IF 5 3区医学

Immunology Pub Date : 2025-09-09 DOI: 10.1111/imm.70034

Hsueh-Yen Lu, Ping-Hsiang Huang, Ting-Wei Lee, Hui-Wen Chang, Nai-Yu Chen, Yu-Jing Zhuang, Ta-Tung Yuan, Chun-Jen Chen

{"title":"Enolase-1 Is a Key Regulator of Neutrophil Recruitment During Acute Inflammation.","authors":"Hsueh-Yen Lu, Ping-Hsiang Huang, Ting-Wei Lee, Hui-Wen Chang, Nai-Yu Chen, Yu-Jing Zhuang, Ta-Tung Yuan, Chun-Jen Chen","doi":"10.1111/imm.70034","DOIUrl":"https://doi.org/10.1111/imm.70034","url":null,"abstract":"Enolase-1 (ENO1) is a moonlighting protein with multiple functions. When expressed on the cell surface, ENO1 binds plasminogen (PLG) and promotes cell migration by facilitating plasmin (PLM)-mediated extracellular matrix degradation. Here, we observed that inflammatory stimulation significantly upregulated ENO1 expression on the neutrophil surface, both in vitro and in vivo. An anti-ENO1 monoclonal antibody (mAb), 7E5, which blocks the ENO1-PLG interaction, effectively suppressed neutrophil invasion in vitro. In mouse models of acute inflammation, including lipopolysaccharide (LPS)-induced lung injury and necrotic cell challenge, 7E5 treatment markedly reduced neutrophil recruitment and neutrophil extracellular trap (NET) formation. Similarly, the PLG inhibitor tranexamic acid (TXA) attenuated neutrophil recruitment, confirming the critical role of the PLG/PLM system in neutrophil migration. These findings highlight ENO1 as a key regulator of inflammation and neutrophil infiltration. Targeting ENO1 with antibodies could be a promising strategy to mitigate tissue damage caused by excessive neutrophilic inflammation.","PeriodicalId":13508,"journal":{"name":"Immunology","volume":" ","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145023163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dendritic Cell-Derived Extracellular Vesicles as Therapeutic Cancer Vaccines: Mechanisms and Optimization Strategies. 树突状细胞衍生的细胞外囊泡作为治疗性癌症疫苗：机制和优化策略。

IF 5 3区医学

Immunology Pub Date : 2025-09-02 DOI: 10.1111/imm.70033

Jonathan Shpigelman, Krishna Rao

引用次数: 0

Microneedle-Delivered Multivalent MPXV DNA Vaccines Induce Promising Immunity Profiles and Cross-Protection in Mice. 微针递送的多价MPXV DNA疫苗在小鼠中诱导有希望的免疫谱和交叉保护

IF 5 3区医学

Immunology Pub Date : 2025-09-02 DOI: 10.1111/imm.70030

Yawei Wang, Xueting Cheng, Baoying Huang, Ruixiao Tan, Feng Fan, Li Zhao, Wenling Wang, Fei Ye, Yao Deng, Xiaoming Gao, Bin Wang, Wenjie Tan

{"title":"Microneedle-Delivered Multivalent MPXV DNA Vaccines Induce Promising Immunity Profiles and Cross-Protection in Mice.","authors":"Yawei Wang, Xueting Cheng, Baoying Huang, Ruixiao Tan, Feng Fan, Li Zhao, Wenling Wang, Fei Ye, Yao Deng, Xiaoming Gao, Bin Wang, Wenjie Tan","doi":"10.1111/imm.70030","DOIUrl":"https://doi.org/10.1111/imm.70030","url":null,"abstract":"Traditional DNA vaccines, typically administered via intramuscular injection with electroporation (IM-E), often cause discomfort and require trained personnel. Addressing these challenges, we developed multivalent DNA vaccines targeting both intracellular mature virion (IMV) and extracellular enveloped virion (EEV) proteins of the monkeypox virus (MPXV), designated as M2 (A29L, B6R), M3 (A29L, B6R, M1R) and M4 (A29L, B6R, M1R, A35R). These vaccine constructs were formulated into dissolvable microneedle array patches (D-MAPs) for intradermal delivery. Comparative studies in mice demonstrated that D-MAPs achieved approximately 70% delivery efficiency and elicited robust humoral immune responses in mice, including antigen-specific IgG and cross-neutralising antibodies against MPXV, VACV and ECTV-comparable to those induced by IM-E. Furthermore, D-MAP immunisation induced stronger T cell responses, particularly in the draining lymph nodes. Importantly, the multivalent DNA vaccines-especially M3 and M4-conferred substantial protection against lethal VACV-WR challenge, achieving levels of protection comparable to the traditional replication-competent smallpox vaccine TianTan (VTT), with significant viral suppression and mitigation of pathological damage. Collectively, this study provided valuable insights for the development of innovative MPXV DNA vaccines, highlighting a minimally invasive and suitable for field application with D-MAP with broad potential for combating mpox outbreaks and future orthopoxvirus pandemics.","PeriodicalId":13508,"journal":{"name":"Immunology","volume":" ","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144952584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tertiary Lymphoid Structures Predict Prognosis and Immune Checkpoint Inhibitor Efficacy in Lung Squamous Cell Carcinoma. 三级淋巴结构预测肺鳞癌的预后和免疫检查点抑制剂的疗效。

IF 5 3区医学

Immunology Pub Date : 2025-08-15 DOI: 10.1111/imm.70027

Kuifei Chen, Suna Zhou, Pin Zhou, Zhenwei Sun, Yixiu Xu, Ziran Chen, Liangmin Zhang, Wenhu Pi, Meifu Gan, Haihua Yang

{"title":"Tertiary Lymphoid Structures Predict Prognosis and Immune Checkpoint Inhibitor Efficacy in Lung Squamous Cell Carcinoma.","authors":"Kuifei Chen, Suna Zhou, Pin Zhou, Zhenwei Sun, Yixiu Xu, Ziran Chen, Liangmin Zhang, Wenhu Pi, Meifu Gan, Haihua Yang","doi":"10.1111/imm.70027","DOIUrl":"https://doi.org/10.1111/imm.70027","url":null,"abstract":"Lung squamous cell carcinoma (LUSC) is a highly mortal cancer. Tertiary lymphoid structures (TLSs) play a crucial role in creating a specific and essential environment for the development of cellular and humoral immune responses against tumours. This study investigated the effect of TLSs on prognosis and the prediction of immunotherapy efficacy in LUSC. To investigate the association between TLSs and clinicopathological features, haematoxylin and eosin staining and multiple immunofluorescence staining were performed. A comparative examination of survival and the factors influencing it was carried out between the TLS-/+, high and low TLS density, immature tertiary lymphoid structures (imTLS) and mature tertiary lymphoid structures (mTLS) groups of patients. To further analyse the prognostic value of TLSs in the immunotherapy cohort of patients with LUSC, two hundred and ninety-seven patients with LUSC were enrolled in this study. Of these, 129 patients were harbouring TLS+. Cramer's V relationships analysis revealed that both Stage (p = 0.029) and PD-L1 ≥ 50% (p = 0.011) were significant predictors of TLS+. Cox proportional hazards model multivariate analysis showed that the TLS+ (p = 0.037), high TLS density (p = 0.014) and mTLS (p = 0.001) were associated with better overall survival (OS) in LUSC patients. Multivariate analyses confirmed that TLS+ was an independent prognostic predictor for OS in the LUSC immunotherapy cohort (p = 0.021). This study provided evidence that LUSC patients with TLS+, high TLS density and mTLS had a favourable prognosis, suggesting that TLSs are an independent positive prognostic factor for LUSC patients.","PeriodicalId":13508,"journal":{"name":"Immunology","volume":" ","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144859081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-Existence of IL6ST and MEFV Pathogenic Variant in a Patient With Hyper-IgE Syndrome. 高ige综合征患者IL6ST和MEFV致病变异共存

IF 5 3区医学

Immunology Pub Date : 2025-08-12 DOI: 10.1111/imm.70029

Hulya Kose, Muruvvet Yanaz, Akcahan Akalin

引用次数: 0

Exercise Duration Modulates Cortisol Release and Chronic Cortisol Exposure Jeopardises T Cell Effector Functions. 运动时间调节皮质醇释放，慢性皮质醇暴露损害T细胞效应功能。

IF 5 3区医学

Immunology Pub Date : 2025-08-12 DOI: 10.1111/imm.70028

Thy Viet Luu, Line Fleischer Hach, Tina Seremet, Katharina Leuchte, Per Thor Straten, Gitte Holmen Olofsson

{"title":"Exercise Duration Modulates Cortisol Release and Chronic Cortisol Exposure Jeopardises T Cell Effector Functions.","authors":"Thy Viet Luu, Line Fleischer Hach, Tina Seremet, Katharina Leuchte, Per Thor Straten, Gitte Holmen Olofsson","doi":"10.1111/imm.70028","DOIUrl":"https://doi.org/10.1111/imm.70028","url":null,"abstract":"Psychological stress has been linked to increased incidence and mortality of cancer. During stress, cortisol is released into circulation and regulates cellular processes including immune activity by acting on glucocorticoid receptors (GCRs) expressed by target cells. Chronic stress-induced cortisol has been suggested to promote tumour progression and compromise the efficacy of cancer treatments. Conversely, cortisol is also transiently secreted during exercise. Although exercise has been suggested to have beneficial effects against cancer, the impact of exercise-elevated cortisol on immune cell functions remains poorly understood. Here we studied the dynamics of cortisol secretion following exercise and how cortisol affects effector functions of T cells in the context of acute versus chronic stress. We show that 40 min of acute, high-intensity exercise in healthy adults significantly increased stable circulating cortisol levels whereas a 5-min sprint failed to. Acute exposure to cortisol for 4 h showed no negative effects on the proliferation, cytokine release, or killing activity of human CD3+ T cells. In contrast, chronic cortisol dampened these T cell effector functions. Furthermore, chronic cortisol exposure induced the proliferation of several cancer cell lines. Our findings highlight the opposing effects of cortisol during acute stress, such as exercise, compared to chronic stress, on cancer cells and T cells. This suggests an important potential in targeting cortisol signalling to enhance cancer immunotherapy.","PeriodicalId":13508,"journal":{"name":"Immunology","volume":" ","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144835006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

27-Hydroxycholesterol Exacerbates the Pathogenesis of Asthma. 羟基胆固醇加剧哮喘的发病机制。

IF 5 3区医学

Immunology Pub Date : 2025-08-11 DOI: 10.1111/imm.70026

Ya Li, Xingyue Liu, Feifei Shang, Minzhu Niu, Jiaqi Yan, Minyu Xie, Xiangnan Tao, Han Huang, Wenwen Wu, Shu Dong, Yingzi Chen, Fan Wu, Shujun Guo, Yulin Du, Mengqing Hua, Chuanwang Song

{"title":"27-Hydroxycholesterol Exacerbates the Pathogenesis of Asthma.","authors":"Ya Li, Xingyue Liu, Feifei Shang, Minzhu Niu, Jiaqi Yan, Minyu Xie, Xiangnan Tao, Han Huang, Wenwen Wu, Shu Dong, Yingzi Chen, Fan Wu, Shujun Guo, Yulin Du, Mengqing Hua, Chuanwang Song","doi":"10.1111/imm.70026","DOIUrl":"https://doi.org/10.1111/imm.70026","url":null,"abstract":"27-Hydroxycholesterol (27-HC) is an oxidative metabolite of cholesterol and an oxysterol catalysed by the mitochondrial cytochrome P450 enzyme, sterol 27-hydroxylase (CYP27A1). In addition to inducing the release of eosinophil chemotactic factors such as RANTES and Eotaxin, 27-HC enhances the differentiation of lung fibroblasts into myofibroblasts and promotes the production of extracellular matrix proteins. Therefore, it is possible that 27-HC may play a significant role in the pathogenesis of asthma. In this study, we observed elevated expression of CYP27A1 and increased production of 27-HC in the lung tissues of asthmatic mice, with alveolar macrophages (AMs) identified as the primary source of 27-HC. 27-HC induced an increase in total cell count and eosinophil number in the bronchoalveolar lavage fluid of asthmatic mice, exacerbated inflammatory cell infiltration into lung tissues, and heightened airway hyper-responsiveness, thereby aggravating asthma. The alarmin, IL-33, within airways induced 27-HC production by AMs via the NF-κB signalling pathway. Furthermore, 27-HC was shown to inhibit the phagocytosis of apoptotic cells (efferocytosis) by airway epithelial cells (AECs) through AMPK activation. Thus, in asthmatic mice, 27-HC, predominantly derived from AMs, influences the efferocytotic function of AECs, demonstrating that cross-talk between macrophages and epithelial cells regulates asthma pathogenesis. This study provides valuable insight into the molecular mechanisms underlying asthma and offers theoretical and experimental data for identifying novel therapeutic targets for clinical asthma management.","PeriodicalId":13508,"journal":{"name":"Immunology","volume":" ","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144816547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tissue Resident Memory Cells: Friend or Foe? 组织常驻记忆细胞：是敌是友？

IF 5 3区医学

Immunology Pub Date : 2025-08-03 DOI: 10.1111/imm.70024

Chidimma F Chude, Jude E Uzonna, Janilyn Arsenio

{"title":"Tissue Resident Memory Cells: Friend or Foe?","authors":"Chidimma F Chude, Jude E Uzonna, Janilyn Arsenio","doi":"10.1111/imm.70024","DOIUrl":"https://doi.org/10.1111/imm.70024","url":null,"abstract":"Tissue-resident memory T (TRM) cells are a specialised subset of immune cells that remain within tissues, playing a vital role in localised immune defence and long-term immunity. Unlike circulating memory T cells, TRM cells do not recirculate to provide rapid and effective responses against previously encountered pathogens at the tissue level. The formation of TRM cells is driven by tissue-specific cues, guiding their differentiation and retention within organs such as the skin, lungs and gut. They are characterised by the expression of unique markers, including CD69 and CD103, which facilitate their retention and longevity in tissues. TRM cells are essential for immune surveillance, effectively detecting and responding to different infections and contributing to tumour suppression. However, TRM cells are also implicated in chronic inflammatory and autoimmune diseases, where persistent activation by resident and autoantigens can lead to tissue damage. This pathogenic role is evident in chronic inflammatory conditions such as psoriasis, vitiligo and inflammatory bowel disease (IBD), where TRM cells may drive persistent localised inflammation and contribute to disease progression and severity. Emerging therapeutic strategies seek to modulate TRM cells to balance their protective and pathogenic roles in these inflammatory diseases. Approaches such as checkpoint inhibitors, cytokine modulation and cell-depletion therapies aim to enhance TRM cells' beneficial immune functions while minimising their role in autoimmunity. A deeper understanding of TRM cell development, maintenance and functional diversity is critical for advancing treatments for infectious diseases, chronic inflammation, autoimmune conditions and cancer.","PeriodicalId":13508,"journal":{"name":"Immunology","volume":" ","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144775325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral Treatment With Heat Shock Protein 65-Producing Lactococcus lactis Induces Regulatory T Cells, Modulating Inflammatory Response in Leishmania braziliensis Infection. 口服产热休克蛋白65乳球菌诱导调节性T细胞，调节巴西利什曼原虫感染的炎症反应

IF 5 3区医学

Immunology Pub Date : 2025-07-31 DOI: 10.1111/imm.70022

Camila Mattos Andrade, Ítalo da Silva Gonçalves, Maria Luiza das Neves Nascimento, Washington Luís Conrado Santos, Vasco Ariston Azevedo, Deborah Bittencourt Mothé, Juliana Perrone Menezes Fullam, Patrícia Sampaio Tavares Veras, Natalia Machado Tavares, Tatiani Uceli Maioli, Ana Maria Caetano Faria, Cláudia Ida Brodskyn

{"title":"Oral Treatment With Heat Shock Protein 65-Producing Lactococcus lactis Induces Regulatory T Cells, Modulating Inflammatory Response in Leishmania braziliensis Infection.","authors":"Camila Mattos Andrade, Ítalo da Silva Gonçalves, Maria Luiza das Neves Nascimento, Washington Luís Conrado Santos, Vasco Ariston Azevedo, Deborah Bittencourt Mothé, Juliana Perrone Menezes Fullam, Patrícia Sampaio Tavares Veras, Natalia Machado Tavares, Tatiani Uceli Maioli, Ana Maria Caetano Faria, Cláudia Ida Brodskyn","doi":"10.1111/imm.70022","DOIUrl":"https://doi.org/10.1111/imm.70022","url":null,"abstract":"Cutaneous leishmaniasis (CL), a neglected tropical disease prevalent in Brazil, is caused by Leishmania braziliensis (L. braziliensis) and is marked by ulcerative skin lesions and an exacerbated Th1-driven inflammatory response. This study investigates the therapeutic potential of oral tolerance (OT) induced by a genetically modified strain of Lactococcus lactis (L. lactis) producing heat shock protein 65 (HSP65) from Mycobacterium leprae in a murine model of CL. BALB/c mice were infected with L. braziliensis and treated orally with HSP65-producing L. lactis or control L. lactis (empty vector) for four consecutive days, starting at 4 weeks post-infection. Mice receiving HSP65-producing L. lactis showed reduced lesion size and parasite burden. Cytokine analysis in draining lymph nodes revealed a shift from a pro-inflammatory IFN-γ response to an increased IL-10 production, correlating with milder inflammation and less tissue damage. Additionally, the treatment promoted an increase in regulatory T cells (Tregs), including CD4+CD25+FOXP3+ and CD4+LAP+ (membrane-associated TGF-β) cells in the draining lymph nodes. This therapeutic effect was not observed in a more severe model of CL using Leishmania major. This study underscores the potential of oral tolerance induction using HSP65-producing L. lactis as a promising immunoregulatory therapeutic approach for some chronic inflammatory infections, mainly those that display a primed balance in immune response.","PeriodicalId":13508,"journal":{"name":"Immunology","volume":" ","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144760008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to "Pla2g5 Contributes to Viral-Like-Induced Lung Inflammation Through Macrophage Proliferation and LA/Ffar1 Lung Cell Recruitment". 更正“Pla2g5通过巨噬细胞增殖和LA/Ffar1肺细胞募集参与病毒样诱导的肺部炎症”。

IF 4.9 3区医学

Immunology Pub Date : 2025-07-24 DOI: 10.1111/imm.70017

引用次数: 0

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