Biotechnology Journal最新文献_第7页

Issue Information: Biotechnology Journal 8/2024 发行信息：生物技术杂志 8/2024

IF 3.2 3区生物学

Biotechnology Journal Pub Date : 2024-08-08 DOI: 10.1002/biot.202470081

引用次数: 0

Through virtual saturation mutagenesis and rational design for superior substrate conversion in engineered d-amino acid oxidase 通过虚拟饱和诱变和合理设计，在工程化 d- 氨基酸氧化酶中实现卓越的底物转换。

IF 3.2 3区生物学

Biotechnology Journal Pub Date : 2024-07-16 DOI: 10.1002/biot.202400287

Heng Tang, Hong-Li Zhu, Jin-Qiao Zhao, Liu-Yu Wang, Ya-Ping Xue, Yu-Guo Zheng

{"title":"Through virtual saturation mutagenesis and rational design for superior substrate conversion in engineered d-amino acid oxidase","authors":"Heng Tang, Hong-Li Zhu, Jin-Qiao Zhao, Liu-Yu Wang, Ya-Ping Xue, Yu-Guo Zheng","doi":"10.1002/biot.202400287","DOIUrl":"10.1002/biot.202400287","url":null,"abstract":"The d-amino acid oxidase (DAAO) is pivotal in obtaining optically pure l-glufosinate (l-PPT) by converting d-glufosinate (d-PPT) to its deamination product. We screened and designed a Rasamsonia emersonii DAAO (ReDAAO), making it more suitable for oxidizing d-PPT. Using Caver 3.0, we delineated three substrate binding pockets and, via alanine scanning, identified nearby key residues. Pinpointing key residues influencing activity, we applied virtual saturation mutagenesis (VSM), and experimentally validated mutants which reduced substrate binding energy. Analysis of positive mutants revealed elongated side-chain prevalence in substrate binding pocket periphery. Although computer-aided approaches can rapidly identify advantageous mutants and guide further design, the mutations obtained in the first round may not be suitable for combination with other advantageous mutations. Therefore, each round of combination requires reasonable iteration. Employing VSM-assisted screening multiple times and after four rounds of combining mutations, we ultimately obtained a mutant, N53V/F57Q/V94R/V242R, resulting in a mutant with a 5097% increase in enzyme activity compared to the wild type. It provides valuable insights into the structural determinants of enzyme activity and introduces a novel rational design procedure.","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"19 7","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141625467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a marker recyclable CRISPR/Cas9 system for scarless and multigene editing in Fusarium fujikuroi 开发一种标记可回收的 CRISPR/Cas9 系统，用于在 Fusarium fujikuroi 中进行无痕和多基因编辑。

IF 3.2 3区生物学

Biotechnology Journal Pub Date : 2024-07-16 DOI: 10.1002/biot.202400164

Lianggang Huang, Ningning Li, Yixin Song, Jie Gao, Lu Nian, Junping Zhou, Bo Zhang, Zhiqiang Liu, Yuguo Zheng

{"title":"Development of a marker recyclable CRISPR/Cas9 system for scarless and multigene editing in Fusarium fujikuroi","authors":"Lianggang Huang, Ningning Li, Yixin Song, Jie Gao, Lu Nian, Junping Zhou, Bo Zhang, Zhiqiang Liu, Yuguo Zheng","doi":"10.1002/biot.202400164","DOIUrl":"10.1002/biot.202400164","url":null,"abstract":"Iterative metabolic engineering of Fusarium fujikuroi has traditionally been hampered by its low homologous recombination efficiency and scarcity of genetic markers. Thus, the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas9) system has emerged as a promising tool for precise genome editing in this organism. Some integrated CRISPR/Cas9 strategies have been used to engineer F. fujikuroi to improve GA3 production capabilities, but low editing efficiency and possible genomic instability became the major obstacle. Herein, we developed a marker recyclable CRISPR/Cas9 system for scarless and multigene editing in F. fujikuroi. This system, based on an autonomously replicating sequence, demonstrated the capability of a single plasmid harboring all editing components to achieve 100%, 75%, and 37.5% editing efficiency for single, double, and triple gene targets, respectively. Remarkably, even with a reduction in homologous arms to 50 bp, we achieved a 12.5% gene editing efficiency. By employing this system, we successfully achieved multicopy integration of the truncated 3-hydroxy-3-methyl glutaryl coenzyme A reductase gene (tHMGR), leading to enhanced GA3 production. A key advantage of our plasmid-based gene editing approach was the ability to recycle selective markers through a simplified protoplast preparation and recovery process, which eliminated the need for additional genetic markers. These findings demonstrated that the single-plasmid CRISPR/Cas9 system enables rapid and precise multiple gene deletions/integrations, laying a solid foundation for future metabolic engineering efforts aimed at industrial GA3 production.","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"19 7","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141625462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exosomal lncRNA USP30-AS1 activates the Wnt/β-catenin signaling pathway to promote cervical cancer progression via stabilization of β-catenin by USP30 外泌体 lncRNA USP30-AS1 通过 USP30 稳定β-catenin，激活 Wnt/β-catenin 信号通路，促进宫颈癌的进展。

IF 3.2 3区生物学

Biotechnology Journal Pub Date : 2024-07-16 DOI: 10.1002/biot.202300653

Chi Chi, Xiuwu Tang, Wei Liu, Ying Zhou, Rong Jiang, Youguo Chen, Min Li

{"title":"Exosomal lncRNA USP30-AS1 activates the Wnt/β-catenin signaling pathway to promote cervical cancer progression via stabilization of β-catenin by USP30","authors":"Chi Chi, Xiuwu Tang, Wei Liu, Ying Zhou, Rong Jiang, Youguo Chen, Min Li","doi":"10.1002/biot.202300653","DOIUrl":"10.1002/biot.202300653","url":null,"abstract":"Cervical cancer (CC) remains a major cause of cancer-related mortality among women globally. Long noncoding RNAs (lncRNAs) play crucial regulatory roles in various cancers, including CC. This study investigates the function of a novel lncRNA, USP30 antisense RNA 1 (USP30-AS1), in CC tumorigenesis. We analyzed USP30-AS1 expression using RT-qPCR and conducted in vitro loss-of-function assays, as well as in vivo assays, to evaluate the effects of USP30-AS1 silencing on CC cell growth and migration. Additional mechanistic experiments, including RNA pull-down, RNA immunoprecipitation (RIP), and co-immunoprecipitation (Co-IP) assays, were performed to elucidate the regulatory mechanisms influenced by USP30-AS1. We discovered that USP30-AS1 is overexpressed in CC tissues and cells. Silencing USP30-AS1 significantly reduced cell proliferation, migration, invasion, and tumor growth. Moreover, USP30-AS1 was found to modulate the expression of ubiquitin-specific peptidase 30 (USP30) by sponging microRNA-2467-3p (miR-2467-3p) and recruiting the FUS RNA binding protein (FUS), thereby stabilizing β-catenin and activating the Wnt/β-catenin signaling pathway. These findings suggest that USP30-AS1 enhances CC cell growth and migration through the miR-2467-3p/FUS/USP30 axis, highlighting its potential as a biomarker for CC.","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"19 7","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141625465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient production of 22(R)-hydroxycholesterol via combination optimization of Saccharomyces cerevisiae 通过组合优化酿酒酵母高效生产 22(R)-羟基胆固醇。

IF 3.2 3区生物学

Biotechnology Journal Pub Date : 2024-07-16 DOI: 10.1002/biot.202400286

Yaru Pang, Xu Cheng, Yali Ban, Yue Li, Bo Lv, Chun Li

{"title":"Efficient production of 22(R)-hydroxycholesterol via combination optimization of Saccharomyces cerevisiae","authors":"Yaru Pang, Xu Cheng, Yali Ban, Yue Li, Bo Lv, Chun Li","doi":"10.1002/biot.202400286","DOIUrl":"10.1002/biot.202400286","url":null,"abstract":"22(R)-hydroxycholesterol (22(R)-HCHO) is a crucial precursor of steroids biosynthesis with various biological functions. However, the production of 22(R)-HCHO is expensive and unsustainable due to chemical synthesis and extraction from plants or animals. This study aimed to construct a microbial cell factory to efficiently produce 22(R)-HCHO through systems metabolic engineering. First, we tested 7-dehydrocholesterol reductase (Dhcr7s) and cholesterol C22-hydroxylases from different sources in Saccharomyces cerevisiae, and the titer of 22(R)-HCHO reached 128.30 mg L−1 in the engineered strain expressing Dhcr7 from Columba livia (ClDhcr7) and cholesterol 22-hydroxylase from Veratrum californicum (VcCyp90b27). Subsequently, the 22(R)-HCHO titer was significantly increased to 427.78 mg L−1 by optimizing the critical genes involved in 22(R)-HCHO biosynthesis. Furthermore, hybrid diploids were constructed to balance cell growth and 22(R)-HCHO production and to improve stress tolerance. Finally, the engineered strain produced 2.03 g L−1 of 22(R)-HCHO in a 5-L fermenter, representing the highest 22(R)-HCHO titer reported to date in engineered microbial cell factories. The results of this study provide a foundation for further applications of 22(R)-HCHO in various industrially valuable steroids.","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"19 7","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141625464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthetic biology for Monascus: From strain breeding to industrial production 莫纳科合成生物学：从菌种培育到工业化生产

IF 3.2 3区生物学

Biotechnology Journal Pub Date : 2024-07-16 DOI: 10.1002/biot.202400180

Junping Zhou, Qilu Pan, Yinan Xue, Yaping Dong, Yihong Chen, Lianggang Huang, Bo Zhang, Zhi-Qiang Liu, Yuguo Zheng

{"title":"Synthetic biology for Monascus: From strain breeding to industrial production","authors":"Junping Zhou, Qilu Pan, Yinan Xue, Yaping Dong, Yihong Chen, Lianggang Huang, Bo Zhang, Zhi-Qiang Liu, Yuguo Zheng","doi":"10.1002/biot.202400180","DOIUrl":"10.1002/biot.202400180","url":null,"abstract":"Traditional Chinese food therapies often motivate the development of modern medicines, and learning from them will bring bright prospects. Monascus, a conventional Chinese fungus with centuries of use in the food industry, produces various metabolites, including natural pigments, lipid-lowering substances, and other bioactive ingredients. Recent Monascus studies focused on the metabolite biosynthesis mechanisms, strain modifications, and fermentation process optimizations, significantly advancing Monascus development on a lab scale. However, the advanced manufacture for Monascus is lacking, restricting its scale production. Here, the synthetic biology techniques and their challenges for engineering filamentous fungi were summarized, especially for Monascus. With further in-depth discussions of automatic solid-state fermentation manufacturing and prospects for combining synthetic biology and process intensification, the industrial scale production of Monascus will succeed with the help of Monascus improvement and intelligent fermentation control, promoting Monascus applications in food, cosmetic, agriculture, medicine, and environmental protection industries.","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"19 7","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141625466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

UV-based dynamic control improves the robustness of multicolumn countercurrent solvent gradient purification of oligonucleotides 基于紫外的动态控制提高了多柱逆流溶剂梯度纯化寡核苷酸的稳健性。

IF 3.2 3区生物学

Biotechnology Journal Pub Date : 2024-07-16 DOI: 10.1002/biot.202400170

Ismaele Fioretti, Thomas Müller-Späth, Lars Aumann, Mattia Sponchioni

{"title":"UV-based dynamic control improves the robustness of multicolumn countercurrent solvent gradient purification of oligonucleotides","authors":"Ismaele Fioretti, Thomas Müller-Späth, Lars Aumann, Mattia Sponchioni","doi":"10.1002/biot.202400170","DOIUrl":"10.1002/biot.202400170","url":null,"abstract":"Therapeutic oligonucleotides (ONs) have great potential to treat many diseases due to their ability to regulate gene expression. However, the inefficiency of standard purification techniques to separate the target sequence from molecularly similar variants is hindering development of large scale ON manufacturing at a reasonable cost. Multicolumn Countercurrent Solvent Gradient Purification (MCSGP) is a valuable process able to bypass the purity-yield tradeoff typical of single-column operations, and hence to make the ON production more sustainable from both an economic and environmental point of view. However, operating close to the optimum of MCSGP can be challenging, resulting in unstable process performance and in a drift in product quality, especially when running a continuous process for extended periods where process parameters such as temperature are prone to variation. In this work, we demonstrate how greater process robustness is introduced in the design and execution of MCSGP for the purification of a 20mer single-stranded DNA sequence through the implementation of UV-based dynamic control. With this novel approach, the cyclic steady state was reached already in the third cycle and disturbances coming from fluctuations in the feed quality, loading amount and temperature were effectively compensated allowing a stable operation close to the optimum. In response to the perturbations, the controlled process kept the standard deviation on product recovery below 3.4%, while for the non-controlled process it increased up to 27.5%.","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"19 7","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/biot.202400170","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141625468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient generation of recombinant anti-HER2 scFv with high yield and purity using a simple method 用一种简单的方法高效生成高产率、高纯度的重组抗 HER2 scFv。

IF 3.2 3区生物学

Biotechnology Journal Pub Date : 2024-07-16 DOI: 10.1002/biot.202300745

Hanool Yun, Sun-Hee Kim, Seung-Hee Kim, Hae-Min Park, Hee-Jin Jeong

{"title":"Efficient generation of recombinant anti-HER2 scFv with high yield and purity using a simple method","authors":"Hanool Yun, Sun-Hee Kim, Seung-Hee Kim, Hae-Min Park, Hee-Jin Jeong","doi":"10.1002/biot.202300745","DOIUrl":"10.1002/biot.202300745","url":null,"abstract":"We developed a method to produce a soluble form of a single-chain fragment variable (scFv) targeting human epithelial growth factor receptor 2 (HER2) in Escherichia coli. By optimizing the orientations of the variable heavy (VH) and variable light (VL) domains and the His-tag, we identified the HL-His type antibody with the highest HER2-binding activity. Purification of HL-His yielded 40.7 mg from a 1 L culture, achieving >99% purity. The limit of detection was determined to be 2.9 ng, demonstrating high production yield, purity, and sensitivity. Moreover, we successfully labeled HER2+ cell lines with fluorescent dye-conjugated scFv, resulting in a significantly higher observed signal-to-background ratio, compared to that of HER2− cell lines. This highlights the potential of these fluorescent scFvs as valuable probes for HER2+ breast cancer diagnostics. Notably, the process for the complete scFv production was streamlined and required only 4–5 days. Additionally, the product maintained its activity after freeze storage, allowing for large-scale production and a wide range of practical applications.","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"19 7","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/biot.202300745","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141625463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel calibration design improves knowledge transfer across products for the characterization of pharmaceutical bioprocesses 新颖的校准设计改善了制药生物工艺表征的跨产品知识转移。

IF 3.2 3区生物学

Biotechnology Journal Pub Date : 2024-07-12 DOI: 10.1002/biot.202400080

Laura M. Helleckes, Claus Wirnsperger, Jakub Polak, Gonzalo Guillén-Gosálbez, Alessandro Butté, Moritz von Stosch

{"title":"Novel calibration design improves knowledge transfer across products for the characterization of pharmaceutical bioprocesses","authors":"Laura M. Helleckes, Claus Wirnsperger, Jakub Polak, Gonzalo Guillén-Gosálbez, Alessandro Butté, Moritz von Stosch","doi":"10.1002/biot.202400080","DOIUrl":"10.1002/biot.202400080","url":null,"abstract":"Modern machine learning has the potential to fundamentally change the way bioprocesses are developed. In particular, horizontal knowledge transfer methods, which seek to exploit data from historical processes to facilitate process development for a new product, provide an opportunity to rethink current workflows. In this work, we first assess the potential of two knowledge transfer approaches, meta learning and one-hot encoding, in combination with Gaussian process (GP) models. We compare their performance with GPs trained only on data of the new process, that is, local models. Using simulated mammalian cell culture data, we observe that both knowledge transfer approaches exhibit test set errors that are approximately halved compared to those of the local models when two, four, or eight experiments of the new product are used for training. Subsequently, we address the question whether experiments for a new product could be designed more effectively by exploiting existing knowledge. In particular, we suggest to specifically design a few runs for the novel product to calibrate knowledge transfer models, a task that we coin calibration design. We propose a customized objective function to identify a set of calibration design runs, which exploits differences in the process evolution of historical products. In two simulated case studies, we observed that training with calibration designs yields similar test set errors compared to common design of experiments approaches. However, the former requires approximately four times fewer experiments. Overall, the results suggest that process development could be significantly streamlined when systematically carrying knowledge from one product to the next.","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"19 7","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141597931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering a 3-dimensional tissue construct with adipose-derived stem cells for healing bone defect: An ex vivo study with femur head 利用脂肪干细胞构建三维组织，用于骨缺损愈合：股骨头体外研究。

IF 3.2 3区生物学

Biotechnology Journal Pub Date : 2024-07-10 DOI: 10.1002/biot.202300751

Aditi Mahajan, Siddhartha Sharma, Sanjay Kumar Bhadada, Aditya Aggarwal, Shalmoli Bhattacharyya

{"title":"Engineering a 3-dimensional tissue construct with adipose-derived stem cells for healing bone defect: An ex vivo study with femur head","authors":"Aditi Mahajan, Siddhartha Sharma, Sanjay Kumar Bhadada, Aditya Aggarwal, Shalmoli Bhattacharyya","doi":"10.1002/biot.202300751","DOIUrl":"10.1002/biot.202300751","url":null,"abstract":"<div>\u0000 \u0000 The compatibility of bone graft substitutes (BGS) with mesenchymal stem cells (MSCs) is an important parameter to consider for their use in repairing bone defects as it eventually affects the clinical outcome. In the present study, a few commercially available BGS – β-tricalcium phosphate (β-TCP), calcium sulfate, gelatin sponge, and different forms of hydroxyapatite (HAP) were screened for their interactions with MSCs from adipose tissue (ADSCs). It was demonstrated that HAP block favorably supported ADSC viability, morphology, migration, and differentiation compared to other scaffolds. The results strongly suggest the importance of preclinical evaluation of bone scaffolds for their cellular compatibility. Furthermore, the bone regenerative potential of HAP block with ADSCs was evaluated in an ex vivo bone defect model developed using patient derived trabecular bone explants. The explants were cultured for 45 days in vitro and bone formation was assessed by expression of osteogenic genes, ALP secretion, and high resolution computed tomography. Our findings confirmed active bone repair process in ex vivo settings. Addition of ADSCs significantly accelerated the repair process and improved bone microarchitecture. This ex vivo bone defect model can emerge as a viable alternative to animal experimentation and also as a potent tool to evaluate patient specific bone therapeutics under controlled conditions.\u0000 </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"19 7","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141578405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0