International journal of experimental diabetes research最新文献

Alteration of endothelins: a common pathogenetic mechanism in chronic diabetic complications. 内皮素的改变:慢性糖尿病并发症的常见发病机制。

International journal of experimental diabetes research Pub Date : 2002-10-01 DOI: 10.1080/15604280214939

Subrata Chakrabarti, Zia Ali Khan, Mark Cukiernik, Gen Fukuda, Shali Chen, Suranjana Mukherjee

引用次数: 19

Effect of mild hypoinsulinemia on renal hypertrophy: growth hormone/insulin-like growth factor I system in mild streptozotocin diabetes. 轻度低胰岛素血症对肾肥大的影响:轻度链脲佐菌素糖尿病的生长激素/胰岛素样生长因子I系统。

International journal of experimental diabetes research Pub Date : 2002-10-01 DOI: 10.1080/15604280214937

Mogher Khamaisi, Allan Flyvbjerg, Ziv Haramati, Gadi Raz, Isaiah D Wexler, Itamar Raz

{"title":"Effect of mild hypoinsulinemia on renal hypertrophy: growth hormone/insulin-like growth factor I system in mild streptozotocin diabetes.","authors":"Mogher Khamaisi, Allan Flyvbjerg, Ziv Haramati, Gadi Raz, Isaiah D Wexler, Itamar Raz","doi":"10.1080/15604280214937","DOIUrl":"https://doi.org/10.1080/15604280214937","url":null,"abstract":"The metabolic aberrations associated with diabetes mellitus profoundly alter the growth hormone/insulin-like growth factor I (GH/IGF-I) system. In severe experimental diabetes, serum IGF-I level is reduced, reflecting altered hepatic expression. On the other hand, increased levels of kidney IGF-I have been implicated in the development of diabetic kidney disease. This study aimed to examine the effect of mild experimental diabetes with hypoinsulinemia on both the systemic and renal GH/IGF-I systems in a low-dose streptozotocin (STZ)-induced diabetic rat. Diabetic animals with mild hypoinsulinemia developed renal hyperfiltration within 3 days of diabetes, whereas the renal size increased significantly only between 30 and 48 days of diabetes. Plasma GH levels were unchanged during the entire course of the study, but a decrease in serum IGF-I, IGF-binding protein 3 (IGFBP-3), and IGF-binding protein 4 (IGFBP-4) occurred after 10, 30, and 48 days. Kidney IGF-I and IGF-binding protein 1 (IGFBP-1) mRNA expression increased after 10 and 30 days of diabetes. A significant increase in kidney IGFBP-1/2, IGFBP-3, and IGFBP-4 proteins was seen after 48 days of diabetes. A positive correlations was found between renal growth and insulin/glucose ratio (r=.57), kidney IGF-I (r=.57), IGFBP-1 mRNA (r=.43), IGFBP-1/2 (r=.41), and IGFBP-4 levels (r=.40). These results demonstrate hyperfiltration within 3 days of diabetes and a similar response in the IGF-I system in mildly and severely hypoinsulinemic rats; however, renomegaly develops slower in mildly diabetic rats at least partly due to delayed changes in the renal IGF and IGF BPs.","PeriodicalId":14040,"journal":{"name":"International journal of experimental diabetes research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2002-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15604280214937","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22210754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Effect of acipimox on plasma lipids and glucose/insulin in pregnant rats. 阿昔莫克斯对妊娠大鼠血脂和葡萄糖/胰岛素的影响。

International journal of experimental diabetes research Pub Date : 2002-10-01 DOI: 10.1080/15604280214938

I Sánchez-Vera, B Bonet, M Viana, E Herrera, A Indart

{"title":"Effect of acipimox on plasma lipids and glucose/insulin in pregnant rats.","authors":"I Sánchez-Vera, B Bonet, M Viana, E Herrera, A Indart","doi":"10.1080/15604280214938","DOIUrl":"https://doi.org/10.1080/15604280214938","url":null,"abstract":"To determine how a reduction in maternal hypertriglyceridemia during late pregnancy may affect glucose/insulin relationships, pregnant and virgin rats were orally treated with acipimox, a potent antilipolytic agent. In 20-day pregnant rats receiving 80 mg of acipimox, plasma triglycerides (TG), free fatty acids (FFA), and glycerol decreased more than in virgin rats shortly after the drug (up to 7 hours), when compared with animals treated with distilled water, whereas plasma glucose level was unaffected by the treatment in either group of rats. When acipimox was given every 12 hours from day 17 to day 20 of pregnancy, plasma TG, FFA, and glycerol levels progressively increased, whereas they either decreased or did not change in virgin rats receiving the same treatment, with no effect in plasma glucose levels in either group. Fetal body weight was lower than in controls in 20-day pregnant rats that received acipimox for 3 days. On day 20 of pregnancy, 3 hours after receiving acipimox or distilled water, rats received a 2 g glucose/kg oral load and it was found that the change in plasma glucose was similar in both groups, whereas the increase in plasma insulin was greater in pregnant rats treated with acipimox. However, no difference was found in either variable after the oral glucose load in virgin rats receiving acipimox or distilled water. No differences in plasma glucose levels were found after intravenous (i.v.) administration of insulin in pregnant rats treated or not treated with acipimox. In conclusion, present results show that administration of acipimox during the last days of gestation inhibited lipolysis and decreased fetal weight. Over a short period of time, in pregnant rats, reductions of plasma FFA and TG after acipimox treatment improved the glucose-induced insulin release, but did not seem to have any effect in peripheral insulin resistance.","PeriodicalId":14040,"journal":{"name":"International journal of experimental diabetes research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2002-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15604280214938","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22212094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

C-peptide prevents hippocampal apoptosis in type 1 diabetes. c肽阻止1型糖尿病海马细胞凋亡。

International journal of experimental diabetes research Pub Date : 2002-10-01 DOI: 10.1080/15604280214936

Zhen-guo Li, Weixian Zhang, Anders A F Sima

引用次数: 58

The role of reactive oxygen species in diabetes-induced anomalies in embryos of Cohen diabetic rats. 活性氧在Cohen糖尿病大鼠胚胎中糖尿病诱导异常中的作用。

International journal of experimental diabetes research Pub Date : 2002-10-01 DOI: 10.1080/15604280214933

Sarah W Zangen, Pirhiya Yaffe, Svetlana Shechtman, David H Zangen, Asher Ornoy

{"title":"The role of reactive oxygen species in diabetes-induced anomalies in embryos of Cohen diabetic rats.","authors":"Sarah W Zangen, Pirhiya Yaffe, Svetlana Shechtman, David H Zangen, Asher Ornoy","doi":"10.1080/15604280214933","DOIUrl":"https://doi.org/10.1080/15604280214933","url":null,"abstract":"The role of the antioxidant defense mechanism in diabetes-induced anomalies was studied in the Cohen diabetes-sensitive (CDs) and -resistant (CDr) rats, a genetic model of nutritionally induced type 2 diabetes mellitus. Embryos, 12.5-day-old, of CDs and CDr rats fed regular diet (RD) or a diabetogenic high-sucrose diet (HSD) were monitored for growth retardation and congenital anomalies. Activity of superoxide dismutase (SOD) and catalase-like enzymes and levels of ascorbic acid (AA), uric acid (UA), and dehydroascorbic acid (DHAA) were measured in embryonic homogenates. When fed RD, CDs rats had a decreased rate of pregnancy, and an increased embryonic resorption. CDs embryos were smaller than CDr embryos; 46% were maldeveloped and 7% exhibited neural tube defects (NTDs). When fed HSD, rate of pregnancy was reduced, resorption rate was greatly increased (56%; P < .001), 47.6% of the embryos were retrieved without heart beats, and 27% exhibited NTD. In contrast, all the CDr embryos were normal when fed RD or HSD. Activity of SOD and catalase was not different in embryos of CDs and CDr rats fed RD. When fed HSD, levels of AA were significantly reduced, the ratio DHAA/AA was significantly increased, and SOD activity was not sufficiently increased when compared to embryos of CDr. The reduced fertility of the CDs rats, the growth retardation, and NTD seem to be genetically determined. Maternal hyperglycemia seems to result in environmentally induced embryonic oxidative stress, resulting in further embryonic damage.","PeriodicalId":14040,"journal":{"name":"International journal of experimental diabetes research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2002-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15604280214933","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22212097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 41

Type 2 diabetes--effect of compensatory oversecretion as a reason for beta-cell collapse. 2型糖尿病——代偿性过度分泌作为β细胞衰竭原因的影响。

International journal of experimental diabetes research Pub Date : 2002-07-01 DOI: 10.1080/15604280214276

Valdemar Grill, Anneli Björklund

引用次数: 7

Effect of ethanolic extract of Embelia ribes on dyslipidemia in diabetic rats. 枸杞醇提物对糖尿病大鼠血脂异常的影响。

International journal of experimental diabetes research Pub Date : 2002-07-01 DOI: 10.1080/15604280214278

Uma Bhandari, Raman Kanojia, K K Pillai

{"title":"Effect of ethanolic extract of Embelia ribes on dyslipidemia in diabetic rats.","authors":"Uma Bhandari, Raman Kanojia, K K Pillai","doi":"10.1080/15604280214278","DOIUrl":"https://doi.org/10.1080/15604280214278","url":null,"abstract":"Diabetes mellitus has been treated orally with herbal remedies based on folk medicine since ancient times. Embelia ribes burm (Myrsinaceae), known commonly as vidanga, was used in Ayurveda for its anthelmintic activity. Ayurveda describes vidanga as pungent, causes increase in digestive fire, and cures flatulence and colic. A single study reported the antihyperglycemic activity of decoction of E. ribes in glucose-induced hyperglycemic albino rabbits. In the present study, the lipid-lowering and antioxidant potential of ethanolic extract of E. ribes burm was investigated in streptozotocin (40 mg/kg, IV, single injection)-induced diabetes in rats. Twenty days of orally feeding the extract (200 mg/kg) to diabetic rats resulted in significant (P < 0.01) decrease in blood glucose, serum total cholesterol, and triglycerides, and increase in HDL-cholesterol levels when compared to pathogenic diabetic rats. Further, the extract also lowered the liver and pancreas thiobarbituric acid-reactive substances (TBARSs) values (P < 0.01) when compared to TBARS values of liver and pancreas of pathogenic diabetic rats. The results of test drug were comparable to gliclazide (25 mg/kg, orally), a standard antihyperglycemic agent. This is the first pilot study to provide biochemical evidence of potential of E. ribes in diabetic dyslipidemia.","PeriodicalId":14040,"journal":{"name":"International journal of experimental diabetes research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2002-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15604280214278","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22134647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 75

Protein tyrosine phosphatase activity in insulin-resistant rodent Psammomys obesus. 胰岛素抵抗啮齿动物肥胖的蛋白酪氨酸磷酸酶活性。

International journal of experimental diabetes research Pub Date : 2002-07-01 DOI: 10.1080/15604280214273

Joseph Meyerovitch, Yigal Balta, Ehud Ziv, Joseph Sack, Eleazar Shafrir

{"title":"Protein tyrosine phosphatase activity in insulin-resistant rodent Psammomys obesus.","authors":"Joseph Meyerovitch, Yigal Balta, Ehud Ziv, Joseph Sack, Eleazar Shafrir","doi":"10.1080/15604280214273","DOIUrl":"https://doi.org/10.1080/15604280214273","url":null,"abstract":"Phosphotyrosine phosphatase (PTPase) activity and its regulation by overnight food deprivation were studied in Psammomys obesus (sand rat), a gerbil model of insulin resistance and nutritionally induced diabetes mellitus. PTPase activity was measured using a phosphopeptide substrate containing a sequence identical to that of the major site of insulin receptor (IR) beta-subunit autophosphorylation. The PTPase activity in membrane fractions was 3.5-, 8.3-, and 5.9-fold lower in liver, fat, and skeletal muscle, respectively, compared with corresponding tissues of albino rat. Western blotting of tissue membrane fractions in Psammomys showed lower PTPase and IR than in albino rats. The density of PTPase transmembrane protein band was 5.5-fold lower in liver and 12-fold lower in adipose tissue. Leukocyte antigen receptor (LAR) and IR were determined by specific immunoblotting and protein bands densitometry and were also found to be 6.3-fold lower in the liver and 22-fold lower in the adipose tissue in the hepatic membrane fractions. Liver cytosolic PTPase activity after an overnight food deprivation in the nondiabetic Psammomys rose 3.7-fold compared with postprandial PTPase activity, but it did not change significantly in diabetic fasted animals. Similar fasting-related changes were detected in the activity of PTPase derived from membrane fraction. In conclusion, the above data demonstrate that despite the insulin resistance, Psammomys is characterized by low level of PTPase activities in membrane and cytosolic fractions in all 3 major insulin responsive tissues, as well as in liver. PTPase activity does not rise in activity as a result of insulin resistance and nutritionally induced diabetes.","PeriodicalId":14040,"journal":{"name":"International journal of experimental diabetes research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2002-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15604280214273","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22135237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Fasting decreases the content of D-chiroinositol in human skeletal muscle. 禁食可降低人体骨骼肌中d -肌醇的含量。

International journal of experimental diabetes research Pub Date : 2002-07-01 DOI: 10.1080/15604280214280

Pavel N Shashkin, Laura C Huang, Joseph Larner, George E Vandenhoff, Abram Katz

{"title":"Fasting decreases the content of D-chiroinositol in human skeletal muscle.","authors":"Pavel N Shashkin, Laura C Huang, Joseph Larner, George E Vandenhoff, Abram Katz","doi":"10.1080/15604280214280","DOIUrl":"https://doi.org/10.1080/15604280214280","url":null,"abstract":"Two classes of inositol phosphoglycans have been implicated as second messengers of insulin, one that activates pyruvate dehydrogenase and contains D-chiroinositol, and one that inhibits cyclic AMP-dependent protein kinase and contains myoinositol. We examined the effects of a 3-day fast on muscle contents of inositols in healthy humans. An oral glucose tolerance test was performed and a biopsy was obtained from the quadriceps femoris muscle after an overnight fast and after a 72-hour fast. The 72-hour fast significantly increased plasma glucose (1.5- to 2-fold) and insulin (2- to 4-fold) after glucose ingestion versus the values after the overnight fast, indicating the manifestation of peripheral insulin resistance. The 72-hour fast resulted in an approximately 20% decrease in the muscle content of D-chiroinositol (P < 0.02), but no change in the myoinositol content. These data demonstrate that fasting specifically decreases the muscle content of D-chiroinositol in human muscle and this may contribute to the finding that insulin-mediated activation of pyruvate dehydrogenase is attenuated after short-term starvation.","PeriodicalId":14040,"journal":{"name":"International journal of experimental diabetes research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2002-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15604280214280","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22134648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Endothelin-1-mediated alteration of metallothionein and trace metals in the liver and kidneys of chronically diabetic rats. 内皮素-1介导的慢性糖尿病大鼠肝脏和肾脏中金属硫蛋白和微量金属的改变。

International journal of experimental diabetes research Pub Date : 2002-07-01 DOI: 10.1080/15604280214281

Lu Cai, Shali Chen, Terry Evans, M George Cherian, Subrata Chakrabarti

{"title":"Endothelin-1-mediated alteration of metallothionein and trace metals in the liver and kidneys of chronically diabetic rats.","authors":"Lu Cai, Shali Chen, Terry Evans, M George Cherian, Subrata Chakrabarti","doi":"10.1080/15604280214281","DOIUrl":"https://doi.org/10.1080/15604280214281","url":null,"abstract":"In the present study, the role of endothelin-1 (ET-1) on alterations of hepatic and renal metallothionein (MT) and trace metals (Zn, Cu, and Fe) were investigated in streptozotocin (STZ)-induced diabetic rats. Diabetic rats, age- and sex-matched controls, as well as control and diabetic animals on a dual ETA/ETB receptor blocker, bosentan, were investigated after 6 months of follow-up. MT was measured by cadmium-heme assay. Metals were measured by atomic absorption spectrometer. ET-1 mRNA was analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR) technique. Hepatic and renal ET-1 mRNA was increased in diabetic rats as compared to control rats, along with an increase in both hepatic and renal MT proteins. The increased hepatic MT protein level was associated with decreases in hepatic Cu and Fe, whereas increased renal MT was associated with increases in renal Cu and Fe accumulation. Zn levels were unaltered in both organs in diabetic rats. Bosentan treatment partially prevented the increase in MT levels in both liver and kidney, along with reduced serum creatinine and increased urinary creatinine levels. Further bosentan treatment corrected the increased Cu and Fe levels in the kidney in diabetic rats, but reduced hepatic Cu and Fe levels. No significant effects of bosentan treatment on nondiabetic rats were observed. The data suggest that the possible effects of ET antagonism in diabetes may be mediated via changes in MT and trace metals.","PeriodicalId":14040,"journal":{"name":"International journal of experimental diabetes research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2002-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15604280214281","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22135231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 33