International Hepatology Communications最新文献_第3页

Hepatitis G virus/GB virus C infection in patients with chronic non-B, non-C hepatitis 慢性非乙型、非丙型肝炎患者的G型肝炎病毒/GB型肝炎病毒感染

International Hepatology Communications Pub Date : 1997-01-01 DOI: 10.1016/S0928-4346(96)00339-8

Eiji Tanaka , Kenjiro Yamaguchi , Kazuyuki Uemura , Masakazu Kobayashi , Akihiro Iijima , Kendo Kiyosawa , Shintaro Yagi , Akira Hasegawa

{"title":"Hepatitis G virus/GB virus C infection in patients with chronic non-B, non-C hepatitis","authors":"Eiji Tanaka , Kenjiro Yamaguchi , Kazuyuki Uemura , Masakazu Kobayashi , Akihiro Iijima , Kendo Kiyosawa , Shintaro Yagi , Akira Hasegawa","doi":"10.1016/S0928-4346(96)00339-8","DOIUrl":"10.1016/S0928-4346(96)00339-8","url":null,"abstract":"<div><p>A new virus named hepatitis G virus or GB virus C (HGV/GBV-C) was recently identified as a causative agent for acute and chronic hepatitis. In this study, we tested HGV/eGBV-C RNA in serum in addition to hepatitis B virus (HBV) DNA and hepatitis C virus (HCV) RNA to study the causative agents involved in chronic non-B, non-C hepatitis. Of the 1089 patients with histological evidence of chronic viral hepatitis, 25 (2.3%) were negative for both hepatitis B surface antigen and second generation HCV antibody in serum. The 25 patients diagnosed as having chronic non-B, non-C hepatitis, were investigated in this study. HGV/GBV-C a RNA was detected by nested reverse transcription polymerase chain reaction using primers in 5′-untranslated, and the nonstructural (NS) 3 and NS5 regions. Of the 25 patients with chronic non-B, non-C hepatitis, four (16%) were positive for HGV/GBV-C RNA, only one (4%) was positive for HBV DNA, and none were positive for HCV RNA. Of the four patients with HGV/GBV-C RNA, two histologically had mild fibrosis, and the remaining two had cirrhosis. One patient with cirrhosis also had hepatocellular carcinoma, HBV DNA was positive in this patient. All three patients with only the HGV/GBV-C infection had a mild histological grade. In conclusion, HGV/GBV-C infection was involved in 16% of Japanese patients with chronic non-B, non-C hepatitis. Chronic hepatitis G seemed to exhibit mild hepatitis activity.</p></div>","PeriodicalId":13746,"journal":{"name":"International Hepatology Communications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0928-4346(96)00339-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76353877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Immunohistochemical study of tumor necrosis factor-alpha in acute liver injury induced by Propionibacterium acnes and lipopolysaccharide in rats 痤疮丙酸杆菌和脂多糖诱导大鼠急性肝损伤中肿瘤坏死因子- α的免疫组化研究

International Hepatology Communications Pub Date : 1997-01-01 DOI: 10.1016/S0928-4346(96)00347-7

Shin-ichiro Sato , Tomoyuki Masuda , Takashi Satoh , Kazuyuki Suzuki

{"title":"Immunohistochemical study of tumor necrosis factor-alpha in acute liver injury induced by Propionibacterium acnes and lipopolysaccharide in rats","authors":"Shin-ichiro Sato , Tomoyuki Masuda , Takashi Satoh , Kazuyuki Suzuki","doi":"10.1016/S0928-4346(96)00347-7","DOIUrl":"10.1016/S0928-4346(96)00347-7","url":null,"abstract":"<div><p>The effect of intravenous injection of <em>Propionibacterium acnes (P. acnes)</em> and lipopolysaccharide (LPS) on the distribution of tumor necrosis factor-α (TNF-α) in different organs have not previously been investigated. Immunohistochemistry and histological examination were employed in evaluating the distribution of TNF-α in the liver, spleen, lungs and bone marrow in rats injected intravenously with <em>P. acnes</em> followed by LPS 7 days later. Granulomas containing ED1-positive macrophages were observed in the liver 7 days after <em>P. acnes</em> injection. Subsequent LPS injection resulted in proliferation of ED1-positive macrophages in the sinusoids and coagulation necrosis of hepatocytes after 6 h. TNF-α was detected in ED2-positive macrophages (Kupffer cells) 1 day after <em>P. acnes</em> injection and in macrophages constituting the granulomas 7 days later, but prior to LPS injection. TNF-α was also detected in ED1-positive macrophages in the spleen, predominantly in the marginal zone. When granulomas were formed 7 days after <em>P. acnes</em> injection, TNF-α was observed in macrophages of the granulomas. TNF-α was also detected in macrophages of the granulomas found in the lung 1 day after <em>P. acnes</em> injection. No macrophages expressing TNF-α were found in the granulomas of bone marrow. The highest expression was in the liver at any time interval and in macrophages constituting granulomas. Our results suggest that the high expression of TNF-α in the liver results in selective hepatic necrosis. The expression of TNF-α in macrophages of the liver after <em>P. acnes</em> injection and the subsequent development of hepatic necrosis after LPS injection suggest that <em>P. acnes</em> acts as an inducer of TNF-α production in macrophages while LPS acts as a trigger for the release of TNF-α from macrophages.</p></div>","PeriodicalId":13746,"journal":{"name":"International Hepatology Communications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0928-4346(96)00347-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90749465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Effect of organic anions on acyl chain composition of secreted biliary lecithin in rats: relation to hepatocellular vesicle pathway 有机阴离子对大鼠分泌胆磷脂酰基链组成的影响:与肝细胞囊泡通路的关系

International Hepatology Communications Pub Date : 1997-01-01 DOI: 10.1016/S0928-4346(96)00348-9

Hiroyuki Miura, Susumu Tazuma, Hiroaki Miyake, Tsuyoshi Kajihara, Naomichi Hirano, Yoshihiro Hattori, Denya Tsuchimoto, Tomoji Nishioka, Hideyuki Hyogo, Gunji Yamashita, Goro Kajiyama

{"title":"Effect of organic anions on acyl chain composition of secreted biliary lecithin in rats: relation to hepatocellular vesicle pathway","authors":"Hiroyuki Miura, Susumu Tazuma, Hiroaki Miyake, Tsuyoshi Kajihara, Naomichi Hirano, Yoshihiro Hattori, Denya Tsuchimoto, Tomoji Nishioka, Hideyuki Hyogo, Gunji Yamashita, Goro Kajiyama","doi":"10.1016/S0928-4346(96)00348-9","DOIUrl":"10.1016/S0928-4346(96)00348-9","url":null,"abstract":"<div><p>Organic anions can uncouple bile salt secretion from the secretion of phospholipid and cholesterol. The uncoupling mechanism appears to be dependent on the type of anion present. To investigate the effect that different uncoupling mechanisms may have on the flow of bile and its composition, rats were depleted of bile salt pool by overnight biliary diversion and reinfused intravenously with sodium taurocholate (STC) at a constant rate (160 nmol/min per 100 g b.w.). After bile salt secretion had achieved a steady state, sulfobromophthalein (BSP) or papaverine was continuously administered at a rate of 100 nmol/min per 100 g b.w. with STC. During the intravenous infusion of BSP or papaverine, the output of biliary lecithin and cholesterol decreased without affecting bile salt output. The degree of fatty acyl chain saturation in biliary lecithin was increased by BSP infusion and decreased by papaverine infusion. The mechanism by which bile salt secretion is uncoupled from the secretion of phospholipid and cholesterol affects the acyl chain composition of secreted lecithin. The changes in composition of lecithin acyl chains may reflect the intervention by different anions at different points in the lipid transport pathway.</p></div>","PeriodicalId":13746,"journal":{"name":"International Hepatology Communications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0928-4346(96)00348-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87112398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Downregulation of matrix and β-PDGF receptor gene expression by anti-TGFβ antibody in rat hepatic stellate cells during experimental liver injury 抗tgf β抗体对实验性肝损伤大鼠肝星状细胞基质及β-PDGF受体基因表达的下调

International Hepatology Communications Pub Date : 1997-01-01 DOI: 10.1016/S0928-4346(96)00341-6

Victor Ankoma-Sey , Christina Tzagarakis , Kun Bee Chang , Scott L. Friedman

{"title":"Downregulation of matrix and β-PDGF receptor gene expression by anti-TGFβ antibody in rat hepatic stellate cells during experimental liver injury","authors":"Victor Ankoma-Sey , Christina Tzagarakis , Kun Bee Chang , Scott L. Friedman","doi":"10.1016/S0928-4346(96)00341-6","DOIUrl":"10.1016/S0928-4346(96)00341-6","url":null,"abstract":"<div><p>Excess matrix in hepatic fibrosis results from both fibrogenic stimulation of stellate cells by TGFβ1 and cell proliferation due to induction of β-platelet derived growth factor receptor (β-PDGFR). In this paper, treatment of culture-activated rat stellate cells with anti-TGFβ inhibited collagen and fibronectin mRNA expression by 82 and 58%, respectively, versus control cells. In vivo, anti-TGFβ inhibited collagen I gene expression by 86% in stellate cells isolated from rats treated with CC14 compared with control antibody. In contrast to stellate cells, anti-TGFβ had no effect on collagen I gene expression in isolated sinusoidal endothelial cells. Anti-TGFβ administered in vivo to rats with liver injury also reduced expression of stellate cell β-PDGFR mRNA to that of control animals. Anti-TGFβ antibody had no effect on the histologic appearance of the tissue. These data support a role for TGFβ in stellate cell matrix expression and provide evidence for transmodulation of PDGF receptor by TGFβ in vivo. However, inhibition of TGFβ alone may not be adequate to attenuate severe hepatic injury and fibrosis.</p></div>","PeriodicalId":13746,"journal":{"name":"International Hepatology Communications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0928-4346(96)00341-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91098131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Therapeutic effect of preS2-containing vaccine in an infant infected with a vaccine-escape variant (Thr127) of hepatitis B virus 含pres2疫苗对乙型肝炎病毒疫苗逃逸变体(Thr127)感染婴儿的治疗效果

International Hepatology Communications Pub Date : 1997-01-01 DOI: 10.1016/S0928-4346(96)00343-X

H. Noto , Y. Fujii , K. Takahashi , S. Kishimoto , S. Mishiro

{"title":"Therapeutic effect of preS2-containing vaccine in an infant infected with a vaccine-escape variant (Thr127) of hepatitis B virus","authors":"H. Noto , Y. Fujii , K. Takahashi , S. Kishimoto , S. Mishiro","doi":"10.1016/S0928-4346(96)00343-X","DOIUrl":"https://doi.org/10.1016/S0928-4346(96)00343-X","url":null,"abstract":"<div><p>A male baby born to HBV-infected mother received HBIG and vaccine (S-gene recombinant) according to the conventional protocol for prevention of vertical HBV transmission, but did not show active production of anti-HBs. Additional two shots of S-gene recombinant vaccines at months 9 and 12 also failed to induce antibody despite increased doses. Then, at months 14, 15, and 19, a preS2-containing vaccine was injected, followed by a prompt seroconversion to anti-HBs. Retrospective analyses of the baby's sera revealed that HBV DNA was repeatedly positive in this baby's sera throughout the period of time when anti-HBs antibody remained negative (months 6–15). Genetic analyses of HBV DNA from the mother and her baby showed that: (i) mother's HBV DNA was a mixture of <em>adw</em> and <em>adr</em> subtypes, whereas baby's HBV DNA consisted solely of <em>adr</em> subtype; and (ii) baby's HBV was a S-gene variant with its 127th codon coding for threonine instead of proline that is typical to wild type HBV/<em>adr</em> (Thr<sup>127</sup>). Thus, it seems in this case that the Thr<sup>127</sup> mutant did escape conventional vaccines but could not escape preS2-induced immune response, and that eventually the preS2-containing vaccine worked as a therapeutic vaccine.</p></div>","PeriodicalId":13746,"journal":{"name":"International Hepatology Communications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0928-4346(96)00343-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92098645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Conserved nucleotide sequence of NS5A in hepatitis G virus or GB virus C in Japan and China 日本和中国G型肝炎病毒和GB型肝炎病毒NS5A的保守核苷酸序列

International Hepatology Communications Pub Date : 1997-01-01 DOI: 10.1016/S0928-4346(96)00338-6

Toshikazu Hada , Weidong Liu , Hiroyasu Imanishi , Kohya Fukui , Xiaohui Zhou , Zhancheng Yao , Qianhao Wu , Jiewen Qiu , Masao Ohno , Arata Iwasaki , Yoshiki Amuro , Yinrong Wu , Kazuya Higashino

{"title":"Conserved nucleotide sequence of NS5A in hepatitis G virus or GB virus C in Japan and China","authors":"Toshikazu Hada , Weidong Liu , Hiroyasu Imanishi , Kohya Fukui , Xiaohui Zhou , Zhancheng Yao , Qianhao Wu , Jiewen Qiu , Masao Ohno , Arata Iwasaki , Yoshiki Amuro , Yinrong Wu , Kazuya Higashino","doi":"10.1016/S0928-4346(96)00338-6","DOIUrl":"10.1016/S0928-4346(96)00338-6","url":null,"abstract":"<div><p>A semi-nested PCR method was designed for detecting the NS5A region of hepatitis G virus (HGV) or GB virus C (GBV-C) using specific oligonucleotide primers. Patients, 14 of 69, suffering from hepatic diseases in Japan and 5 of 21 in China showed strong positive signals for HGV or GBV-C by this method. The PCR products were directly sequenced. The nucleotide sequences identified differed from one another by 0–5%, from HGV by 3–9% and GBV-C by 2–7%, the percentages of the latter two being compared with the sequences reported previously. Amino acid sequences were the same or had only one alteration compared with HGV and two to three alterations compared with GBV-C. These results indicate that the nucleotide sequence of NS5A of HGV (or GBV-C) is more conserved than that of NS3 of GBV-C (or HGV) which has been reported to show variations of 0–23%.</p></div>","PeriodicalId":13746,"journal":{"name":"International Hepatology Communications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0928-4346(96)00338-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81285078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Detection of telomerase RNA component in human liver tissues 人肝组织端粒酶RNA成分的检测

International Hepatology Communications Pub Date : 1997-01-01 DOI: 10.1016/S0928-4346(96)00344-1

Hisashi Hisatomi, Yoshimi Ohmura, Kumi Nagao, Naomi Mori, Emi Wada, Yasuko Kawaoto, Toshiyasu Yamauchi, Kazumasa Hikiji

{"title":"Detection of telomerase RNA component in human liver tissues","authors":"Hisashi Hisatomi, Yoshimi Ohmura, Kumi Nagao, Naomi Mori, Emi Wada, Yasuko Kawaoto, Toshiyasu Yamauchi, Kazumasa Hikiji","doi":"10.1016/S0928-4346(96)00344-1","DOIUrl":"10.1016/S0928-4346(96)00344-1","url":null,"abstract":"<div><p>This study investigated the relationship between telomerase activity and telomerase RNA component (telomerase RNA) in human liver tissues. Telomerase RNA was detected in all hepatocellular carcinoma (HCC) tissues (<span><math><mtext>20</mtext><mtext>20</mtext></math></span>), and telomerase activity was detected in 18 (90.00%) of the 20 HCC tissues. Telomerase RNA was detected in 17 (89.47%) of 19 livers with liver cirrhosis, and telomerase activity was detected in eight (42.11%) of the 19 tissues. In livers with chronic hepatitis, telomerase RNA was detected in 11 (64.71%) of 17, and telomerase activity was detected in six (35.29%) of the 17 tissues. Telomerase RNA was detected in all the tissues with telomerase activity detected, i.e. 19 HCC tissues, 17 liver cirrhosis tissues and 11 chronic hepatitis tissues. In all normal tissues (<em>n</em> = 3), telomerase RNA and activity were not detected. From our results, one can deduce that the production of telomerase is thought to be under the control of telomerase RNA, the possibility is great that chronic hepatitis tissues and liver cirrhosis tissues become HCC when the telomerase RNA is positive.</p></div>","PeriodicalId":13746,"journal":{"name":"International Hepatology Communications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0928-4346(96)00344-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78649556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Increased cytokine production is associated with acute inflammation in cirrhotic alcoholic patients 肝硬化酒精患者细胞因子产生增加与急性炎症有关

International Hepatology Communications Pub Date : 1997-01-01 DOI: 10.1016/S0928-4346(96)00346-5

Sandra Hirsch, Carlos Muñoz, María Pía de la Maza, Margarita Petermann, Marcelo López, Liana Schlesinger, Daniel Bunout

{"title":"Increased cytokine production is associated with acute inflammation in cirrhotic alcoholic patients","authors":"Sandra Hirsch, Carlos Muñoz, María Pía de la Maza, Margarita Petermann, Marcelo López, Liana Schlesinger, Daniel Bunout","doi":"10.1016/S0928-4346(96)00346-5","DOIUrl":"10.1016/S0928-4346(96)00346-5","url":null,"abstract":"<div><p>The role of cytokines in the etiology of liver injury and their contribution to the systemic manifestations that occur in patients with liver disease, are not clearly understood. <em>Aim:</em> To study if serum levels and in vitro blood mononuclear cell (BMC) production of IL-Iβ and TNFα are related to the severity of alcoholic liver cirrhosis, and identify potential factors that can modify cytokine production in these patients. Serum levels, spontaneous and in vitro LPS stimulated BMC production of Interleukin-Iβ (IL-Iβ) and Tumor Necrosis Factor α (TNFα), were measured in 38 patients with alcoholic cirrhosis Child B or C, and nine normal volunteers. Serum levels and spontaneous in vitro production of IL-Iβ and TNFα were below detection limits. There were no differences between normal controls and Child B or C patients in LPS-stimulated production of IL-Iβ or TNFα. However eight patients with alcoholic hepatitis or infections superimposed on cirrhosis, had higher levels on LPS-stimulated BMC production of IL-Iβ (12.9 ± 5.8 ng/ml) and TNFα (4.9 ± 2.3 ng/ml) than the rest of cirrhotic patients (5.3 ± 3.5 and 1.8 ± 0.9 ng/ml). There was no association between IL-Iβ and TNFα BMC production and parameters of liver function or recent alcohol ingestion. Increased levels of IL-Iβ and TNFα production by stimulated BMC are associated with acute inflammatory events in cirrhotic alcoholic patients and not with the severity of liver disease.</p></div>","PeriodicalId":13746,"journal":{"name":"International Hepatology Communications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0928-4346(96)00346-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81540425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Doxazosin given into the renal arteries of patients with cirrhosis: effects on renal sodium handling, hemodynamics and hormones 肝硬化患者肾动脉给予多沙唑嗪:对肾钠处理、血流动力学和激素的影响

International Hepatology Communications Pub Date : 1997-01-01 DOI: 10.1016/S0928-4346(97)00353-8

Bente Jespersen , Jan Abrahamsen , Poul Christensen , Niels Fogh-Andersen , Paul Schlichting , Helmer Ring-Larsen

{"title":"Doxazosin given into the renal arteries of patients with cirrhosis: effects on renal sodium handling, hemodynamics and hormones","authors":"Bente Jespersen , Jan Abrahamsen , Poul Christensen , Niels Fogh-Andersen , Paul Schlichting , Helmer Ring-Larsen","doi":"10.1016/S0928-4346(97)00353-8","DOIUrl":"10.1016/S0928-4346(97)00353-8","url":null,"abstract":"<div><p>Excessive sodium retention in cirrhosis is believed to be mediated mainly through sympathetic <em>α</em><sub>1</sub>-adrenergic effects of renal nerves supplying renal proximal tubules and renal vessels. Since previous studies on blockage of these nerves have been unequivocal due to confounding systemic effects and contralateral renal counterregulation, we administered doxazosin, an <em>α</em><sub>1</sub>-adrenoceptor antagonist, over 1 h into both renal arteries of four patients with decompensated cirrhosis. During the first 30 min of doxazosin infusion, when blood pressure was only slightly reduced, there was no evidence of improvement in sodium and water excretion. During the subsequent periods, blood pressure, renal plasma flow, glomerular filtration rate, and urinary output declined. The proximal tubular output of sodium, estimated on the lithium clearance, was initially unchanged, but decreased later during the infusion. Hyperreabsorption of sodium seemed to occur more markedly in the distal than in the proximal part of the nephron and was not alleviated by doxazosin. Urinary prostaglandin E<sub>2</sub> excretion was high but decreased in all patients after doxazosin. The study was terminated prematurely because the expected beneficial effects of doxazosin were clearly lacking, as renal function deteriorated temporarily in all the subjects studied. In conclusion, doxazosin given into the renal arteries of patients with decompensated cirrhosis did not improve the renal ability to excrete sodium. The results suggest that additional factors besides renal <em>α</em><sub>1</sub>-sympathetic activity play a major role in the renal sodium retention of patients with cirrhosis.</p></div>","PeriodicalId":13746,"journal":{"name":"International Hepatology Communications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0928-4346(97)00353-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85263798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Development of local necrosis, side-effects and histopathological study after hepatic injection of acetic acid solutions 肝内注射醋酸溶液后局部坏死的发生、副作用及组织病理学研究

International Hepatology Communications Pub Date : 1997-01-01 DOI: 10.1016/S0928-4346(96)00342-8

Toru Tamai , Toshihito Seki , Masato Imamura , Taiichi Nakagawa , Akira Nishimura , Kouji Kunieda , Tomohiro Shiro , Kyoichi Inoue , Akiharu Okamura

{"title":"Development of local necrosis, side-effects and histopathological study after hepatic injection of acetic acid solutions","authors":"Toru Tamai , Toshihito Seki , Masato Imamura , Taiichi Nakagawa , Akira Nishimura , Kouji Kunieda , Tomohiro Shiro , Kyoichi Inoue , Akiharu Okamura","doi":"10.1016/S0928-4346(96)00342-8","DOIUrl":"10.1016/S0928-4346(96)00342-8","url":null,"abstract":"<div><p>Intratumoral injection of 50% acetic acid solution has recently been performed as a new local therapy for hepatocellular carcinoma. The use of ethanol in this therapy has problems of high dosage, frequent injections, and local tumor recurrence. Probably, in these regards the usefulness of acetic acid with potent coagulating and necrotizing effects cannot be denied. We have previously reported the acute toxicity of acetic acid injected into the normal rat liver at various concentrations. In the present study, we assessed the acute toxicity and necrotizing effect of 50% acetic acid solution and its effect on other organs after injection into the normal rat liver in different volumes. With increases in the volume of acetic acid, the size of the necrotic zone tended to increase and the mortality rate rose. The LD<sub>50</sub> of 50% acetic acid calculated with Behrens' method was 0.279 ml/kg. Histopathological examination of rats died immediately after acetic acid injection revealed acute pulmonary edema and marked congestion of the kidneys and spleen. Therefore, if this agent is used clinically, sufficient attention must be paid to its dose level and its acute toxicity.</p></div>","PeriodicalId":13746,"journal":{"name":"International Hepatology Communications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0928-4346(96)00342-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87946171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0