International Journal of Biomedical Science : IJBS最新文献_第5页

Evaluation of Momordica charantia, Boerhaavia diffusa and Cotreatment on Streptozotocin induced Diabetes in Male Wistar Rats 苦瓜、白花布尔哈维亚及共同治疗链脲佐菌素诱导的雄性Wistar大鼠糖尿病的疗效评价

International Journal of Biomedical Science : IJBS Pub Date : 2018-12-15 DOI: 10.59566/ijbs.2018.14066

S. Nwozo, Tochukwu Emmanuel Modeme, Stanley Udogadi Nwawuba

引用次数: 3

Effect of Methanolic Extract of Platycerium angolence on Aluminium Chloride-induced Neurotoxicity in Rats 桔梗甲醇提取物对氯化铝致大鼠神经毒性的影响

International Journal of Biomedical Science : IJBS Pub Date : 2018-12-15 DOI: 10.59566/ijbs.2018.14057

A. Olowoyeye, O. Osukoya, T. Obafemi, J. A. Akinyemi, O. Molehin

{"title":"Effect of Methanolic Extract of Platycerium angolence on Aluminium Chloride-induced Neurotoxicity in Rats","authors":"A. Olowoyeye, O. Osukoya, T. Obafemi, J. A. Akinyemi, O. Molehin","doi":"10.59566/ijbs.2018.14057","DOIUrl":"https://doi.org/10.59566/ijbs.2018.14057","url":null,"abstract":"The aim of this study is to determine the ameliorative effects of methanolic extract of Platycerium angolence (MEPA)on aluminium chloride (AlCl3)-induced neurotoxicity in rats. Rats were randomly separated into five groups containing four rats each. Group 1 serves as the control group and received normal saline, group 2 rats were administered with 100 mg/kg body weight of AlCl3 orally for 28 days to induce neuronal damage, while groups 3 and 4 rats received 250 mg/kg and 500 mg/kg of MEPA for 7days after induction of neuronal damage by AlCl3 for 28 days. Group 5 rats were administered donepezil drug (0.2 mg/kg) which served as reference drug. Behavioural study such as elevated plus maze, Y-maze and open field tests were investigated on the rats. Biochemical assays such as lipid peroxidation, acetylcholinesterase activities, and metal concentration in the whole brain homogenates were estimated using standard procedures. Results revealed a significant (p<0.05) increase in the memory index (MI), lipid peroxidation of animals in the AlCl3 group when compared with the control and the MEPA treated groups. The significant (p<0.05) decrease on lipid peroxidation MEPA was dose dependent. The acetylcholinesterase activities observed in the brain of 250 mg/kg (0.49 μmol acetylcholine/hr/mg protein) group and 500 mg/kg of extract (0.66 μmol acetylcholine/hr/mg protein) has comparative effect with the group treated with the standard drug donepezil when compared with AlCl3 induced group (1.31 μmol acetylcholine/hr/mg protein). This study revealed that both doses of 250 and 500 mg/kg of MEPA has ameliorative potential against AlCl3-induced neurotoxicity in rats but 500 mg/kg of the extract shows better protection.","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76085154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Early Identification of Bacterial Isolates from Positive Blood Culture Bottles using MALDI-TOF MS and Early Antimicrobial Susceptibility Testing on Pediatric Oncology Patients MALDI-TOF质谱法早期鉴定阳性血培养瓶细菌分离株及儿科肿瘤患者早期药敏试验

International Journal of Biomedical Science : IJBS Pub Date : 2018-09-15 DOI: 10.59566/ijbs.2018.14026

M. Elanany, T. Mansour, R. Hassan, Hany Maher

{"title":"Early Identification of Bacterial Isolates from Positive Blood Culture Bottles using MALDI-TOF MS and Early Antimicrobial Susceptibility Testing on Pediatric Oncology Patients","authors":"M. Elanany, T. Mansour, R. Hassan, Hany Maher","doi":"10.59566/ijbs.2018.14026","DOIUrl":"https://doi.org/10.59566/ijbs.2018.14026","url":null,"abstract":"Background: Final identification and susceptibility results of positive blood cultures would take 24 to 48 h by conventional methods. Every hour of early treatment, could be life-saving for septic immunocompromised patients. We conducted a pilot study to determine the earliest timing for performing identification and antimicrobial susceptibility testing of microorganisms isolated, after positive blood culture bottles with the most accurate results. Materials and Methods: Positive blood culture samples were inoculated onto two blood agar plates at 37°C. The growth on one plate was used every one hour repeatedly identification by MALDI-TOF MS (bioMérieux Vitek MS IVD system) and antimicrobial susceptibility testing Vitek 2 system. A total of 600 ID and MIC testing were done (13 Gram positive and 12 Gram negative). Results: Identification of all isolates showed 100% agreement between testing initial 3-hour growth and testing over-night growth. MIC showed 100% agreement when testing at mean hours of 5.6 (SD+/- 2.5) and 9 (SD +/- 2.5) for Gram negative and positive organisms respectively. The total time needed for accurate MIC results from start of positive BC sub-culturing was 16.1 h & 22 h for Gram negative and positive organisms respectively. Conclusion: Identification of causative pathogens using MALDI-TOF MS could be achieved accurately after 3h of sub-culturing. MIC testing can be performed after 5.6 h, 9 h incubation. Applying this methodology provided final accurate rapid results and early treatment of septic patients.","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81848246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The Regulatory/Cytotoxic T Cell Profiles in Blood of Pediatric Kidney-Transplant Recipients with Acute Rejection 急性排斥反应的儿童肾移植受者血液中的调节性/细胞毒性T细胞谱

International Journal of Biomedical Science : IJBS Pub Date : 2018-09-15 DOI: 10.59566/ijbs.2018.14003

F. Fadel, Manal F Elshamaa, Mostafa El-Ahmady, R. Galal, Mona H. Ibrahim, S. Kamel, D. Kandil, D. A. A. Haleem

{"title":"The Regulatory/Cytotoxic T Cell Profiles in Blood of Pediatric Kidney-Transplant Recipients with Acute Rejection","authors":"F. Fadel, Manal F Elshamaa, Mostafa El-Ahmady, R. Galal, Mona H. Ibrahim, S. Kamel, D. Kandil, D. A. A. Haleem","doi":"10.59566/ijbs.2018.14003","DOIUrl":"https://doi.org/10.59566/ijbs.2018.14003","url":null,"abstract":"Background: The equilibrium between regulatory cells and cytotoxic cells may define graft consequence. We investigated the relationship between the expression of main regulatory and cytotoxic markers (i.e., FOXP3 and granzyme B (GZM-B), respectively) and acute rejection (AR) in the peripheral blood of pediatric renal transplant recipients. Methods: In this retrospective study, FOXP3 mRNA expression and serum GZM-B levels in peripheral blood samples from 47 first-time pediatric kidney-transplant recipients were measured, with 17 children classified as possessing AR; whereas the remaining 30 children had functionally stabilized allografts. Results: Levels of the FOXP3 mRNA vs. the expression levels GADPH mRNA (FOXP3 mRNA/GADPH mRNA) were significantly elevated in children with AR than those with stabilized renal allograft (0.48 ± 0.26 vs.0.23 ± 0.18, respectively, P=0.002) Also, serum GZM-B levels in the AR group were elevated than those in the functionally stabilized children (120.07 ± 91.42p g/ml and, 60.16 ± 46.29 pg/ml respectively, P=0.01). ROC curve evidenced that measuring FOXP3 mRNA may have a scope as a decision-taking agent in clinical proceedings to diagnose AR. Measuring peripheral blood FOXP3 mRNA elucidated scope to help in the noninvasive diagnosis of AR. Conclusions: Our results emphasize FOXP3 mRNA as a biomarker for AR in pediatrics. Assessment of regulatory/cytotoxic profiles in the peripheral blood of pediatric renal transplant recipients is a potentially useful tool for patient selection and early detection of rejection. Depending on many variables, such as the method of sample normalization, the technique used, the extent of graft inflammation, the immunosuppression regimen, depletion/ repletion of T-lymphocyte component, the importance of FOXP3 may differ.","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82642216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

To Compare and Evaluate the Bond Strength of Acrylic Denture Teeth with Heat Cure Acrylic Resin and Thermoplastic Denture Base Material after Different Surface Treatments of Acrylic Teeth - An In Vitro Study 热固化树脂与热塑性基托材料对丙烯酸义齿不同表面处理后粘结强度的比较与评价——体外研究

International Journal of Biomedical Science : IJBS Pub Date : 2018-09-15 DOI: 10.59566/ijbs.2018.14020

A. Prasad, Kirti Roopwani, H. S. Mohammed, Vinaya Kundapur, M. Anand, Arpita Dutta

{"title":"To Compare and Evaluate the Bond Strength of Acrylic Denture Teeth with Heat Cure Acrylic Resin and Thermoplastic Denture Base Material after Different Surface Treatments of Acrylic Teeth - An In Vitro Study","authors":"A. Prasad, Kirti Roopwani, H. S. Mohammed, Vinaya Kundapur, M. Anand, Arpita Dutta","doi":"10.59566/ijbs.2018.14020","DOIUrl":"https://doi.org/10.59566/ijbs.2018.14020","url":null,"abstract":"Background: Denture teeth made of acrylic resin are preferred because they chemically bond to denture base material but the problem of debonding is also common with the use of acrylic teeth. Many attempts have been done to improve bond strength of acrylic teeth which involves mechanical and chemical means. With the use of recently introduced thermoplastic denture base materials, the problem of tooth debonding has been increased due to the mechanical nature of the bond between these materials and the acrylic teeth. There is limited literature on bond strength of acrylic teeth and flexible denture materials and the methods to enhance it. The present study evaluates and compares the bond strength between acrylic teeth and heat cure resins and thermoplastic resins after different known denture tooth surface treatment methods, including use of methyl methacrylate; sandblasting and prefabricated vertical groove. Method: A total of 180 maxillary right central incisors were selected and divided into six groups of 30 samples each, according to the surface treatments and denture base material (heat cure or thermoplastic resins) used for processing. The bond strength of all specimens were tested in Universal Testing Machine. The obtained data were subjected to statistical analysis. Result: Results showed that there was statistical significant difference in mean bond strength after various surface treatments and processing of acrylic teeth with either heat cure or flexible denture material. Interpretation & Conclusion: The present findings suggest that vertical groove in the ridge lap area has highest bond strength amongst the groups processed with flexible denture material, and treatment of specimens with methyl methacrylate monomer for 5 sec has highest bond strength amongst all groups.","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85182915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Polypharmacy and Anticholinergic Burden in Hospitalised Older Patients - A Cross Sectional Audit 住院老年患者的综合用药和抗胆碱能负担-横断面审计

International Journal of Biomedical Science : IJBS Pub Date : 2018-09-15 DOI: 10.59566/ijbs.2018.14036

J. Ulley, Sakila Sickander, A. Abdelhafiz

{"title":"Polypharmacy and Anticholinergic Burden in Hospitalised Older Patients - A Cross Sectional Audit","authors":"J. Ulley, Sakila Sickander, A. Abdelhafiz","doi":"10.59566/ijbs.2018.14036","DOIUrl":"https://doi.org/10.59566/ijbs.2018.14036","url":null,"abstract":"Objectives: To investigate the impact of hospital admission on polypharmacy and anticholinergic burden and explore doctors’ awareness of cognitive side effects of anticholinergics use. Methods:A cross sectional retrospective audit of older patients admitted to a care of elderly ward over three months. We have collected patients’ demographic data, number of medications and anticholinergic burden scale on admission compared to on discharge and investigated doctors’ knowledge about polypharmacy by answering a questionnaire. Results: 100 patients were included. Mean number of medications and anticholinergic burden were significantly higher on discharge compared to admission (9.43 v.8.27, p<0.001 and 1.50 v. 1.30, p<0.01, re4spectively). Seven doctors (25%) had no knowledge of any anticholinergic medications at all. Only 16 (57%) doctors were aware of the negative impact of this class of medications on cognitive function and 22 (79%) doctors felt not at all confident in identifying or reducing anticholinergic prescriptions for inpatients. Conclusion:Hospitalisation results in a significant increase in polypharmacy and anticholinergic burden in older people and doctors’ knowledge of the cognitive risks associated with anticholinergic medications in older people is poor.","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76384757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotypic Heterogeneity of the m.3243A>G Variant m.3243A>G变异的表型异质性

International Journal of Biomedical Science : IJBS Pub Date : 2018-09-15 DOI: 10.59566/ijbs.2018.14001

J. Finsterer

引用次数: 0

Effect on Skin Condition by 8-week Ingestion of Standardized Cherry Blossom Flower Extract (Sakura Extract-P) 标准樱花提取物(Sakura Extract- p) 8周对皮肤状况的影响

International Journal of Biomedical Science : IJBS Pub Date : 2018-09-15 DOI: 10.59566/ijbs.2018.14012

Akimasa Matsuyama, Mitsunori Kikuchi, H. Shimoda

{"title":"Effect on Skin Condition by 8-week Ingestion of Standardized Cherry Blossom Flower Extract (Sakura Extract-P)","authors":"Akimasa Matsuyama, Mitsunori Kikuchi, H. Shimoda","doi":"10.59566/ijbs.2018.14012","DOIUrl":"https://doi.org/10.59566/ijbs.2018.14012","url":null,"abstract":"Edible cherry blossom flowers are cultivated in Japan and China. We have previously confirmed that these flowers contain cinnamoyl glucose derivatives and flavonoid glucosides, which reduce advanced glycation end products (AGEs) in skin cells. However, the influence of cherry blossom flower extract (CBE) on the human skin has not been evaluated. Therefore, we conducted a randomized double-blind placebo-controlled study of CBE supplementation in Japanese subjects to examine the effect of standardized CBE (150 mg daily) containing caffeoyl glucose (3 mg). CBE or placebo capsules were administered for 8 weeks to Japanese women aged 30 to 50 years. Skin condition and skin AGEs were evaluated before and after treatment. Photographs of the facial skin were also analyzed and skin symptoms were evaluated on a visual analog scale (VAS). In the CBE group, skin AGEs showed a significant decrease by approximately 7%. In the placebo group, skin elasticity decreased significantly (13%), while no significant change was observed in the CBE group. Furthermore, analysis of photographs revealed a significant decrease of facial pigmented spots and reddish areas in the CBE group versus no significant changes in the placebo group. Thus, CBE was found to reduce skin AGEs, prevent loss of skin elasticity, and decrease facial pigmentation and reddish areas. These results suggest that daily oral intake of CBE might reduce AGE levels in the skin and improve facial pigmentation and reddening.","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80404583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Localized Necrotic Ulceration Gingivitis, in Orthodontic Patient, Treated with Non- Surgical Periodontal Therapy and with Probiotics (L. Reuteri) 正畸患者局部坏死性溃疡性牙龈炎的非手术牙周治疗和益生菌治疗

International Journal of Biomedical Science : IJBS Pub Date : 2018-09-15 DOI: 10.59566/ijbs.2018.14041

Giovanna Mosaico, A. Sinesi, Matteo Fanuli, M. Casalino, Giovanni Mautarelli, C. Casu

{"title":"Localized Necrotic Ulceration Gingivitis, in Orthodontic Patient, Treated with Non- Surgical Periodontal Therapy and with Probiotics (L. Reuteri)","authors":"Giovanna Mosaico, A. Sinesi, Matteo Fanuli, M. Casalino, Giovanni Mautarelli, C. Casu","doi":"10.59566/ijbs.2018.14041","DOIUrl":"https://doi.org/10.59566/ijbs.2018.14041","url":null,"abstract":"Acute Necrotic Ulcerative Gingivitis constitute a group of aggressive semi-reversible periodontal disease usually linked to deep microbiome alterations (Treponema sp., B. melaninogenicus ssp. intermedius and the Fusobacterium sp) and difficult plaque removal. Patients undergoing in fixed orthodontic treatment could have higher risk in periodontal/gum disease development. Therapy for resilient and resistant ANUG is usually represented by pocket debridement and additional systemic therapies (often Metronidazole). In recent years patients resistance to antimicrobial therapies has strongly increased, so probiotic represent a new incoming approach in non surgical periodontal diseases treatment. The aim of this work is to report a case of orthodontic patient with refractory ANUG successfully treated with topical probiotic L. Reuteri. A 16-year-old female with a necrotic ulceration gingivitis reported to our private practice. The patient was submitted to local antiseptics and periodontal debridement for several months without improvement. We had decided to associate a treatment with topical probiotics Lactobacillus reuteri DSM 17938 and lactobacillus reuteri ATCC PTA 5289 to the non-surgical periodontal therapy. The solution of probiotic was injected into the pockets until the spill, for 3-5 minutes and at the end the patient did not rinse the mouth and was advised not to eat and drink for about an hour. The use of topical probiotics in the treatment of gingivitis and ANUG could be considered a valid alternative to conventional treatments for refractory cases in orthodontic patients. Further studies, especially randomized clinical trials, must be performed to confirm this starting result.","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84821663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Condylomata Acuminata of the Urethral Meatus: Successful Combination Therapy 尿道道尖锐湿疣:成功的联合治疗

International Journal of Biomedical Science : IJBS Pub Date : 2018-09-15 DOI: 10.59566/ijbs.2018.14032

R. Khiari, H. Ichaoui, M. Raboudi, B. Kabre, M. A. Bakir, C. Denguir, S. Ghozzi

{"title":"Condylomata Acuminata of the Urethral Meatus: Successful Combination Therapy","authors":"R. Khiari, H. Ichaoui, M. Raboudi, B. Kabre, M. A. Bakir, C. Denguir, S. Ghozzi","doi":"10.59566/ijbs.2018.14032","DOIUrl":"https://doi.org/10.59566/ijbs.2018.14032","url":null,"abstract":"Condylomata acuminata are sexually transmitted papillomatous proliferative lesions caused by the Human Papilloma Virus (HPV) infection. Both sexes can be affected. In male patients, these lesions mostly occur on the glans, frenulum, prepuce and the balanopreputial sulcus. Urethral condyloma is relatively uncommon and it is frequently situated in the distal part of the urethra. We report the case of a 25-year-old male patient, single, whose medical history revealed that he had one unprotected sexual intercourse two years ago. He was presented in our department with a papillary lesion at the urethral meatus that was diagnosed as condyloma acuminatum which was confirmed later by performing a histopathological examination. A local excision of the lesion along with the use of podophyllotoxin as a local adjuvant therapy have led to its disappearance (no local or remote recurrence was seen 8 months later). Through this study, we aimed to discuss the different therapeutic approaches of urethral condylomata and mainly to assess the efficacy of podophyllotoxin as an adjuvant therapy after surgery.","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86610383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1