International endodontic journal最新文献

{"title":"Virtual reality and haptic simulation in modern microsurgical endodontics: Case report and proof of concept.","authors":"Damiano Pasqualini, Giorgia Carpegna, Mario Alovisi, Elio Berutti, Sami Chogle","doi":"10.1111/iej.14239","DOIUrl":"https://doi.org/10.1111/iej.14239","url":null,"abstract":"<p><strong>Aim: </strong>To highlight the development and application of a novel virtual reality (VR) haptic simulation program in endodontic microsurgery (EMS) to prepare for a clinical case performed by a resident student.</p><p><strong>Summary: </strong>Modern EMS requires adequate training and a learning curve for mastering surgical techniques and refining individual skills. VR haptic simulation is gaining attention in dentistry. Pre-clinical virtual training may enhance skills and minimize the risk of unintentional iatrogenic damage during surgery. VR simulation offers an innovative approach, allowing for the saving, reviewing and repetition of exercises without constraints. In this case report, a female patient presented with persistent apical periodontitis related to an endodontically treated maxillary right second premolar. Utilizing a validated digital workflow, cone beam-computed tomography images were converted into STL file format and optimized for VR simulation. The Virteasy Editor interface was used to generate graphics and touchable haptic solids representing different tissues (enamel, dentine, root canal filling and bone). Following the creation of the virtual patient and the simulation of the endodontic lesion, a postgraduate student in endodontics executed 10 sessions of ostectomy, root-end resection and ultrasonic retro preparation in the evaluation stage until reaching ideal standards of practice in the virtual scenario. The student then proceeded to perform supervised root-end surgery on the actual patient. A 1-year postoperative CBCT evaluated the healing outcomes.</p>","PeriodicalId":13724,"journal":{"name":"International endodontic journal","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143993294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does gender make a difference in endodontic care delivery?","authors":"Victoria Soo Hoon Yu,&nbsp;Thomas Kvist","doi":"10.1111/iej.14240","DOIUrl":"10.1111/iej.14240","url":null,"abstract":"<p>Gender differences are not often emphasized in endodontic literature, and any impact on endodontic understanding and practice is therefore unknown. In this narrative review, we will examine the broader concept of gender and sex-role stereotypes in relation to uncertainty and seek to identify gaps in endodontic shared decision-making. Gender differences in oral health status and patient and clinician choices are highlighted. The importance of effective communication between men and women in shared decision-making as a future research direction is described. We also propose a systematic approach to identify sources of uncertainty in endodontic treatment, formulate probabilities of favourable and unfavourable outcomes based on available best evidence and take steps to minimize gender bias towards equitable and timely care for patients.</p>","PeriodicalId":13724,"journal":{"name":"International endodontic journal","volume":"58 9","pages":"1282-1288"},"PeriodicalIF":7.1,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/iej.14240","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The use of coaching to enhance students' learning, self-efficacy and performance in endodontics.","authors":"Kathryn Fox, Fadi Jarad, Annemarie Baaij","doi":"10.1111/iej.14241","DOIUrl":"https://doi.org/10.1111/iej.14241","url":null,"abstract":"<p><p>The positive effects of coaching on enhancing performance and development within sport have been well recognized over the past 40 years. More recently, the beneficial effects of utilizing a coaching approach upon learning have been recognized in the commercial world and, increasingly, in education. The potential of using a coaching approach within medical education has been investigated, with observed improvements in performance, academic success, resilience, professional identity and self-efficacy - all of which are important in the development of clinicians. Endodontic education and training, whether undergraduate or postgraduate, aim to prepare clinicians to undertake high-quality endodontic care for patients. To achieve this, the student needs to have the capability to perform the appropriate level of endodontic treatments, but also the confidence and self-efficacy to execute those treatments, where self-efficacy contributes to how successful their performances will be. This paper outlines the sources and development of self-efficacy and describes how a coaching approach can be used to enhance students' skills acquisition and performance, both during clinical teaching sessions and in their periodic development reviews.</p>","PeriodicalId":13724,"journal":{"name":"International endodontic journal","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144016266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Representation of women in executive and academic roles within scientific societies in the field of European Endodontology","authors":"Ana Arias,&nbsp;Henry F. Duncan,&nbsp;Ruth Perez Alfayate,&nbsp;Jenifer Martín-González,&nbsp;Paula Riaza","doi":"10.1111/iej.14237","DOIUrl":"10.1111/iej.14237","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To explore gender distribution within the field of Endodontology in Europe and to evaluate the representation of women in executive and academic roles within scientific societies as well as national and international conferences.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methodology</h3>\u0000 \u0000 <p>After ethical approval, an online questionnaire was developed and shared with the European Society of Endodontology and the 36 national Endodontic societies officially affiliated. The survey inquired about gender composition amongst members, executive committee roles, participation in major congresses and representation in leadership and authorship positions within scientific journals affiliated with these societies over the last 5 years. Information was also retrieved from publicly available websites. Genders of the first, last and corresponding authors from scientific journals were identified. The relative ratio of women amongst society members, executive committee roles, participation in major congresses and representation in leadership and authorship positions within scientific journals was calculated. The ratio of women in leadership positions was statistically compared with the mean relative ratio of women amongst society members using a one-sample t-test. The current proportion of women in individual roles was compared with the general proportion of women members using the Binomial test.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Women account for 44% of members in endodontic societies. No significant discrepancy was observed in the overall representation of women as members in executive or editorial committees or in authorship positions within scientific journals in relation to the mean relative ratio of female society members but are significantly underrepresented in key positions such as society presidents, treasurers and scientific event organizers (<i>p</i> &lt; .05). Additionally, women were significantly underrepresented at scientific events, both as lecturers and workshop leaders, at major congresses of national/international societies (<i>p</i> &lt; .05). Few initiatives have been implemented to address these inequities; only three societies reported programmes aimed at promoting women in leadership or scientific roles.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Within the limitations of this study, women appear to be fairly represented as members of executive and editorial committees in national endodontic societies and the ESE, as well as in authorship positions within scientific journals, but underrepresentation ","PeriodicalId":13724,"journal":{"name":"International endodontic journal","volume":"58 9","pages":"1301-1312"},"PeriodicalIF":7.1,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/iej.14237","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144015050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Irrigant Procedures for Treating Apical Periodontitis (TIPTAP): A triple-blinded parallel-group randomized controlled phase I/II trial","authors":"Satnam Singh Virdee,&nbsp;Nasir Zeeshan Bashir,&nbsp;Melissa M. Grant,&nbsp;Paul R. Cooper,&nbsp;Phillip L. Tomson","doi":"10.1111/iej.14233","DOIUrl":"10.1111/iej.14233","url":null,"abstract":"&lt;div&gt;\u0000 \u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Background&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Solubilized endogenous dentine extracellular matrix components (dECMs) are potent mediators in pulp regeneration and could potentially promote similar healing effects in diseased periradicular tissues by upregulating local mesenchymal stem cell-derived regenerative events.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Aims&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;(1) Determine if endodontic treatment outcomes with irrigation regimes promoting dECM release (17% ethylenediaminetetraacetic acid [EDTA]) are equivalent to conventional regimes (5.25% sodium hypochlorite [NaOCl]) in mature permanent teeth with asymptomatic apical periodontitis. (2) Explore changes in pain scores, expressions of periradicular tissue fluid (PTF)-derived inflammatory mediators, and volumtric changes in lesion size between the different irrigant regimes.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Methods&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Forty single-rooted teeth, from 37 healthy adults, were block randomized into parallel groups of irrigation with either 17% EDTA, optimized for dECM solubilization, or 5.25% NaOCl (&lt;i&gt;n&lt;/i&gt; = 20). All other aspects of the endodontic protocol were standardized over two visits with 14 days of calcium hydroxide intracanal medicament. Patient-reported pain scores were recorded at six hours and then daily for one week post-instrumentation and post-obturation. PTF samples were collected pre-instrumentation and pre-obturation, where analyte profiles (pg/TPC) were determined using an O-link Target-48 cytokine array. Treatment outcomes were clinically and radiographically assessed with cone beam computed tomography at 1 year using dichotomous criteria (favourable/unfavourable) based on volumetric change in lesion size. Participants, operators and assessors were blinded, and per-protocol analyses were conducted using binary logistic regression models with initial alpha values for statistical comparisons set at &lt;i&gt;p&lt;/i&gt; &lt; .05.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Results&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;A 90% recall rate was achieved at one year (NaOCl: 19; EDTA: 17). Favourable outcomes were observed in 89.5% of treatments using NaOCl and 94.1% of treatments using EDTA irrigation, with median lesion volume reductions of 92.5% (IQR: 67.33–99.13) and 95.84% (IQR: 78.81–100), respectively, (&lt;i&gt;p&lt;/i&gt; &gt; .05). Odds of unfavourable periradicular healing with EDTA irrigation were 0.53 [95% CI: 0.04–6.44; &lt;i&gt;p&lt;/i&gt; &gt; .05]. No serious adverse effects or atypical pain patterns were reported, although two acute exacerbations occurred post-instrumentation with NaOCl irrigation (&lt;i&gt;p&lt;/i&gt; &gt; .05). Target-48 panels consistently detected","PeriodicalId":13724,"journal":{"name":"International endodontic journal","volume":"58 7","pages":"965-990"},"PeriodicalIF":5.4,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/iej.14233","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144006607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparison of traditional and virtual reality haptic simulator approaches in preclinical endodontic training: Impacts on skill acquisition, confidence and stress.","authors":"Sıla Nur Usta, Emmanuel João Nogueira Leal Silva, Cangül Keskin, Hilal Tekkanat, Mikko Liukkonen, Szabolcs Felszeghy","doi":"10.1111/iej.14236","DOIUrl":"https://doi.org/10.1111/iej.14236","url":null,"abstract":"<p><strong>Aim: </strong>This study aimed to evaluate the influence of virtual reality (VR) haptic simulators on manual dexterity, self-confidence and stress levels of 3rd-semester dental students during preclinical endodontic training, as well as to determine the optimal timing for their introduction into the curriculum-before or after training with artificial teeth.</p><p><strong>Methodology: </strong>Forty volunteered dental students enrolled in preclinical endodontic training were randomly divided into two groups. Group 1 (VR Haptic Simulator group, n = 20) completed four sessions with VR haptic simulators, including three training sessions followed by an evaluation session, before practicing on artificial teeth, whilst Group 2 (Control group, n = 20) performed a single attempt on artificial teeth first followed by the identical VR haptic simulator training period as Group 1. Prior to the training sessions, both groups received standardized lectures and demonstrations on access cavity preparation. Pre- and post-training evaluations included stress levels measured via a visual analog scale (VAS) at different phases, assessment of manual dexterity through preclinical training with artificial teeth performance scores, and self-confidence surveys. Statistical analyses were conducted with significance set at p < .05.</p><p><strong>Results: </strong>Group 1 demonstrated significantly higher manual dexterity scores and self-assessed proficiency compared to Group 2 (p < .05). Stress levels, measured via VAS, were notably reduced following simulator practice in both groups, with Group 1 reporting lower anxiety levels during preclinical training with artificial teeth sessions (p < .05). Simulator-based performance metrics were comparable between the groups (p > .05). Self-confidence surveys indicated that Group 1 felt better prepared and more confident in performing access cavity preparations and managing procedural challenges.</p><p><strong>Conclusion: </strong>The integration of VR haptic simulators before preclinical training with artificial teeth has a positive effect on students' manual dexterity along with reduced stress and increased self-confidence in clinical skills. Further research is needed to explore long-term effects and optimal integration strategies for VR simulators in dental curricula.</p>","PeriodicalId":13724,"journal":{"name":"International endodontic journal","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144013494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A re-usable 3D-printed training model for the endodontic management of dental trauma in immature teeth.","authors":"Eva Maier, Jan Zentgraf, Eveline Angele, José Zorzin, Michael Taschner, Matthias Widbiller, Kerstin Galler, Thomas Schratzenstaller","doi":"10.1111/iej.14235","DOIUrl":"https://doi.org/10.1111/iej.14235","url":null,"abstract":"<p><strong>Aim: </strong>To develop a 3D-printed model enabling treatment simulation of trauma-related endodontic and restorative procedures like revitalization or placement of an apical plug on teeth with incomplete root formation.</p><p><strong>Methodology: </strong>To generate a realistic training model suitable for trauma-related simulations, CT-Data sets were segmented, combined, adjusted and optimized using computer-aided design features. Specific focus was on optimally reflecting characteristics of immature teeth like open apical foramina and thin root walls. For reflection of the revitalization process, a set-up including a tube filled with red liquid under pressure was developed to be perforated during the procedure by the trainee, simulating bleeding from the apical papilla into the root canal system. Design was based on a combination of cost-effective simple parts combined with 3D-printed components to achieve maximal accessibility, exchangeability and re-usability. Model assembly and preparation were described in step-by-step instructions.</p><p><strong>Results: </strong>As a result of the developmental process, the presented model qualifies for the training of the endodontic management of complications on immature teeth after dental trauma. Step-by-step descriptions aligned with the clinical procedures are performable for a revitalization procedure, for pulpotomy followed by fragment re-attachment and splint application and for placement of an apical plug using hydraulic calcium silicate cements.</p><p><strong>Conclusions: </strong>The model successfully achieved its intended objectives, so that it may be used in the future in various dental trauma treatment simulations for undergraduate and postgraduate education on the endodontic management of trauma-related therapies in immature teeth. Its common manufacturing process, affordability and re-usability support accessibility and sustainability.</p>","PeriodicalId":13724,"journal":{"name":"International endodontic journal","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143997844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Letter to Editor for “Potential predisposing features of external cervical resorption: An observational study (Patel et al., 2025)”","authors":"John Rong Hao Tay,&nbsp;Upul Cooray","doi":"10.1111/iej.14215","DOIUrl":"https://doi.org/10.1111/iej.14215","url":null,"abstract":"","PeriodicalId":13724,"journal":{"name":"International endodontic journal","volume":"58 5","pages":"797-798"},"PeriodicalIF":5.4,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143801693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of ovariectomy-induced osteoporosis and two photobiomodulation protocols on the repair of apical periodontitis in Wistar rats: A microCT study","authors":"Carolina Horn Troian-Michel,&nbsp;Bruna Barcelos Só,&nbsp;Lauren Frenzel Schuch,&nbsp;Grazielle Oliveira Stelter,&nbsp;Tuany Rafaeli Schmidt,&nbsp;Joana Letícia Schorr,&nbsp;Daniela Campagnol,&nbsp;Tuane Nerissa Alves Garcez,&nbsp;Gabriel Barcelos Só,&nbsp;Thalita Ayres Arrué,&nbsp;Theodoro Weissheimer,&nbsp;Manoela Domingues Martins,&nbsp;Marcus Vinicius Reis Só","doi":"10.1111/iej.14234","DOIUrl":"10.1111/iej.14234","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>Osteoporosis is a disease that is related to a higher prevalence and greater volumes of apical periodontitis. The purpose of this study was to evaluate the effect of ovariectomy-induced osteoporosis and high- or low-level laser photobiomodulation (PBM) on the repair of apical periodontitis (AP) in adult female Wistar rats.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methodology</h3>\u0000 \u0000 <p>Sixty female Wistar rats (<i>n</i> = 120) were randomly allocated into four control (<i>n</i> = 6 teeth) and 8 experimental (<i>n</i> = 12 teeth) groups, according to procedure and time of evaluation: healthy control (GCS14/GCS28), osteoporotic control (GCO14/GCO28), sham (SHAM14/SHAM28), osteoporotic (OVX14/OVX28), low-level laser (OVX-LLL14/OVX-LLL28) and high-level laser (OVX-GEM14/OVX-GEM28). All groups were submitted to bilateral ovariectomy, except for the healthy control and sham. Eight weeks later, AP was induced bilaterally in mandibular first molars' mesial roots in experimental groups, and after 21 days, their endodontic treatment (RCT) was conducted. For 14 or 28 days, PBM was applied three times/week, as follows: sham application (SHAM and OVX groups); low-level laser (OVX/LLL groups); defocused high-power laser (OVX/GEM groups). After the respective experimental periods, the animals were euthanized. Tibias and hemi-mandibula were obtained and submitted to computed microtomography. Bone volume fraction (BV/TV), trabeculae number (Tb.N), spacing (Tb.Sp) and thickness (Tb.Th) were obtained for tibias, while RCT apical limit, periodontal ligament and AP volumes (APvol) were recorded for hemi-mandibula. Data were subjected to statistical analysis through generalized estimating equations (GEE), ANOVA and chi-square tests (5% significance level).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Ovariectomy tibias showed lower BV/TV and Tb.N (<i>p</i> &lt; .0001) and higher Tb.Th (<i>p</i> = .020) and Tb.Sp (<i>p</i> &lt; .0001) when compared to sham groups. For hemi-mandibula, lower APvol values were found in SHAM14 when compared to OVX/LLL14 and OVX/GEM14 groups (<i>p</i> &lt; .05), which were not different from each other. Over the 28-day period, the OVX/LLL28 group showed higher AP volumes when compared to OVX28 and OVX/GEM28 (<i>p</i> &lt; .05). Analysing variables effects on APvol, time, PBM and RCT apical limit showed no effect (<i>p</i> &gt; .05), while ovariectomy showed a significant increase (<i>p</i> = .004).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Ovariectomy-induced osteoporosis in Wistar rats led to a reduction in AP rep","PeriodicalId":13724,"journal":{"name":"International endodontic journal","volume":"58 7","pages":"1042-1059"},"PeriodicalIF":5.4,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/iej.14234","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electric field promoted odontogenic differentiation of stem cells from apical papilla by remodelling cytoskeleton","authors":"Xiaolin Li,&nbsp;Sanjun Zhao,&nbsp;Yao Liu,&nbsp;Yu Gu,&nbsp;Lihong Qiu,&nbsp;Xu Chen,&nbsp;Alastair J. Sloan,&nbsp;Bing Song","doi":"10.1111/iej.14213","DOIUrl":"10.1111/iej.14213","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>This study examined the impact of direct current electric fields (DCEFs) on the biological properties of stem cells derived from the apical papilla (SCAP) and further elucidated the underlying mechanisms involved in odontogenic differentiation induced by DCEFs stimulation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methodology</h3>\u0000 \u0000 <p>The measurement of endogenous currents in wounded dentine was achieved using a non-invasive vibrating probe system. Two-dimensional (2D) and three-dimensional (3D) systems were developed to apply DCEFs of varying strengths. The migration direction and trajectories of SCAP within DCEFs were analysed using time-lapse imaging. Cell proliferation was assessed through Hoechst staining and the CCK-8 assay. Changes in cell morphology, arrangement, and polarization were examined using fluorescence staining. The odontogenic differentiation of SCAP in vitro was assessed using quantitative polymerase chain reaction (qPCR), western blot analysis, alkaline phosphatase staining, and Alizarin Red S staining. In vivo evaluation was conducted through Haematoxylin and eosin staining, immunohistochemistry staining, and Sirius Red staining after transplantation experiments.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Injured dentine demonstrated a significantly increased outward current, and DCEFs facilitated the migration of SCAP towards the anode. DCEFs at a magnitude of 100 mV/mm promoted SCAP proliferation, whereas DCEFs at 200 mV/mm enhanced both polarization and odontogenic differentiation of SCAP. The application of cytoskeletal polymerization inhibitors mitigated the odontogenic differentiation induced by DCEFs. In vivo studies confirmed that DCEFs promoted the differentiation of SCAP into odontoblast-like cells in an orderly arrangement, as well as the formation of collagen fibres and dentine-like tissue.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>DCEFs of varying intensities exhibited an enhanced capacity for migration, proliferation, odontogenic differentiation, and polarization in SCAP. These findings provide substantial insights for the advancement of innovative therapeutic strategies targeting the repair and regeneration of immature permanent teeth and dentine damage.</p>\u0000 </section>\u0000 </div>","PeriodicalId":13724,"journal":{"name":"International endodontic journal","volume":"58 6","pages":"873-889"},"PeriodicalIF":5.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/iej.14213","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143763086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}