Immunology letters最新文献

{"title":"Th1 adjuvant ARNAX, in combination with radiation therapy, enhances tumor regression in mouse tumor-implant models.","authors":"Aya Miyazaki, Sumito Yoshida, Yohei Takeda, Utano Tomaru, Misako Matsumoto, Tsukasa Seya","doi":"10.1016/j.imlet.2024.106947","DOIUrl":"10.1016/j.imlet.2024.106947","url":null,"abstract":"<p><p>Radiation therapy (RT) rarely induces tumor regression at untreated metastatic sites, the so-called abscopal effect. A syngeneic tumor (EG7) transplanted into a Th1-dominant mouse strain (C57BL/6) regressed significantly on the treated side and less on the contralateral side after RT. Additional subcutaneous administration of ARNAX, a non-inflammatory adjuvant, further accelerated tumor regression on the untreated side. This suggests that ARNAX after RT significantly enhances the tumor regression effect on the irrelevant tumor. Based on this setting, we next observed similar tumor shrinkage after RT and ARNAX by transplanting syngeneic breast cancer tumors (4T1) into a Th2-dominant mouse strain (BALB/c). The results were as follows: 1. ARNAX enhanced RT-mediated tumor shrinkage comparable to polyI:C; 2. In the Th2 mouse strain, little tumor regression occurred on the untreated side compared to tumor regression on the treated side after RT alone; 3. RT+ARNAX treatment caused additive regression on the treated side and induced slight tumor regression on the untreated side; 4. PD-L1 antibody + RT combination therapy caused tumor regression and further induced additive regression with ARNAX; 5. The combination of RT and ARNAX reduced the number and volume of lung metastases compared to RT alone. However, tumor regression was not always accompanied by a significant prolongation of survival in the mice receiving our regimen and protocol (one 10Gy radiation and a single ARNAX treatment). In conclusion, RT therapy promoted abscopal tumor regression in both Th2 and Th1 models with the addition of the non-inflammatory adjuvant ARNAX.</p>","PeriodicalId":13413,"journal":{"name":"Immunology letters","volume":" ","pages":"106947"},"PeriodicalIF":3.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Could there be a relationship between type III hypersensitivity reactions and graft rejections?","authors":"Rebeca Alessi Tedeschi Pachega, Renata Vaz Voltareli, Graziela Garrido Mori","doi":"10.1016/j.imlet.2025.106975","DOIUrl":"https://doi.org/10.1016/j.imlet.2025.106975","url":null,"abstract":"","PeriodicalId":13413,"journal":{"name":"Immunology letters","volume":" ","pages":"106975"},"PeriodicalIF":3.3,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143038270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Post hoc analysis: 6 Months immunogenicity after third dose of BNT162b2 vs JNJ-78436735 After Two Doses of BNT162b2 vaccine in Solid Organ Transplant Recipients.","authors":"Yoichiro Natori, Eric Martin, Adela Mattiazzi, Leopoldo Arosemena, George William Burke, Mrudula R Munagala, Suresh Manickavel, Katherine Sota, Suresh Pallikkuth, Jessie Chen, Julia Bini, Jacques Simkins, Shweta Anjan, Rodrigo M Vianna, Giselle Guerra","doi":"10.1016/j.imlet.2024.106968","DOIUrl":"https://doi.org/10.1016/j.imlet.2024.106968","url":null,"abstract":"<p><strong>Introduction: </strong>In Solid Organ Transplant (SOT) recipients, due to immunosuppression, the immunogenicity after COVID-19 vaccination is suboptimal and its durability is unknown.</p><p><strong>Methods: </strong>We conducted a post-hoc analysis of a patient-blinded, single center, randomized controlled trial comparing BNT162b2 vs JNJ-78436735 as the third dose after two doses of BNT162b2 in adult SOT recipients with active graft to compare long-term immunogenicity.</p><p><strong>Results: </strong>Forty-one recipients were analyzed. Median IgG levels against SARS-CoV-2 at 6 months were 53,747 (range 949 - 657,558) and 7,632 (range 642 - 672,000) AU/ml for BNT162b2 vs JNJ-78436735, respectively (p=0.017). The median geometric mean fold increase ratio at 6 months was 37.2 (0.12-618.5) and 4.30 (0.1-204.2) for BNT162b2 vs JNJ-78436735, respectively (p<0.05). After two doses of BNT162b2, homologous approach with BNT162b2 achieved a superior immunogenicity compared to heterologous approach with JNJ-78436735.</p><p><strong>Conclusion: </strong>In this post hoc analysis, we report durability of specific IgG between two vaccine strategies and found no statistically significant difference between two groups. (Clinical Trial Registry: NCT05047640).</p>","PeriodicalId":13413,"journal":{"name":"Immunology letters","volume":" ","pages":"106968"},"PeriodicalIF":3.3,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142970511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic value of CD163⁺ macrophages in solid tumor malignancies: A scoping review.","authors":"Henriette Mathiesen, Kristian Juul-Madsen, Trine Tramm, Thomas Vorup-Jensen, Holger Jon Møller, Anders Etzerodt, Morten Nørgaard Andersen","doi":"10.1016/j.imlet.2025.106970","DOIUrl":"10.1016/j.imlet.2025.106970","url":null,"abstract":"<p><p>Tumor-associated macrophages (TAMs) play crucial roles in development and progression of malignant diseases. Notably, CD163⁺ TAMs likely perform specific pro-tumorigenic functions, suggesting that this subset may serve as both prognostic biomarkers and targets for future anti-cancer therapy. We conducted a scoping review to map the current knowledge on the prognostic role of CD163⁺ TAMs in the five most lethal cancers worldwide: Lung, colorectal, gastric, liver, and breast cancer. For all cancer types, most studies showed that high tumoral presence of CD163⁺ cells was associated with poor patient outcome, and this association was more frequently observed when CD163⁺ cells were measured at the tumor periphery compared to more central parts of the tumor. These results support that CD163⁺ TAMs represent a biomarker of poor patient outcome across a variety of solid tumors, and highlight the relevance of further investigations of CD163⁺ TAMs as targets of future immunotherapies.</p>","PeriodicalId":13413,"journal":{"name":"Immunology letters","volume":" ","pages":"106970"},"PeriodicalIF":3.3,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142948210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The benefits of Lactiplantibacillus plantarum: From immunomodulator to vaccine vector.","authors":"Joshua Tobias, Stefan Heinl, Kristina Dendinovic, Ajša Ramić, Anna Schmid, Catherine Daniel, Ursula Wiedermann","doi":"10.1016/j.imlet.2025.106971","DOIUrl":"https://doi.org/10.1016/j.imlet.2025.106971","url":null,"abstract":"<p><p>Probiotics have been increasingly recognized for positively influencing many aspects of human health. Lactiplantibacillus plantarum (L. plantarum), a non-pathogenic bacterium, previously known as Lactobacillus plantarum, is one of the lactic acid bacteria commonly used in fermentation. The probiotic properties of L. plantarum have highlighted its health benefits to humans when consumed in adequate amounts. L. plantarum strains primarily enter the body orally and alter intestinal microflora and modulate the immune responses in their host; thereby benefiting human health. Furthermore, the use of L. plantarum as vaccine vectors delivering mucosal antigens has been shown to be a promising strategy. These aspects, from Immunomodulation to vaccine delivery by L. plantarum in preclinical settings, are highlighted in this review. Along these lines, construction of a recombinant L. plantarum strain expressing a B cell multi-peptide, as a future vaccine to modulate immunity and confer anti-tumor effect by targeting Her-2/neu-overexpressing cancers in local and distal sites, is also presented and discussed.</p>","PeriodicalId":13413,"journal":{"name":"Immunology letters","volume":"272 ","pages":"106971"},"PeriodicalIF":3.3,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142962046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of glutaminase 1 reduces M1 macrophage polarization to protect against monocrotaline-induced pulmonary arterial hypertension.","authors":"Xing Chen, Lixiang Li, Yan Deng, Juan Liao, Hui Meng, Limei Liang, Jie Hu, Dongwei Xie, Guizi Liang","doi":"10.1016/j.imlet.2025.106974","DOIUrl":"https://doi.org/10.1016/j.imlet.2025.106974","url":null,"abstract":"<p><p>(1) BACKGROUND: Metabolic abnormalities and immune inflammation are key elements within pathogenesis of pulmonary arterial hypertension (PAH). And in PAH patients, aberrant glutamine metabolism has been observed; however, the function of glutaminase 1 (GLS1) in macrophage is still unknown. So we aims to investigate GLS1's impact upon macrophages in PAH. (2) METHODS: We firstly constructed an monocrotaline (MCT)-induced PAH rat model. Briefly, the PAH rats were treated with the GLS1 inhibitor BPTES, and various index were evaluated, including hemodynamics, right ventricular function, pulmonary vascular remodeling, macrophage markers, and glutamine metabolism. After that, we polarized bone marrow-derived macrophages (BMDMs) into M1 phenotype and then subjected to BPTES intervention. Finally, we assessed macrophage phenotype, inflammatory markers, and glutamine metabolism indicators, along with the impact of BMDM supernatant on the behavior of pulmonary arterial smooth muscle cells (PASMCs). (3) RESULTS: GLS1 was significantly upregulated in both PAH patients and rats. Treatment with the GLS1 inhibitor BPTES markedly improved pulmonary arterial pressure, right ventricular function, and pulmonary vascular remodeling in PAH rats, while inhibiting M1 macrophage polarization, NLRP3 activation, and the release of pro-inflammatory cytokines. This, in turn, alleviated the proliferation and migration of PASMCs induced by inflammatory stimuli. (4) CONCLUSION: We propose that targeting GLS1 to reduce M1 macrophage polarization and inflammatory responses may represent a promising therapeutic approach for PAH.</p>","PeriodicalId":13413,"journal":{"name":"Immunology letters","volume":"272 ","pages":"106974"},"PeriodicalIF":3.3,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143004571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut TLRs and IFN pathways mRNA expression and frequencies of CD4⁺T cell expressing CD38 or HLA-DR go in parallel during HIV immunopathogenesis.","authors":"Luca Maddaloni, Ginevra Bugani, Letizia Santinelli, Alessandro Lazzaro, Federica Frasca, Matteo Fracella, Giancarlo Ceccarelli, Claudio M Mastroianni, Carolina Scagnolari, Gabriella d'Ettorre","doi":"10.1016/j.imlet.2025.106973","DOIUrl":"https://doi.org/10.1016/j.imlet.2025.106973","url":null,"abstract":"<p><p>While much is known about the expression of interferon (IFN) pathways in the blood of people living with HIV (PLWH), their role in the intesinal tract has only recently been appreciated. The aim of this study was to evaluate gut mRNA expression levels of innate immune genes involved in the HIV-host interaction and their association with CD4⁺T cell immune activation in long-term HAART-experienced PLWH. PLWH had increased intestinal levels of TLR4, type I IFN (IFN-α2, IFN-α14, IFN-β) and IFNAR1 mRNAs, as well as increased frequencies of CD4⁺T lymphocytes expressing CD38 or HLA-DR compared to the healthy donors. Moreover, TLR4, TLR9, IRF3, IRF7 and IFN-α14 mRNA expression was positively correlated with the frequency of CD4⁺T cells expressing CD38 or HLA-DR. These findings suggest a dysregulation of innate immunity, particularly of the TLRs and IFN pathways in the gut of PLWH. Genes in these pathways also appear to correlate with the levels of CD4⁺T cell immune activation.</p>","PeriodicalId":13413,"journal":{"name":"Immunology letters","volume":"272 ","pages":"106973"},"PeriodicalIF":3.3,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143004517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"METTL3/YTHDC1 mediates up-regulation of lncRNA OGRU in an m6A-dependent manner involving in oxidative stress and inflammation of HG-induced Müller cells.","authors":"ShuHua Fu, QianQian Zhou, Xin Peng, YaoYun Hu, Jian Xiong, Fei Liu","doi":"10.1016/j.imlet.2025.106972","DOIUrl":"10.1016/j.imlet.2025.106972","url":null,"abstract":"<p><strong>Background: </strong>Diabetic retinopathy (DR) is a common complication of diabetes, which may cause visual disturbance and even loss of sight. Oxidative stress and inflammation are two crucial pathological factors of DR; however, their specific regulatory mechanisms in DR remain obscure.</p><p><strong>Methods: </strong>DR models were established in streptozotocin-challenged rats and high glucose (HG)-stimulated Müller cells. Western blotting and RT-qPCR were performed to determine target molecule levels. ROS release was evaluated by DCFH-DA staining, and the levels of MDA, GSH, SOD, and CAT were detected using commercial kits. The levels of proinflammatory factors (TNF-α, IL-1β, IL-6, MCP-1, and CXCL-1) were analyzed by RT-qPCR and ELISA. The subcellular localization of OGRU was observed by FISH. Molecular interaction was evaluated by RIP. M6A level was assessed by MeRIP and colorimetric quantification kit.</p><p><strong>Results: </strong>HG stimulation or diabetic stress resulted in an elevation in the overall m6A level, as well as expression level of methyltransferase-like 3 (METTL3) in the experimental models of DR. M6A writer METTL3 stabilized lncRNA OGRU via m6A modification. Functionally, METTL3 deficiency relieved HG-induced oxidative stress damage and inflammation in Müller cells. Rescue assays demonstrated that OGRU overexpression reversed METTL3 silencing-mediated protection against HG-stimulated Müller cells. Furthermore, YTH Domain-Containing Protein 1 (YTHDC1) coordinated with METTL3 to enhance OGRU stability in an m6A-dependent manner.</p><p><strong>Conclusion: </strong>METTL3-mediated m6A modification stabilized OGRU with assistance of YTHDC1, which led to oxidative stress and inflammation during DR progression. Targeting METTL3/YTHDC1/OGRU axis might be a potential therapeutic strategy for DR.</p>","PeriodicalId":13413,"journal":{"name":"Immunology letters","volume":" ","pages":"106972"},"PeriodicalIF":3.3,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142931702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does atopic dermatitis increase the risk of allergic contact dermatitis?","authors":"Xingbao Luan, Xiaomei Cui, Yuanyuan Jia, Zhaopeng Wang, Yuting Yang, Kunpeng Wang, Dan Luo","doi":"10.1016/j.imlet.2025.106969","DOIUrl":"10.1016/j.imlet.2025.106969","url":null,"abstract":"","PeriodicalId":13413,"journal":{"name":"Immunology letters","volume":" ","pages":"106969"},"PeriodicalIF":3.3,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142926997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"X-ray irradiation reduces ATP-dependent activation of NLRP3 inflammasome by inhibiting TWIK2 activity in macrophages.","authors":"Xiaofei Huang, Man Niu, Tianjing Sun, Mo Li, Xuheng Jiang, Haizhen Duan, Tianxi Zhang, Ji Zhang, Fangke Xie, Renjie Song, Anyong Yu","doi":"10.1016/j.imlet.2024.106967","DOIUrl":"10.1016/j.imlet.2024.106967","url":null,"abstract":"<p><strong>Background: </strong>The spleen, as the body's largest peripheral immune organ and a crucial source of circulating monocytes, plays a significant role in the acute inflammatory response of spleen-derived macrophages to diseases. Therefore, studying the impact and mechanism of X-ray irradiation on spleen-derived macrophages' inflammatory responses is of great importance.</p><p><strong>Method: </strong>Extracted and identified mice splenic macrophages were divided into four groups: control group, LPS and ATP co-stimulated non-irradiated group, LPS and ATP co-stimulated group irradiated after 6 h, and LPS and ATP co-stimulated group irradiated after 12 h In the LPS and ATP co-stimulated groups, LPS (1μg/ml) and ATP (5mmol/L) were added to establish an inflammatory model in mice splenic macrophages. The irradiated groups were exposed to a medical linear accelerator (Elekta Synergy), while the non-irradiated groups were placed under the light source for the same duration without irradiation. Protein extraction was performed in each group at 6 h and 12 h post-treatment for subsequent analysis using Western blot, ELISA, RT-qPCR and other relevant methods.</p><p><strong>Results: </strong>(1) Compared with the non-irradiated group, the cell activity in the groups irradiated for 6 h and 12 h at 8 Gy showed a significant increase (P<0.01). (2) In the LPS and ATP co-stimulated groups irradiated after 6 h and 12 h, the expression of NLRP3 mRNA and protein, IL-18 and IL-1β showed a notable decrease compared to the LPS and ATP co-stimulated non-irradiated group (P<0.05). Additionally, caspase-1 expression of caspase-1 mRNA and protein in the 12 h post-irradiation group also decreased considerably when compared with the LPS and ATP co-stimulated non-irradiated group (P < 0.05). In the groups irradiated after 6 h and 12 h, (3) there was a remarkable decrease in the expression of TWIK mRNA and TWIK2, (4) as well as Gq mRNA and protein, when compared to the LPS and ATP co-stimulated non-irradiated group (P < 0.05). Particularly, the 12 h post-irradiation group exhibited a notable reduction in PKC expression (P < 0.05).</p><p><strong>Conclusion: </strong>X-ray irradiation is capable of inhibiting the activation of ATP-dependent NLRP3 inflammasomes in splenic macrophages.</p>","PeriodicalId":13413,"journal":{"name":"Immunology letters","volume":" ","pages":"106967"},"PeriodicalIF":3.3,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142894028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}