International Dairy Journal最新文献_第10页

Conformational changes of infant formula proteins: effect of whey protein composition and processing step 婴儿配方蛋白构象变化：乳清蛋白组成和加工步骤的影响

IF 3.1 3区农林科学

International Dairy Journal Pub Date : 2025-05-29 DOI: 10.1016/j.idairyj.2025.106312

Aguilar-Morales Mariana, Morales-Camacho Jocksan, Ramírez-Corona Nelly, Jiménez-Munguía María Teresa

{"title":"Conformational changes of infant formula proteins: effect of whey protein composition and processing step","authors":"Aguilar-Morales Mariana, Morales-Camacho Jocksan, Ramírez-Corona Nelly, Jiménez-Munguía María Teresa","doi":"10.1016/j.idairyj.2025.106312","DOIUrl":"10.1016/j.idairyj.2025.106312","url":null,"abstract":"<div><div>This work investigated the cumulative effect of infant milk formula (IMF) processing steps on protein conformation. Model systems were prepared with varying β-Lactoglobulin (β-Lg): α-Lactalbumin (α-Lac): Lactoferrin (Lf): Osteopontin (OPN) ratios: FC (69.6 : 27.9: 2.1 : 0.4), F1 (39.6 : 57.9: 2.1 : 0.4), and F2 (0 : 57.4: 39.9 : 2.7). Protein structural changes were monitored by intrinsic fluorescence, electrophoresis, and available lysine. Whey protein (WP) composition influenced the extent, type, and stoichiometry of protein aggregation. Densitometric analysis revealed that FC, F1, and F2 retained 20.5 ± 2.8 %, 30.2 ± 6.7 %, and 54.8 ± 3.7 % of the native-like whey protein, respectively. Across all formulations, the pasteurization stage held the greatest influence in aggregate formation. Homogenization effects appear to be independent of WP composition. Fluorescence intensity data showed compositional dependence during pasteurization, evaporation, and to a lesser extent, spray drying. Finally, compositional differences between IMFs affected drying-induced lysine glycation, with F2 showing the greatest rate of lactosylation (23.5 %) presumably because of Lf-induced casein dissociation. These findings highlight the interplay between processing steps and IMF composition as drivers of protein conformation.</div></div>","PeriodicalId":13854,"journal":{"name":"International Dairy Journal","volume":"169 ","pages":"Article 106312"},"PeriodicalIF":3.1,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physical properties and stability of milk fat retentate fraction from short-path distillation 短程蒸馏乳脂保留馏分的物理性质及稳定性

IF 3.1 3区农林科学

International Dairy Journal Pub Date : 2025-05-29 DOI: 10.1016/j.idairyj.2025.106310

Sally Samsø Mathiasen , Zheng Guo , Andreas M. Fritzen , Bente Kiens , Maximilian Kleinert , Lars Wiking

{"title":"Physical properties and stability of milk fat retentate fraction from short-path distillation","authors":"Sally Samsø Mathiasen , Zheng Guo , Andreas M. Fritzen , Bente Kiens , Maximilian Kleinert , Lars Wiking","doi":"10.1016/j.idairyj.2025.106310","DOIUrl":"10.1016/j.idairyj.2025.106310","url":null,"abstract":"<div><div>Short-path distillation (SPD) is a method that can be used to separate anhydrous milk fat (AMF) into distillate and retentate fractions, with different fatty acids composition. While our previous work described the potential health benefits of the distillate rich in short- and medium-chain fatty acids, the present study examined the application potential and oxidative stability of the retentate fraction. Retentate were produced and tested at both laboratory and production scale. Overall, retentate was enriched in the fatty acids, C18:0 and C18:1-trans, and reduced in the fatty acids of chain-lengths of C4-14 compared to MF. Differential scanning calorimetry and oscillatory rheology analyses showed early nucleation and network formation for the retentates compared to the MF. Moreover, the retentate had higher solid fat content. Under both high and low supercooling, the hardness during storage for 14 days was similar for MF and retentate. Peroxides formation and color change during storage were unaffected by fat type.</div></div>","PeriodicalId":13854,"journal":{"name":"International Dairy Journal","volume":"169 ","pages":"Article 106310"},"PeriodicalIF":3.1,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144203877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Theoretical analysis of methods to measure homogenisation efficiency, based on centrifugation and droplet size distribution 基于离心和液滴大小分布的均质效率测量方法的理论分析

IF 3.1 3区农林科学

International Dairy Journal Pub Date : 2025-05-28 DOI: 10.1016/j.idairyj.2025.106309

Tomas Skoglund

引用次数: 0

Antibiotics in milk: Validation of analytical methodologies for ensuring food safety 牛奶中的抗生素：确保食品安全的分析方法的验证

IF 3.1 3区农林科学

International Dairy Journal Pub Date : 2025-05-26 DOI: 10.1016/j.idairyj.2025.106308

M. Leite , A. Freitas

{"title":"Antibiotics in milk: Validation of analytical methodologies for ensuring food safety","authors":"M. Leite , A. Freitas","doi":"10.1016/j.idairyj.2025.106308","DOIUrl":"10.1016/j.idairyj.2025.106308","url":null,"abstract":"<div><div>Contamination of milk with antibiotics poses a significant threat to food safety and public health. To address this issue, it is crucial to develop and validate accurate analytical methodologies that can reliably detect and quantify the presence of antibiotics in milk.</div><div>This work aimed to implement an analytical methodology by UHPLC-QTRAP-MS/MS for the determination of 88 antibiotics belonging to ten pharmacological classes in milk. The method showed high selectivity and sensitivity, with linearities above 0.95 obtained through a matrix-matched calibration approach. Decision limit (CCα) values ranged from 1.3 to 239.4 μg kg<sup>−1</sup>. Limits of detection (LOD) and limits of quantification (LOQ) were determined with the highest values of 22.33 μg kg<sup>−1</sup> and 44.85 μg kg<sup>−1</sup>, respectively. Repeatability and reproducibility were below 21.8 and 23.2 %, and recovery ranged between 80.9 and 118.9 %. Application to real samples from local markets demonstrated the presence of macrolides and sulphonamides, though concentrations were below the regulated limits. These results showed the importance of developing and validating new analytical strategies to ensure the safety of dairy products.</div></div>","PeriodicalId":13854,"journal":{"name":"International Dairy Journal","volume":"169 ","pages":"Article 106308"},"PeriodicalIF":3.1,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144190357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of urea adulteration in packaged milk samples using spatially-offset Raman spectroscopy 空间偏移拉曼光谱法检测包装牛奶样品中尿素掺假

IF 3.1 3区农林科学

International Dairy Journal Pub Date : 2025-05-21 DOI: 10.1016/j.idairyj.2025.106307

Anita Dalal , Hemant Krishna , Khan Mohammad Khan , Nitin Kumar , Charu Chourasia , Khageswar Sahu , Srinibas Satapathy , Shovan K. Majumder

{"title":"Detection of urea adulteration in packaged milk samples using spatially-offset Raman spectroscopy","authors":"Anita Dalal , Hemant Krishna , Khan Mohammad Khan , Nitin Kumar , Charu Chourasia , Khageswar Sahu , Srinibas Satapathy , Shovan K. Majumder","doi":"10.1016/j.idairyj.2025.106307","DOIUrl":"10.1016/j.idairyj.2025.106307","url":null,"abstract":"<div><div>Urea adulteration in milk is very rampant worldwide and its consumption can cause serious health hazards. Raman spectroscopy, because of its molecular-specificity, is a powerful analytical tool for biochemical assessment of the urea content in unpackaged milk samples. However, milk is commonly sold in packaged form. Hence, we report the development of a depth sensitive technique “inverse spatially-offset Raman spectroscopy” in combination with a multivariate statistical algorithm for the quantification of the urea adulteration in packaged milk samples. Limit of detection (LOD) and limit of quantification (LOQ) values were found to be 0.22 g/L and 0.67 g/L respectively. Hence, the method suitably detects all urea concentrations above the permissible limit of 0.70 g/L. For example, sample with urea concentration 0.75 g/L, have low Percentage Absolute Error (PAE) of ∼6 %, indicating that the urea concentration can be predicted with a high accuracy of ∼94 % using the developed technique. Further, the prediction accuracy follows an inverse relationship with the thickness of packaging material. The developed technique demonstrates quantification of urea in packaged milk samples, thereby advocating its potential for <em>in-situ</em> monitoring of urea adulteration in a milk production and packaging facility, as well as in the local vending outlets.</div></div>","PeriodicalId":13854,"journal":{"name":"International Dairy Journal","volume":"168 ","pages":"Article 106307"},"PeriodicalIF":3.1,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144139468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation, Identification, biochemical and molecular characterization of potential source of enterotoxigenic strains of Staphylococcus aureus from the bovine raw and mastitis milk 牛乳和乳腺炎乳中金黄色葡萄球菌产肠毒素菌株的分离、鉴定、生化和分子特征分析

IF 3.1 3区农林科学

International Dairy Journal Pub Date : 2025-05-21 DOI: 10.1016/j.idairyj.2025.106306

Sikander Ali , Farzana Nazir , Muhammad Usman Ahmad , Hira Sarfraz , Abid Sarwar , Tariq Aziz , Ashwag Shami , Fahad Al Amsari , Areej A. Alhhazmi , Abeer M. Alghamdi , Fakhria A. Al-Joufi

{"title":"Isolation, Identification, biochemical and molecular characterization of potential source of enterotoxigenic strains of Staphylococcus aureus from the bovine raw and mastitis milk","authors":"Sikander Ali , Farzana Nazir , Muhammad Usman Ahmad , Hira Sarfraz , Abid Sarwar , Tariq Aziz , Ashwag Shami , Fahad Al Amsari , Areej A. Alhhazmi , Abeer M. Alghamdi , Fakhria A. Al-Joufi","doi":"10.1016/j.idairyj.2025.106306","DOIUrl":"10.1016/j.idairyj.2025.106306","url":null,"abstract":"<div><div><em>Staphylococcus aureus</em> is the primary cause of subclinical bovine mastitis, which presents serious problems for the dairy sector. The purpose of this study was to assess the contribution of coagulase-positive <em>S. aureus</em> to subclinical mastitis and investigate any possible risk factors associated with it. One hundred milk samples were aseptically taken from cattle and buffalo in Pakistan's Sheikhupura and District Lahore. The Surf Field Mastitis Test (SFMT) was used to screen the samples, and those that tested positive were processed for bacterial isolation, biochemical characterization, and molecular identification using PCR that targeted the genes for coagulase (coa), mecA, and enterotoxin A. Of the positive samples, 38.6 % (27/70) had no bacterial growth and 61.4 % (43/70) produced staphylococcal isolates on mannitol Agar. All isolates had the coagulase gene (720 bp), and 46.5 % (20/43) of them had <em>S. aureus</em> validated by PCR analysis. Notably, the presence of the mecA gene (630 bp) indicated that 40 % (8/20) of the <em>S. aureus</em> isolates were methicillin resistant. The enterotoxin A gene was not detected in any of the isolates, indicating that <em>S. aureus</em>-induced mastitis is not associated with it. The results show a strong correlation between the occurrence of the disease and possible risk factors, highlighting <em>S. aureus</em> coagulase-positive strains as a key pathogen in subclinical mastitis. This study emphasizes the necessity of focused approaches to better prevent mastitis, manage <em>S. aureus</em> infections, and lessen the effects on the dairy industry.</div></div>","PeriodicalId":13854,"journal":{"name":"International Dairy Journal","volume":"168 ","pages":"Article 106306"},"PeriodicalIF":3.1,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144166531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isomerization and hydrolysis of lactose in arginine solution under microwave heating 微波加热下精氨酸溶液中乳糖的异构化和水解

IF 3.1 3区农林科学

International Dairy Journal Pub Date : 2025-05-20 DOI: 10.1016/j.idairyj.2025.106305

Saowaluck Haosagul , Khwanjai Klinchongkon , Takashi Kobayashi , Yoshiyuki Watanabe , Shuji Adachi , Pramote Khuwijitjaru

引用次数: 0

Brazilian artisan white cheese (queijinho): Characterization of lactic bacteria and their physicoChemical, microbiological, and sensorial properties 巴西手工白奶酪（queijinho）：乳酸菌及其理化、微生物学和感官特性的表征

IF 3.1 3区农林科学

International Dairy Journal Pub Date : 2025-05-15 DOI: 10.1016/j.idairyj.2025.106295

Carolina Krebs de Souza , Tania Maria Costa , Thaís Costa Nihues , Marian Natalie Meisen , Elane Schwinden Prudencio

{"title":"Brazilian artisan white cheese (queijinho): Characterization of lactic bacteria and their physicoChemical, microbiological, and sensorial properties","authors":"Carolina Krebs de Souza , Tania Maria Costa , Thaís Costa Nihues , Marian Natalie Meisen , Elane Schwinden Prudencio","doi":"10.1016/j.idairyj.2025.106295","DOIUrl":"10.1016/j.idairyj.2025.106295","url":null,"abstract":"<div><div><em>Queijinho</em> is an artisanal cheese traditionally made from raw milk and whose know-how was brought to the Blumenau region (Brazil) by German immigrants in the mid-19th century, and to these days it is produced and marketed without control of quality criteria. This study aims to identify lactic acid bacteria and characterize the <em>queijinho</em> based on its physicochemical, microbiological, and sensory properties, contributing to its official registration and production regulation. <em>Queijinho</em> samples donated by three producers were chemically and biologically characterized, and DNA sequencing was used to identify the lactic acid bacteria. <em>Queijinho</em> were classified as skimmed (1.29–9.83 %) and low fat (11.04–14.35 %), based on fat matter in dry extract (FMDE), with protein content ranging from 23.09 ± 0.72 to 18.37 ± 0.86 g/100 g. Thirty-three bacterial genera were identified, particularly <em>Streptococcus thermophilus</em> and <em>Streptococcus salivarius</em>. No harmful bacteria such as <em>Salmonella</em> or <em>Listeria</em> were found, emphasizing good hygienic practices. <em>Queijinho</em> made from pasteurized milk showed higher chewiness (7.850 ± 1.250 N) and acceptance index (47.50 %) in the sensory analysis compared to the samples made from raw milk. The study highlights the significance of maintaining traditional practices in <em>queijinho</em> production, which contribute to its unique sensorial and microbiological properties.</div></div>","PeriodicalId":13854,"journal":{"name":"International Dairy Journal","volume":"168 ","pages":"Article 106295"},"PeriodicalIF":3.1,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144088889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel bacteriophage vB_SauH_SPJ2 against methicillin-resistant Staphylococcus aureus: Characterization and potential application in milk safety 抗耐甲氧西林金黄色葡萄球菌的新型噬菌体vB_SauH_SPJ2：表征及其在牛奶安全中的潜在应用

IF 3.1 3区农林科学

International Dairy Journal Pub Date : 2025-05-14 DOI: 10.1016/j.idairyj.2025.106282

Mengxiao Zhou , Hong Chen , Weiqing Lan , Yong Zhao , Xiaohong Sun

{"title":"Novel bacteriophage vB_SauH_SPJ2 against methicillin-resistant Staphylococcus aureus: Characterization and potential application in milk safety","authors":"Mengxiao Zhou , Hong Chen , Weiqing Lan , Yong Zhao , Xiaohong Sun","doi":"10.1016/j.idairyj.2025.106282","DOIUrl":"10.1016/j.idairyj.2025.106282","url":null,"abstract":"<div><div>Methicillin-resistant <em>Staphylococcus aureus</em> (MRSA), resistant to β-lactam antibiotics, presents critical treatment challenges. Bacteriophages (phages) are increasingly considered an alternative therapy for combating antibiotic-resistant pathogens. This study aimed to isolate and characterize a novel lytic phage targeting MRSA, evaluate its efficacy in biofilm disruption and potential as a biocontrol agent in milk. Phage vB_SauH_SPJ2 was successfully isolated and exhibited a short latent period (15 min) and high burst size (99 PFU/cell). Stability assays showed that SPJ2 remained stable across a broad range of temperatures (4–50 °C) and pH (3–12) conditions. Morphological and genomic analyses revealed that SPJ2 represented a novel species within the <em>Silviavirus</em> genus, belonging to the <em>Twortvirinae</em> subfamily of the <em>Herelleviridae</em> family, lacking resistance genes. SPJ2 significantly disrupted preformed biofilms of MRSA SA008 (59.05 %), MDR <em>S. aureus</em> SA009 (79.15 %), and <em>S. aureus</em> CMCC26003 (56.72 %) after 24 h, while inhibiting biofilm formation by 86.43 %, 44.56 %, and 71.38 % in a concentration-dependent manner, respectively. The SPJ2-encoded lysin may lyse bacteria and degrade extracellular polymeric substances (EPS), thereby inhibiting and removing biofilm synthesis. At a multiplicity of infection (MOI) of 1000, SPJ2 exhibited effective bactericidal activity against <em>S. aureus</em> at 25 °C and 4 °C. Notably, SPJ2 reduced the concentration of <em>S. aureus</em> in milk to undetectable levels within 6 h at 25 °C, demonstrating exceptional biocontrol potential. Statistical significance was analyzed using two-way ANOVA (<em>p</em> < 0.05). These findings highlight the adaptability of SPJ2 to dairy processing environments, efficacy against MRSA, and promise as a synergistic antimicrobial agent for clinical and industrial applications.</div></div>","PeriodicalId":13854,"journal":{"name":"International Dairy Journal","volume":"168 ","pages":"Article 106282"},"PeriodicalIF":3.1,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144088888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of sheep, goat, and cow milk based on non-targeted metabolomics 基于非靶向代谢组学的绵羊、山羊和牛奶的比较分析

IF 3.1 3区农林科学

International Dairy Journal Pub Date : 2025-05-13 DOI: 10.1016/j.idairyj.2025.106292

Jiaxin Liu , Zhongshi Zhu , Yanping Huang , Jianping Qin , Li Wang , Mengyao Wei , Xiaorui Liu , Liang Xu , Hao Yuan , Xiaopeng An , Lei Zhang , Yuxuan Song

{"title":"Comparative analysis of sheep, goat, and cow milk based on non-targeted metabolomics","authors":"Jiaxin Liu , Zhongshi Zhu , Yanping Huang , Jianping Qin , Li Wang , Mengyao Wei , Xiaorui Liu , Liang Xu , Hao Yuan , Xiaopeng An , Lei Zhang , Yuxuan Song","doi":"10.1016/j.idairyj.2025.106292","DOIUrl":"10.1016/j.idairyj.2025.106292","url":null,"abstract":"<div><div>Sheep milk is rich in milk fat, milk protein, vitamins, and minerals, and is also an important source of natural bioactive substances. In recent years, the food and drug functions of sheep milk have been explored, so it is important to understand the specific metabolites in sheep milk in order to study its specific functions. In this study, we performed routine composition analysis of sheep, goat, and cow milk, and non-targeted metabolomics was used to quantify differences in milk metabolites and related metabolic pathways. The contents of fat, non-fat solids, protein, and total solids in sheep milk were significantly higher than those in goat and cow milk (<em>P</em> < 0.05). In total, 178 metabolites were identified in sheep and goat milk, of which 74 were elevated abundance and 104 were lower concentration in sheep milk. In total, 223 metabolites were identified in sheep and cow milk, of which 80 were elevated abundance and 143 were lower concentration in sheep milk. The results indicated that the main differences in metabolites between sheep milk and other milk samples were lipids, lipid analogues, organic acids and their derivatives, organic heterocyclic compounds, nucleotides, nucleotides and their analogues, and were identified through KEGG analysis, mainly enriched in metabolic pathways. This conclusion was consistent with the previous research results of our laboratory that dietary sheep milk can improve lipid metabolism. This study identified the differential metabolites between sheep milk and cow milk as well as goat milk, laying a foundation for subsequent research on the specific bioactive substances and their functions in sheep milk.</div></div>","PeriodicalId":13854,"journal":{"name":"International Dairy Journal","volume":"168 ","pages":"Article 106292"},"PeriodicalIF":3.1,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0