{"title":"Pharmacological Studies on Pentoxifylline and Silymarin in Male Albino rats","authors":"M. Khairy, M. Kamel, H. Mohammed, Aya Zagzoug","doi":"10.21608/zvjz.2023.174032.1192","DOIUrl":"https://doi.org/10.21608/zvjz.2023.174032.1192","url":null,"abstract":"","PeriodicalId":137314,"journal":{"name":"Zagazig Veterinary Journal","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125566891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Oludairo, M. Zakariya, Julius Olaniyi Aiyedun, Oluwafemi Babatunde Daodu, I. Olorunshola, U. Akpabio
{"title":"Prevalence of Salmonella Species in Locally Fermented Milk (Nono) in Gambari Market, Ilorin East Local Government, Kwara State, Nigeria","authors":"O. Oludairo, M. Zakariya, Julius Olaniyi Aiyedun, Oluwafemi Babatunde Daodu, I. Olorunshola, U. Akpabio","doi":"10.21608/zvjz.2023.188459.1205","DOIUrl":"https://doi.org/10.21608/zvjz.2023.188459.1205","url":null,"abstract":"Although milk is highly nutritious, it is also an excellent substrate for microorganisms’ growth like Salmonella which is one of the most common causes of foodborne disease worldwide. This research aimed to isolate Salmonella spp. from nono sold in Gambari market, Ilorin, Kwara State, Nigeria, test the isolated Salmonella spp. to ten antimicrobial agents and evaluate the risk factors associated with the production and retailing processes of nono in the study area. These were achieved by collecting 113 nono samples and analysing them using conventional isolation methods, confirming the suspected isolates using Microbact GNB 24E. Disc diffusion method for antimicrobial sensitivity test and structured questionnaires were also used. Six (5.3%) Salmonella spp. were isolated. All (100%) the Salmonella isolates were resistant to amoxicillin, ampicillin and penicillin. Three (50%) of the isolates had resistance breakpoints of 14mm or less to colistin. Five resistance patterns were recorded among the isolates. Eighty percent of the respondents did not wash their hands before milking, while 70% didn't clean the udder before milking. Self-administration of antibiotics to milking cows by 85% of respondents was also recorded. The isolation of Salmonella , including multiple-antimicrobial-resistant species, in nono in the study area could be an indication of contamination.","PeriodicalId":137314,"journal":{"name":"Zagazig Veterinary Journal","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125032600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Talaat, Mohamed El Beskawy, S. Atwa, Mohamad Eissa, Yassser Mahmmod, M. Elkady, M. El-Diasty
{"title":"Prevalence and Antibiogram of Staphylococcus aureus in Clinical and Subclinical Mastitis in Holstein Dairy Cows in Egypt","authors":"H. Talaat, Mohamed El Beskawy, S. Atwa, Mohamad Eissa, Yassser Mahmmod, M. Elkady, M. El-Diasty","doi":"10.21608/zvjz.2023.162367.1191","DOIUrl":"https://doi.org/10.21608/zvjz.2023.162367.1191","url":null,"abstract":"","PeriodicalId":137314,"journal":{"name":"Zagazig Veterinary Journal","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131959732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Oludairo, J. Kwaga, J. Kabir, P. Abdu, A. Gitanjali, Ann Perrets, V. Cibin, A. Lettini, Julius Olaniyi Aiyedun, Oluwafemi Babatunde Daodu, I. Olorunshola, U. Akpabio
{"title":"Transmission of Salmonella in Humans and Animals and its Epidemiological Factors","authors":"O. Oludairo, J. Kwaga, J. Kabir, P. Abdu, A. Gitanjali, Ann Perrets, V. Cibin, A. Lettini, Julius Olaniyi Aiyedun, Oluwafemi Babatunde Daodu, I. Olorunshola, U. Akpabio","doi":"10.21608/zvjz.2023.187316.1202","DOIUrl":"https://doi.org/10.21608/zvjz.2023.187316.1202","url":null,"abstract":"","PeriodicalId":137314,"journal":{"name":"Zagazig Veterinary Journal","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121866298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of Alogliptin and L-carnitine on Nephrotoxicity-Inducedby Gentamicin in Rats","authors":"Sahar Sakr, M. Kamel","doi":"10.21608/zvjz.2023.176497.1194","DOIUrl":"https://doi.org/10.21608/zvjz.2023.176497.1194","url":null,"abstract":"This research aims to investigate the nephro-protective effects of alogliptin and the supportive effects of L-carnitine in nephrotoxicity produced by gentamicin in male Wistar rats when coupled with a dipeptidyl pepetidase-4 (DDP-4) inhibitor. Five equal groups (G) of 25 male albino rats, each weighing 130 ± 5.7 g, were created: G1 (control negative), G2 (control positive, nephrotoxic), G3 (L-carnitine-treated group), G4 (Alogliptin-treated group),and G5 (treated with alogliptin and L-carnitine). Significant variations were found in the biochemical analyses of the serum total protein, albumin, urea, uric acid, and creatinine. ( P ˂ 0.05) among groups, and the mean value of these parameters revealed that G5 was significantly different from G3 and G4. The concentration of malondialdehyde (MDA), glutathione peroxidase (GPx), catalase (CAT), and super oxide dismutase (SOD) were measured in order to determine the oxidative/antioxidant cascades. The mean value of these parameters revealed significant differences ( P < 0.05) between various groups, with G5 being significantly different from G3 and G4. In gentamicin-induced nephrotoxicity models in rats, the findings of the histological and histochemical analyses suggest that alogliptin and L-carnitine may have a role in preventing the destruction of renal tissue. L-carnitine or alogliptin therapyappeared to preserve the kidney by its antioxidant effect as evidenced by the enhancement of biochemical indices, oxidant state, as well as the recovery of the kidney structural stability and its function.When the two medications are used together, the results are better than using each one separately. The improvement of physiological markers and antioxidant state, as well as the restoration of the kidney's structural integrity and function, demonstrate how administration of alogliptin or L-carnitine maintains the renal through their antioxidant effects. The results are better when the two drugs are taken together rather than individually.","PeriodicalId":137314,"journal":{"name":"Zagazig Veterinary Journal","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132443763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Rapid Reliable EID50 Determination for live Newcastle Disease Viruses and Vaccines","authors":"A. Eid, A. Ismail, H. Gouda, Reham M Elbakrey","doi":"10.21608/zvjz.2022.179646.1196","DOIUrl":"https://doi.org/10.21608/zvjz.2022.179646.1196","url":null,"abstract":"Newcastle disease (ND) was first recognized more than nine decades ago and continues to be a problem for poultry producers besides being enzootic in many countries including Egypt. Diagnostic and ND virus/vaccine titration are core objects in virus evaluation. A rapid assay based on micro plate hemagglutination (HA) activity was applied to investigate its reliability as alternative method for virus titration. A comparative determination of EID50 /0.1ml was carried out via chicken embryo (CE) inoculation for 35 Newcastle disease virus (NDV) strains. They are previously identified by pathogenicity indices and revealed variable virulence (lentogenicmesogenic and velogenic). The data for both methods were analyzed using SPSS version 25. A Wilcoxon signed-rank test showed that HA titer method did not elicit a statistically significant change in median of reading Virus titer of samples with the median of standard embryonated chicken egg (ECE) method (Z = -0.197, p = 0.844). Spearman’s correlation coefficient (r= 0.42, p =0.01) showed a noteworthy moderate correlation between two methods. Heat map showed the differences between each pair of methods and the relationship between them. Bland-Altman plot revealed difference which fit normality distribution W=0.96, p=0.24. Accordingly, the use of HA activity assay for NDV/vaccine titration is a rapid easy and reliable, especially when needed for primary evaluation.","PeriodicalId":137314,"journal":{"name":"Zagazig Veterinary Journal","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114296896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Khalifa Ahmed, MohamedMze Ahmed, Hussein Elbelbesy, A. Arisha
{"title":"Possibility of Dietary Induction of METHFR rs1801133 (SNP) and the Potential Preventive Effect of Curcumin and Metformin in the Presence of Metabolic Syndrome","authors":"Khalifa Ahmed, MohamedMze Ahmed, Hussein Elbelbesy, A. Arisha","doi":"10.21608/zvjz.2022.167049.1190","DOIUrl":"https://doi.org/10.21608/zvjz.2022.167049.1190","url":null,"abstract":"Obesity, especially the visceral one, insulin resistance, atherogenic dyslipidemia, and hypertension are considered metabolic disorder, but the constellation of at least three of these metabolic disorders can be identified collectively as metabolic syndrome (MetS), The prevalence of this syndrome is mostly driven by the current changes in the social environment, behavior, and lifestyle, which is generally called a sedentary lifestyle since more and more individuals are being diagnosed with it.The main target of this article is the collection of some available known published papers aboutthe evaluation of dietary induction of metabolic syndrome accompanied with MTHFR rs1801133(MTHFR–Ala222Val)Singlenucleotide polymorphism (SNP) and the potential preventive effect of metformin or curcumin in male albino rat with an induced MetS as a novel method using many biochemical, Anthropometrical, and molecular biological tests including the embedding the RFLP technique for identification of the presence of the target SNP and its role both in the induction of this gene polymorphism and its role in MetS treatment.","PeriodicalId":137314,"journal":{"name":"Zagazig Veterinary Journal","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121340228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Dietary Alpha-Lipoic Acid Effects on The Mitigation of the Negative Impact of Heat Stress in Broilers","authors":"Hamad Elsaadawi, Arwa Ismaeel, T. Kamal","doi":"10.21608/zvjz.2022.156750.1188","DOIUrl":"https://doi.org/10.21608/zvjz.2022.156750.1188","url":null,"abstract":"The goal of the current study was to assess how α-lipoic acid (ALA) mitigates the negative effects of heat stress (HS) on various biochemical parameters and antioxidant status. For 35 days, a total of 72 one-day-old chicks with an initial body weight average of 45±3 gm were divided into four groups: TNC: no supplements were provided in diet (control group); TN-ALA: 25 gm ALA /100 kg of feed was supplemented; HS-Control: no supplements were added, and the birds were exposed to heat stress; and (HS-ALA: 25 gm ALA/100 kg of diet was supplemented and the birds were exposed to heat stress). From the 21 st day to the end of the experiment, heat stress groups were subjected to (40±5˚C) for eight hours each day (from 7 a.m. to 3 p.m.). Four healthy birds were randomly chosen from each group and slaughtered at the end of the trial for sampling and analysis. Body weight, body weight gain, and feed conversion ratio were not significantly increased by the addition of ALA, while feed intake was significantly raised. When compared with HS-Control group, the dietary addition of ALA considerably lowered the serum total protein and albumin. ALT and AST activities were increased by heat stress unlike with ALA treatment, ALT and AST considerably dropped. The level of serum uric acid and urea decreased while creatinine was not considerably impacted. The addition of ALA to the diet resulted in a considerable decrease in serum total lipids. Malondialdehyde (MDA) dropped noticeably while catalase enzyme activity was elevated. The glucagon like peptide-1 ( GLP-1 ), peroxisome proliferator-activated receptor gamma coactivator 1 alpha ( PGC-1 ), superoxide dismutase-1( SOD-1 ) and mucin-2 ( MUC-2 ) gene expression levels were boosted dramatically in groups supplemented with ALA. The findings indicated that a meal rich in ALA had an impact on some biochemical variables, improved the antioxidant status and boosted the level of genes expression ( GLP-1 , PGC-1α , SOD-1 and MUC-2 ).","PeriodicalId":137314,"journal":{"name":"Zagazig Veterinary Journal","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125158500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Elsadek, Mohamed Elshehidy, M. Shahein, Mohamed Maarof, M. Soltan
{"title":"Molecular Characterization of Bovine Rotaviruses and Coronaviruses in Diarrheic Calves in Egypt (2014-2019)","authors":"E. Elsadek, Mohamed Elshehidy, M. Shahein, Mohamed Maarof, M. Soltan","doi":"10.21608/zvjz.2022.179675.1197","DOIUrl":"https://doi.org/10.21608/zvjz.2022.179675.1197","url":null,"abstract":"Bovine Rotaviruses group A (BRVA) and Bovine Coronaviruses (BCoV) are the most prevalent viral agent worldwide in diarrheic calves aged less than 6 weeks, causing economic losses due to retarded growth, increased susceptibility to other infections, treatment cost, and calf mortalities. This study aimed to detect and molecularly characterize BRVA and BCoV from diarrhetic calves. A total of 82 fecal samples were collected from calves aged less than one month from three Egyptian governorates (Alexandria, Ismailia, and Sharqia). All fecal samples were tested for BRVA and BCoV by using probe based quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Consequently, positive samples contain relatively high viral genomic load were examined by RT-PCR for amplification of viral protein 7 (VP7) and viral protein 4 (VP4) genes (G- and P- typing) for BRV and full length S1 gene for BCoV. Out of 82 of tested samples, 14 (17.1%) and 22 (26.8%) were positive by qRT-PCR for BRV and BCoV, respectively. Only three and five samples had relatively high genomic load for BRV and BCoV, respectively for further testing by RT-PCR. BRV G-type was found in two samples and P-type was detected in one sample. The sequence analysis and phylogenetic tree typed these positive samples as P11 and G10. The sequences and phylogenetic analysis of BCoV positive strains (n=5) showed closely related viruses to each other and similar to previously characterized strains in Egypt since 2014. Further studies are required to antigenically characterize the circulating BRV and BCoV in Egypt.","PeriodicalId":137314,"journal":{"name":"Zagazig Veterinary Journal","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125772575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Z. El-Bouhy, A. A. Abdel Rahman, M. Wahbah, Shaimaa A. A. Ahmed
{"title":"The Immuno-Antioxidant and Anti-bacterial Effects of Clove Powder on Proteus mirabilis Challenge in Oreochromis niloticus:A Comparative Study with Cephalexin Antibiotic","authors":"Z. El-Bouhy, A. A. Abdel Rahman, M. Wahbah, Shaimaa A. A. Ahmed","doi":"10.21608/zvjz.2022.179715.1198","DOIUrl":"https://doi.org/10.21608/zvjz.2022.179715.1198","url":null,"abstract":"A.A","PeriodicalId":137314,"journal":{"name":"Zagazig Veterinary Journal","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133254343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}