International Journal of Dairy Technology最新文献

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":13822,"journal":{"name":"International Journal of Dairy Technology","volume":"79 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148066879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":13822,"journal":{"name":"International Journal of Dairy Technology","volume":"79 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148067109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prebiotics in Brazilian dairy foods: Physicochemical, technological and sensory effects","authors":"Gabriela Secato Rodriguero, Tatiana Colombo Pimentel","doi":"10.1111/1471-0307.70126","DOIUrl":"https://doi.org/10.1111/1471-0307.70126","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The incorporation of prebiotics into dairy foods has traditionally been linked to health-related claims. However, growing evidence shows that these ingredients also exert significant effects on the technological and sensory properties. In Brazil, dairy production combines strong cultural traditions with industrial-scale processing.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aim(s)</h3>\u0000 \u0000 <p>This review critically analysed the effects of incorporating prebiotics on the physicochemical, technological and sensory properties of Brazilian dairy foods, with an emphasis on traditional products.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A structured literature search was conducted in major scientific databases to identify peer-reviewed studies reporting the application of prebiotics in Brazilian dairy foods. Inclusion criteria focused on studies that assessed key product properties, including physicochemical characteristics, processing behaviour and sensory attributes in traditional products.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Major Findings</h3>\u0000 \u0000 <p>Prebiotic addition was associated with technological and sensory benefits, although the magnitude and direction of the effects were matrix-dependent. In weakly structured systems, such as Minas Frescal cheese and whey- or milk-based beverages, prebiotics increased viscosity, physicochemical uniformity and product stability. In more concentrated matrices, such as requeijão cremoso and doce de leite, the addition of prebiotics primarily altered the internal structure, resulting in improved spreadability, creaminess and overall sensory balance. Fat content, prebiotic type and concentration, and processing conditions emerged as key modulators of these responses, influencing moisture retention and textural behaviour. Prebiotic concentrations ranged from approximately 0.49% to 6.0%, reflecting distinct formulation objectives rather than standardised dose–response strategies. Several studies reported increased consumer acceptance, reduced undesirable attributes such as excessive greasiness, and improvements in aroma and overall sensory balance; however, systematic dose–response evaluations and comprehensive sensory analyses remain limited in the literature.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Scientific or Industrial Implications</h3>\u0000 \u0000 <p>This review indicates the technological potential of prebiotics beyond health positioning, provides guidance on ingredient selection and formulation strategies for Brazilian dair","PeriodicalId":13822,"journal":{"name":"International Journal of Dairy Technology","volume":"79 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1471-0307.70126","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148067107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and phenotype characterisation of persistent Escherichia coli in dairy products from Midwest Brazil","authors":"Adriane de Souza Nunes Sikorski,&nbsp;Adelino Cunha Neto,&nbsp;Elisana Cecilia Nunes Bueno,&nbsp;Márcio Gonçalo de Lima,&nbsp;Samera Rafaela Bruzaroski,&nbsp;Vinicius Silva Castro,&nbsp;Maxsueli Machado,&nbsp;Yuri Duarte Porto,&nbsp;Elsa Helena Walter Santana,&nbsp;Eduardo Eustáquio de Souza Figueiredo,&nbsp;Ricardo César Tavares Carvalho","doi":"10.1111/1471-0307.70127","DOIUrl":"https://doi.org/10.1111/1471-0307.70127","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Biofilm-forming <i>Escherichia coli</i>, carrying genes for the transmissible locus of stress tolerance (tLST) and pathogenicity genes, pose a risk to public health and dairy production.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To evaluate the phenotypic and molecular characteristics of <i>E. coli</i> strains isolated from two segments of the dairy supply chain: inspected milk and raw milk and dairy products without official inspection.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Classical methods were used to identify and characterise 77 <i>E. coli</i> strains isolated from 98 milk and dairy product samples in Midwest Brazil regarding biofilm formation, curli protein and motility. In addition, molecular methods were used to investigate pathogenicity genes (<i>eaeA</i>, <i>ehxA</i>, <i>stx1</i>, and <i>stx</i>2), heat resistance, and stress resistance (tLST and <i>rpoS</i>).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Major Findings</h3>\u0000 \u0000 <p>Through the <i>yaiO</i> gene, 75 strains were identified as <i>E. coli</i>, 39 from refrigeration tanks and 36 from raw milk and dairy products. Of these strains, 26.67% (20/75) were positive for the <i>eaeA</i> gene and 4% (3/75) for the <i>ehxA</i> gene. Regarding the genes to biofilm formation (<i>rpoS</i>) and stress tolerance (tLST), amplification was detected in nine and six strains, respectively. The 20 strains carrying the <i>eaeA</i> gene were classified as enteropathogenic <i>E. coli</i> (EPEC). All strains were phenotypically positive in the motility test; 98.67% were classified to produce weak, moderate, strong, very strong and extremely strong biofilm intensities. In addition, curli protein expression was detected in 49.33% of the <i>E. coli</i> strains.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Scientific Implications</h3>\u0000 \u0000 <p>The ability of <i>E. coli</i> strains isolated from products originating from two distinct segments of the dairy sector to produce biofilms, carry genes for resistance to thermal stress and pathogenicity, suggests strong persistence in these environments, as well as indicating a potential risk to public health.</p>\u0000 </section>\u0000 </div>","PeriodicalId":13822,"journal":{"name":"International Journal of Dairy Technology","volume":"79 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1471-0307.70127","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148067108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial resistance and virulence profiles of Enterococcus spp. isolated from raw milk of different animal species in Türkiye","authors":"Seyda Sahin,&nbsp;Mahmut Niyazi Mogulkoc,&nbsp;Cemil Kurekci","doi":"10.1111/1471-0307.70125","DOIUrl":"https://doi.org/10.1111/1471-0307.70125","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Milk derived from various animal species is widely consumed, necessitating careful evaluation of its microbiological safety. Enterococci are recognised as important reservoirs of antimicrobial resistance (AMR) and virulence determinants, representing a potential concern within the One Health framework.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p>This study was conducted to assess the prevalence, antimicrobial susceptibility profiles, virulence factors and resistance genes of <i>Enterococcus</i> spp. isolated from milk samples (<i>n</i> = 250) collected from several animal species (cows, sheep, goats and water buffalo) in Türkiye.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Isolation was performed using selective <i>Enterococcus agar</i> with and without vancomycin. Identification relied on conventional microbiological techniques and polymerase chain reaction (PCR). Antimicrobial susceptibility testing was conducted using disc diffusion, while vancomycin susceptibility was evaluated using the <i>E</i>-test. Species identification, along with detection of resistance genes (glycopeptide, macrolide, tetracycline, aminoglycoside) and virulence genes, was carried out by PCR.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 250 milk samples, 151 (60.4%) <i>Enterococcus</i> spp. strains were recovered, with the isolated species <i>E. faecium</i> (<i>n</i> = 59), <i>E. faecalis</i> (<i>n</i> = 44) and others (<i>n</i> = 48). Vancomycin resistance was detected in 6.6% of the isolates. The resistance rates observed in the isolates were as follows: kanamycin (79.5%), penicillin (40.4%), rifampicin (37.7%), tetracycline (11.3%), quinupristin-dalfopristin (6%), erythromycin (5.3%) and linezolid (2.6%). Upon examination of virulence genes, <i>Enterococcus</i> isolates (<i>n</i> = 11) were positive for <i>gelE</i>, eight for <i>esp</i>, four for <i>asa1</i> and one for <i>hlyA</i>. The <i>cylA</i> gene was absent in all isolates. While resistance to tetracycline was shown to be primarily associated with tetM, erythromycin-resistant strains were found to be positive for the <i>mefA/E</i> gene.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Scientific Implications</h3>\u0000 \u0000 <p>This study provides comparative data on species-level differences in the occurrence and AMR patterns of <i>Enterococcus</i> spp. isolated from raw milk, based on phenotypic testing and selected gene detection across ruminant sources.</p>\u0000 </section>\u0000 </div>","PeriodicalId":13822,"journal":{"name":"International Journal of Dairy Technology","volume":"79 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148068427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":13822,"journal":{"name":"International Journal of Dairy Technology","volume":"79 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148066886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The underestimated factors affecting biofilm formation and microbial food safety in dairy processing environments","authors":"Marlenne X Atta-Delgado,&nbsp;Tahirou Sogore,&nbsp;Run-run Zhang,&nbsp;Xinyu Liao,&nbsp;Jinsong Feng,&nbsp;Donghong Liu,&nbsp;Jian He,&nbsp;Xiaoyan Pei,&nbsp;Juhee Ahn,&nbsp;Tian Ding","doi":"10.1111/1471-0307.70124","DOIUrl":"https://doi.org/10.1111/1471-0307.70124","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Bacterial biofilms are a persistent and mechanistically complex source of contamination in dairy processing environments, posing serious food safety risks due to their protection of pathogens and resistance to conventional sanitation methods.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aim(s)</h3>\u0000 \u0000 <p>This review aimed to examine the influence of dairy composition and processing conditions on biofilm formation, extending previous composition-focussed analyses by integrating microbial interactions, surface conditioning effects and the often insufficiently integrated role of commensal bacteria in biofilm persistence and pathogen protection.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A comprehensive synthesis of peer-reviewed literature was conducted through systematic searches of Scopus, Web of Science, PubMed and Google Scholar, focussing on biofilm formation in dairy systems, dairy matrix effects, microbial interactions and sanitation strategies.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Major Findings</h3>\u0000 \u0000 <p>The nutrient-rich composition of dairy products (proteins, fats, lactose and minerals) contributes to biofilm development by enhancing microbial adhesion and extracellular polymeric substance (EPS) production, although the role of individual components, particularly lactose, is highly species- and context-dependent. Processing conditions (moisture levels, temperature and fluid dynamics) and surface properties (material, roughness and hydrophobicity) further influence biofilm dynamics. Commensal bacteria, increasingly recognised as active contributors to biofilm ecology, participate in biofilm stabilisation, facilitate pathogen survival and exhibit resistance to sanitisers. Advances in detection methods, including molecular and imaging tools, have improved biofilm monitoring. Integrated control strategies, such as enzymatic cleaners, bacteriophages, nonthermal technologies, hygienic design and staff training, are essential for effective biofilm management.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Scientific or Industrial Implications</h3>\u0000 \u0000 <p>The findings underscore the need for a multidisciplinary approach combining microbiology, food engineering and industrial practice to mitigate biofilm-related risks. This review provides actionable insights for dairy processors to enhance biofilm detection, control and prevention, thereby supporting global food safety standards and improving product quality across the supply chain.</p>\u0000 </section>\u0000 </div>","PeriodicalId":13822,"journal":{"name":"International Journal of Dairy Technology","volume":"79 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148068434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathway-linked milk and serum metabolites predict cheesemaking traits in Holstein cows: Glycerophospholipid, nicotinamide, amino acid and purine signatures","authors":"Ahmed Emam","doi":"10.1111/1471-0307.70116","DOIUrl":"https://doi.org/10.1111/1471-0307.70116","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Background/Context/Rationale</h3>\u0000 \u0000 <p>Milk yield and cheesemaking traits arise from metabolic networks governing nutrient partitioning and mammary biosynthesis, yet biochemical predictors remain underexplored in Holsteins.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aim(s)</h3>\u0000 \u0000 <p>To identify milk/serum metabolites and pathways predicting cheese yield, rennet coagulation and recovery via untargeted LC–MS/MS metabolomics.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Untargeted LC–MS/MS profiling was conducted on milk and serum from 40 multiparous Holstein cows, stratified by yield [high-yielding (HY) ≥35 kg/day, <i>n</i> = 20; low-yielding (LY) ≤25 kg/day, <i>n</i> = 20]. The principal component analysis /PERMANOVA separated the profiles (<i>P</i> &lt; 0.001), while partial least squares discriminant analysis predicted cheese yield (RY = 0.78, <i>Q</i> = 0.71), recovery (RY = 0.82) and protein retention (RY = 0.79); biomarkers were prioritised by VIP &gt;1.5, followed by pathway enrichment testing.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Major Findings</h3>\u0000 \u0000 <p>Glycerophospholipids [lysoPC(16:0), SM(d18:1/16:0)] dominated PC1 (42.3% variance); nicotinamide, glycine and xanthine elevated in HY (<i>P</i> &lt; 0.01). Pathways included glycerophospholipid (<i>P</i> = 0.002), purine (<i>P</i> = 0.003) and amino acid metabolism. Pilot cheesemaking validated correlations (<i>r</i> = 0.81–0.89, <i>P</i> &lt; 0.01).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Scientific/Industrial Implications</h3>\u0000 \u0000 <p>This study establishes precision dairy biomarkers for early milk selection, nutritional optimisation and cheesemaking efficiency in Holstein herds.</p>\u0000 </section>\u0000 </div>","PeriodicalId":13822,"journal":{"name":"International Journal of Dairy Technology","volume":"79 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148068428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":13822,"journal":{"name":"International Journal of Dairy Technology","volume":"79 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148065169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":13822,"journal":{"name":"International Journal of Dairy Technology","volume":"79 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148130185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}