International journal of experimental diabetes research最新文献_第3页

Markers for Risk of Type 1 Diabetes in Relatives of Alsacian Patients With Type 1 Diabetes 阿尔萨斯1型糖尿病患者亲属中1型糖尿病风险的标志物

International journal of experimental diabetes research Pub Date : 2002-01-01 DOI: 10.1080/15604280212527

J. Ongagna, R. Sapin, M. Pinget, A. Belcourt

{"title":"Markers for Risk of Type 1 Diabetes in Relatives of Alsacian Patients With Type 1 Diabetes","authors":"J. Ongagna, R. Sapin, M. Pinget, A. Belcourt","doi":"10.1080/15604280212527","DOIUrl":"https://doi.org/10.1080/15604280212527","url":null,"abstract":"Background: The cytotoxic T lymphocyteassociated antigen 4 gene (CTLA-4) encode the T cell receptor involved in the control of T cell proliferation and mediates T cell apoptosis. The receptor protein is a specific T lymphocyte surface antigen that is detected on cells only after antigen presentation. Thus, CTLA-4 is directly involved in both immune and autoimmune responses and may be involved in the pathogenesis of multiple T cell-mediated autoimmune disorders. There is polymorphism at position 49 in exon 1 of the CTLA-4 gene, providing an A-G exchange. Moreover, we assessed the CTLA-4 49 (Thr/Ala) polymorphism in diabetic patients and first-degree relatives as compared to control subjects. Research design and methods: Three loci (HLA-DQB1, DQA1 and CTLA-4) were analysed in 62 type 1 diabetic patients, 72 firstdegree relatives and 84 nondiabetic control subjects by means of PCR-RFLP. Results: A significant enrichment in DQB1 alleles encoding for an amino acid different from Asp in position 57 (NA) and DQA1 alleles encoding for Arg in position 52 was observed in diabetic subjects and first-degree relatives as compared to controls. The genotype and allele frequencies of these polymorphisms in type 1 diabetic patients and firstdegree relatives differed significantly from those of controls (p< 0.001 and 0.05 respectively). CTLA-49 Ala alleles frequencies were 75.8% in type 1 diabetic patients and 68.1% in first-degree relatives in comparison to 35.7% in control subjects. The Ala/Ala genotype conferred a relative risk of 18.8 (p < 0.001). Conclusion: The CTLA-4 49 Ala allele confers an increased risk of type 1 diabetes, independent of age and HLA-DQ genetic markers.","PeriodicalId":14040,"journal":{"name":"International journal of experimental diabetes research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76019289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

D-Chiro-Inositol – Its Functional Role in Insulin Action and its Deficit in Insulin Resistance d -氨基肌醇在胰岛素作用中的功能作用及其在胰岛素抵抗中的缺陷

International journal of experimental diabetes research Pub Date : 2002-01-01 DOI: 10.1080/15604280212528

J. Larner,

{"title":"D-Chiro-Inositol – Its Functional Role in Insulin Action and its Deficit in Insulin Resistance","authors":"J. Larner,","doi":"10.1080/15604280212528","DOIUrl":"https://doi.org/10.1080/15604280212528","url":null,"abstract":"In this review we discuss the biological significance of D-chiro-inositol, originally discovered as a component of a putative mediator of intracellular insulin action, where as a putative mediator, it accelerates the dephosphorylation of glycogen synthase and pyruvate dehydrogenase, rate limiting enzymes of non-oxidative and oxidative glucose disposal. Early studies demonstrated a linear relationship between its decreased urinary excretion and the degree of insulin resistance present. When tissue contents, including muscle, of type 2 diabetic subjects were assayed, they demonstrated a more general body deficiency. Administration of D-chiro-inositol to diabetic rats, Rhesus monkeys and now to humans accelerated glucose disposal and sensitized insulin action. A defect in vivo in the epimerization of myoinositol to chiro-inositol in insulin sensitive tissues of the GK type 2 diabetic rat has been elucidated. Thus, administered D-chiro-inositol may act to bypass a defective normal epimerization of myo-inositol to D-chiro-inositol associated with insulin resistance and act to at least partially restore insulin sensitivity and glucose disposal.","PeriodicalId":14040,"journal":{"name":"International journal of experimental diabetes research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88919203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 252

Growth Factor-Dependent Proliferation of the Pancreatic β-cell Line βTC-tet: An Assay for β-cell Mitogenic Factors 胰腺β-细胞系βTC-tet生长因子依赖性增殖:β-细胞有丝分裂因子测定

International journal of experimental diabetes research Pub Date : 2002-01-01 DOI: 10.1080/15604280212526

D. Milo-Landesman, S. Efrat

{"title":"Growth Factor-Dependent Proliferation of the Pancreatic β-cell Line βTC-tet: An Assay for β-cell Mitogenic Factors","authors":"D. Milo-Landesman, S. Efrat","doi":"10.1080/15604280212526","DOIUrl":"https://doi.org/10.1080/15604280212526","url":null,"abstract":"The ability to expand normal pancreatic islet β cells in culture would significantly advance the prospects of cell therapy for diabetes. A number of growth factors can stimulate limited islet cell replication, however other factors may exist which are more effective β-cell-specific mitogens. The search for novel β-cell growth factors has been hampered by the lack of a β-cell-specific proliferation assay. We developed a simple and sensitive assay for β-cell growth factors based on a conditionally-transformed mouse β-cell line (βTC-tet). These cells express the SV40 T antigen (Tag) oncoprotein under control of the tetracycline (Tc) operon regulatory system. In the presence of Tc, Tag expression is tightly shut off and the cells undergo complete growth arrest. Here we show that the growth-arrested cells can proliferate in response to growth factors in the absence of Tag. Using this assay, a number of growth factors previously shown to be mitogenic to a mixed islet cell population were found to induce proliferation of pure β cells. We conclude that growth-arrested βTC-tet cells can be employed in a survey of factors from various sources for identifying novel factors with β-cell mitogenic activity.","PeriodicalId":14040,"journal":{"name":"International journal of experimental diabetes research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75384669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Effect of Treating Streptozotocin-Induced Diabetic Rats With Sorbinil, Myo-Inositol or Aminoguanidine on Endoneurial Blood Flow, Motor Nerve Conduction Velocity and Vascular Function of Epineurial Arterioles of the Sciatic Nerve 山梨比尼、肌醇和氨基胍治疗链脲佐菌素诱导的糖尿病大鼠对坐骨神经神经外动脉血流、运动神经传导速度和血管功能的影响

International journal of experimental diabetes research Pub Date : 2002-01-01 DOI: 10.1080/15604280212525

L. Coppey, J. S. Gellett, E. Davidson, J. Dunlap, M. Yorek

{"title":"Effect of Treating Streptozotocin-Induced Diabetic Rats With Sorbinil, Myo-Inositol or Aminoguanidine on Endoneurial Blood Flow, Motor Nerve Conduction Velocity and Vascular Function of Epineurial Arterioles of the Sciatic Nerve","authors":"L. Coppey, J. S. Gellett, E. Davidson, J. Dunlap, M. Yorek","doi":"10.1080/15604280212525","DOIUrl":"https://doi.org/10.1080/15604280212525","url":null,"abstract":"Previously we have demonstrated that diabetes causes impairment in vascular function of epineurial vessels, which precedes the slowing of motor nerve conduction velocity. Treatment of diabetic rats with aldose reductase inhibitors, aminoguanidine or myo-inositol supplementation have been shown to improve motor nerve conduction velocity and/or decreased endoneurial blood flow. However, the effect these treatments have on vascular reactivity of epineurial vessels of the sciatic nerve is unknown. In these studies we examined the effect of treating streptozotocininduced rats with sorbinil, aminoguanidine or myo-inositol on motor nerve conduction velocity, endoneurial blood flow and endothelium dependent vascular relaxation of arterioles that provide circulation to the region of the sciatic nerve. Treating diabetic rats with sorbinil, aminoguanidine or myo-inositol improved the reduction of endoneurial blood flow and motor nerve conduction velocity. However, only sorbinil treatment significantly improved the diabetes-induced impairment of acetylcholinemediated vasodilation of epineurial vessels of the sciatic nerve. All three treatments were efficacious in preventing the appropriate metabolic derangements associated with either activation of the polyol pathway or increased nonenzymatic glycation. In addition, sorbinil was shown to prevent the diabetes-induced decrease in lens glutathione level. However, other markers of oxidative stress were not vividly improved by these treatments. These studies suggest that sorbinil treatment may be more effective in preventing neural dysfunction in diabetes than either aminoguanidine or myoinositol.","PeriodicalId":14040,"journal":{"name":"International journal of experimental diabetes research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80044612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 79

A Note of Thanks to Our Reviewers 感谢我们的审稿人

International journal of experimental diabetes research Pub Date : 2002-01-01 DOI: 10.1080/15604280214486

引用次数: 0

Effect of Aldose Reductase Inhibition on Interleukin-1β-Induced Nitric Oxide (NO) Synthesis in Vascular Tissue 醛糖还原酶抑制对白细胞介素-1β诱导的血管组织一氧化氮合成的影响

International journal of experimental diabetes research Pub Date : 2002-01-01 DOI: 10.1080/15604280212523

J. Morales, J. Dunbar, J. Ram

{"title":"Effect of Aldose Reductase Inhibition on Interleukin-1β-Induced Nitric Oxide (NO) Synthesis in Vascular Tissue","authors":"J. Morales, J. Dunbar, J. Ram","doi":"10.1080/15604280212523","DOIUrl":"https://doi.org/10.1080/15604280212523","url":null,"abstract":"Glucose metabolism via sorbitol pathway has been implicated as a possible contributor to the diabetes-related vascular changes. Nitric oxide plays a major regulatory role in the vascular dilatatory and constricted response. Also it has been observed that diabetes causes vascular changes leading to a decrease in nitric oxide production. Additionally the accumulation of sorbitol is also related to decreased nitric oxide production. In the present study we investigated the effect of normal and high glucose in the presence or absence of both interleukin-1β or an aldose reductase inhibitor on nitric oxide production in rat aortic rings in vitro. Aortic rings from normal male Wistar rats were dissected and incubated for 24 to 48 hrs in the presence of glucose (5.0 mM or 20 mM) or with or without interleukin (20 ng/ml). Other rings were incubated in the above media with the addition of the aldose reductase inhibitor (WAY 121509). Interleukin-1β stimulated the 24 hr nitric oxide production and WAY 121509 decreased it under both low and high glucose culture conditions. The interleukin-1β stimulation was continued for 72 hrs. Nitric oxide production in response to interleukin-1β was greater at all time points when compared to the incubation in media without interleukin-1β. In media containing WAY 121509 the nitric oxide production was decreased. Interleukin-1β stimulated a greater increase in nitric oxide production from aortic rings when incubated in high glucose when compared to normal glucose. The inhibitory effect of aldose reductase inhibition was reversible after 24 hr inhibition under both normal and high glucose conditions. We conclude that high glucose enhances the interleukin- 1β-induced nitric oxide synthesis and the cytokine-induced nitric oxide production was inhibited by aldose reductase inhibition. Nitric oxide production may be linked to redox influences caused by the polyol pathway.","PeriodicalId":14040,"journal":{"name":"International journal of experimental diabetes research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90296629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

The Non-Immune RIP-kb Mouse is a Useful Host for Islet Transplantation, as the Diabetes is Spontaneous, Mild and Predictable 非免疫RIP-kb小鼠是胰岛移植的有效宿主，因为糖尿病是自发性的、轻度的和可预测的

International journal of experimental diabetes research Pub Date : 2002-01-01 DOI: 10.1080/15604280212530

R. Sutherland, Joanne N. Mountford, J. Allison, L. Harrison, A. Lew

引用次数: 10

Renal AT1 Receptor Protein Expression During the Early Stage of Diabetes Mellitus 糖尿病早期肾脏AT1受体蛋白的表达

International journal of experimental diabetes research Pub Date : 2002-01-01 DOI: 10.1080/15604280214483

L. Harrison-Bernard, J. Imig, P. Carmines

引用次数: 42

Streptozocin diabetes elevates all isoforms of TGF-beta in the rat kidney. 链脲佐菌素糖尿病会升高大鼠肾脏中tgf - β的所有亚型。

International journal of experimental diabetes research Pub Date : 2001-01-01 DOI: 10.1155/edr.2001.55

P H Lane, D M Snelling, W J Langer

引用次数: 16

Islet cell antibodies represent autoimmune response against several antigens. 胰岛细胞抗体代表针对几种抗原的自身免疫反应。

International journal of experimental diabetes research Pub Date : 2001-01-01 DOI: 10.1155/edr.2001.85

L Månsson, C Törn, M Landin-Olsson

引用次数: 19