International Journal of Biomedical Science : IJBS最新文献

{"title":"Polyphenol Family in Cardiovascular disease (CVD): Versatile Therapeutic Outcome","authors":"Emili Manna, S. Maiti","doi":"10.59566/ijbs.2017.13154","DOIUrl":"https://doi.org/10.59566/ijbs.2017.13154","url":null,"abstract":"Epidemiological evidence support that diet rich in polyphenolic compound reduce the risk of cardio vascular disease (CVD). Polyphenols (500-4000 Da) are the main organic constituent presenting with 5-7 phenolic ring with >12 phenolic hydroxyl groups, water soluble plant derived compound. These compounds are also able to generate NO from vascular endothelium. The aim of the mini review was to investigate the role of the most commonly consumed polyphenols in the pathophisiology of the risk of ischemic stroke and CVD. Polyphenol can reduce endothelial dysfunction, in the development of atherosclerosis with the inhibition of the aggregation of platelet and lowering blood pressure. This paper reviews the current advances in polyphenols in food, with emphasising on health aspects on the basis of the published literature, which may provide some guidance for researchers in further investigations and for industries in developing practical health agents.","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87267752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Comparison of the Treatment Outcomes of Transobturator Tape and Anterior Colporraphy in Stress Urinary Incontinence","authors":"Z. Nazari, F. Yazdanpanah, M. Karimi-Zarchi, Negar Ghaffari, Fatemeh Mohajerfar","doi":"10.59566/ijbs.2017.13163","DOIUrl":"https://doi.org/10.59566/ijbs.2017.13163","url":null,"abstract":"Introduction: Stress urinary incontinence (SUI) in women had high prevalence and has a negative impact on their quality of life. Surgery is the most effective way to treat this problem. So, this study designed to compare the efficacy of transobturator tape (TOT) versus anterior colporraphy (APR) in stress incontinence patients. Methods: We designed a prospective cohort study. On base of inclusion and exclusion criteria, we had 65 patients (33 patients in TOT group, 32 patients in APR group) were randomly enrolled in this study. Patients data before surgery, 2 months, 6 months and 18 months after surgery were collected and asked them to complete incontinence quality of life questionnaires (IQOL). We used SPSS 18 for data analysis. Results: Mean surgery time in TOT group was significantly less than APR group (P<0.0001). Quality of life at 6 and 18 months after surgery was significantly better in TOT group (P<0.05). Number of incontinence patients at 2, 6 and 18 months after surgery (P<0.01), incontinence during activity at 18 months after surgery (P=0.031), severity of urinary incontinence at 6 months and 18 months after surgery (P<0.001) were significantly lower in TOT group. Complications after surgery was not different between 2 groups (P=0.78). However, 21 cases in TOT group (63.6%) and 9 cases in APR group (28.1%) complained of pain after surgery (P=0.004). The mean of hospitalization was not statistically significant difference (P=0.54). Finally, 23 patients in TOT group (63.9%) and 13 patients in APR group (36.1%) had urinary incontinence within one last month (OR:3.36, 95%, CL: 1,20-9.36, P=0.018). Furthermore, 25 patients in TOT group (61%) and 16 patients in APR group (39%) had more than 90% improvement in their quality of life (OR: 3.12, 95% CL: 1.08-8.97, P=0.031). Conclusion: The results of this study showed that the TOT techniques more efficient than the APR technique. This technique has more success rate, higher satisfaction of patients and can significantly improve patient's quality of life.","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81124879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro Comparison of Marginal Fit of All Metal, Porcelain Fused to Metal and All Ceramic Crowns","authors":"G. Amarnath, Ishita Jakhanwal, H. Prasad, M. Hilal, Anupama T., Mridul Ayush","doi":"10.59566/ijbs.2017.13147","DOIUrl":"https://doi.org/10.59566/ijbs.2017.13147","url":null,"abstract":"PURPOSE: To compare the marginal fit of all metal, porcelain fused to metal and all ceramic crowns fabricated using different materials and techniques commercially available. MATERIALS AND METHODS: 80 freshly extracted human mandibular first premolars were divided into 8 groups of 10 each which received Nickel-Chromium (Ni-Cr) all metal (AM) crowns, Cobalt-Chromium (Co-Cr) AM crowns, Ni-Cr three-quarter crowns, Co-Cr three quarter crowns, porcelain fused to metal (PFM) crowns with Ni-Cr copings, PFM crowns with Co-Cr copings, pressed all ceramic (AC) crowns and CAD/CAM fabricated AC crowns respectively. Crowns were cemented and specimen were sectioned buccolingually. The marginal gap was evaluated under a stereomicroscope. Lesser marginal gap indicated a better marginal fit. RESULTS: The mean marginal gap was maximum for Group 8 (222.3 μm) and least for Group 1 (85.5 μm). The mean marginal gaps for ‘all metal crowns’ and ‘metal ceramic crowns’ showed significantly lesser marginal gaps (p<0.05) for Ni-Cr Groups than Co-Cr Groups. When only ‘all ceramic crowns’ were compared, significantly lesser marginal gap was found for pressed AC crowns (148.6 μm) than CAD/CAM fabricated AC crowns (222.3 μm). CONCLUSION: Marginal fit of AM crowns were significantly better than PFM crowns and AC crowns. Ni-Cr group always showed better marginal fit than Co-Cr group. A better marginal fit of pressed AC crowns was seen than CAD/CAM fabricated AC crowns. Chamfer finish line showed a significantly better marginal fit than shoulder finish line.","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77801865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of Reversible CD4/CD8+ve T Cell Counts in Patients with HIV Negative Pulmonary Tuberculosis after Effective Treatment with Antitubercular Therapy (ATT)","authors":"G. N. Dhobi, F. Siraj, M. Mir, A. Pandith, I. Qasim, Z. Shah","doi":"10.59566/ijbs.2017.13122","DOIUrl":"https://doi.org/10.59566/ijbs.2017.13122","url":null,"abstract":"Background/Purpose: Tuberculosis (T.B) an infectious disease caused by Mycobacterium tuberculosis is still one of the biggest killers among the infectious diseases. Immunological deficiencies of various magnitudes have been reported in TB which include CD4 + T and CD8 + T cells. The aim of the study was investigating the at the occurrence of CD4+ T cell lymphopenia in patients with active pulmonary tuberculosis and the response of this abnormality to ATT in HIV negative patients. Methods: The study subjects included three groups in which 30 patients were with sputum positive pulmonary tuberculosis without any evidence of HIV infection and second group of10 healthy controls. Third group included10 patients with pulmonary tuberculosis co-infected HIV sero-positivity. Zeil Nelson (ZN) method was used to diagnose pulmonary tuberculosis, ELISA method was adopted to confirm the HIV status and CD4 and CD8 count was done by flow cytometry. Results: The CD­4 + T cell lymphopenia was observed significantly associated (p=0.000) in Non-HIV pulmonary tuberculosis cases as compared to controls (370.87 ± 209.00 cells/μl v/s 673.60 ± 120.30 cells/μl).CD4 + T cell lymphopenia was significantin HIV positive pulmonary TB patients compared to healthy controls (p=0.000). CD4 + T cell lymphopenia was more severe in HIV co-infected pulmonary tuberculosis patients compared to Non-HIV pulmonary TB cases (p=0.029). Conclusion: We conclude that the pulmonary tuberculosis is an important cause of non HIV CD4, CD8 lymphopenia, with reversal of CD4/CD8 ratio, and this is reversible with effective treatment with antitubercular therapy (ATT).","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83938706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Potentials of Edible Freshwater-Snail Bellamya Bengalensis Extract against Arsenic-induced Rat Tissue Damage are Conferred by Antioxidative Mechanism and Attenuation of Pro-Inflammatory Response","authors":"Sk. Sajed Ali, S. Maiti","doi":"10.59566/ijbs.2017.13135","DOIUrl":"https://doi.org/10.59566/ijbs.2017.13135","url":null,"abstract":"Chronic arsenic exposure results in cancer. Some therapeutic agents show inadequate-potency/ side-effects in arsenic-toxicity treatment. The Bellamya bengalensis, an edible snail has long been used by rural people comprised of both ethnic and nonethnic groups as traditional medicine in several health-anomalies/ liver-disorders. In an attempt to investigate the possible protective and therapeutic effect against arsenic induced rat tissue damage are conferred by antioxidative mechanism and attenuation of pro-inflammatory response, the extract of B. bengalensis was tested in arsenic intoxicated rat model. Here, Bellamya bengalensis flesh-extract (BBE, 1 g/kg bw/day for 28days) was tested concomitantly in arsenic-intoxicated (0.6 ppm/kg bw/day for 28days) rat, in in-vitro rat liver slices (in Krebs-ringer buffer for 2 and 4 hours treatment with sodium arsenite alone or with BBE). In the rat, BBE strongly prevented arsenic-induced oxidative/necrotic damages to the intestinal epithelial tissue and liver-tissue/DNA by strengthening the antioxidant-system as shown in Non-protein soluble thiol (NPSH), Superoxide Dismutase(SOD) & catalase results which are clearly reflected in DNA-ladder/comet-assay/histo-architecture results. Arsenic alone decreased catalase and SOD activities in-vivo and in-vitro (H2O2/arsenite redox-stress to dialyzed-concentrated SOD) and also decreased antioxidative signaling molecules i.e. NPSH, serum nitric-oxide (NO) levels. At the same time, arsenic increased the tissue malondialdehyde resulting in DNA-breakage/liver-damage which except NO, were restrained by BBE that constitutes high-level of phosphorus/ascorbate/free-thiols. Moreover, an arsenic-induced increase in pro-inflammatory cytokine TNF-α was restored terminating an acute-phase-reaction. This study, for the first-time, shows the efficiencies of some organism/animal extract in hepatic and intestinal tissue challenged with a high level of arsenic with comparison to the natural level water contamination in West Bengal, India. Our present outcome may be utilized for the development of some protective/therapeutic component against arsenic toxicity from this aquatic organism. Further studies are necessary for more conclusive comments.","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82404676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Dimensional Disparity in Posterior Arch Width Between Opposing Maxillae and Mandibles in Completely Edentulous Patients with Increased Resorption and Edentulous age - An in vivo Study","authors":"C. Shruthi, R. V. Kumar, R. D. Dharmashree, R. Poojya","doi":"10.59566/ijbs.2017.13130","DOIUrl":"https://doi.org/10.59566/ijbs.2017.13130","url":null,"abstract":"Aim: To assess the dimensional disparity in arch width between opposing maxillae and mandibles with edentulous age and increased resorption in edentulous subjects wearing complete denture. Materials and Method: One hundred edentulous subjects wearing complete denture were selected for the study. Maxillary and mandibular casts and a panoramic radiograph were made for each subject. The original height of the mandible before loss of teeth and resorption was predicted by multiplying the distance between the inferior border of the mandible to the lower edge of the mental foramen by a factor of 2.9. The reduction in height of the edentulous mandible was expressed as percentage of the original height of the mandible. The maxillary arch widths immediately anterior to the tuberosities and mandibular arch widths immediately anterior to the retromolar pads were measured. The difference between these two measurements was calculated to determine the discrepancy in posterior arch width. Results: There was no statistically significant difference in the mean values of posterior arch width discrepancy for different groups of edentulous age as well as percentage resorption. The differences in the mean values for amount of resorption with edentulous age were statistically significant. Conclusion: The discrepancy in posterior arch width between opposing maxillae and mandible does not increase progressively with edentulous age and increased reduction in mandibular height. The arch width of the mandible exceeds the arch width of the maxillae in the molar region by an average of 6 to 8 mm after sufficient resorption establishes a definitive alveolar crest. This remains constant regardless of the amount of resorption or edentulous age. The mandibular ridge reduction is found to increase with edentulous age.","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89862578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"To Evaluate Effect of Airborne Particle Abrasion using Different Abrasives Particles and Compare Two Commercial Available Zirconia on Flexural Strength on Heat Treatment.","authors":"Hari A Prasad,&nbsp;Naveed Pasha,&nbsp;Mohammed Hilal,&nbsp;G S Amarnath,&nbsp;Vinaya Kundapur,&nbsp;M Anand,&nbsp;Sumeet Singh","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background and objective: </strong>The popularity of ceramic restorations can be attributed to its life-like appearance, durability and biocompatibility and therefore ceramic restorations have been widely used for anterior and posterior teeth. Ceramic restorations have esthetic and biocompatible advantages but low fracture resistance. Since it has high flexural strength and fracture resistance, yttria-stabilized tetragonal zirconia polycrystal (Y-TZP) is the dental material most commonly used for the core of ceramic crowns and fixed dental prosthesis. In spite of improved mechanical properties, acceptable marginal adaptation and biocompatibility the whitish opacity of zirconia is an obvious esthetic disadvantage. The zirconia framework is often veneered with conventional feldspathic porcelain to achieve a natural appearance. However it is difficult to achieve sufficient bond strength between zirconia and the veneering material. Achieving sufficient bond strength between the veneering ceramic and the zirconia core is a major challenge in the long term clinical success of veneered zirconia restorations. The main objective of this study is to evaluate the effect of different surface treatments on the fracture strength of the two commercially available Zirconia namely Ceramill and ZR-White (AMANNGIRRBACH and UPCERA) respectively.</p><p><strong>Method: </strong>Two commercially available pre-sinteredyttrium stabilized Zirconia blanks (ZR-White and Ceramill) from AMANNGIRRBACH and UPCERA respectively are used to produce the disc shaped specimens of size (15.2 ± 0.03 mm in diameter and 1.2 ± 0.03 mm thick) from each Zirconia blank. All disc shaped specimens are heated at 1200°C in a furnace for 2 hours to form homogenous tetragonal ZrO₂. The dimensions of the specimens are measured with a digital caliper (aerospace). The thickness and diameter of each specimen are calculated as the means of 3 measurements made at random sites. 80 discs from each Zirconia blank are divided into ten groups of 8 specimens each. Heat treatment after airborne-particle abrasion using 50 µm Al₂O₃ particles and 50 µm silica coated Al₂O₃ are applied to the upper and lower surfaces of the specimens. Each specimen is held under a pressure of 30 psi for 15 seconds at a direction perpendicular to the surface and at a distance of 30mm with an airborne particle abrasion device for the specimens in the airborne particle abraded groups. Heat treatments were performed at a starting temperature of 500°C, heating rate of 100°c/ min, ending at a temperature of 1000°C and 15 minutes holding time without vacuum for the specimens in the group 4, 5, 9 and 10. Airborne-particle abrasion mimicking the preparation for cementation was applied to the lower surfaces with 50 µm alumina and silica coated alumina particles for the specimens in the groups 6, 7, 8, 9 and 10. The specimens were cleaned for 15 minutes in an ultrasonic bath co","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5542921/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35284418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Mechanisms and Biological Functions of siRNA.","authors":"Hassan Dana,&nbsp;Ghanbar Mahmoodi Chalbatani,&nbsp;Habibollah Mahmoodzadeh,&nbsp;Rezvan Karimloo,&nbsp;Omid Rezaiean,&nbsp;Amirreza Moradzadeh,&nbsp;Narges Mehmandoost,&nbsp;Fateme Moazzen,&nbsp;Ali Mazraeh,&nbsp;Vahid Marmari,&nbsp;Mohammad Ebrahimi,&nbsp;Mohammad Menati Rashno,&nbsp;Saeid Jan Abadi,&nbsp;Elahe Gharagouzlo","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>One of the most important advances in biology has been the discovery that siRNA (small interfering RNA) is able to regulate the expression of genes, by a phenomenon known as RNAi (RNA interference). The discovery of RNAi, first in plants and <i>Caenorhabditis elegans</i> and later in mammalian cells, led to the emergence of a transformative view in biomedical research. siRNA has gained attention as a potential therapeutic reagent due to its ability to inhibit specific genes in many genetic diseases. siRNAs can be used as tools to study single gene function both in vivo and in-vitro and are an attractive new class of therapeutics, especially against undruggable targets for the treatment of cancer and other diseases. The siRNA delivery systems are categorized as non-viral and viral delivery systems. The non-viral delivery system includes polymers; Lipids; peptides etc. are the widely studied delivery systems for siRNA. Effective pharmacological use of siRNA requires 'carriers' that can deliver the siRNA to its intended site of action. The carriers assemble the siRNA into supramolecular complexes that display functional properties during the delivery process.</p>","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5542916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35284496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lack of Association between Mannose-binding Lectin 2 Codons 54 and 57 Gene Polymorphisms and Cervicovaginal Infections in Mexican Women","authors":"N. Velázquez-Hernández, Marisela Aguilar-Durán, Alma Rosa Pérez-Álamos, S. Estrada-Martínez, J. Salas-Pacheco, L. F. Sánchez-Anguiano, Ada A. Sandoval-Carrillo, Brissia Lazalde-Medina, C. Alvarado-Esquivel","doi":"10.59566/ijbs.2017.13079","DOIUrl":"https://doi.org/10.59566/ijbs.2017.13079","url":null,"abstract":"The mannose-binding lectin (MBL) 2 gene has an important function in the innate immune response and activation of the third pathway of the complement system. Some studies have assessed the association of the MBL2 gene polymorphisms with cervicovaginal infections (CVI); however, there is no information about this association in Mexican women. This study aimed to determine the association between the MBL2 codons 54 and 57 gene polymorphisms with CVI in a sample of Mexican women. Through a cross-sectional study, blood samples and cervicovaginal cultures were obtain from 354 women. MBL2 genotyping was performed by real-time polymerase chain reaction with Taqman probes. Of the 354 women studied, 128 (36.2%) had CVI and 226 (63.8%) were healthy. The frequencies of the C and T variants in codon 54 in women with CVI were 83% and 17%, respectively; whereas the frequencies of these variants in healthy women were 82% and 18%, respectively. The frequencies of variants C/C, C/T, and T/T in women with CVI were 68%, 31%, and 1%, respectively; whereas the frequencies of these variants in healthy women were 68%, 29%, and 3%, respectively. With respect to codon 57, the frequencies of variants C and T were identical in women with CVI and in healthy women (97% and 3%, respectively). The frequencies of variants C/C, C/T, and T/T were identical in women with CVI and in healthy women (94%, 6%, and 0%, respectively). We conclude that MBL2 codons 54 and 57 gene polymorphisms do not associate with CVI in Mexican women.","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85710105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lack of Serological and Molecular Association between <i>Toxoplasma Gondii</i> Exposure and Obesity: A Case-Control Study.","authors":"Cosme Alvarado-Esquivel, Edith Maldonado-Soto, Luis Francisco Sanchez-Anguiano, Jesus Hernandez-Tinoco, Agar Ramos-Nevarez, Sandra Margarita Cerrillo-Soto, Ada Agustina Sandoval-Carrilo, Jose Manuel Salas-Pacheco, Elizabeth Irasema Antuna-Salcido, Sergio Estrada-Martinez, Carlos Alberto Guido-Arreola","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The association between <i>T. gondii</i> infection and obesity has been scantly studied. Through an age-, and gender-matched case-control study, we determined the association of <i>T. gondii</i> infection and obesity using serological and molecular methods. Cases included 203 persons with obesity, and controls included 203 persons without obesity. Participants were tested for the presence of anti-<i>Toxoplasma</i> IgG antibodies using an enzyme-linked immunoassay (EIA). IgG seropositive individuals were further tested for the presence of anti-<i>T. gondii</i> IgM antibodies using an EIA, and <i>T. gondii</i> DNA by polymerase chain reaction (PCR). Anti-<i>T. gondii</i> IgG antibodies were found in 16 (7.9%) of the 203 cases and in 18 (8.9%) of the 203 controls (OR=0.87; 95% CI: 0.43-1.77; <i>P</i>=0.72). One (6.3%) of the 16 anti-<i>T. gondii</i> IgG seropositive cases and 6 (33.3%) of the 18 anti-<i>T. gondii</i> IgG seropositive controls were positive for IgM (<i>P</i>=0.09). Mean body mass index (35.5 ± 4.5) in <i>T. gondii</i> seropositive cases was similar (<i>P</i>=0.57) to that (36.1 ± 4.5) found in <i>T. gondii</i> seronegative cases. Stratification by obesity classes (I, II, and III) did not reveal differences (<i>P</i>>0.05) in seroprevalences (7.8%, 7.9%, and 8.1%, respectively) or high (>150 IU/ml) IgG antibody levels (3.3%, 3.9%, and 2.7%, respectively). PCR was positive in 5 (31.3%) of 16 cases, and in 5 (27.8%) of 18 controls examined (<i>P</i>=1.0). We found no serological or molecular evidence of an association between <i>T. gondii</i> infection and obesity in people attending a public health center in the northern Mexican city of Durango.</p>","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5542918/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35284497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}