Immunology letters最新文献_第10页

Combining local cryoablation with PD-L1 blockade synergistically eradicates established murine lung cancer by modulating mitochondrial in PD-1+CD8+ T cell 局部冷冻消融与PD-L1阻断相结合，通过调节PD-1+CD8+T细胞中的线粒体，协同根除小鼠癌症。

IF 4.4 4区医学

Immunology letters Pub Date : 2023-10-05 DOI: 10.1016/j.imlet.2023.10.002

Jia-Wei Zhai , Lei-lei Lv , Jia-juan Wu , Yao-xin Zhang , Yu Shen , Qiu-xia Qu , Cheng Chen

{"title":"Combining local cryoablation with PD-L1 blockade synergistically eradicates established murine lung cancer by modulating mitochondrial in PD-1+CD8+ T cell","authors":"Jia-Wei Zhai , Lei-lei Lv , Jia-juan Wu , Yao-xin Zhang , Yu Shen , Qiu-xia Qu , Cheng Chen","doi":"10.1016/j.imlet.2023.10.002","DOIUrl":"10.1016/j.imlet.2023.10.002","url":null,"abstract":"<div><p>Immune checkpoint blockade (ICB) has shown improvement in overall survival for lung cancer in clinical trials. However, monotherapies have limited efficacy in improving outcomes and benefit only a subset of patients. Combination therapies targeting multiple pathways can augment an immune response to improve survival further. Here, we demonstrate that combinatorial anti-PD-L1/cryoablation therapy generated a synergistic antitumor activity in the established lung cancer model. Importantly, it was observed that this favorable antitumor immune response comes predominantly from the PD-1+CD8+ T cells generated after the combination therapy, referred as improvement of IFN-γ production and mitochondrial metabolism, which resembled highly functional effectors CD8+ T cells. Notably, the cellular levels of mitochondrial reactive oxygen and mitochondria mass excessively coincided with alteration of IFN-γ secretion in PD-1+CD8+T cell subset. So far, anti-PD-L1/cryoablation therapy selectively derived the improvement of depolarized mitochondria in PD-1+CD8+T cell subset, subsequently rebuild the anti-tumor function of the exhausted CD8+ T cells. Collectively, there is considerable interest in anti-PD-L1 plus cryoablation combination therapy for patients with lung cancer, and defining the underlying mechanisms of the observed synergy.</p></div>","PeriodicalId":13413,"journal":{"name":"Immunology letters","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41137949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dextran sulfate sodium-induced gut microbiota dysbiosis aggravates liver injury in mice with S100-induced autoimmune hepatitis 葡聚糖硫酸钠诱导的肠道微生物群失调加重S100诱导的自身免疫性肝炎小鼠的肝损伤。

IF 4.4 4区医学

Immunology letters Pub Date : 2023-10-04 DOI: 10.1016/j.imlet.2023.10.001

Zi-Ying Wang , Ping-Ping Gao , Ling Li , Ting-Ting Chen , Nan Li , Meng Qi , Sheng-Nan Zhang , Ya-Ping Xu , Yu-Han Wang , Shi-Hao Zhang , Ling-Ling Zhang , Wei Wei , Min Du , Wu-Yi Sun

{"title":"Dextran sulfate sodium-induced gut microbiota dysbiosis aggravates liver injury in mice with S100-induced autoimmune hepatitis","authors":"Zi-Ying Wang , Ping-Ping Gao , Ling Li , Ting-Ting Chen , Nan Li , Meng Qi , Sheng-Nan Zhang , Ya-Ping Xu , Yu-Han Wang , Shi-Hao Zhang , Ling-Ling Zhang , Wei Wei , Min Du , Wu-Yi Sun","doi":"10.1016/j.imlet.2023.10.001","DOIUrl":"10.1016/j.imlet.2023.10.001","url":null,"abstract":"<div><p>Recently, the incidence of autoimmune hepatitis (AIH) has gradually increased, and the disease can eventually develop into cirrhosis or even hepatoma if left untreated. AIH patients are often characterized by gut microbiota dysbiosis, but whether gut microbiota dysbiosis contributes to the progression of AIH remains unclear. In this study, we investigate the role of gut microbiota dysbiosis in the occurrence and development of AIH in mice with dextran sulfate sodium salt (DSS) induced colitis. C57BL/6J mice were randomly divided into normal group, S100-induced AIH group, and DSS+S100 group (1 % DSS in the drinking water), and the experimental cycle lasted for four weeks. We demonstrate that DSS administration aggravates hepatic inflammation and disruption of the intestinal barrier, and significantly changes the composition of gut microbiota in S100-induced AIH mice, which are mainly characterized by increased abundance of pathogenic bacteria and decreased abundance of beneficial bacteria. These results suggest that DSS administration aggravates liver injury of S100-induced AIH, which may be due to DSS induced gut microbiota dysbiosis, leading to disruption of the intestinal barrier, and then, the microbiota translocate to the liver, aggravating hepatic inflammation.</p></div>","PeriodicalId":13413,"journal":{"name":"Immunology letters","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41110448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pre-infection antibody levels of vaccinated healthcare workers with SARS-CoV-2 breakthrough infection: A nested case-control study 接种疫苗的严重急性呼吸系统综合征冠状病毒2型突破性感染医护人员感染前抗体水平：一项嵌套病例对照研究。

IF 4.4 4区医学

Immunology letters Pub Date : 2023-10-01 DOI: 10.1016/j.imlet.2023.08.002

Sema Alp Çavuş , Muammer Çelik , Ahmet Furkan Süner , Irmak Güzel , Çağlar Irmak , Derya Çağlayan , Huriye Gamze Öztürk , Neslişah Şiyve , Özgür Appak , Elif Işık , Gül Ergör , Osman Alparslan Ergör , Yücel Demiral , Ayça Arzu Sayıner , Bülent Kılıç

{"title":"Pre-infection antibody levels of vaccinated healthcare workers with SARS-CoV-2 breakthrough infection: A nested case-control study","authors":"Sema Alp Çavuş , Muammer Çelik , Ahmet Furkan Süner , Irmak Güzel , Çağlar Irmak , Derya Çağlayan , Huriye Gamze Öztürk , Neslişah Şiyve , Özgür Appak , Elif Işık , Gül Ergör , Osman Alparslan Ergör , Yücel Demiral , Ayça Arzu Sayıner , Bülent Kılıç","doi":"10.1016/j.imlet.2023.08.002","DOIUrl":"10.1016/j.imlet.2023.08.002","url":null,"abstract":"<div><h3>Aim</h3><p>To evaluate anti-RBD IgG antibody levels and neutralizing antibody titers between the health care workers (HCWs) with breakthrough SARS-CoV-2 infection and controls.</p></div><div><h3>Methods</h3><p>In this nested case-case control study, we followed 548 vaccinated HCWs with homologous (only with inactivated vaccine) or heterologous (both with inactivated and BNT162b2 vaccine) vaccination for 11 months, prospectively. We obtained blood samples from the participants for quantitative anti-RBD IgG and surrogate neutralization test. The participants with SARS-CoV-2 PCR positivity (at least 14 days after the last vaccination) were considered breakthrough infection. We chose 1:2 matched controls from the cohort, according to age, sex and vaccination status. We used R version 4.0.2 for the statistical analysis.</p></div><div><h3>Results</h3><p>Sixty-five cases and 130 controls were included in the study. The number of the breakthrough infections in HCWs were correlated with the pandemic waves in Türkiye and peaked during Omicron outbreak. The median age of the cases was 39 and 78.5% were female. The cases had more comorbidities than controls, significantly (<em>p</em> = 0.021). All cases experienced no or mild symptoms and recovered completely. Both pre-infection anti-RBD antibody and neutralizing antibody titers did not differ between cases and matched controls (<em>p</em> = 0.767, <em>p</em> = 0.628).</p></div><div><h3>Conclusion</h3><p>In this study, we showed that there was no comparable difference in humoral response after homologous or heterologous vaccination between the cases of breakthrough infection and matched controls. Compliance with infection control measures should be ensured, in combination with vaccination.</p></div>","PeriodicalId":13413,"journal":{"name":"Immunology letters","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10304400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced immunoprotection against Acinetobacter baumannii infection: Synergistic effects of Bap and BauA in a murine model 增强对鲍曼不动杆菌感染的免疫保护：Bap和BauA在小鼠模型中的协同作用。

IF 4.4 4区医学

Immunology letters Pub Date : 2023-10-01 DOI: 10.1016/j.imlet.2023.08.004

Mobina Mansouri , Masoomeh Sadeghpoor , Abolfazl Jahangiri , Mohammad Hossein Ghaini , Iraj Rasooli

{"title":"Enhanced immunoprotection against Acinetobacter baumannii infection: Synergistic effects of Bap and BauA in a murine model","authors":"Mobina Mansouri , Masoomeh Sadeghpoor , Abolfazl Jahangiri , Mohammad Hossein Ghaini , Iraj Rasooli","doi":"10.1016/j.imlet.2023.08.004","DOIUrl":"10.1016/j.imlet.2023.08.004","url":null,"abstract":"<div><h3>Background</h3><p>The rise of multi-drug resistant <em>Acinetobacter baumannii</em> poses a grave threat to hospital settings, resulting in increased mortality rates and garnering global attention. The formation of biofilms facilitated by biofilm-associated protein (Bap) and the iron absorption capabilities mediated by Baumannii acinetobactin utilization A (BauA) contribute to the persistence and survival of multidrug-resistant strains. In this study, we aimed to investigate the potential of disrupting the function of BauA and Bap simultaneously as a strategy for controlling <em>A. baumannii</em>.</p></div><div><h3>Methods</h3><p>Recombinant Bap and BauA were expressed, purified, and subcutaneously administered individually and in combination to BALB/c mice. Subsequently, mice were intraperitoneally challenged with <em>A. baumannii</em>, and the bacterial load and tissue damage in the spleen, lung, and liver were assessed. Serum samples were evaluated to determine antibody titers in surviving mice.</p></div><div><h3>Results</h3><p>Specific IgG antibodies were significantly increased. A combination of the antigens resulted in enhanced titer of specific IgGs in comparison to either BauA or Bap alone. The antibodies remained stable over a seven-month period. The combination of Bap and BauA exhibited superior immunoprotection against <em>A. baumannii</em> infection compared to individual administration, resulting in a further reduction in bacterial load in the liver, spleen, and lungs. The histopathological analysis demonstrated successful protection of the tissues against <em>A. baumannii</em>-induced damage upon administration of the two immunogens.</p></div><div><h3>Conclusions</h3><p>The combination of Bap and BauA has the potential to target a broader range of <em>A. baumannii</em> strains, including those expressing either Bap or BauA, thereby increasing its efficacy against a diverse array of strains.</p></div>","PeriodicalId":13413,"journal":{"name":"Immunology letters","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10307669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leveraging microRNAs for cellular therapy 利用微小RNA进行细胞治疗。

IF 4.4 4区医学

Immunology letters Pub Date : 2023-10-01 DOI: 10.1016/j.imlet.2023.08.005

Marko Hasiuk , Marianne Dölz , Romina Marone , Lukas T. Jeker

引用次数: 1

Rho-kinase inhibitors to deplete age-associated B cells in systemic autoimmunity Rho激酶抑制剂在系统性自身免疫中消耗与年龄相关的B细胞。

IF 4.4 4区医学

Immunology letters Pub Date : 2023-10-01 DOI: 10.1016/j.imlet.2023.09.004

Athanasios Sachinidis, Alexandros Garyfallos

引用次数: 0

Bothrops jararacussu snake venom decreases CD1d, CD83, and CD86 expression on bone marrow-derived dendritic cells 贾拉库苏蛇毒素降低骨髓来源的树突状细胞CD1d、CD83和CD86的表达。

IF 4.4 4区医学

Immunology letters Pub Date : 2023-10-01 DOI: 10.1016/j.imlet.2023.08.003

N.M. Nery , H.M. Santana , C.M.A. Rego , J.A. Lopes , M.D.S. Silva , A.A. Ferreira e Ferreira , V.P. Reis , M.V. Paloschi , S.N. Serrath , J.S.F. Bastos , C.P. Silva , J.G.S. Magalhães , L.F. Cruz , S.S. Setubal , J.P. Zuliani

{"title":"Bothrops jararacussu snake venom decreases CD1d, CD83, and CD86 expression on bone marrow-derived dendritic cells","authors":"N.M. Nery , H.M. Santana , C.M.A. Rego , J.A. Lopes , M.D.S. Silva , A.A. Ferreira e Ferreira , V.P. Reis , M.V. Paloschi , S.N. Serrath , J.S.F. Bastos , C.P. Silva , J.G.S. Magalhães , L.F. Cruz , S.S. Setubal , J.P. Zuliani","doi":"10.1016/j.imlet.2023.08.003","DOIUrl":"10.1016/j.imlet.2023.08.003","url":null,"abstract":"<div><p>This study was designed to characterize mice bone marrow (BM) and bone marrow-derived dendritic cells (BMDC) and to compare the surface markers expression and inflammatory cytokine liberation in response to LPS and <em>Bothrops jararacussu</em> venom (BjV) stimulation. Typical morphology was observed in BM and BMDCs from the 4th up to the 8th day of culture using recombinant mouse GM-CSF and IL-4. A high basal level of MHC-II, CD1d, CD83, CD11c, CD80, and low CD86 was expressed by BM cells. After stimulation with GM-CSF/IL-4 for BMDCs differentiation, the BM cells differentiated into BMDCs presented MHC-II, CD1d, CD83, CD11c, CD86, and CD80 expression on the 4th – 8th day accompanied with high levels of TNF-α liberated. The difference between the surface markers' expression was observed in this time course in which CD1d, CD11c, and CD80 remained in high levels of expression, while MHC-II and CD83 showed moderate expression during the differentiation period. Also, cytokines liberation was monitored over the period of the BMDCs culture, and on the 6th day, low levels of IL-6 and IL-1β were found, while high levels of TNF-α on the 4th and 8th days, both of which contributed to the maturity of the BMDCs. Maturation of DCs with LPS showed significant upregulation of surface markers (MHC-II, CD1d, CD83, CD86, CD80) and pro-inflammatory cytokines (IL-1β, IL-6, TNF-α) liberation. On the other hand, BjV induced a decrease in CD1d, CD11c, CD83, and CD86 expression in mature BMDCs which was not observed when LPS was used to stimulate BMDCs which probably induces impairment in T-cell activation.</p></div>","PeriodicalId":13413,"journal":{"name":"Immunology letters","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10304930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immune dysregulation as a leading principle for lymphoma development in diverse immunological backgrounds 免疫失调是不同免疫学背景下淋巴瘤发展的主要原则。

IF 4.4 4区医学

Immunology letters Pub Date : 2023-09-27 DOI: 10.1016/j.imlet.2023.08.007

P. Martijn Kolijn, Anton W. Langerak

{"title":"Immune dysregulation as a leading principle for lymphoma development in diverse immunological backgrounds","authors":"P. Martijn Kolijn, Anton W. Langerak","doi":"10.1016/j.imlet.2023.08.007","DOIUrl":"10.1016/j.imlet.2023.08.007","url":null,"abstract":"<div><p>Lymphoma is a heterogeneous group of malignancies arising from lymphocytes, which poses a significant challenge in terms of diagnosis and treatment due to its diverse subtypes and underlying mechanisms. This review aims to explore the shared and distinct features of various forms of lymphoma predisposing conditions, with a focus on genetic, immunological and molecular aspects. While diseases such as autoimmune disorders, inborn errors of immunity and iatrogenic immunodeficiencies are biologically and immunologically distinct, each of these diseases results in profound immune dysregulation and a predisposition to lymphoma development. Interestingly, the increased risk is often skewed towards a particular subtype of lymphoma. Patients with inborn errors of immunity in particular present with extreme forms of lymphoma predisposition, providing a unique opportunity to study the underlying mechanisms. External factors such as chronic infections and environmental exposures further modulate the risk of lymphoma development. Common features of conditions predisposing to lymphoma include: persistent inflammation, recurrent DNA damage or malfunctioning DNA repair, impaired tumor surveillance and viral clearance, and dysregulation of fundamental cellular processes such as activation, proliferation and apoptosis. Our growing understanding of the underlying mechanisms of lymphomagenesis provides opportunities for early detection, prevention and tailored treatment of lymphoma development.</p></div>","PeriodicalId":13413,"journal":{"name":"Immunology letters","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2023-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41102190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Vitamin A and vitamin D induced nuclear hormone receptor activation and its impact on B cell differentiation and immunoglobulin production 维生素A和维生素D诱导的核激素受体激活及其对B细胞分化和免疫球蛋白产生的影响。

IF 4.4 4区医学

Immunology letters Pub Date : 2023-09-27 DOI: 10.1016/j.imlet.2023.08.006

Padmavathy Ramanarayanan , Guido Heine , Margitta Worm

{"title":"Vitamin A and vitamin D induced nuclear hormone receptor activation and its impact on B cell differentiation and immunoglobulin production","authors":"Padmavathy Ramanarayanan , Guido Heine , Margitta Worm","doi":"10.1016/j.imlet.2023.08.006","DOIUrl":"10.1016/j.imlet.2023.08.006","url":null,"abstract":"<div><p>Vitamin A and vitamin D metabolites are ligands to nuclear receptors – namely RAR, RXR and VDR. The activation of these receptors in human B cells impacts B cell maturation and function. In this review, we discuss how 9-<em>cis</em> retinoic acid (9cRA) and 1,25-dihydroxyvitamin D3 (calcitriol) individually or in conjunction, signal through their nuclear receptors and thereby impact B cell differentiation, immunoglobulin class switching to IgA at the expense of IgE, and also B cell migration and homing. Impact of the vitamin metabolites individually on B cell survival factors are well elucidated, be it the regulation of BAFF and APRIL, the induction of TGF-β or suppression of NF-κB. Very little is known about the impact of 9cRA and calcitriol together on B cells. Recently our group revealed that 9cRA and calcitriol together in the context of the B cell differentiation, induces naïve B cell differentiation into IgA<sup>+</sup> plasmablasts, the functional and underlying molecular regulations however require further investigation. In conclusion, the conjunctional impact of these nuclear receptor ligands on B cell functionality is important to better understand B cell dependent clinical outcomes in allergy and autoimmunity. Within this review, we hypothesize that a balance between both vitamins is of utmost importance to provide a robust humoral immune response and a better treatment of disorders characterised by dysregulated immune responses such as IgE-dependent allergy or autoimmunity such as lupus erythematosus.</p></div>","PeriodicalId":13413,"journal":{"name":"Immunology letters","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2023-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41128212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Pentavalent outer membrane vesicles immunized mice sera confers passive protection against five prevalent pathotypes of diarrhoeagenic Escherichia coli in neonatal mice 五价外膜囊泡免疫小鼠血清对新生小鼠腹泻性大肠杆菌的五种常见病理类型具有被动保护作用。

IF 4.4 4区医学

Immunology letters Pub Date : 2023-09-19 DOI: 10.1016/j.imlet.2023.09.009

Soumalya Banerjee , Prolay Halder , Sanjib Das , Suhrid Maiti , Ushasi Bhaumik , Moumita Dutta , Goutam Chowdhury , Kei Kitahara , Shin-ichi Miyoshi , Asish Kumar Mukhopadhyay , Shanta Dutta , Hemanta Koley

{"title":"Pentavalent outer membrane vesicles immunized mice sera confers passive protection against five prevalent pathotypes of diarrhoeagenic Escherichia coli in neonatal mice","authors":"Soumalya Banerjee , Prolay Halder , Sanjib Das , Suhrid Maiti , Ushasi Bhaumik , Moumita Dutta , Goutam Chowdhury , Kei Kitahara , Shin-ichi Miyoshi , Asish Kumar Mukhopadhyay , Shanta Dutta , Hemanta Koley","doi":"10.1016/j.imlet.2023.09.009","DOIUrl":"10.1016/j.imlet.2023.09.009","url":null,"abstract":"<div><p>Diarrhoeagenic <em>Escherichia coli</em> (DEC) pathotypes are one of the major causative agents of diarrhoea induced childhood morbidity and mortality in developing countries. Licensed vaccines providing broad spectrum protection against DEC mediated infections are not available. Outer membrane vesicles (OMVs) are microvesicles released by gram-negative bacteria during the growth phase and contain multiple immunogenic proteins. Based on prevalence of infections, we have formulated a pentavalent outer-membrane vesicles (POMVs) based immunogen targeting five main pathotypes of DEC responsible for diarrhoeal diseases. Following isolation, OMVs from five DEC pathotypes were mixed in equal proportions to formulate POMVs and 10 µg of the immunogen was intraperitoneally administered to adult BALB/c mice. Three doses of POMVs induced significant humoral immune response against whole cell lysates (WCLs), outer membrane proteins (OMPs) and lipopolysaccharides (LPS) isolated from DEC pathotypes along with significant induction of cellular immune response in adult mice. Passive transfer of POMVs immunized adult mice sera protected neonatal mice significantly against DEC infections. Overall, this study finds POMVs to be immunogenic in conferring broad-spectrum passive protection to neonatal mice against five main DEC pathotypes. Altogether, these findings suggest that POMVs can be used as a potent vaccine candidate to ameliorate the DEC-mediated health burden.</p></div>","PeriodicalId":13413,"journal":{"name":"Immunology letters","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41128213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0