Indian Journal of Pathology and Microbiology最新文献_第10页

Molecular characterization and potential therapeutic roles of miR125a in HER-2 positive gastric cancer. miR125a在HER-2阳性癌症中的分子特征和潜在治疗作用。

IF 1 4区医学

Indian Journal of Pathology and Microbiology Pub Date : 2023-07-01 DOI: 10.4103/ijpm.ijpm_580_21

Afraa Mamoori, Zena Hasan Sahib, Haider Alkafaji

{"title":"Molecular characterization and potential therapeutic roles of miR125a in HER-2 positive gastric cancer.","authors":"Afraa Mamoori, Zena Hasan Sahib, Haider Alkafaji","doi":"10.4103/ijpm.ijpm_580_21","DOIUrl":"10.4103/ijpm.ijpm_580_21","url":null,"abstract":"Introduction: miR-125a-3p could have a role in gastric cancer by targeting HER2. This study aimed to investigate the expression pattern of miR-125a-3p, identify the expression level of its target gene in gastric carcinoma, and test its effect in HER-2 positive gastric carcinoma cells.Materials and methods: The levels of miR-125a-3p in both cancer and noncancer tissues were measured by using Quantitative real-time polymerase chain in 70 gastric carcinomas. Immunohistochemical study was used to measure the expression of HER2 protein in these carcinomas. In addition, the level of expression of this miRNA is correlated to different pathological and clinical parameters. The effects of miR-125a-3p alone and in combination with 5-FU (fluorouracil) on the growth of HER2 positive (NUGC4) and HER2 negative (ECC10) gastric carcinoma cells were also analyzed by in vitro studies.Results: Most gastric cancer tissues samples showed downregulation of miR-125a-3p (84%) when compared to their noncancer tissues. Significant correlations of downregulation of miR-125a-3p with cancer recurrence and pathological staging of gastric carcinoma (P = 0. 02 and 0.02, respectively) were noted. HER2 protein expression correlated significantly and inversely with miR-125a-3p expression (P < 0.05). A reduction in cell growth rate was noted significantly in miR-125a-3p transfected gastric carcinoma cells when 5-FU was added to them in comparison to other control cells (P < 0.01). When both gastric carcinoma cell lines were transfected with miR-125a-3p, a significantly higher growth inhibition percentage in HER2 positive (NUGC4) cell line was seen in comparison to the HER2 negative (ECC10) cells (P < 0.01).Conclusion: miR-125a-3p plays a significant role in the pathogenesis of gastric carcinoma. Therapeutic transfection of miR-125a-3p in HER2 positive gastric cancer cells resulted in reduced cell proliferation and potentiate the effect of 5-FU.","PeriodicalId":13488,"journal":{"name":"Indian Journal of Pathology and Microbiology","volume":"66 3","pages":"472-477"},"PeriodicalIF":1.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10278190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The immunohistochemical Galectin-3 expression in tumor and cancer-associated fibroblasts in invasive ductal carcinomas of breast and their relationship with clinicopathological parameters. 乳腺浸润性导管癌肿瘤及癌相关成纤维细胞中半乳糖凝集素-3的免疫组织化学表达及其与临床病理参数的关系。

IF 1 4区医学

Indian Journal of Pathology and Microbiology Pub Date : 2023-07-01 DOI: 10.4103/ijpm.ijpm_284_21

Yasemin Cakir, Canan Kelten Talu, Didem Can Trabulus, Ozlem Mermut

{"title":"The immunohistochemical Galectin-3 expression in tumor and cancer-associated fibroblasts in invasive ductal carcinomas of breast and their relationship with clinicopathological parameters.","authors":"Yasemin Cakir, Canan Kelten Talu, Didem Can Trabulus, Ozlem Mermut","doi":"10.4103/ijpm.ijpm_284_21","DOIUrl":"10.4103/ijpm.ijpm_284_21","url":null,"abstract":"Background: Galectin-3 has an important role in metastasis, therefore, Galectin-3-focused therapies have attracted attention for various cancers.Aim: We aimed to reveal the relationship between the expression of Galectin-3 within the tumor/cancer-associated fibroblasts (CAF) and clinicopathological parameters in patients with invasive ductal carcinomas.Materials and methods: Hematoxylin and eosin-stained slides of breast excision materials diagnosed between 2010 and 2016 were re-examined retrospectively. Accordingly, 118 cases (luminal group = 58, Human epidermal growth factor receptor 2 (HER2) group = 27, and triple-negative breast carcinoma group [TNBC] =33 cases) were included. Galectin-3 levels were evaluated with a calculated H-score in tumor and semiquantitatively in CAFs.Statistical analysis: Data was analyzed with t-tests and Chi-square tests. Kaplan-Meier and Log-rank tests were used for survival analysis.Results: The presence of Galectin-3 expression in CAFs but not in the tumor was associated with the greater number of axillary metastatic nodes and advanced pN stage. The loss of Galectin-3 expression in CAFs was more frequent in TNBC. There was no significant relationship between the expression level of Galectin-3 and survival status. However, in most of the cases with distant metastasis or patients who died, Galectin-3 was negative in the tumor, whereas it was positive in CAFs.Conclusions: The expression of Galectin-3 in tumors and CAFs may have a role in metastasis to axillary lymph nodes and distant sites. In terms of molecular subtype, TNBCs show a relationship with Galectin-3 negativity in CAFs.","PeriodicalId":13488,"journal":{"name":"Indian Journal of Pathology and Microbiology","volume":"66 3","pages":"456-464"},"PeriodicalIF":1.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10296216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oncocytic sialolipoma of parotid gland: Case report and literature review. 腮腺癌细胞性唾液泡：病例报告和文献复习。

IF 1 4区医学

Indian Journal of Pathology and Microbiology Pub Date : 2023-07-01 DOI: 10.4103/ijpm.ijpm_323_21

Venu Patel Sureja, Koyye Ravindranath Tagore

引用次数: 0

Secondary amyloidosis with concomitant gastric GIST and gastric adenocarcinoma - A case report. 继发性淀粉样变性伴胃GIST和胃腺癌一例报告。

IF 1 4区医学

Indian Journal of Pathology and Microbiology Pub Date : 2023-07-01 DOI: 10.4103/ijpm.ijpm_461_21

Ankita Soni, Nishant Batta, Shefali Mehmi, Vikram Narang, Bhavna Garg, Harpreet Kaur

{"title":"Secondary amyloidosis with concomitant gastric GIST and gastric adenocarcinoma - A case report.","authors":"Ankita Soni, Nishant Batta, Shefali Mehmi, Vikram Narang, Bhavna Garg, Harpreet Kaur","doi":"10.4103/ijpm.ijpm_461_21","DOIUrl":"10.4103/ijpm.ijpm_461_21","url":null,"abstract":"Secondary amyloidosis is a well-established entity and has been described in association with chronic inflammatory conditions such as rheumatoid arthritis, ankylosing spondylitis, bronchiectasis, tuberculosis, etc., It has also been reported in association with neoplasms such as Hodgkin's lymphoma, Waldenstrom's macroglobulinemia, renal cell carcinoma, lung carcinoma, etc. However, only a few case reports documenting the association of amyloidosis with gastrointestinal tumor (GIST) and gastric adenocarcinoma are available in the literature. Hereby, we report a case of a 74-year-old male who presented with colicky abdominal pain and vomiting. Ultrasonography revealed a common bile duct (CBD) stone and a small extra-luminal gastric mass. Endoscopic retrograde cholangiopancreatography (ERCP) was performed to remove the CBD stone which revealed an incidental finding of gastric ulcer. A biopsy was taken from the gastric ulcer which on histopathological examination was confirmed as adenocarcinoma leading onto total gastrectomy. During total gastrectomy, an inadvertent injury to the spleen led to simultaneous splenectomy. Multiple samples from the gastric ulcer, the extra-luminal gastric mass, and the spleen were subjected to histopathological examination. Gastric ulcer was confirmed as adenocarcinoma, gastric extra-luminal mass was confirmed as GIST, and splenic examination revealed widespread deposition of amyloid which on Congo-red stain imparted an apple-green birefringence on polarizing microscopy. To the best of our knowledge, this is the first-ever case of such an association where gastric adenocarcinoma occurred with concomitant gastric GIST and secondary amyloidosis of the spleen.","PeriodicalId":13488,"journal":{"name":"Indian Journal of Pathology and Microbiology","volume":"66 3","pages":"594-596"},"PeriodicalIF":1.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9921067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolated tubercular dacryoadenitis in a non-immunocompromised healthy adult. 一名未免疫功能受损的健康成年人的孤立性结核性泪腺炎。

IF 1 4区医学

Indian Journal of Pathology and Microbiology Pub Date : 2023-07-01 DOI: 10.4103/ijpm.ijpm_489_21

Ajax Jossy, N Kaviyapriya, Nirupama Kasturi, J Sree Rekha

引用次数: 0

Pancreatic tuberculosis mimicking as pancreatic malignancy: Surgeon's dilemma. 模拟胰腺恶性肿瘤的胰腺结核：外科医生的两难选择。

IF 1 4区医学

Indian Journal of Pathology and Microbiology Pub Date : 2023-07-01 DOI: 10.4103/ijpm.ijpm_874_21

Rohik Anjum T Siddeek, Amit Gupta, Tanuj Singla, Deepak Rajput, Shaik Sameer Ahmed, Reshma Jeladharan

引用次数: 0

Infantile (congenital) anaplastic intracranial solitary fibrous tumor/hemangiopericytoma-A case report with brief literature review. 婴儿（先天性）间变性颅内孤立性纤维瘤/血管外皮细胞瘤一例报告并简要文献复习。

IF 1 4区医学

Indian Journal of Pathology and Microbiology Pub Date : 2023-07-01 DOI: 10.4103/ijpm.ijpm_643_21

Mohapatra Debahuti, Lenka Anasuya, Deo R Chandra, Das Prateek

{"title":"Infantile (congenital) anaplastic intracranial solitary fibrous tumor/hemangiopericytoma-A case report with brief literature review.","authors":"Mohapatra Debahuti, Lenka Anasuya, Deo R Chandra, Das Prateek","doi":"10.4103/ijpm.ijpm_643_21","DOIUrl":"10.4103/ijpm.ijpm_643_21","url":null,"abstract":"Solitary fibrous tumor/hemangiopericytoma (SFT/HPC) is a rare primary central nervous system (CNS) tumor, included in the World Health Organization (WHO) 2016 classification. Very few cases have been described in the literature so far, especially the infantile type. It is a mesenchymal tumor of the fibroblastic type, characterized by the fusion of NAB 2 and STAT 6 genes. A 10-month-old boy presented to our neurosurgery department with complaints of increasing head circumference since 1 month of age. The magnetic resonance imaging (MRI) showed a space-occupying lesion measuring 8.2 cm × 7 cm × 6.9 cm in the fronto-temporo-parietal region with a clinical diagnosis of glioma/atypical teratoid rhabdoid tumor (ATRT). The microscopy revealed a spindle cell tumor arranged in a patternless pattern with variable cellularity, increased mitosis, and areas of coagulative necrosis. The immunohistochemistry showed vimentin, CD 34, STAT6, CD99 positivity whereas Glial fibrillary acidic protein, Epithelial membrane antigen, and S-100 negativity. Hence, a diagnosis of anaplastic SFT/HPC (grade-III) was rendered. The patient improved after gross total resection (GTR). The primary intracranial congenital SFT/HPC are extremely rare, often a clinico-radiologically misdiagnosed entity. Thus, the immunohistochemistry/molecular study in addition to histology is mandatory for accurate diagnosis.","PeriodicalId":13488,"journal":{"name":"Indian Journal of Pathology and Microbiology","volume":"66 3","pages":"597-600"},"PeriodicalIF":1.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9975248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histological risk score and its role in predicting recurrence in early-stage oral squamous cell carcinomas. 早期口腔鳞状细胞癌的组织学风险评分及其在预测复发中的作用。

IF 1 4区医学

Indian Journal of Pathology and Microbiology Pub Date : 2023-07-01 DOI: 10.4103/ijpm.ijpm_514_21

Daphne Fonseca, Rashmi Khemani, Mohan K Pasam, Ravindranath Tagore, B Vishal Rao, Suseela Kodandapani, Chandrasekhara Rao, K V V N Raju, T Subramanyeshwar Rao

{"title":"Histological risk score and its role in predicting recurrence in early-stage oral squamous cell carcinomas.","authors":"Daphne Fonseca, Rashmi Khemani, Mohan K Pasam, Ravindranath Tagore, B Vishal Rao, Suseela Kodandapani, Chandrasekhara Rao, K V V N Raju, T Subramanyeshwar Rao","doi":"10.4103/ijpm.ijpm_514_21","DOIUrl":"10.4103/ijpm.ijpm_514_21","url":null,"abstract":"Context: Oral squamous cell carcinoma (OSCC) comprises more than 90% of oral cancers and is the most common carcinoma affecting the oral cavity. Early stage T1/T2 OSCC have a heterogeneous prognosis and a significant number of patients develop loco regional recurrence (LRR) and have reduced disease free survival (DFS) with increased disease related mortality.Aims and objectives: To assess the impact of the three parameters used in Brandwein-Gensler risk model along with lympho-vascular invasion (LVI), depth of invasion (DOI) and lymph node metastases in predicting LRR in early stage OSCC.Materials and methods: This was a retrospective study on early stage T1/2 OSCC patients over a period of 2 years who received treatment by surgical resection and had follow-up data. LRR was assessed based on recurrence of OSCC at the initial site or in regional lymph nodes.Results: Out of 1135 OSCC cases during our study period a total of 207 cases befitted our inclusion criteria. Recurrence was noted in 113 (54.6%) cases. Univariate analysis identified LVI (P < 0.00001), DOI (P < 0.00001), nodal involvement (P < 0.00001), worst pattern of invasion (WPOI) (P < 0.00001), lymphocytic host response (LHR) (P = 0.004), perineural invasion (PNI) (P = 0.012) as strong statistically significant risk factors for LRR.Conclusion: Adequate assessment of simple parameters on routine H and E by incorporating Brandwein-Gensler histological risk scoring model at the initial presentation can help prognosticate and predict LRR and select patients for post-surgical adjuvant therapy.","PeriodicalId":13488,"journal":{"name":"Indian Journal of Pathology and Microbiology","volume":"66 3","pages":"444-448"},"PeriodicalIF":1.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10296217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stem cell markers: A guide to neoadjuvant therapy in breast carcinomas. 干细胞标志物：乳腺癌新辅助治疗指南。

IF 1 4区医学

Indian Journal of Pathology and Microbiology Pub Date : 2023-07-01 DOI: 10.4103/ijpm.ijpm_1274_21

Zuhal Gucin, Nur Buyukpinarbasili, Melin Ozgun Gecer, Yeliz Emine Ersoy, Haci Mehmet Turk, Seyma Yildiz, Direnc Ozlem Aksoy

{"title":"Stem cell markers: A guide to neoadjuvant therapy in breast carcinomas.","authors":"Zuhal Gucin, Nur Buyukpinarbasili, Melin Ozgun Gecer, Yeliz Emine Ersoy, Haci Mehmet Turk, Seyma Yildiz, Direnc Ozlem Aksoy","doi":"10.4103/ijpm.ijpm_1274_21","DOIUrl":"10.4103/ijpm.ijpm_1274_21","url":null,"abstract":"Aim: This study aims to investigate potential associations between the stem cell population and the degree of tumor regression in breast carcinomas treated with neoadjuvant therapy.Settings and design: The study included 92 patients with breast carcinoma who received neoadjuvant therapy. Tumor regression was defined based on Miller and Payne grading system. Patients with grade 1 or 2 regression on a 5-point scale were included in group 1 (n = 37), grade 3 regression in group 2 (n = 32), and grade 4 or 5 regression in group 3 (n = 23).Materials and methods: Immunohistochemical staining was performed on paraffin block sections of every case using CD44, CD24, CD29, CD133, ID4, and ALDH1 antibodies to detect stem cells.Statistical analysis used: IBM Statistical Package for the Social Sciences (SPSS), version 23.0 (IBM Corp., Armonk, NY, USA) software was used for statistical analyses, and a P value less than 0.05 was considered statistically significant.Results: Histologically high-grade tumors are more common in the near-complete/complete response group (P = 0.004). HER2-positive tumors were more common in the complete/near-complete response group (P = 0.054). Tumor cells positive for stem cell markers CD44 and CD24 were more common in the poor response group (P = 0.027 and P = 0.001, respectively). CD29 expression was reduced in the posttreatment residual tumor tissue in the near-complete/complete response group.Conclusion: High CD44 and CD24 expression may be a predictor of poor response/nonresponse to neoadjuvant therapy in breast carcinomas.Background: In recent years, stem cells have been defined as the main cell population responsible for resistance to anticancer therapies.","PeriodicalId":13488,"journal":{"name":"Indian Journal of Pathology and Microbiology","volume":"66 3","pages":"495-501"},"PeriodicalIF":1.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10296219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The prognostic effect of immunohistochemical staining rates in patients with non-muscle-invasive bladder cancer. 免疫组织化学染色率对非肌肉浸润性癌症患者预后的影响。

IF 1 4区医学

Indian Journal of Pathology and Microbiology Pub Date : 2023-07-01 DOI: 10.4103/ijpm.ijpm_1236_21

Aykut Demirci, Melike Ordu

{"title":"The prognostic effect of immunohistochemical staining rates in patients with non-muscle-invasive bladder cancer.","authors":"Aykut Demirci, Melike Ordu","doi":"10.4103/ijpm.ijpm_1236_21","DOIUrl":"10.4103/ijpm.ijpm_1236_21","url":null,"abstract":"Context: Despite the follow-up protocols developed in non-muscle-invasive bladder cancer patients, progression and recurrence could not be prevented.Aims: We aimed to investigate whether proteins such as OCT-4, CD47, p53, Ki-67, and Survivin, which increase in bladder cancer cells, can be used as prognostic markers for patients with non-muscle-invasive bladder cancer.Settings and design: The study included a total of 89 patients with newly diagnosed non-muscle-invasive bladder cancer between January 2015 and December 2020.Materials and methods: Levels of OCT-4, CD47, p53, Kİ-67, and Survivin proteins in cancer cells were determined with a semi-quantitative immunohistochemical experiment. Pathological data and survival rates were compared according to the staining rates.Statistical analysis used: Data obtained in the study were analyzed statistically with SPSS 22.0 (SPSS, Chicago, IL, USA).Results: The mean age of the patients was 64.25 ± 9.91 years, and the median follow-up period was 55 months. Recurrence rate was determined to be 36% (n = 32), and the rate of progression at 40.4% (n = 36). The staining rates were stronger for each marker in the progression group and advanced-stage tumors (p < 0.001). The findings of the multivariate analysis carried out as part of the study showed that older age and higher tumor stage were independent risk factors for recurrence-free survival (HR = 1.048 and 7.074, respectively; P = 0.02). Also, higher tumor stages, diameters, and grades were associated with reduced progression-free survival (HR = 0.105, 0.395, 0.225, respectively; P < 0.05).Conclusions: Although immunohistochemical staining rates are promising, it is more appropriate to use tumor characteristics when assessing survival rate in patients with non-muscle-invasive bladder cancer.","PeriodicalId":13488,"journal":{"name":"Indian Journal of Pathology and Microbiology","volume":"66 3","pages":"502-510"},"PeriodicalIF":1.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10296220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0