International archives of allergy and applied immunology最新文献_第9页

Smooth muscle mechanics: implications for airway hyperresponsiveness. 平滑肌力学:对气道高反应性的影响。

International archives of allergy and applied immunology Pub Date : 1991-01-01 DOI: 10.1159/000235387

R R Schellenberg, P D Pare, J Hards, K Ishida

{"title":"Smooth muscle mechanics: implications for airway hyperresponsiveness.","authors":"R R Schellenberg, P D Pare, J Hards, K Ishida","doi":"10.1159/000235387","DOIUrl":"https://doi.org/10.1159/000235387","url":null,"abstract":"Human bronchial smooth muscle preparations from 10 freshly dissected pneumonectomy samples were evaluated for their mechanical characteristics and compared with results obtained for similar samples obtained from porcine trachea. Length-tension relationships of in vitro smooth muscle were evaluated for passive stretching as well as active isometric force generation and isotonic shortening using electrical field stimulation. At the length (Lmax) producing maximal force (Pmax) resting tension was very high (60.0 +/- 8.8% Pmax) compared with porcine trachealis (5.2 +/- 2.3% Pmax). Maximum shortening was 25.0 +/- 9.0% at a length of 75% Lmax with suboptimal shortening occurring at Lmax (12.0 +/- 3.4%) for the human bronchus, whereas optimal shortening of porcine trachealis (71.4 +/- 3.6%) occurred at Lmax. Morphometric evaluation revealed threefold less muscle per cross-sectional area of tissue for human (8.7 +/- 1.5%) versus porcine (24.8 +/- 1.9%) preparations. We conclude that the high passive tension and the decreased maximum shortening are produced by a relatively large load which must be overcome for the muscle to shorten, presumably provided by the greater connective tissue elastic component present in the airway. We suggest that a decrease in airway wall elastance would increase smooth muscle shortening, thereby leading to excessive responses to contractile agonists as seen in vivo in asthma.","PeriodicalId":13810,"journal":{"name":"International archives of allergy and applied immunology","volume":"94 1-4","pages":"291-2"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000235387","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13095673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Allergic inflammation and its pharmacological modulation in asthma. 过敏性炎症及其在哮喘中的药理调节。

International archives of allergy and applied immunology Pub Date : 1991-01-01 DOI: 10.1159/000235363

S T Holgate, R Djukanović, J Wilson, W Roche, K Britten, P H Howarth

{"title":"Allergic inflammation and its pharmacological modulation in asthma.","authors":"S T Holgate, R Djukanović, J Wilson, W Roche, K Britten, P H Howarth","doi":"10.1159/000235363","DOIUrl":"https://doi.org/10.1159/000235363","url":null,"abstract":"While most asthma occurs in association with atopy, the relationship of this to clinical expression of the disease is not clearly understood. Allergen provocation causes an immediate bronchoconstriction (early asthmatic reaction) due to the release of mast-cell-derived histamine, prostaglandin D2 and leukotriene C4. The late reaction and attendent increase in bronchial responsiveness are associated with eosinophil influx, activation and mediator secretion, resulting in mucosal swelling in addition to smooth muscle contraction. Endobronchial biopsy and broncho-alveolar lavage have provided compelling evidence that both mast cells and eosinophils contribute to disordered airway function in 'clinical' asthma and that these cells are under the control of T lymphocytes. Topical corticosteroids which produce beneficial clinical effects probably do so by inhibiting those factors that maintain mast cell and eosinophil populations and their enhanced activation. The most likely contenders for these regulatory functions are the cytokines, particularly interleukin-3, -4 and -5.","PeriodicalId":13810,"journal":{"name":"International archives of allergy and applied immunology","volume":"94 1-4","pages":"210-7"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000235363","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13095788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Immunoregulation of endometrial and jejunal epithelia sensitized by infection. 感染致敏的子宫内膜和空肠上皮的免疫调节。

International archives of allergy and applied immunology Pub Date : 1991-01-01 DOI: 10.1159/000235427

G A Castro, Y Harari

{"title":"Immunoregulation of endometrial and jejunal epithelia sensitized by infection.","authors":"G A Castro, Y Harari","doi":"10.1159/000235427","DOIUrl":"https://doi.org/10.1159/000235427","url":null,"abstract":"The hypothesis was tested that the uterus of the rat orally infected with the parasite Trichinella spiralis becomes hypersensitized and that subsequent antigenic challenge affects functions in the endometrial epithelium. Results of experiments comparing the immunological responsiveness of isolated rat uterus with that of the jejunum supports our hypothesis. Antigenic challenge of uterus mounted in Ussing-type chambers causes an elevation in transuterine short circuit current (Isc) of 6.4 +/- 0.8 microA/cm2. The transduction of the antigenic signal to elicit the electrophysiological response involves 5-hydroxytryptamine (5-HT) working through a nerve-independent pathway. The antigen-stimulated rise in Isc peaks approximately 3 min after challenge. The uterine response is blocked by diisothiocyanostilbene-2,2'-disulfonic acid, an inhibitor of bicarbonate-chloride exchange. The antigen-evoked change in jejunal Isc is biphasic, peaking at 1.5 and approximately 4.0 min after challenge, and is about 10-fold greater in magnitude than the Isc in the uterus. The transductive pathway in the jejunum involves 5-HT, histamine and prostaglandin acting partly through intrinsic nerves. The jejunal response to antigen is inhibited by diphenylamine-2-carboxylate, a chloride channel blocker. Changes in net ion transport which are primed by infection and evoked by antigen are apparently triggered by local anaphylaxis in both the uterus and jejunum.","PeriodicalId":13810,"journal":{"name":"International archives of allergy and applied immunology","volume":"95 2-3","pages":"184-90"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000235427","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13097402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

T cells and asthma. II. Regulation of the eosinophilia of asthma by T cell cytokines. T细胞和哮喘。2T细胞因子对哮喘嗜酸性粒细胞的调节。

International archives of allergy and applied immunology Pub Date : 1991-01-01

C Walker, J C Virchow, P L Bruijnzeel, K Blaser

引用次数: 0

IgE-mediated inhalant allergy against human corticotropin-releasing hormone. ige介导的人促肾上腺皮质激素释放激素吸入性过敏。

International archives of allergy and applied immunology Pub Date : 1991-01-01 DOI: 10.1159/000235467

J Kleine-Tebbe, R Wahl, K Dierks, G Kunkel

引用次数: 0

Ability of polymorphonuclear leukocytes to generate active oxygen species in children with bronchial asthma. Use of chemiluminescence probes with a Cypridina luciferin analog and luminol. 支气管哮喘患儿多形核白细胞产生活性氧的能力。化学发光探针与荧光素类似物和发光氨的使用。

International archives of allergy and applied immunology Pub Date : 1991-01-01 DOI: 10.1159/000235448

M Kato, M Nakano, A Morikawa, H Kimura, M Shigeta, T Kuroume

{"title":"Ability of polymorphonuclear leukocytes to generate active oxygen species in children with bronchial asthma. Use of chemiluminescence probes with a Cypridina luciferin analog and luminol.","authors":"M Kato, M Nakano, A Morikawa, H Kimura, M Shigeta, T Kuroume","doi":"10.1159/000235448","DOIUrl":"https://doi.org/10.1159/000235448","url":null,"abstract":"Airway inflammation with polymorphonuclear leukocytes (PMN) may play an important role in bronchial hyperresponsiveness (BHR). PMN generate superoxide anion (O2-) and other oxygen radicals that can damage lung tissue. We investigated the ability of peripheral PMN of children with bronchial asthma and control subjects to generate O2- and other active oxygen species using a 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin++ +-3-one, a highly sensitive and specific chemiluminescence (CL) probe for O2-, and luminol-dependent CL. The ability of PMN of subjects with asthma to generate O2- and other active oxygen species was significantly greater than that of PMN of control subjects when stimulated with opsonized zymosan (OZ), phorbol myristate acetate or N-formyl-methionyl-leucyl-phenylalanine. Furthermore, in the same asthmatic children, the generation of O2- and other active oxygen species was significantly higher with attacks than without attacks when PMN were stimulated with OZ. We also demonstrated that O2- generation correlated with the degree of BHR to inhaled histamine. These results suggest that PMN of asthmatic children, especially those with attacks, generate more active oxygen species than that of control subjects and that airway inflammation caused by O2- may be closely related to BHR in subjects with bronchial asthma.","PeriodicalId":13810,"journal":{"name":"International archives of allergy and applied immunology","volume":"95 1","pages":"17-22"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000235448","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12821399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 31

Nasal inflammatory mediator release in ragweed allergic rhinitis: correlation with cellular influx into nasal secretions. 豚草变应性鼻炎的鼻腔炎症介质释放:与细胞流入鼻腔分泌物的相关性。

International archives of allergy and applied immunology Pub Date : 1991-01-01 DOI: 10.1159/000235500

J W Georgitis, B D Stone, G Gottschlich

{"title":"Nasal inflammatory mediator release in ragweed allergic rhinitis: correlation with cellular influx into nasal secretions.","authors":"J W Georgitis, B D Stone, G Gottschlich","doi":"10.1159/000235500","DOIUrl":"https://doi.org/10.1159/000235500","url":null,"abstract":"Lipid-derived mediators are found in nasal secretions during the early and late phase of allergic responses. To explore this early response further, concentrations of inflammatory mediators were measured along with characterization of specific cell influx during dose-dependent ragweed challenges. Ten allergic rhinitis subjects underwent two unilateral nasal lavages using 3-fold concentrations of short ragweed antigen. Low doses of ragweed (0.016-0.114 units Amb a I) did not provoke cell influx (1 of 18 challenges), whereas moderate doses (0.432-1.3 units Amb a I) induced cell influxes in 7 of 18 and at high doses in 8 of 17 challenges (3.39-11.7 units Amb a I). The differential of the cellular influx was greater than 50% neutrophils in 7 challenges; greater than 50% eosinophils in 3, and a mixed pattern in 6. There was a significant association between the dose of antigen and the level of prostaglandin D2 (PGD2), leukotrienes (LTs) C4, D4 and E4. Challenges with an eosinophilic influx tended to be associated with higher concentrations of mediators than neutrophilic influxes. Similar to the immediate skin response, the early allergic response in the nose demonstrated a cell influx with release of PGD2, LTsC4, D4 and E4. Nasal cellular inflammation therefore can occur within minutes of allergen exposure.","PeriodicalId":13810,"journal":{"name":"International archives of allergy and applied immunology","volume":"96 3","pages":"231-7"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000235500","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12832536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Increased numbers of Th2-like CD4+ T cells in target organs and in the allergen-specific repertoire of allergic patients. Possible role of IL-4 produced by non-T cells. 靶器官和过敏原特异性库中th2样CD4+ T细胞数量增加。非t细胞产生的IL-4可能的作用。

International archives of allergy and applied immunology Pub Date : 1991-01-01 DOI: 10.1159/000235344

S Romagnani, E Maggi, P Parronchi, D Macchia, M P Piccinni, M Ricci

{"title":"Increased numbers of Th2-like CD4+ T cells in target organs and in the allergen-specific repertoire of allergic patients. Possible role of IL-4 produced by non-T cells.","authors":"S Romagnani, E Maggi, P Parronchi, D Macchia, M P Piccinni, M Ricci","doi":"10.1159/000235344","DOIUrl":"https://doi.org/10.1159/000235344","url":null,"abstract":"Phytohemagglutinin (PHA)-induced human T cell clones (TCC) derived from conjunctival flogistic tissues of 3 patients with vernal conjunctivitis produced unusually high amounts of interleukin-4 (IL-4) and no, or limited amounts of, gamma-interferon (IFN-gamma). Allergen (Dermatophagoides pteronyssinus or Lolium perenne group I)-specific TCC derived from peripheral blood of two atopic donors produced significantly higher amounts of IL-4 and significantly lower amounts of IFN-gamma than TCC specific for bacterial antigens (tetanus toxoid and PPD) contemporarily established from the same donors. These data provide evidence for a compartimentalization of Th2-like helper T cells in target organs and in the allergen-specific T cell repertoire of allergic patients. Non-B, non-T bone marrow cells could produce IL-4, but not IL-2 or IFN-gamma, in response to cross-linkage of Fc epsilon type I receptors. These cells may further contribute to the maintenance and amplification of allergic inflammation.","PeriodicalId":13810,"journal":{"name":"International archives of allergy and applied immunology","volume":"94 1-4","pages":"133-6"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000235344","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12847000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 45

Interleukin-5 enhances the in vitro adhesion of human eosinophils, but not neutrophils, in a leucocyte integrin (CD11/18)-dependent manner. 白细胞介素-5以白细胞整合素(CD11/18)依赖的方式增强人嗜酸性粒细胞的体外粘附，而不是中性粒细胞。

International archives of allergy and applied immunology Pub Date : 1991-01-01 DOI: 10.1159/000235355

G M Walsh, A J Wardlaw, A Hartnell, C J Sanderson, A B Kay

{"title":"Interleukin-5 enhances the in vitro adhesion of human eosinophils, but not neutrophils, in a leucocyte integrin (CD11/18)-dependent manner.","authors":"G M Walsh, A J Wardlaw, A Hartnell, C J Sanderson, A B Kay","doi":"10.1159/000235355","DOIUrl":"https://doi.org/10.1159/000235355","url":null,"abstract":"Interleukin (IL-5) was found to enhance the adhesion of eosinophils, but not neutrophils, to both microvascular and large vein endothelial cells in a dose-dependent manner. Granulocyte/macrophage-colony-stimulating factor (GM-CSF) and platelet-activating factor (PAF) enhanced both eosinophil and neutrophil adhesion. Significant increases in eosinophil CR3 expression, but not LFA-1, were observed following pre-incubation with PAF, IL-3, IL-5 or GM-CSF. Neutrophil CR3 expression was increased significantly by pre-incubation with PAF or GM-CSF, but not IL-3 or IL-5. Enhanced adhesion to human microvascular endothelial cells (HMVEC) or human umbilical vein endothelial cells (HUVEC) was inhibited by (ranked in order of potency) anti-CR3 alpha = common beta-chain greater than LFA-1 alpha. Anti-p150,95 alpha had no measurable effect. Basal expression of eosinophil CR3 with monoclonal antibody inhibited IL-5-induced eosinophil hyperadherence to HUVEC in a manner almost identical to inhibition in the presence of excess anti-CR3. Thus, a conformational or affinity change in adhesion receptors following activation seems more important than a simple increase in numbers. No inhibition of unstimulated eosinophil adhesion to HMVEC or HUVEC by CD11/18 monoclonal antibody was observed. These findings demonstrate that IL-5 enhances eosinophil, but not neutrophil, adherence reactions, by a mechanism dependent, at least in part, on the CD11/18 family of adhesion glycoproteins.","PeriodicalId":13810,"journal":{"name":"International archives of allergy and applied immunology","volume":"94 1-4","pages":"174-8"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000235355","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12847001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 55

Effects of cetirizine on human eosinophil and neutrophil activation in vitro. 西替利嗪对体外人嗜酸性粒细胞和中性粒细胞活化的影响。

International archives of allergy and applied immunology Pub Date : 1991-01-01 DOI: 10.1159/000235422

G M Walsh, R Moqbel, A Hartnell, A B Kay

引用次数: 40