N. Chandrakanth, S. M. Moorthy, K. Ponnuvel, V. Sivaprasad
{"title":"Screening and classification of mulberry silkworm, Bombyx mori based on thermotolerance","authors":"N. Chandrakanth, S. M. Moorthy, K. Ponnuvel, V. Sivaprasad","doi":"10.7852/IJIE.2015.31.2.115","DOIUrl":"https://doi.org/10.7852/IJIE.2015.31.2.115","url":null,"abstract":"The tropical climate prevailing in India adversely affects temperate bivoltine silkworm rearing and causes crop loss especially during summer. Identification of high temperature tolerant bivoltine breeds by screening for thermotolerance in the silkworm, Bombyx mori (Lepidoptera: Bombycidae) is an essential prerequisite for the development of thermotolerant bivoltine breeds / hybrids. Therefore, in this study, 20 silkworm breeds were reared at different temperatures (25 ± 1°C,32 ± 1°C, 34 ± 1°C and 36 ± 1°C) for 6 h every day from 3 rd d of th instar to till spinning. Significant differences ( p < 0.01) were found among all the rearing traits over temperature. Based on pupation percentage, SK4C and BHR3 were identified as thermotolerant bivoltine breeds. Hierarchical clustering analysis based on rearing traits at tested temperatures grouped 20 silkworm breeds in four clusters which included one cluster each of susceptible and tolerant, and two clusters of moderately tolerant silkworm breeds. This suggests that clustering based on rearing data at high temperatures by using Euclidean distance can be an effective approach in classifying the silkworm breeds on their thermotolerance capacity. The identified breeds would be used for development of thermo tolerant bivoltine silkworm breeds / hybrids.","PeriodicalId":14140,"journal":{"name":"International journal of industrial entomology","volume":"19 1","pages":"115-126"},"PeriodicalIF":0.0,"publicationDate":"2015-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72994323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Degumming of Antheraea yamamai silkworm cocoon","authors":"Bong-Seob Shin, J. Jeon, Jong-Ho Kim","doi":"10.7852/IJIE.2015.31.2.127","DOIUrl":"https://doi.org/10.7852/IJIE.2015.31.2.127","url":null,"abstract":"Oak silkworm, Antheraea yamamai (A. yamamai), has been used for clothing and surgical suture and considered as biomaterial due to RGD tripeptide. This paper reported the degumming conditions of A. yamamai using sodium oleate, high pressure and temperature, and sodium carbonate. Degumming ratio of A. yamamai cocoon using sodium oleate was less than 10%. High pressure and temperature treatment induced 30% weight loss of A. yamamai cocoon. The concentration, treatment temperature and time using sodium carbonate was examined and revealed the following conditions for degumming; 5% owf, 60 min at 100℃. The degummed solution was confirmed using UV and FT-IR spectrometer. Our results can be used to handle A. yamamai silkworm cocoon for further application including material processing.","PeriodicalId":14140,"journal":{"name":"International journal of industrial entomology","volume":"45 1","pages":"127-131"},"PeriodicalIF":0.0,"publicationDate":"2015-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80257319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Jang, Byung‐Dae Park, H. Kweon, You-Young Jo, I. Um
{"title":"Preparation, structure, and properties of cellulose nanofibril/silk sericin composite film","authors":"M. Jang, Byung‐Dae Park, H. Kweon, You-Young Jo, I. Um","doi":"10.7852/IJIE.2015.31.1.1","DOIUrl":"https://doi.org/10.7852/IJIE.2015.31.1.1","url":null,"abstract":"Recently, sericin has attracted increasing attention in biomedical and cosmetic research because of its useful properties including acceleration of wound healing, improvement of cell attachment, and inhibition of ultraviolet-B induced apoptosis. However, sericin films have poor mechanical properties, which restricts the application to those fields. In this study, cellulose nanofibril (CNF)/sericin composite films were fabricated by solvent casting, and the effects of ultrasonication time and CNF content on the solution turbidity, molecular conformation, and film mechanical properties of sericin film were examined. As the ultrasonication time increased, the turbidity of the CNF/sericin suspension decreased. Conversely, as the CNF content increased, the turbidity increased. However, b-sheet crystallization and mechanical properties remained almost unchanged by varying the ultrasonication time and CNF content, indicating that CNF is not effective to improve the mechanical properties of sericin films.","PeriodicalId":14140,"journal":{"name":"International journal of industrial entomology","volume":"13 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2015-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81091655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Joon-Ha Lee, In-woo Kim, Mi-Ae Kim, E. Yun, S. Nam, M. Ahn, Young Bo Lee, Jae‐Sam Hwang
{"title":"Scolopendrasin I: a novel antimicrobial peptide isolated from the centipede Scolopendra subspinipes mutilans","authors":"Joon-Ha Lee, In-woo Kim, Mi-Ae Kim, E. Yun, S. Nam, M. Ahn, Young Bo Lee, Jae‐Sam Hwang","doi":"10.7852/IJIE.2015.31.1.14","DOIUrl":"https://doi.org/10.7852/IJIE.2015.31.1.14","url":null,"abstract":"In a previous report, we identified several candidate antimicrobial peptides through de novo RNA sequencing of the centipede Scolopendra subspinipes mutilans . Here, we identify and characterize one of these peptides, Scolopendrasin I. We identified the centipede antimicrobial peptide Cecropin from the centipede transcriptome using an SVM algorithm, and subsequently analyzed the amino acid sequence for predicted secondary structure using a GOR algorithm. We identified an alpha helical region of Cecropin and named it Scolopendrasin I. We then assessed antimicrobial and hemolytic activity of Scolopendrasin I. Scolopendrasin I showed antimicrobial activity against various microbes, including antibiotic-resistant Gram-negative bacteria, in a radial diffusion assay. Scolopendrasin I had potent antibacterial activity against acne-associated microbes in a colony count assay and showed no hemolytic activity in a hemolysis assay. In addition, we confirmed that Scolopendrasin I bound to the surface of bacteria via a specific interaction with lipoteichoic acid and lipopolysaccharide, two components of bacterial cell membranes. In conclusion, the results presented here provide evidence that this is an efficient strategy for antimicrobial peptide candidate identification and that Scolopendrasin I has potential for successful antibiotic development.","PeriodicalId":14140,"journal":{"name":"International journal of industrial entomology","volume":"14 1","pages":"14-19"},"PeriodicalIF":0.0,"publicationDate":"2015-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76089647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sung-kuk Kim, You-Young Jo, Kwang-gill Lee, Hyun-bok Kim, Yong Soon Kim, Wan-Taek Ju, Da-eun Jung, H. Kweon
{"title":"Modification of the characteristics of silkworm powder by treatment with alkaline protease","authors":"Sung-kuk Kim, You-Young Jo, Kwang-gill Lee, Hyun-bok Kim, Yong Soon Kim, Wan-Taek Ju, Da-eun Jung, H. Kweon","doi":"10.7852/IJIE.2015.31.1.30","DOIUrl":"https://doi.org/10.7852/IJIE.2015.31.1.30","url":null,"abstract":"Enzymatic modification of proteins is often used to increase the biological activity of materials. Silkworm powder has been investigated as a functional food resource, but no study has been performed on its modification by commercial food enzyme. Therefore, this study aimed to determine the feasibility of such modification of silkworm powder by alkaline protease. The activity of the enzyme was confirmed using an azocasein assay. Subsequently the silkworm powder was hydrolyzed by enzymatic treatment. UV visible spectrometry showed that the supernatant of silkworm powder subjected to enzymatic treatment had a stronger absorption band than the untreated powder. SDS-PAGE electrophoresis showed that the molecular weight of silkworm powder decreased on enzymatic treatment. Thus the results indicate that commercial enzymes might be used to modify the characteristics of silkworm powder.","PeriodicalId":14140,"journal":{"name":"International journal of industrial entomology","volume":"16 1","pages":"30-33"},"PeriodicalIF":0.0,"publicationDate":"2015-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76195770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kyu‐Won Kwak, S. Nam, Hyo-Won Choi, Ji-Young Choi, Seokhyung Lee, H. Kim, Myung‐Sae Han, K. Park
{"title":"Entomopathogenic fungal infection of Allomyrina dichotoma in mass breeding conditions","authors":"Kyu‐Won Kwak, S. Nam, Hyo-Won Choi, Ji-Young Choi, Seokhyung Lee, H. Kim, Myung‐Sae Han, K. Park","doi":"10.7852/IJIE.2015.31.1.20","DOIUrl":"https://doi.org/10.7852/IJIE.2015.31.1.20","url":null,"abstract":"Increased use of rhinoceros beetles (Allomyrina dichotoma) for food and medicine, and their elevated value as pets has led to an increase in the number of their breeding farms. Mass breeding of the insects in these farms leads to entomological diseases. In this study, we investigate cannibalism resulting from overcrowded breeding of A. dichotoma larvae, as well as secondary fungal infections in epidermal wounds in the surviving larvae. Some of the fungi detected in the present study showed entomopathogenicity, and the larvae showed different times of death. In particular, larvae infected with Clonostachys sp., an endophytic plant fungus, grew for a long time after infection, but died without pupating.","PeriodicalId":14140,"journal":{"name":"International journal of industrial entomology","volume":"13 1","pages":"20-24"},"PeriodicalIF":0.0,"publicationDate":"2015-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77088570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Kim, K. Park, Seokhyung Lee, Kyu‐Won Kwak, Munyoung Choi, Ji-Young Choi
{"title":"Comparison of clay and charcoal as feed additives for Protaetia brevitarsis (Coleoptera: Scarabaeidae)","authors":"H. Kim, K. Park, Seokhyung Lee, Kyu‐Won Kwak, Munyoung Choi, Ji-Young Choi","doi":"10.7852/IJIE.2015.31.1.25","DOIUrl":"https://doi.org/10.7852/IJIE.2015.31.1.25","url":null,"abstract":"The white-spotted chafer, Protaetia brevitarsis (Coleoptera: Scarabaeidae), has been traditionally used in Korea as a medicine for preventing liver-related diseases and suppressing liver cancer. Therefore, this insect is economically important and is commercially reared and sold in Korea. Recently, P. brevitarsis was listed as a temporal food ingredient by the Korean Ministry of Food and Drug Safety. Given the increasing economic importance of this beetle, we have sought to improve rearing conditions for its commercial production. In this study, we compared the effects of two food supplements, clay and charcoal, on the growth of second instar larvae of P. brevitarsis . Clay and charcoal are generally known as good adsorbent for removal of contaminating substances in insect feed. We fed second instar P. brevitarsis larvae a commercial diet consisting of fermented sawdust with seven different combinations of clay and/or activated charcoal, and measured their effects on weight gain for approximately 17 wk until larvae pupated. We found that addition of clay at 2.5% w/w of the fermented sawdust diet had no negative effect on weight gain of second instar P. brevitarsis larvae and thus may improve the quality of P. brevitarsis as a commercial food.","PeriodicalId":14140,"journal":{"name":"International journal of industrial entomology","volume":"2800 1","pages":"25-29"},"PeriodicalIF":0.0,"publicationDate":"2015-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86496933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
So Yun Kim, Haechul Park, I. Park, K. Park, Namjung Kim, Seonghyun Kim
{"title":"Analysis of psychological effects expected from educational pet insect rearing","authors":"So Yun Kim, Haechul Park, I. Park, K. Park, Namjung Kim, Seonghyun Kim","doi":"10.7852/IJIE.2015.31.1.7","DOIUrl":"https://doi.org/10.7852/IJIE.2015.31.1.7","url":null,"abstract":"In an effort to obtain foundational data required to evaluate the therapeutic effect of educational insect rearing, this study surveyed current educational pet insect rearing practices in Korea and examined the expected psychological effects from participating in the activity. Data were collected from those who visited the special insect exhibition in May 2013 using a self-reporting survey. The results indicated that 48.3% of the responders had experienced insect rearing, and that rhino and stag beetles were the most commonly reared insects. Most of the respondents (83.1%) reported they were not currently rearing any insects, which suggested that insect rearing tends to be a one-time experience. Expected psychological effects could be divided into emotional (56.3%) and cognitive (41.8%) effects, and these varied by age and gender. The differences in expected psychological effects were particularly clear between adults and adolescents, the latter of whom were the main participants in the activity. It is therefore necessary to develop standardized manuals and care programs for more diverse insect species with which to help keep up consumer interest in educational insect rearing. These efforts will help champion the psychotherapeutic effects of educational pet insect rearing and enhance its role as a learning aid.","PeriodicalId":14140,"journal":{"name":"International journal of industrial entomology","volume":"416 1","pages":"7-13"},"PeriodicalIF":0.0,"publicationDate":"2015-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75254006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kyu‐Won Kwak, Myung‐Sae Han, S. Nam, Ji-Young Choi, Seokhyung Lee, H. Kim, K. Park
{"title":"Comparing the mortality of Protaetia brevitarsis seulensis (Coleoptera: Cetoniidae) caused by entomopathogenic bacteria and Serratia marcescens (Enterobacteriales: Enterobacteriaceae)","authors":"Kyu‐Won Kwak, Myung‐Sae Han, S. Nam, Ji-Young Choi, Seokhyung Lee, H. Kim, K. Park","doi":"10.7852/IJIE.2015.30.2.40","DOIUrl":"https://doi.org/10.7852/IJIE.2015.30.2.40","url":null,"abstract":"To investigate whether Serratia marcescens (Enterobacteriales: Enterobacteriaceae) isolated from Protaetia brevitarsis seulensis(Coleoptera: Cetoniidae) acts as an opportunistic bacterium in peroral infection, the primary entomopathogenic bacteria Bacillus thuringiensis (Bacillales: Bacillaceae) and Paenibacillus popilliae (Eubacteriales: Bacillaceae) were added to sawdust to perform a bioassay experiment. We found that peroral infection caused by S. marcescens could be fatal beyond a concentration of 4 × 10 8 pfu/mL in 2 nd stage P. b. seulensis larvae","PeriodicalId":14140,"journal":{"name":"International journal of industrial entomology","volume":"27 1","pages":"40-44"},"PeriodicalIF":0.0,"publicationDate":"2015-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79480606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of forestry host plants, rearing seasons and their interaction on cocoon productivity of tropical tasar silkworm, Antheraea mylitta in uttarakhand","authors":"N. K. Bhatia, M. Yousuf","doi":"10.7852/IJIE.2015.30.2.31","DOIUrl":"https://doi.org/10.7852/IJIE.2015.30.2.31","url":null,"abstract":"","PeriodicalId":14140,"journal":{"name":"International journal of industrial entomology","volume":"10 1","pages":"31-39"},"PeriodicalIF":0.0,"publicationDate":"2015-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78574308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}