International Hepatology Communications最新文献_第9页

National strategies for viral hepatitis B, C and hepatocellular carcinoma in Taiwan 台湾地区病毒性乙型肝炎、丙型肝炎及肝细胞癌的国家策略

International Hepatology Communications Pub Date : 1996-05-01 DOI: 10.1016/S0928-4346(96)82010-X

Juei-Low Sung

引用次数: 1

The role of intracellular calcium in the production of Superoxide anion by Kupffer cells stimulated by lipopolysaccharide and the efficacy of prostaglandin E1 胞内钙在脂多糖刺激Kupffer细胞产生超氧阴离子中的作用及前列腺素E1的作用

International Hepatology Communications Pub Date : 1996-04-01 DOI: 10.1016/0928-4346(96)00264-2

Haruki Kurosawa , Hiroshi Shimada, Akira Nakano, Shiho Natori, Yuichi Fujii

{"title":"The role of intracellular calcium in the production of Superoxide anion by Kupffer cells stimulated by lipopolysaccharide and the efficacy of prostaglandin E1","authors":"Haruki Kurosawa , Hiroshi Shimada, Akira Nakano, Shiho Natori, Yuichi Fujii","doi":"10.1016/0928-4346(96)00264-2","DOIUrl":"10.1016/0928-4346(96)00264-2","url":null,"abstract":"<div><p>Kupffer cells stimulated by lipopolysaccharide (LPS) produce Superoxide anion and cause hepatocellular injury. Intracellular calcium has been noted to work as a second messenger in a variety of pathophysiological mechanisms. The aims of this study were to investigate the effect of LPS stimulation of Kupffer cells on intracellular calcium concentrations and the production of Superoxide anion, and determine the effect of prostaglandin E<sub>1</sub> (PGE<sub>1</sub>) on intracellular calcium and the Superoxide anion production following stimulation with LPS. Kupffer cells were isolated from male Wistar rats. The intracellular calcium level and the production of Superoxide anion were measured using a fluorescence computed microscope (Mu-1000, Inter Dec) and a Luminescence Reader (BLR-103, Aloka). After LPS stimulation, the intracellular calcium level of Kupffer cells increased and pretreatment with PGE<sub>1</sub> reduced this increase. Pretreatment with PGE<sub>1</sub> and removal of extracellular calcium decreased the production of Superoxide anion from Kupffer cells stimulated by LPS. We conclude that an increase of intracellular calcium affects production of Superoxide anion from Kupffer cells stimulated by LPS. Pretreatment with PGE<sub>1</sub> reduces the production of Superoxide anion by preventing this rise in intracellular calcium.</p></div>","PeriodicalId":13746,"journal":{"name":"International Hepatology Communications","volume":"4 6","pages":"Pages 326-333"},"PeriodicalIF":0.0,"publicationDate":"1996-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0928-4346(96)00264-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78521777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Immunohistochemical observation of Fas in the human liver: light and electron microscopic observation by immuno-peroxidase method 人肝脏Fas免疫组化观察:免疫过氧化物酶法光镜及电镜观察

International Hepatology Communications Pub Date : 1996-04-01 DOI: 10.1016/0928-4346(96)00265-4

Masahiro Takatani , Gotaro Yamada , Hirofumi Tsugeno , Fumitoshi Kishi , Toshihiko Doi , Shin Yonehara , Takao Tsuji

{"title":"Immunohistochemical observation of Fas in the human liver: light and electron microscopic observation by immuno-peroxidase method","authors":"Masahiro Takatani , Gotaro Yamada , Hirofumi Tsugeno , Fumitoshi Kishi , Toshihiko Doi , Shin Yonehara , Takao Tsuji","doi":"10.1016/0928-4346(96)00265-4","DOIUrl":"10.1016/0928-4346(96)00265-4","url":null,"abstract":"<div><p>The expression of Fas was examined in liver specimens from 18 patients with various liver diseases (8 type B chronic hepatitis, 7 type C chronic hepatitis, 2 autoimmune hepatitis and one fatty liver) and normal liver specimens obtained from 2 patients under surgical treatments for metastatic liver cancer, by light and electron microscopy using the indirect peroxidase-labelled antibody method. Fas was detected on the surface of hepatocytes in all cases. By immunoelectron microscopy, dense reaction products were observed on the plasma membrane facing both the Disse's and intercellular spaces, as well as in some membranes of the endoplasmic reticulum. In patients with severe or moderate chronic liver inflammation, strongly Fas-positive hepatocytes were distributed diffusely throughout the lobules. On the other hand, in patients with minimal hepatic inflammation, Fas expression was weak, and localized mainly in hepatocytes of the centrilobular area. These findings confirm the localization of Fas on the plasma membrane of hepatocytes, and suggest that Fas expression may increase with increasing degree of intrahepatic inflammation.</p></div>","PeriodicalId":13746,"journal":{"name":"International Hepatology Communications","volume":"4 6","pages":"Pages 334-342"},"PeriodicalIF":0.0,"publicationDate":"1996-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0928-4346(96)00265-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75914594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Little expression of proto-oncogene Bcl-2 in tumourous cells of hepatocellular carcinoma with chronic hepatitis C virus infection 原癌基因Bcl-2在慢性丙型肝炎病毒感染的肝癌肿瘤细胞中表达少

International Hepatology Communications Pub Date : 1996-04-01 DOI: 10.1016/0928-4346(96)00263-0

Yoshiki Ito , Norio Hayashi , Yutaka Sasaki , Masayoshi Horimoto , Shigeo Wada , Yuji Tanaka , Taizo Hijioka , Kunio Suzuki , Hideyuki Fusamoto , Jiro Fujimoto , Eizo Okamoto , Takenobu Kamada

{"title":"Little expression of proto-oncogene Bcl-2 in tumourous cells of hepatocellular carcinoma with chronic hepatitis C virus infection","authors":"Yoshiki Ito , Norio Hayashi , Yutaka Sasaki , Masayoshi Horimoto , Shigeo Wada , Yuji Tanaka , Taizo Hijioka , Kunio Suzuki , Hideyuki Fusamoto , Jiro Fujimoto , Eizo Okamoto , Takenobu Kamada","doi":"10.1016/0928-4346(96)00263-0","DOIUrl":"10.1016/0928-4346(96)00263-0","url":null,"abstract":"<div><p>The proto-oncogene bcl-2, which was first found overexpressed in human follicular lymphoma, is now considered to inhibit apoptotic cell death, resulting in greater cell susceptibility to genetic alteration. Thus, this gene might cause hyperplasia or occasionally lead to a more malignant phase, i.e. carcinoma. Overexpression of this gene has been reported in several tumors. However, little has been clarified about its contribution to hepatocarcinogenesis. In this study, using immunoblotting, immunohistochemical and in situ hybridization techniques, we examined the expression of the bcl-2 protein and messenger RNA in patients having hepatocellular carcinoma with chronic hepatitis C virus infection. Immunoblot analysis indicated no significant differences in the amounts of bcl-2 protein between tumorous and nontumorous lesions. Furthermore, immunohistochemistry as well as in situ hybridization technique demonstrated that only lymphocytes infiltrating the tissues and tumors were bcl-2 positive. These findings suggest that the proto-oncogene bcl-2 may make no or little contribution, if any, to hepatocarcinogenesis.</p></div>","PeriodicalId":13746,"journal":{"name":"International Hepatology Communications","volume":"4 6","pages":"Pages 316-325"},"PeriodicalIF":0.0,"publicationDate":"1996-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0928-4346(96)00263-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81304720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

The effect of oxidative injury on hepatocellular influx of leucine in the rat 氧化损伤对大鼠肝细胞亮氨酸内流的影响

International Hepatology Communications Pub Date : 1996-04-01 DOI: 10.1016/0928-4346(96)00266-6

David G. Le Couteur , Laurent P. Rivory, Susan M. Pond

引用次数: 2

Gene therapy of hepatoma by intratumoral injection with thymidine kinase gene and ganciclovir administration 胸苷激酶基因瘤内注射加更昔洛韦治疗肝癌的研究

International Hepatology Communications Pub Date : 1996-04-01 DOI: 10.1016/0928-4346(95)00261-8

Yoshiyasu Kaneko , Ayumi Tsukamoto

引用次数: 0

Collagen content of liver biopsy specimens in patients with chronic hepatitis 慢性肝炎患者肝活检标本胶原蛋白含量

International Hepatology Communications Pub Date : 1996-04-01 DOI: 10.1016/0928-4346(96)00262-9

Hitomi Nakabayashi , Seigo Takamatsu , Hiroyuki Tsujii , Yasuyuki Okamoto , Hiroshi Nakano , Eiji Yamada , Kunio Ichijima

引用次数: 11

High prevalence of hepatitis C virus genotype la in Japanese hemophiliacs 乙型肝炎病毒la基因型在日本血友病患者中的高流行率

International Hepatology Communications Pub Date : 1996-04-01 DOI: 10.1016/0928-4346(96)00260-5

Takeaki Suou , Toshio Kawatani , Kenichi Nishikawa , Juichi Ueki , Hiroaki Sawada , Shinji Ooi , Minoru Yamada , Hiroyuki Sasaki , Hironaka Kawasaki

引用次数: 2

Analysis of interferon regulatory factor 1 and 2 gene expression in human hepatoma cell lines 干扰素调节因子1和2基因在人肝癌细胞系中的表达分析

International Hepatology Communications Pub Date : 1996-04-01 DOI: 10.1016/0928-4346(96)00268-X

Kazuhiko Nakao , Keisuke Nakata , Manabu Daikoku , Kaoru Inokuchi , Tasuku Nomura , Akio Ido , Shotaro Tsuruta , Yuji Kato , Nobuko Ishii , Shigenobu Nagataki

引用次数: 0

Cross-reactivity of anti-sulfatide antibody with sulfated glycosaminoglycans and DNA in sera from patients with autoimmune hepatitis 自身免疫性肝炎患者血清中抗硫脂抗体与磺化糖胺聚糖和DNA的交叉反应性

International Hepatology Communications Pub Date : 1996-02-01 DOI: 10.1016/0928-4346(96)00253-8

Y. Ikeda, G. Toda, K. K. Han, N. Hashimoto, H. Yamada, S. Aotsuka

引用次数: 0