促进次生代谢物生物合成的真菌启动子工程研究进展

IF 3.1 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Biotechnology Journal Pub Date : 2025-07-14 DOI:10.1002/biot.70075

Nan Pu, Huawei Zhang

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引用次数: 0

摘要

在过去的二十年中，启动子工程被广泛用于微调基因转录调控，以唤醒或增加真菌基因组中次生代谢物（SM）生物合成基因簇（BGCs）的表达。本文综述了各种真菌启动子工程技术的最新进展，包括启动子替换、优化和组合方法，以及它们在提高SM产量方面的应用。此外，本文还讨论了真菌中用于精确代谢调控的高效启动子工程的当前挑战和未来前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Advances in Fungal Promoter Engineering for Enhancing Secondary Metabolite Biosynthesis

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Advances in Fungal Promoter Engineering for Enhancing Secondary Metabolite Biosynthesis

In the past two decades, promoter engineering has been extensively employed as a powerful strategy for fine-tuning the regulation of gene transcription to awaken or increase the expression of secondary metabolite (SM) biosynthetic gene clusters (BGCs) in fungal genomes. This comprehensive review provides an overview of recent advances in various fungal promoter engineering techniques, including promoter replacement, optimization, and combinatorial methods, and their application in the enhancement of SM production. Additionally, current challenges and future prospects for efficient promoter engineering for precise metabolic regulation in fungi are discussed.

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来源期刊

Biotechnology Journal Biochemistry, Genetics and Molecular Biology-Molecular Medicine

CiteScore

8.90

自引率

2.10%

发文量

123

审稿时长

1.5 months

期刊介绍： Biotechnology Journal (2019 Journal Citation Reports: 3.543) is fully comprehensive in its scope and publishes strictly peer-reviewed papers covering novel aspects and methods in all areas of biotechnology. Some issues are devoted to a special topic, providing the latest information on the most crucial areas of research and technological advances. In addition to these special issues, the journal welcomes unsolicited submissions for primary research articles, such as Research Articles, Rapid Communications and Biotech Methods. BTJ also welcomes proposals of Review Articles - please send in a brief outline of the article and the senior author''s CV to the editorial office. BTJ promotes a special emphasis on: Systems Biotechnology Synthetic Biology and Metabolic Engineering Nanobiotechnology and Biomaterials Tissue engineering, Regenerative Medicine and Stem cells Gene Editing, Gene therapy and Immunotherapy Omics technologies Industrial Biotechnology, Biopharmaceuticals and Biocatalysis Bioprocess engineering and Downstream processing Plant Biotechnology Biosafety, Biotech Ethics, Science Communication Methods and Advances.