International journal of food microbiology最新文献

筛选
英文 中文
The putative Rho guanine nucleotide exchange factor GerA in Aspergillus flavus regulates growth, development, and aflatoxin synthesis 推测的Rho鸟嘌呤核苷酸交换因子GerA在黄曲霉调节生长,发育和黄曲霉毒素合成
IF 5 1区 农林科学
International journal of food microbiology Pub Date : 2025-06-05 DOI: 10.1016/j.ijfoodmicro.2025.111302
Jia Xu , Junhe Ren , Yanyan Zhang, Zhuoyu Han, Qing Kong
{"title":"The putative Rho guanine nucleotide exchange factor GerA in Aspergillus flavus regulates growth, development, and aflatoxin synthesis","authors":"Jia Xu ,&nbsp;Junhe Ren ,&nbsp;Yanyan Zhang,&nbsp;Zhuoyu Han,&nbsp;Qing Kong","doi":"10.1016/j.ijfoodmicro.2025.111302","DOIUrl":"10.1016/j.ijfoodmicro.2025.111302","url":null,"abstract":"<div><div><em>Aspergillus flavus</em> is a saprophytic fungus that infects seed crops before and after harvest, producing potent carcinogens known as aflatoxins. Consequently, the urgent need arises to develop control strategies to mitigate the adverse effects associated with <em>A. flavus</em>. The Ras homologue family member GTPases (Rho GTPases) play a crucial role in regulating the developmental and pathogenic processes of filamentous fungi by controlling various signal transduction pathways. Rho guanine nucleotide exchange factors (GEFs) activate Rho GTPases and regulate their conformational conversion from GDP to GTP. In a previous study, a Rho GEF GerA that is closely regulated by the global regulator VeA under different culture conditions was identified by chromatin immunoprecipitation and sequencing (ChIP-seq), which contains a characteristic Dbl homology (DH) domain. GerA exhibits a high degree of conservation within <em>Aspergillus</em> species but lacks homologues in <em>Saccharomyces cerevisiae</em>. The generation of the Δ<em>gerA</em> mutant using CRISPR/Cas9 gene editing tools significantly impacted the synthesis of AFs, reproductive processes, and pathogenicity in <em>A. flavus</em>. Notably, the Δ<em>gerA</em> mutant exhibited polarity loss and impaired septation. Combined with yeast two-hybrid analysis, there is speculation that GerA may function as a Rho4 GEF. The significant involvement of <em>gerA</em> in the morphological development and secondary metabolism of <em>A. flavus</em> highlights its potential as a target for control measures. Moreover, this study establishes a basis for further investigating the roles of other Rho GEFs in filamentous fungi.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"441 ","pages":"Article 111302"},"PeriodicalIF":5.0,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144239850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Two novel Vibrio parahaemolyticus phages (vB_VpaS_1601 and vB_VpaP_1701) can serve as potential biocontrol agents against Vibrio parahaemolyticus infection in seafood 两种新型副溶血性弧菌噬菌体vB_VpaS_1601和vB_VpaP_1701可作为潜在的海产品副溶血性弧菌生物防治剂
IF 5 1区 农林科学
International journal of food microbiology Pub Date : 2025-06-04 DOI: 10.1016/j.ijfoodmicro.2025.111303
Zhenxiang Tao , Yue Sun , Mengxiao Zhou , Tengteng Li , Weiqing Lan , Yong Zhao , Xiaohong Sun
{"title":"Two novel Vibrio parahaemolyticus phages (vB_VpaS_1601 and vB_VpaP_1701) can serve as potential biocontrol agents against Vibrio parahaemolyticus infection in seafood","authors":"Zhenxiang Tao ,&nbsp;Yue Sun ,&nbsp;Mengxiao Zhou ,&nbsp;Tengteng Li ,&nbsp;Weiqing Lan ,&nbsp;Yong Zhao ,&nbsp;Xiaohong Sun","doi":"10.1016/j.ijfoodmicro.2025.111303","DOIUrl":"10.1016/j.ijfoodmicro.2025.111303","url":null,"abstract":"<div><div><em>Vibrio parahaemolyticus</em> poses a serious threat to the aquaculture industry and human health. Two novel phages (vB_VpaS_1601 and vB_VpaP_1701) were isolated from oysters. Phage vB_VpaS_1601 and phage vB_VpaP_1701 exhibited short latent periods and high burst sizes. In addition, both phages showed stability across a broad range of temperature and pH conditions. Genomic analyses revealed that phage vB_VpaS_1601 is a novel genus of the <em>Caudoviricetes</em> class, and vB_VpaP_1701 is a new species of the <em>Maculvirus</em> genus, <em>Autographiviridae</em> family. Both single phage and phage cocktail can inhibit <em>V. parahaemolyticus</em> proliferation, with phage cocktail (MOI = 1) can completely inhibit the growth of <em>V. parahaemolyticus</em> within 12 h. The phages reduced biofilm formation by 53.57 % (vB_VpaS_1601) and 64.88 % (vB_VpaP_1701), while the cocktail achieved 46 %–78 % inhibition. Phage cocktail can reduce <em>V. parahaemolyticus</em> by 1.53–2.74 log CFU/cm<sup>3</sup> in salmon and 1.56–2.91 log CFU/cm<sup>3</sup> in oysters. These results indicated that vB_VpaS_1601 and vB_VpaP_1701 have potential as antibacterial agents for controlling <em>V. parahaemolyticus</em> in seafood products.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"441 ","pages":"Article 111303"},"PeriodicalIF":5.0,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144223400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection of Fusarium spp. and T-2 and HT-2 toxins contamination in oats using visible and near-infrared spectroscopy 可见光和近红外光谱法检测燕麦中镰刀菌及T-2和HT-2毒素污染
IF 5 1区 农林科学
International journal of food microbiology Pub Date : 2025-06-04 DOI: 10.1016/j.ijfoodmicro.2025.111301
Irene Teixido-Orries , Lexuan Yang , Francisco Molino , Angel Medina , Sonia Marín , Carol Verheecke-Vaessen
{"title":"Detection of Fusarium spp. and T-2 and HT-2 toxins contamination in oats using visible and near-infrared spectroscopy","authors":"Irene Teixido-Orries ,&nbsp;Lexuan Yang ,&nbsp;Francisco Molino ,&nbsp;Angel Medina ,&nbsp;Sonia Marín ,&nbsp;Carol Verheecke-Vaessen","doi":"10.1016/j.ijfoodmicro.2025.111301","DOIUrl":"10.1016/j.ijfoodmicro.2025.111301","url":null,"abstract":"<div><div><em>Fusarium langsethiae</em> (FL) is one of the major contaminants in oats in the United Kingdom (UK) and is a significant producer of T-2 and HT-2 toxins, among the most prevalent mycotoxins in oats. Visible and near-infrared (Vis-NIR) (350–2500 nm) spectroscopy was explored as a non-invasive, rapid method for detecting FL, <em>Fusarium</em> species that produce T-2 and HT-2 toxins, and T-2 and HT-2 toxins content. Oat grains were artificially inoculated with FL and other <em>Fusarium</em> species under controlled water activity (a<sub>w</sub>) conditions (0.98, 0.90, and 0.80). FL was found to be particularly responsible for producing T-2 and HT-2 toxins. Classification models were developed to distinguish oat grains based on the presence of FL. The best performance was achieved with all the Vis-NIR spectra, with a classification accuracy of 76.2 %. The Vis region (350–995 nm) emerged as the most important range for classification. Additionally, oat grains were classified by T-2 and HT-2 toxin content, distinguishing oats above and below the European Union (EU) threshold with 93.3 % accuracy. For mycotoxin quantification, the best performance was obtained using the Vis region with a coefficient of determination (R<sup>2</sup>) of 0.875. Key wavelengths such as 464, 568, 575 and 636 nm were relevant for toxin detection. The NIR region (1005–1795 nm) also played a significant role in the models. This study shows that Vis-NIR spectroscopy is a promising, non-destructive tool for detecting <em>Fusarium</em> and type A trichothecenes in oats, though further research is needed to improve model robustness and support food safety monitoring.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"441 ","pages":"Article 111301"},"PeriodicalIF":5.0,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144223399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dynamic changes in fungal composition during the processing of Hainan areca nut and effects of water activity on dominant mold species 海南槟榔加工过程中真菌组成的动态变化及水分活性对优势霉菌种类的影响
IF 5 1区 农林科学
International journal of food microbiology Pub Date : 2025-06-03 DOI: 10.1016/j.ijfoodmicro.2025.111300
Menjun Zhu , Juanjuan Li , Xinjing Li , Yishun Yao , Xing Xu , Khizar Hayat , Fengwei Tian , Xiaoming Zhang , Chi-Tang Ho
{"title":"Dynamic changes in fungal composition during the processing of Hainan areca nut and effects of water activity on dominant mold species","authors":"Menjun Zhu ,&nbsp;Juanjuan Li ,&nbsp;Xinjing Li ,&nbsp;Yishun Yao ,&nbsp;Xing Xu ,&nbsp;Khizar Hayat ,&nbsp;Fengwei Tian ,&nbsp;Xiaoming Zhang ,&nbsp;Chi-Tang Ho","doi":"10.1016/j.ijfoodmicro.2025.111300","DOIUrl":"10.1016/j.ijfoodmicro.2025.111300","url":null,"abstract":"<div><div>Areca nut, one of the most historically representative chewing products in China, faces challenges from harmful microbial growth that causes both product safety and quality problems. To elucidate the mechanism underlying these microbial threats and develop targeted control strategies, this study analyzed dynamic microbial profiles of Hainan areca nuts during the processing stages. Alpha diversity analysis and total fungal counts (TFC) of Hainan areca nuts were monitored during different processing stages. A significant increase in TFC (1.725 ± 0.046 Lg CFU/g) and diversity of fungal species was observed in the finished product (FP), which might be attributed to manual operation and environmental exposure during the final processing stages. Ten strains of mold with the highest TFC were isolated and further screened on the areca nut powder culture medium. Two dominant mold species were isolated and identified by phylogenetic analysis, and were demonstrated to be <em>Aspergillus ruber</em> and <em>Aspergillus ochraceus</em>. Growth curves under varying water activities (A<sub>w</sub>) indicated that the grows of two dominant mold species were inhibited at A<sub>w</sub> of 0.798 and 0.815, respectively. Reinoculation of these species in areca nut products (A<sub>w</sub> 0.80) further demonstrated that reduced A<sub>w</sub> significantly suppressed their growth rates. These results may critically contribute to the prediction and extension of the shelf-life of areca nut products.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"440 ","pages":"Article 111300"},"PeriodicalIF":5.0,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144195080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fluoroquinolone susceptibility of wild-type Listeria monocytogenes isolates and the role of FepR and ParC mutations in conferring fluoroquinolone tolerance 野生型单核增生李斯特菌对氟喹诺酮的敏感性及FepR和ParC突变在氟喹诺酮耐受性中的作用
IF 5 1区 农林科学
International journal of food microbiology Pub Date : 2025-06-03 DOI: 10.1016/j.ijfoodmicro.2025.111290
Andrea Domen , Jenna Porter , Lorraine McIntyre , Joy Waite-Cusic , Jovana Kovacevic
{"title":"Fluoroquinolone susceptibility of wild-type Listeria monocytogenes isolates and the role of FepR and ParC mutations in conferring fluoroquinolone tolerance","authors":"Andrea Domen ,&nbsp;Jenna Porter ,&nbsp;Lorraine McIntyre ,&nbsp;Joy Waite-Cusic ,&nbsp;Jovana Kovacevic","doi":"10.1016/j.ijfoodmicro.2025.111290","DOIUrl":"10.1016/j.ijfoodmicro.2025.111290","url":null,"abstract":"<div><div>Fluoroquinolone response and associated genotypic variants were characterized in a set of 88 <em>Listeria monocytogenes</em> strains isolated from Canadian dairy facilities over a span of 10 years. All strains were susceptible to ciprofloxacin (CIP), moxifloxacin (MOX), and levofloxacin (LEV) using the VITEK GP-AST75 assay. However, CIP minimum inhibitory concentrations (MICs) varied in the broth dilution assay, with 47 isolates exhibiting MICs of 1 ppm and 41 isolates at ≤0.5 ppm (detection limit). Isolates with elevated MICs to one fluoroquinolone showed correlated increases in MICs to other fluoroquinolones (Spearman's correlation; <em>p</em> &lt; 0.0001). MICs were confirmed using a broth dilution assay (0.125–8 ppm; two-fold dilution in MHB), with variable growth observed among replicates at sublethal CIP concentrations. After two passages in 0.5 ppm CIP, WRLP81 exhibited increased fitness (reduced lag phase duration) and MIC increases in CIP (4 ppm), LEV (2 ppm), and MOX (1 ppm). Whole genome sequencing of descendants (81.2B; 81.2C) revealed two unique frameshift mutations in <em>fepR</em>: V114fs and Glu139fs. Molecular modeling predicted conformational defects likely impairing FepA dimerization and efflux pump repression. Similarly, after two passages in 0.5 ppm CIP, a WRLP86 descendant (86.2C_2) exhibited increased MICs for CIP (2 ppm), LEV (4 ppm), and MOX (1 ppm), linked to a <em>parC</em> missense mutation that altered the winged helix domain. This amino acid change likely creates steric interference near the fluoroquinolone binding pocket and represents the first report of a <em>parC</em> mutation identified in <em>L. monocytogenes</em> in response to sublethal CIP exposure. This research highlights the rapid and varied adaptation of reduced fluoroquinolone susceptibility in <em>L. monocytogenes</em>.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"441 ","pages":"Article 111290"},"PeriodicalIF":5.0,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144223401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Changes in microbial assembly and ecological processes following application of Aureobasidium pullulans on apple fruit surface 施用普鲁兰小孢子菌对苹果果实表面微生物聚集和生态过程的影响
IF 5 1区 农林科学
International journal of food microbiology Pub Date : 2025-06-03 DOI: 10.1016/j.ijfoodmicro.2025.111299
V. Yeka Zhimo , Vijay Kumar Sharma , Oleg Feygenberg , Shoshana Salim , Susan R. Whitehead , Davide Spadaro , Shiri Freilich , Michael Wisniewski , Samir Droby
{"title":"Changes in microbial assembly and ecological processes following application of Aureobasidium pullulans on apple fruit surface","authors":"V. Yeka Zhimo ,&nbsp;Vijay Kumar Sharma ,&nbsp;Oleg Feygenberg ,&nbsp;Shoshana Salim ,&nbsp;Susan R. Whitehead ,&nbsp;Davide Spadaro ,&nbsp;Shiri Freilich ,&nbsp;Michael Wisniewski ,&nbsp;Samir Droby","doi":"10.1016/j.ijfoodmicro.2025.111299","DOIUrl":"10.1016/j.ijfoodmicro.2025.111299","url":null,"abstract":"<div><div>The apple fruit surface hosts a diverse microbiome that plays a vital role in fruit health and resistance to postharvest decay. <em>Aureobasidium pullulans</em>, a core member of this microbiome, has been extensively studied for its biocontrol properties, yet its ecological role in shaping microbial community dynamics remains poorly understood. This study aimed to evaluate the effect of manipulating a native <em>Aureobasidium</em>, to act both as a biocontrol agent and as a modulator of the epiphytic microbiome when applied at different stages of fruit development and storage. Among 28 <em>Aureobasidium</em> isolates recovered from ‘Golden Delicious’ apple surfaces, A69 demonstrated the most potent antagonistic activity against <em>Penicillium expansum</em> through volatile and diffusible metabolite production and effective suppression of lesion development in fruit. While preharvest field applications had limited effects on microbial diversity, postharvest and combined treatments significantly reshaped both fungal and bacterial communities on the apple fruit surface. Notably, A69 treatments inhibited decay incidence in stored fruit, reduced fungal diversity and enriched beneficial bacterial and yeast genera including <em>Vishniacozyma</em>, <em>Cystobasidium</em>, <em>Buckleyzyma</em>, <em>Bacillus</em>, and <em>Frigoribacterium</em>, many of which exhibited antagonistic activity against key postharvest pathogens. Cross-kingdom network and ecological assembly analyses revealed that A69 promoted deterministic selection within fungal communities and fostered competitive microbial interactions, resulting in a more stable and resilient microbiome. In contrast, bacterial communities remained largely governed by stochastic processes. These findings highlight the role of A69 as an effective biocontrol agent reducing decay while fostering a disease-suppressive microbiome, providing an eco-friendly microbiome-based strategy for sustainable postharvest disease control.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"441 ","pages":"Article 111299"},"PeriodicalIF":5.0,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144223398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metatranscriptomics and HS-GC-IMS unveils the disparities in gene functions, metabolic traits, and volatile metabolites between highly-preserved and easily-spoiled soybean pastes met转录组学和HS-GC-IMS揭示了高度保存和容易变质的大豆酱在基因功能、代谢性状和挥发性代谢物方面的差异
IF 5 1区 农林科学
International journal of food microbiology Pub Date : 2025-06-02 DOI: 10.1016/j.ijfoodmicro.2025.111291
Fei Ren, Yanxiang Liu, Bin Tan
{"title":"Metatranscriptomics and HS-GC-IMS unveils the disparities in gene functions, metabolic traits, and volatile metabolites between highly-preserved and easily-spoiled soybean pastes","authors":"Fei Ren,&nbsp;Yanxiang Liu,&nbsp;Bin Tan","doi":"10.1016/j.ijfoodmicro.2025.111291","DOIUrl":"10.1016/j.ijfoodmicro.2025.111291","url":null,"abstract":"<div><div>Traditional fermented soybean paste samples display distinct highly-preserved (HP) and easily-spoiled (ES) characteristics. This study delves into the metatranscriptomic and volatile metabolites investigations of HP and ES samples to explore their influencing on the quality of soybean pastes. The results showed that Firmicutes were predominant in HP soybean pastes, in which genus <em>Bacillus</em> having highest abundance, while Proteobacteria was most abundant in ES ones, with <em>Citrobacter</em>, <em>Salmonella</em>, and <em>Escherichia</em> top abundant. Amino acid transport and metabolism, carbohydrate transport and metabolism, energy production and conversion held most abundant gene functions. HP and ES samples had significant differences in biological processes, cellular components, and molecular functions revealed with GO functional enrichment analysis. Furthermore, ABC transporters and aminoacyl-tRNA biosynthesis were among significantly enriched pathways in HP samples, and biosynthesis of secondary metabolites and chlorocyclohexane and chlorobenzene degradation in ES samples disclosed with pathway enrichment analysis with KEGG. Among ES downregulated genes, the most function-related genes belonged to <em>Bacillus.</em> The selected genes data with qRT-PCR effectively validated the metatranscriptomic results. For volatile compounds, HP had much more 1-octanal, 1-octen-3-ol, ethanol, limonene, ethyl lactate and ethyl acetate, ES soybean pastes had higher levels of propanal, hexanal, 3-methyl butanoic acid, methanol. The research furnishes a comprehension of the microbes, functional genes and volatile metabolites involved in soybean paste preservative properties, which may help improve the quality of soybean paste products.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"441 ","pages":"Article 111291"},"PeriodicalIF":5.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144203888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rapid and quantitative detection of Botryosphaeria dothidea by surface-enhanced Raman spectroscopy with size-controlled spherical metal nanoparticles combined with machine learning 尺寸控制球形金属纳米颗粒结合机器学习的表面增强拉曼光谱快速定量检测斑点球孢菌
IF 5 1区 农林科学
International journal of food microbiology Pub Date : 2025-05-30 DOI: 10.1016/j.ijfoodmicro.2025.111288
Longhui Luo , Wei Tian , Qian Liu , Xiaoying Yang , Tingting Chen , Zhibo Zhao , Xiufang Yan , Chao Kang , Dongmei Chen , Youhua Long
{"title":"Rapid and quantitative detection of Botryosphaeria dothidea by surface-enhanced Raman spectroscopy with size-controlled spherical metal nanoparticles combined with machine learning","authors":"Longhui Luo ,&nbsp;Wei Tian ,&nbsp;Qian Liu ,&nbsp;Xiaoying Yang ,&nbsp;Tingting Chen ,&nbsp;Zhibo Zhao ,&nbsp;Xiufang Yan ,&nbsp;Chao Kang ,&nbsp;Dongmei Chen ,&nbsp;Youhua Long","doi":"10.1016/j.ijfoodmicro.2025.111288","DOIUrl":"10.1016/j.ijfoodmicro.2025.111288","url":null,"abstract":"<div><div><em>Botryosphaeria dothidea</em> infection has become a major factor affecting the quality of postharvest fruits, so detection of <em>B. dothidea</em> infection is very important to control the spread of infection and ensure food safety. In this study, we built a monitoring platform for rapid and quantitative detection of <em>B. dothidea</em> by Surface-enhanced Raman spectroscopy (SERS) with size-controlled spherical metal nanoparticles combined with machine learning, and the platform could also detect a variety of pathogens. With spherical metal nanoparticles as the active substrate, the SERS enhanced effect was significantly size-dependent. 45–60 nm Ag@ICNPs was determined to achieve the maximum SERS signal detection of <em>B. dothidea</em>, and used as the active substrate to construct a SERS platform for rapid and quantitative detection of <em>B. dothidea</em>. This platform had potential practicability in actual sample detection. The platform was used for the detection of <em>Pseudomonas syringae pv. Actinidiae</em>, <em>Pseudomonas aeruginosa</em>, <em>Ralstonia solanacearum</em> and <em>Pseudomonas extremorientalis</em>, and the SERS fingerprint information of these pathogens was successfully captured, and the quantitative analysis ability of these pathogens was also strong. Machine learning analysis was performed on the SERS spectra of pathogens obtained. Based on the differences between the spectral data sets of different pathogens, PCA could effectively distinguish these five pathogens into different groups. The accuracy rates of SVM, Tree, Linear discrimination analysis, Efficient logistic regression, Naive Bayes, K-Nearest Neighbors, Ensemble and Neural network test were 100 %, 96 %, 100 %, 100 %, 100 %, 100 %, 98 % and 100 % respectively, all of which had relatively high accuracy rates. Overall, this study provides a simple, efficient and accurate method for rapid quantitative detection and identification of multiple pathogens, and can be extended to practical agricultural products.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"440 ","pages":"Article 111288"},"PeriodicalIF":5.0,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144185773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Endophytic Diaporthe citrichinensis as a new source to alleviate post-harvest fungal infections in apple (Malus x domestica Borkh.) 缓解苹果采后真菌感染的内生植物柠檬酸双胞菌(Diaporthe citrichinensis)
IF 5 1区 农林科学
International journal of food microbiology Pub Date : 2025-05-30 DOI: 10.1016/j.ijfoodmicro.2025.111289
Shreya Gupta, Sanjai Saxena
{"title":"Endophytic Diaporthe citrichinensis as a new source to alleviate post-harvest fungal infections in apple (Malus x domestica Borkh.)","authors":"Shreya Gupta,&nbsp;Sanjai Saxena","doi":"10.1016/j.ijfoodmicro.2025.111289","DOIUrl":"10.1016/j.ijfoodmicro.2025.111289","url":null,"abstract":"<div><div>After harvesting, Apple is susceptible to being attacked by various fungal pathogens, rendering it unconsumable and further reducing its commercial value. To mitigate these losses, biocontrol strategies utilizing endophytic fungi are gaining momentum as effective alternatives to synthetic fungicides. In the present study, 155 endophytic fungi were isolated from various parts of <em>Malus</em> x <em>domestica</em> Borkh., sourced from Mandi (Himachal Pradesh) and Pulwama (Jammu &amp; Kashmir). The isolates were subjected to screening for antifungal activity against the primary post-harvest pathogens of apple including, <em>Alternaria alternata</em>, <em>Aspergillus niger</em>, <em>Botrytis cinerea</em>, <em>Penicillium expansum</em>, <em>Rhizopu</em>s sp., and <em>Venturia inaequalis</em>. In the dual-confrontation test, the isolates 03MDLP P3–3 and 01MDLM P1–6 exhibited the highest percentage inhibition against all target phytopathogens. The potent cultures were further scrutinized using sandwich assay, wherein 03MDLP P3–3 exhibited complete growth inhibition against all phytopathogenic fungi, indicating its antifungal efficacy, likely attributed to its capacity to release volatile organic compounds. Using morphological and molecular techniques, 03MDLP P3–3 was identified as <em>Diaporthe citrichinensis</em>. This study marks the first report of isolating <em>D. citrichinensis</em> as an endophyte from apples. Furthermore, a pathogenicity test was conducted on the potent isolate, revealing no harmful effects when introduced to apple fruits according to the Koch's postulate. The effect of <em>D. citrichinensis</em> was also examined under in vivo conditions against the major post-harvest fungal pathogens of apple fruits, revealing that no lesion development was noted on the test fruits inoculated with spore suspension of pathogens in the presence of <em>D. citrichinensis</em>. The present study offers a promising avenue for utilizing <em>D. citrichinensis</em> as a biocontrol agent to combat post-harvest fungal diseases in apple production.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"440 ","pages":"Article 111289"},"PeriodicalIF":5.0,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144195079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Unexpected high prevalence of carbapenemase-producing bacteria and widely spread of blaNDM-1-positive Shewanella spp. in retail shrimp 产碳青霉烯酶细菌在零售虾中意外的高流行率和blandm -1阳性希瓦氏菌的广泛传播
IF 5 1区 农林科学
International journal of food microbiology Pub Date : 2025-05-29 DOI: 10.1016/j.ijfoodmicro.2025.111284
Chao Yue , Xun Gao , Ludan Xiao, Litao Lu, Jiakuo Chen, Caiying Lin, Zhongpeng Cai, Guolong Gao, Luchao Lv, Jian-Hua Liu, Yi-Yun Liu
{"title":"Unexpected high prevalence of carbapenemase-producing bacteria and widely spread of blaNDM-1-positive Shewanella spp. in retail shrimp","authors":"Chao Yue ,&nbsp;Xun Gao ,&nbsp;Ludan Xiao,&nbsp;Litao Lu,&nbsp;Jiakuo Chen,&nbsp;Caiying Lin,&nbsp;Zhongpeng Cai,&nbsp;Guolong Gao,&nbsp;Luchao Lv,&nbsp;Jian-Hua Liu,&nbsp;Yi-Yun Liu","doi":"10.1016/j.ijfoodmicro.2025.111284","DOIUrl":"10.1016/j.ijfoodmicro.2025.111284","url":null,"abstract":"<div><div>Carbapenems, critical last-resort antibiotics, are increasingly ineffective due to the spread of carbapenemase-producing pathogens, particularly New Delhi metallo-β-lactamase (NDM)-producing bacteria. Although NDM has been detected in a variety of environmental and clinical sources, its prevalence in seafood, especially shrimp, remains underexplored despite shrimp being a widely consumed food. This study aimed to investigate the prevalence and molecular characteristics of NDM-producing bacteria in shrimp. A total of 77 non-repetitive shrimp samples were collected from 8 farmers' markets to detect NDM-producing bacteria. Among these, 38 <em>bla</em><sub>NDM</sub>-positive isolates were recovered from 34 samples (44.2 %, 34/77) and subject to whole genome sequencing (WGS). MALDI-TOF and WGS showed that these isolates were identified as <em>Shewanella</em> spp. (<em>S. indica</em>, <em>n</em> = 9; <em>S. chilikensis</em>, <em>n</em> = 12; <em>S. algae</em>, <em>n</em> = 6), <em>Vibrio fluvialis</em> (n = 6), and <em>Escherichia coli</em> (<em>n</em> = 5), with <em>S.</em> spp. and <em>V. fluvialis</em> exhibiting high genetic homogeneity, suggesting localized clonal spread among shrimps. Further Nanopore sequencing and molecular characterization of 11 randomly selected isolates indicated that ten <em>bla</em><sub>NDM</sub> genes were located on plasmids, including IncC (<em>n</em> = 2, from <em>S. algae</em> and <em>V. furnissii</em>), untypeable plasmids from <em>Shewanella</em> spp. (<em>n</em> = 5), and IncI1, IncHI2, and IncFII-FIA plasmids from <em>E. coli</em>. These plasmids exhibited high homology with those from other sources deposited in GenBank, and the untypeable plasmids were unique to <em>Shewanella</em> spp. This study, to the best of our knowledge, represents the first report of the high prevalence of <em>bla</em><sub>NDM</sub>-positive pathogens in shrimp, highlighting the critical public health threat posed by the widespread propagation of <em>bla</em><sub>NDM</sub> through plasmids and clonal transmission in seafood. This study contributes to the growing body of evidence highlighting the critical need for stringent food safety policies and regulations to combat the health risks posed by emerging food pollutants.</div></div>","PeriodicalId":14095,"journal":{"name":"International journal of food microbiology","volume":"441 ","pages":"Article 111284"},"PeriodicalIF":5.0,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144229483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信