomized rats 丝素蛋白水解物对去卵巢大鼠骨代谢的影响

International journal of industrial entomology Pub Date : 2015-03-31 DOI: 10.7852/IJIE.2015.30.1.17

H. Kweon, S. Shin, J. Chon, Kwang-gill Lee, You-Young Jo, J. Yoon, Y. Park, J. Jeon, Jong-Ho Kim, Bong-Seob Shin

{"title":"Effects of silk fibroin hydrolysate on bone metabolism in ovariectomized rats","authors":"H. Kweon, S. Shin, J. Chon, Kwang-gill Lee, You-Young Jo, J. Yoon, Y. Park, J. Jeon, Jong-Ho Kim, Bong-Seob Shin","doi":"10.7852/IJIE.2015.30.1.17","DOIUrl":"https://doi.org/10.7852/IJIE.2015.30.1.17","url":null,"abstract":"This study aimed to investigate the effects of silk fibroin on bone metabolism in ovariectomized rats. A total of 30 Sprague-Dawley rats were randomized into sham-operated (SHAM), ovariectomized control (OVX), alendronate (OVX+ALEN, 10 mg/kg body weight/d), low silk fibroin (OVX+SF100, 100 mg/kg body weight/d), and high silk fibroin (OVX+SF300, 300 mg/kg body weight/d) groups. All the rats were fed by gavage for 12 wk. At the end of 12 wk, blood and urine were collected for analysis of bone turnover markers, and bone mineral density (BMD) was measured by micro-computed tomography. The results show that the OVX group (p < 0.05) displayed the highest mean body weight gain. Among the five groups, serum levels of bone alkaline phosphatase (ALP) and urine levels of deoxypyridinoline (DPD) were highest in the OVX group (p < 0.05). Bone ALP levels in the ALEN group were significantly lower than that of the silk-treated groups. On the other hand, DPD levels were not significantly different between the ALEN and silk-fibroin-treated groups ( p < 0.05). The trabecular BMD was significantly higher in the ALEN and silk-treated groups compared to the OVX group ( p < 0.05). In conclusion, this study showed that silk fibroin has similar effects as alendronate, which is used in osteoporosis medication. Therefore silk fibroin might be a new candidate for the prevention and treatment of osteoporosis in patients.","PeriodicalId":14140,"journal":{"name":"International journal of industrial entomology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82492825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Monitoring of phase separation between silk fibroin and sericin using various dye system 不同染料体系下丝素与丝胶相分离的监测

International journal of industrial entomology Pub Date : 2015-03-31 DOI: 10.7852/IJIE.2015.30.1.1

H. Kwak, K. Lee

引用次数: 3

Biology of a predatory bug Eocanthecona furcellata Wolff (Hemiptera : Pentatomidae) on Vapourer tussock moth larvae: a major pest of tasar silkworm food plants 一种掠食性昆虫(半翅目:五蝽科)对塔沙蚕食性植物主要害虫——塔沙蚕蛾幼虫的生物学研究

International journal of industrial entomology Pub Date : 2015-03-31 DOI: 10.7852/IJIE.2015.30.1.26

Aruna Ambadahalli Siddaiah, A. Shanmuga Sundaram Devi

{"title":"Biology of a predatory bug Eocanthecona furcellata Wolff (Hemiptera : Pentatomidae) on Vapourer tussock moth larvae: a major pest of tasar silkworm food plants","authors":"Aruna Ambadahalli Siddaiah, A. Shanmuga Sundaram Devi","doi":"10.7852/IJIE.2015.30.1.26","DOIUrl":"https://doi.org/10.7852/IJIE.2015.30.1.26","url":null,"abstract":"Antheraea is polyphagous in A large number of pests are reported to both the host plant as well as silkworm. As the rearing is conducted outdoor silkworms are exposed to attack of various pests during their life cycle. Eocanthecona furcellata (Hemiptera: Pentatomidae) is the major predator of tasar silkworm during young age rearing. Both nymphs and adults of the stink bug suck the heamolymph of silkworm leading to death. The present work was undertaken to study the life cycle of E. furcellata on larvae of vapourer tussock moth which is a major pest of tasar host plants. The incubation period, nymphal duration and total developmental period from egg to adult was 8.0 ± 0.45 d, 16.0 ± 3.24 and 22.0 ± 4.20 d, respectively. Pre-oviposition, oviposition and longevity of male and female stink bug was 3.20 ± 0.38, 17.60 ± 1.86, 42.40 ± 1.94, 37.00 ± 3.18 d, respectively. Analysis of the data revealed no significant difference in life cycle of the predator when provided silkworm and vapourer tussock moth larvae as hosts. Which clearly indicates that E. furcellata can multiply on vapourer tussock moth larvae during non rearing periods. Adoption of recommended pest control measures for defoliators of tasar host plants during non-rearing periods will help in reducing the incidence of host plant pests and also in reducing the incidence of stink bug during rearing season. During the study it was also observed that larvae semilooper, boll headed caterpillar and nymphs of leaf hopper serve as alternate/secondary hosts to E. furcellata during non rearing seasons. on vapourer tussock moth larvae.","PeriodicalId":14140,"journal":{"name":"International journal of industrial entomology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91059485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Genetic characterization of microsporidians infecting Indian non-mulberry silkworms (Antheraea assamensis and Samia cynthia ricini) by using PCR based ISSR and RAPD markers assay. 基于ISSR和RAPD标记的印度非桑蚕(Antheraea assamensis和Samia cynthia ricini)微孢子虫遗传分析

International journal of industrial entomology Pub Date : 2015-03-31 DOI: 10.7852/IJIE.2015.30.1.6

W. Hassan, B. S. Nath

{"title":"Genetic characterization of microsporidians infecting Indian non-mulberry silkworms (Antheraea assamensis and Samia cynthia ricini) by using PCR based ISSR and RAPD markers assay.","authors":"W. Hassan, B. S. Nath","doi":"10.7852/IJIE.2015.30.1.6","DOIUrl":"https://doi.org/10.7852/IJIE.2015.30.1.6","url":null,"abstract":"This study established the genetic characterisation of 10 microsporidian isolates infecting non-mulberry silkworms (Antheraea assamensis and Samia cynthia ricini) collected from biogeographical forest locations in the State of Assam, India, using PCR-based markers assays: inter simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD). A Nosema type species (NIK-1s_mys) was used as control for comparison. The shape of mature microsporidian spores were observed oval to elongated, measuring 3.80 to 4.90 µm in length and 2.60 to 3.05 µm in width. Fourteen ISSR primers generated reproducible profiles and yielded 178 fragments, of which 175 were polymorphic (98%), while 16 RAPD primers generated reproducible profiles with 198 amplified fragments displaying 95% of polymorphism. Estimation of genetic distance coefficients based on dice coefficients method and clustering with un-weighted pair group method using arithmetic average (UPGMA) analysis was done to unravel the genetic diversity of microsporidians infecting Indian muga and eri silkworm. The similarity coefficients varied from 0.385 to 0.941 in ISSR and 0.083 to 0.938 in RAPD data. UPGMA analysis generated dendrograms with two microsporidian groups, which appear to be different from each other. Based on Euclidean distance matrix method, 2-dimensional distribution also revealed considerable variability among different identified microsporidians. Clustering of these microsporidian isolates was in accordance with their host and biogeographic origin. Both techniques represent a useful and efficient tool for taxonomical grouping as well as for phylogenetic classification of different microsporidians in general and genotyping of these pathogens in particular.","PeriodicalId":14140,"journal":{"name":"International journal of industrial entomology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74666224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1