International Journal of Current Microbiology and Applied Sciences最新文献

{"title":"Oral Cholera Vaccine (Shanchol) is Effective to Prevent Cholera: A Message Learnt from a Post Vaccination Diarrhoea Surveillance Study","authors":"Hemant Kumar Khuntia, Bhagyalaxmi Biswal, Shantanu Kumar Kar, B. Dwibedi, Jyostnamayee Sabat, Prasanta Kumar Bramha, S. Pati, A. S. Kerketta","doi":"10.20546/ijcmas.2023.1207.013","DOIUrl":"https://doi.org/10.20546/ijcmas.2023.1207.013","url":null,"abstract":"A diarrhea surveillance study was conducted after a mass vaccination with a new Oral cholera vaccine Shanchol, in India. During the study period between 2011-2013, a total of 4050 vaccinated, non-vaccinated resident and traveler diarrhoea patients were examined for different etiologial agents. Among the pathogens, toxigenic E. coli (15.15%), V. cholera (3.38%) and rotavirus (29.12%) were found to be main organisms. The isolation frequencies of V. cholera in vaccinated diarrhea patients, in non-vaccinated diarrhea patients in vaccinated area, in non-vaccinated diarrhea patients in neighboring vaccinated area and in non-vaccinated traveler diarrhea patients from other states were 0.72% (4 of 550), 2.3% (12 of 517), 3.7% (109 of 2885) and 12.2% (12 of 98) respectively. The isolation of V. cholerae from vaccinated diarrhea patients is significantly less than the non-vaccinated traveler diarrhea patients which indicate that reduction of cholera among vaccinated people might be due to immunization with Shanchol. Development of herd immunity may be responsible for less isolation of V. cholerae among non-vaccinated residents in vaccinated area compared to non-vaccinated traveler diarrhea patients. It is suggested to vaccinate the vulnerable population using Shanchol to prevent cholera infection in Puri, in other parts of the country and globe.","PeriodicalId":13777,"journal":{"name":"International Journal of Current Microbiology and Applied Sciences","volume":"69 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139361067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sequence Characterization of Anti Tumorigenic (IL12B) and Pro Tumorigenic (IL17A) Ovine Interleukins for an Immune Approach to Cancer Immunotherapy","authors":"Kritika Gaur, J. Anuradha, Vivek Pratap Singh, A. S. Meena, Rajiv Kumar","doi":"10.20546/ijcmas.2023.1207.005","DOIUrl":"https://doi.org/10.20546/ijcmas.2023.1207.005","url":null,"abstract":"Cancer is a complex illness characterized by uncontrolled cell growth and the spread of abnormal cells throughout the body. Immunotherapy is one of the cancer treatment modalities gained prominence in recent years. Immunotherapy is a type of bio-therapy that works by retraining the patient's immune system to recognize cancer, boosting selectivity, and decreasing side effects. Immune modulation is a complex process involving several cell types and factors. High-dose of IL-12 provides long-term responses in a subset of patients with metastatic melanoma and renal cell carcinoma. IL-17 has been identified as a frequent cytokine in the tumour microenvironment that can have dual roles in cancer growth and tumour eradication. In present study, ovine Interleukin 12B and 17A genes were amplified, cloned, and in silico analyzed for its potential in cancer treatment.","PeriodicalId":13777,"journal":{"name":"International Journal of Current Microbiology and Applied Sciences","volume":"97 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139361068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Root and Leaf Phenomenons of C3 Plants Infected with Vesicular Arbuscula Mycorrhizal","authors":"Muhammad Akhsan Akib, Syat rawati, R. Prayudyaningsih","doi":"10.20546/ijcmas.2023.1207.019","DOIUrl":"https://doi.org/10.20546/ijcmas.2023.1207.019","url":null,"abstract":"The utilization of VAM in plants generally uses powder dosage forms and not yet used dosage forms of sachet and tablet. The research aimed to examine the phenomenon of the roots and leaves of C3 plants infected with VAM in different dosage forms at the screen house scale. The research was conducted at the Agrotechnology Laboratory and Screen House, Faculty of Agriculture, Animal Husbandry and Fisheries, Muhammadiyah University of Parepare, using factorial design. The first factor was the VAM dosage forms, namely powder/control, sachet, and tablet dosage forms. While, other factors were the C3 plant species, namely Puero (Pueraria javanica)/control, mung bean (Vigna radiata), and red spinach (Amaranthus tricolor). The parameter observations on the percentage of infected roots, root volume, leaf area, and stomata density. Data were analyzed using variance analysis. The results showed that the VAM dosage form had a significant effect on all parameters observed with various root and leaf phenomena so VAM in a sachet and tablet dosage form can be recommended as a new dosage form for VAM application in the field.","PeriodicalId":13777,"journal":{"name":"International Journal of Current Microbiology and Applied Sciences","volume":"57 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139360719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adoption of Scientific Fish Farming of Pengba (Osteobrama belangeri) by the Fish Farmers in the Valleys of Manipur","authors":"Oinam Naresh Khuman, Y. J. Singh, A. Sarkar, A. B. Patel, P. Pal, H. Bharati, K. Singh, C. Pegu, K. Borah","doi":"10.20546/ijcmas.2023.1207.004","DOIUrl":"https://doi.org/10.20546/ijcmas.2023.1207.004","url":null,"abstract":"The present study examined the extent of adoption of scientific fish farming of Pengba (Osteobrama belangeri) by the fish farmers in the valleys of Manipur. Three districts viz., Bishnupur, Imphal West and Thoubal were purposively selected for the study. A sample size of 80 respondents was selected after consultation with Department of Fisheries, Government of Manipur and with the village key informants. Pre tested and structured interview schedule was administered for data collection. The study found that only 40.06 per cent of the respondents had fully adopted the scientific fish farming of Pengba. The variables such as experience in fisheries activities, knowledge level, family size, annual income and scientific orientation had significant and positive relationship with the extent of adoption of scientific fish farming of Pengba. This study also revealed that knowledge level, scientific orientation, annual income and family size were the best predictors to the total explained variation of 52.50 per cent to the extent of adoption of scientific fish farming of Pengba.","PeriodicalId":13777,"journal":{"name":"International Journal of Current Microbiology and Applied Sciences","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139360931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Helicobacter pylori Virulence Genes cagE, iceA and oipA in Stool Samples from Burkina Faso","authors":"T. Compaoré, Yasmine Astrid Sana, Sylvie Zida, N. I. Compaore, L. Traoré, O. Ouédraogo, Abdou Azaque Zoure, S. Soubeiga, D. Kambire, Kalifou Traoré, T. Sagna, Florencia Wendkuuni Djigma, Henri Gautier Ouedraogo, J. Simporé","doi":"10.20546/ijcmas.2023.1207.006","DOIUrl":"https://doi.org/10.20546/ijcmas.2023.1207.006","url":null,"abstract":"Helicobacter pylori infection leads to gastritis that can evolve into severe forms of ulceration and malignant transformation. Its diagnosis is most often invasive. Our study aim was to characterize by a non-invasive method, Helicobacter pylori virulence genes iceA, oipA, and cagE in stool samples from Burkina Faso. Methods: This cross-sectional study took place from September 2020 to February 2021 and involved 250 patients. Each patient provided a stool sample, which was used to perform an Helicobacter pylori antigenic detection test and molecular analysis by polymerase chain reaction (PCR) to detect its virulence genes cagE, iceA, and oipA. Results: The results showed a Helicobacter pylori infection rate of 89.6%. The virulence genes cagE, iceA1, iceA2, and oipA frequencies were 75.4%, 3.12%, 3.12%, and1.78% respectively. Our bacterial population had only one iceA genotype. The cagE gene was significantly linked with H. pylori infection (p-value=0.001). We also found a significant association between the iceA2 gene and a risk factor, with a (p-Value= 0.01). Conclusion: Our study is one of the first characterizing cagE, iceA1, iceA2, and oipA virulence genes of Helicobacter pylori in Burkina Faso. It suggests that virulence genes can be tested in stool as an on-invasive method.","PeriodicalId":13777,"journal":{"name":"International Journal of Current Microbiology and Applied Sciences","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139360772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Inter-Relationship and Principal Component Analysis for Determination of the Selection Criteria in Mulberry Genotypes","authors":"Suresh Kotigi, Manj appa, Y. Harijan, Nongthombam Lemba Luwang, Deepika Umesh Kumar, Chikkamudde Marulasiddappa Kishor Kumar","doi":"10.20546/ijcmas.2023.1207.010","DOIUrl":"https://doi.org/10.20546/ijcmas.2023.1207.010","url":null,"abstract":"The identification of superior clones in mulberry breeding programs requires the knowledge on genetics of economic traits and efficient tools for selection. A field experiment was carried to evaluate two pseudo F2 populations of CSRS-1 × V1 and Kajli OPH × V1 for leaf yield and quality attributes during Kharif 2018. The objective of this study was to determine the interrelationship among different traits and to identify superior clones by principal component analysis. One way analysis of variance revealed that the progenies of both populations are highly significant for all quantitative traits studied indicating opportunity for genetic selection. Higher mean, standardized range and genotypic coefficient of variances was observed for fresh leaf weight, leaf area, primary shoots, total shoots length and leaf yield per plant. High to moderate heritability in broad sense and genetic advance in percent of mean was recorded for all the characters except for chlorophyll content index, specific leaf area, leaf moisture content and leaves per meter shoot. Phenotypic correlation coefficients and path analysis revealed that selection based on higher fresh leaf weight, primary shoots and total shoots length would be effective for increasing the leaf yield of mulberry. Platykurtic and positively skewed distribution noticed in these traits indicated the involvement of a several minor genes and dominance based complementary gene interaction suggests that rapid genetic gain under mild selection. PCA identified four principal components (PC) and explained 72 to 77% of total variation present in both the population. PC1, PC2, and PC3 were related to leaf productivity, quality and leaf size, respectively. PCA based index identified superior clones CV2, CV109, KV40, KV103 and KV169 may be further evaluated to develop improved mulberry varieties for subtropical sericulture.","PeriodicalId":13777,"journal":{"name":"International Journal of Current Microbiology and Applied Sciences","volume":"78 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139360823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemistry and Therapeutic Applications of Eucalyptus – An Overview","authors":"S. Suganya, S. Vijayanand, K. Vanmathiselvi","doi":"10.20546/ijcmas.2023.1207.022","DOIUrl":"https://doi.org/10.20546/ijcmas.2023.1207.022","url":null,"abstract":"Eucalyptus globulus, which is commonly seen, has immense potential for medicinal purposes. The blue gum, scientifically called Eucalyptus globulus, is a type of plant that belongs to the Myrtaceae family. It consists of abundant amounts of phytochemical constituents such as flavonoids, alkaloids, tannin, and propanoids which can be found in the leaves, stem, bark and roots of the plant. Multiple researchers have reported the existence of different attributes in these properties, including anti-inflammatory, antibacterial, antiseptic, astringent, anti-diabetic, anti-oxidative, antiviral, antitumor, antihistaminic, anticancer cytochrome p450 inhibitor, and hepatoprotective effects. This review article thoroughly examines different phytochemicals present in Eucalyptus globulusand highlights the various therapeutic uses of these compounds.","PeriodicalId":13777,"journal":{"name":"International Journal of Current Microbiology and Applied Sciences","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139361112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Organic Starter Solution on Vase Life of Chrysanthemum (Dendranthema grandiflora Tzvelev)","authors":"Ankita Chouhan, T. Tirkey, G. Sharma","doi":"10.20546/ijcmas.2023.1207.011","DOIUrl":"https://doi.org/10.20546/ijcmas.2023.1207.011","url":null,"abstract":"The present investigation entitled “Impact of organic starter solution on vase life of chrysanthemum (Dendranthema grandiflora Tzvelev)” was carried out at Department of Floriculture and Landscape Architecture, College of Agriculture, Indira Gandhi Krishi Vishwavidyalaya, Raipur during 2018-19. The experiment was laid out in Completely Randomized Design with thirteen treatments and three replications consisting of organic starter solution alone and in combination with PSB and Trichoderma. The purpose of the experiment was to evaluate the result of applying organic starter solution in plants, comparing it to inorganic starter solutions to evaluate the vase life of chrysanthemum grown in potted condition. Studies showed organic starter solutions significantly improved the freshness of flower over control. Maximum vase life (19.67 days) was recorded at application of treatment T8 (Goatery slurry + PSB) which was significantly superior over all the other treatments including control. Minimum vase life (17.20 days) was recorded in the treatment T13 (RDF 2% inorganic liquid19:19:19) taken as control.","PeriodicalId":13777,"journal":{"name":"International Journal of Current Microbiology and Applied Sciences","volume":"165 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139361155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Constraints as Perceived by the Subject Matter Specialists of Selected Krishi Vigyan Kendras of Assam","authors":"Nabanil Talukdar, Pabitra Kumar Das, Avijeet Borah","doi":"10.20546/ijcmas.2023.1207.008","DOIUrl":"https://doi.org/10.20546/ijcmas.2023.1207.008","url":null,"abstract":"KVK plays a pivotal role in streamlining and offering an advantageous route to the increase of the agricultural zone of various regions in the country. It would be helpful to operate smoothly for the KVKs if the problems faced by the SMSs are properly addressed as they are the grass-root level functionaries of the organization. For that purpose the present study was conducted in 23 KVKs which are under Assam Agricultural University in the State of Assam. Out of 123 SMSs, 80 SMSs from 21 KVKs constituted the sample of respondents for the study. A questionnaire with structured and open-ended questions was created, and dichotomous responses were produced. The findings revealed that majority (62.5%) of the respondents belonged to the age category of 33 - 44 years, followed by 21.25 per cent who belonged to 45 - 52 years category and 16.25 per cent who belonged to 29 - 32 years category. Majority (71.25%) of the respondents were M.Sc degree holders while others (28.75%) were Ph.D degree holders. Majority (58.75%) of the respondents had 4 to 7 years of service experience. For determining the constraints six (6) different dimensions were considered in the study namely technological constraints, administrative constraints, and socio-economic constraints, input or other material constraints, personal constraints and other miscellaneous constraints. It is observed that 58.75 per cent of the SMSs expressed low economic status and social obstacles of farmers followed by overload of reports, meetings and activities apart from KVK mandated activities (57.5%), lack of proper infrastructure for better execution (46.25%), additional workload affecting social and personal life (42.5%), difficulty in disseminating complex technology (41.25%) and poor infrastructure maintenance (16.25%) as major constraints under each dimension respectively. Apart from that majority of the respondents mentioned about lack of women farmer-friendly technology, lack of location specific technology, shortage of transportation facility, shortage of manpower, lack of financial ability of farmers to accept agriculture as a business, high input cost, less opportunity of HRD training for SMS as constraints related to their field of activities.","PeriodicalId":13777,"journal":{"name":"International Journal of Current Microbiology and Applied Sciences","volume":"372 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139360711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Standardization of Age of Kokum Seedling (Garcinia indica Choisy) for Transferring in Polybag","authors":"Prashant Pralhadrao Debaje, M. Gawankar, H. P. Naik, P. C. Haldavanakar, G. Khandakar, P. Haldankar","doi":"10.20546/ijcmas.2023.1207.012","DOIUrl":"https://doi.org/10.20546/ijcmas.2023.1207.012","url":null,"abstract":"The demand of kokum graft, as well as, seedlings, is more in konkan region and Maharashtra, Goa region. To fulfill the demand, as well as for healthy grafts the experiment was laid out at AICRP Oil Palm, College of Horticulture, Mulde along with 12 treatments and 2 replications. Fresh clean seeds of ripe kokum fruits were sown on nursery bed and after germination they were used for transferring in 6x8’’poly bags having 4x6’’Soil +FYM +Rice husk+2gm VAM (2:1:1). As per T1 treatment some seeds sown directly in poly bag, some seedling transfer as per treatments after sprouting T2, 10 days T3, 20 days T4, 30 days T5, 40 days T6, 50 days T7, 60 days T8, 70 days T9, 80 days T10, 90 days T11, 100 days seedling T12. Observation were recorded at 30,180,270 and 360 days after transferring in poly bags. The result recorded that T3 10 days seedling recorded significantly minimum days to attain the graftable stage i.e., 173 days after transferring along with highest percentage of survival 98.17 %.","PeriodicalId":13777,"journal":{"name":"International Journal of Current Microbiology and Applied Sciences","volume":"186 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139360802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}