Immunologic Research最新文献_第9页

Evolutionary preservation of CpG dinucleotides in RAG1 may elucidate the relatively high rate of methylation-mediated mutagenesis of RAG1 transposase. RAG1 中 CpG 二核苷酸的进化保存可以解释 RAG1 转座酶甲基化介导的诱变率相对较高的原因。

IF 3.3 4区医学

Immunologic Research Pub Date : 2024-06-01 Epub Date: 2024-01-19 DOI: 10.1007/s12026-023-09451-8

Mariam M Fawzy, Maiiada H Nazmy, Azza A K El-Sheikh, Moustafa Fathy

{"title":"Evolutionary preservation of CpG dinucleotides in RAG1 may elucidate the relatively high rate of methylation-mediated mutagenesis of RAG1 transposase.","authors":"Mariam M Fawzy, Maiiada H Nazmy, Azza A K El-Sheikh, Moustafa Fathy","doi":"10.1007/s12026-023-09451-8","DOIUrl":"10.1007/s12026-023-09451-8","url":null,"abstract":"Recombination-activating gene 1 (RAG1) is a vital player in V(D)J recombination, a fundamental process in primary B cell and T cell receptor diversification of the adaptive immune system. Current vertebrate RAG evolved from RAG transposon; however, it has been modified to play a crucial role in the adaptive system instead of being irreversibly silenced by CpG methylation. By interrogating a range of publicly available datasets, the current study investigated whether RAG1 has retained a disproportionate level of its original CpG dinucleotides compared to other genes, thereby rendering it more exposed to methylation-mediated mutation. Here, we show that 57.57% of RAG1 pathogenic mutations and 51.6% of RAG1 disease-causing mutations were associated with CpG methylation, a percentage that was significantly higher than that of its RAG2 cofactor alongside the whole genome. The CpG scores and densities for all RAG ancestors suggested that RAG transposon was CpG denser. The percentage of the ancestral CpG of RAG1 and RAG2 were 6% and 4.2%, respectively, with no preference towards CG containing codons. Furthermore, CpG loci of RAG1 in sperms were significantly higher methylated than that of RAG2. In conclusion, RAG1 has been exposed to CpG mediated methylation mutagenesis more than RAG2 and the whole genome, presumably due to its late entry to the genome later with an initially higher CpG content.","PeriodicalId":13389,"journal":{"name":"Immunologic Research","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11217092/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139491106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rituximab alleviates pediatric systemic lupus erythematosus associated refractory immune thrombocytopenia: a case-based review. 利妥昔单抗缓解小儿系统性红斑狼疮相关难治性免疫血小板减少症：基于病例的综述。

IF 3.3 4区医学

Immunologic Research Pub Date : 2024-06-01 Epub Date: 2024-01-27 DOI: 10.1007/s12026-024-09454-z

Fangxin Mu, Xue Bai, Yan Lou, Ping Luo, Qiaoyan Guo

引用次数: 0

Correction to: Characteristics of splenic PD-1⁺ γδT cells in Plasmodium yoelii nigeriensis infection. 更正：尼日尔疟原虫感染中脾脏 PD-1+ γδT 细胞的特征。

IF 3.3 4区医学

Immunologic Research Pub Date : 2024-06-01 DOI: 10.1007/s12026-024-09469-6

Dianhui Chen, Feng Mo, Meiling Liu, Lin Liu, Junmin Xing, Wei Xiao, Yumei Gong, Shanni Tang, Zhengrong Tan, Guikuan Liang, Hongyan Xie, Jun Huang, Juan Shen, Xingfei Pan

引用次数: 0

Response to: regarding the significance of anti-COVID-IgA antibody response in COVID-19 breakthrough infection. 回复：关于 COVID-19 突破性感染中抗 COVID-IgA 抗体反应的意义。

IF 3.3 4区医学

Immunologic Research Pub Date : 2024-06-01 Epub Date: 2024-01-05 DOI: 10.1007/s12026-023-09452-7

Sabiha Anis, Mariam Ashfaq Khan, Areej Fatima, Fatima Kanani, Javeria Aijaz, Aneela Hussain, Samreen Sarfaraz

{"title":"Response to: regarding the significance of anti-COVID-IgA antibody response in COVID-19 breakthrough infection.","authors":"Sabiha Anis, Mariam Ashfaq Khan, Areej Fatima, Fatima Kanani, Javeria Aijaz, Aneela Hussain, Samreen Sarfaraz","doi":"10.1007/s12026-023-09452-7","DOIUrl":"10.1007/s12026-023-09452-7","url":null,"abstract":"In response to Chen et al.'s comments on our paper regarding the significance of anti-COVID-IgA antibody response in COVID-19 breakthrough infection in vaccinated patients, we have highlighted the role and the scope of this paper in this correspondence. The role of anti-COVID-19-IgA is already known. The objective of the previous study was to see its role in breakthrough-infected patients. To analyse this effect, we recruited patients with COVID-19 infection after they were fully vaccinated and compared them with the vaccinated group who did not get the infection. Both groups were equally exposed to the virus as all of them were health care workers. We also showed that the anti-COVID-19-NP-IgA was absent in the healthy cohort of our study groups, signifying the absence of natural infection in them during this period. The article also highlights the importance of vaccinating all individuals including those who are immunosuppressed, as it prevents severe COVID-19 infection in these individuals. The physicians should be aware of the fact that immunosuppressed patients are more likely to get COVID-19 breakthrough infection. However, proper vaccination with booster doses prevents severe infection in them.","PeriodicalId":13389,"journal":{"name":"Immunologic Research","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139097757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum soluble LYVE1 is a promising non-invasive biomarker of renal fibrosis: a population-based retrospective cross-sectional study. 血清可溶性 LYVE1 是一种很有前景的肾脏纤维化非侵入性生物标记物：一项基于人群的回顾性横断面研究。

IF 3.3 4区医学

Immunologic Research Pub Date : 2024-06-01 Epub Date: 2023-12-23 DOI: 10.1007/s12026-023-09448-3

Jing Liu, Yuqing Liu, Wenqian Zhou, Yiguo Liu, Saiya Zhu, Ying Yu, Jieli Huang, Chen Yu

{"title":"Serum soluble LYVE1 is a promising non-invasive biomarker of renal fibrosis: a population-based retrospective cross-sectional study.","authors":"Jing Liu, Yuqing Liu, Wenqian Zhou, Yiguo Liu, Saiya Zhu, Ying Yu, Jieli Huang, Chen Yu","doi":"10.1007/s12026-023-09448-3","DOIUrl":"10.1007/s12026-023-09448-3","url":null,"abstract":"Diagnosis of renal fibrosis can only be verified by kidney biopsy, but biomarkers for non-invasive evaluation remain unsatisfactory. Patients with fibrosis often have abnormalities of the lymphatic vascular system and associated immune function. We describe here a lymphatic marker as a candidate biomarker for fibrosis. After assessing and grading the fibrosis scores, testing serum soluble lymphatic vessel endothelial hyaluronan receptor1 (sLYVE1) level, and collecting clinical information, the association between sLYVE1 and renal fibrosis was analyzed. Logistic regression analysis was used to screen variables. Diagnosis models with or without sLYVE1 were built, and nomograms were plotted. Calibration curve, C-index, and DCA were performed to assess the models. A total of 298 patients were enrolled in the study, of which 199 were included in the training cohort and 99 patients in the validation cohort. Serum sLYVE1 levels markedly elevated with increasing fibrosis grade (p<0.05). ROC analysis of sLYVE1 showed an AUC of 0.791 and 0.846 with optimal cut-off value of 405.25 ng/mL and 498.55 ng/mL for the prediction of moderate-to-severe renal fibrosis (MSF) and severe renal fibrosis (SF), respectively. The diagnostic nomogram model without sLYVE1 (model 1) included traditional clinical determinants (C-index: 0.658 for MSF; 0.603 for SF). A combination of model 1 and sLYVE1 (model 2) improved predictive performance (C-index: 0.847 for MSF; 0.856 for SF). Calibration curve and DCA demonstrated a better consistency accuracy and clinical benefit of model 2 than model 1. Serum sLYVE1 may be identified as a potential biomarker of renal fibrosis. Models incorporating sLYVE1 may be beneficial for a more accurate non-invasive diagnosis of renal fibrosis.","PeriodicalId":13389,"journal":{"name":"Immunologic Research","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11217098/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138884892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CCR2+TREM-1+ monocytes promote natural killer T cell dysfunction contributing towards HBV disease progression. CCR2+TREM-1+单核细胞促进自然杀伤 T 细胞功能失调，导致 HBV 疾病进展。

IF 4.4 4区医学

Immunologic Research Pub Date : 2024-05-30 DOI: 10.1007/s12026-024-09495-4

Xiaojuan Wu, Wenling Zhao, Qiang Miao, Shiya Shi, Bin Wei, Limei Luo, Bei Cai

{"title":"CCR2+TREM-1+ monocytes promote natural killer T cell dysfunction contributing towards HBV disease progression.","authors":"Xiaojuan Wu, Wenling Zhao, Qiang Miao, Shiya Shi, Bin Wei, Limei Luo, Bei Cai","doi":"10.1007/s12026-024-09495-4","DOIUrl":"https://doi.org/10.1007/s12026-024-09495-4","url":null,"abstract":"Natural killer T (NKT) cells are amongst the most important innate immune cells against hepatitis B virus (HBV) infection. Moreover, previous studies have shown that HBV infection induced TREM-1+ expression in monocyte and secretion of inflammatory cytokines. Thus, this prompted us to elucidate the role of TREM-1+ monocytes in regulating the function of iNKT cells. Ninety patients and 20 healthy participants were enrolled in the study. The percentage and phenotype of iNKT cells and TREM-1+ monocytes were measured in the peripheral blood of healthy controls (HC), patients with chronic HBV infection (CHB), HBV-related liver cirrhosis (LC), and HBV-related acute-on-chronic liver failure (ACLF) via flow cytometry. Moreover, co-culture experiments with iNKT cells and TREM-1 overexpressing THP-1 cells were performed to determine the role of TREM-1 in the regulation of NKT cell function. We observed that the percentage of iNKT cells and CD4-iNKT cells gradually decreased, whereas the percentage of CCR2+TREM-1+ monocytes increased with the progression of the disease. In addition, activation of the TREM-1 signaling pathway induced the secretion of inflammatory cytokines leading to pyroptosis of iNKT cells and secretion of IL-17 contributing towards disease progression. Therefore, this study suggests that blocking the activation of TREM-1 in monocytes could promote the elimination of HBV by inhibiting pyroptosis of iNKT cells and restoring their function. However, further studies are required to validate these results that would help in developing new treatment strategies for patients with HBV infections.","PeriodicalId":13389,"journal":{"name":"Immunologic Research","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141175479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of NLRP3 inflammasome activation in patients with chronic obstructive pulmonary disease before and after lung transplantation. 评估肺移植前后慢性阻塞性肺病患者体内 NLRP3 炎症小体的激活情况。

IF 4.4 4区医学

Immunologic Research Pub Date : 2024-05-29 DOI: 10.1007/s12026-024-09497-2

Lada Rumora, Ivona Markelić, Iva Hlapčić, Andrea Hulina Tomašković, Marija Fabijanec, Feđa Džubur, Miroslav Samaržija, Andrea Vukić Dugac

{"title":"Assessment of NLRP3 inflammasome activation in patients with chronic obstructive pulmonary disease before and after lung transplantation.","authors":"Lada Rumora, Ivona Markelić, Iva Hlapčić, Andrea Hulina Tomašković, Marija Fabijanec, Feđa Džubur, Miroslav Samaržija, Andrea Vukić Dugac","doi":"10.1007/s12026-024-09497-2","DOIUrl":"https://doi.org/10.1007/s12026-024-09497-2","url":null,"abstract":"The interplay between purinergic receptors as well as pattern recognition receptors like Toll-like receptors (TLRs) and NOD-, LRR-, and pyrin domain-containing protein 3 (NLRP3) might have a role in the pathogenesis of chronic obstructive pulmonary disease (COPD). The aim of this study was to determine and compare the concentrations of the damage-associated molecular patterns (DAMPs) heat shock protein 70 (Hsp70) and adenosine triphosphate (ATP), and gene expression of their respective receptors as well as NLRP3 inflammasome-related molecules in the peripheral blood of patients with end-stage COPD before and 1 year after lung transplantation (LT). Lung function was assessed by spirometry and diffusion capacity for carbon monoxide (DLCO). Quantitative polymerase chain reaction (qPCR) was applied for detection of TLR2, TLR4, P2X7R, P2Y2R, IL1B, CASP1, and NLRP3 expression. High-sensitivity ELISA kits were used for extracellular (e) Hsp70 and IL-1β, and luminescence assay for eATP measurements. Concentrations of eHsp70 and eATP as well as IL-1β were significantly increased in the plasma of end-stage COPD patients and significantly decreased after LT. In addition, TLR4, P2Y2R, IL1B, CASP1, and NLRP3 expression was up-regulated in COPD patients before LT, while it was significantly suppressed after LT. In conclusion, it could be assumed that NLRP3 inflammasome is activated in the peripheral blood of end-stage COPD patients and that eHsp70 and eATP could be responsible for its activation through triggering their receptors. On the other hand, previously enhanced pro-inflammatory reactions seem to be suppressed to the healthy population levels in lung recipients without allograft rejection.","PeriodicalId":13389,"journal":{"name":"Immunologic Research","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141175467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gender disparities in Behçet's syndrome: identifying distinct phenotypes through cluster analysis. 贝赫切特综合征的性别差异：通过聚类分析确定不同的表型。

IF 4.4 4区医学

Immunologic Research Pub Date : 2024-05-29 DOI: 10.1007/s12026-024-09498-1

Gamze Kılıç, Kemal Faruk Körüklü, Muhammed Galip Kumcu, Elif Çakır, Murat Karkucak, Erkan Kılıç

{"title":"Gender disparities in Behçet's syndrome: identifying distinct phenotypes through cluster analysis.","authors":"Gamze Kılıç, Kemal Faruk Körüklü, Muhammed Galip Kumcu, Elif Çakır, Murat Karkucak, Erkan Kılıç","doi":"10.1007/s12026-024-09498-1","DOIUrl":"https://doi.org/10.1007/s12026-024-09498-1","url":null,"abstract":"Behçet's syndrome (BS) is a complex, multi-systemic disorder with a global occurrence, notably concentrated along the Silk Road. This study aimed to investigate gender-specific expressions and clinical phenotypes in BS patients within the Eastern Black Sea Region of Turkey. A total of 290 BS patients were retrospectively analyzed between January 2013 and December 2023. Demographic characteristics, clinical manifestations, medical treatment, and pathergy test results were obtained from a review of medical records. The mean age was 45.79 ± 13.05, with a male-to-female ratio of 48.6:51.4. Male patients had higher papulopustular lesions (p < 0.001) and ocular involvement (p = 0.036), while females showed more frequent genital ulcers (p = 0.032). Medication usage showed gender-based variations, notably higher corticosteroid, azathioprine, and tumor necrosis factor-alpha inhibitor (TNFi) use in males (p < 0.001). Cluster analysis revealed five distinct clusters, each with unique features and gender predominance. Cardiovascular type, ocular type, and skin type predominantly featured male patients, while joint involvement type and neurologic and mucosal involvement type were more prevalent among female patients with BS. This research contributes valuable insights into the gender-related clinical variations of BS within a specific geographic region, fostering a more comprehensive understanding of this challenging syndrome. The identification of distinct clinical phenotypes facilitates the development of tailored treatment strategies, potentially leading to improved outcomes for patients with BS.","PeriodicalId":13389,"journal":{"name":"Immunologic Research","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141160899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The immunosuppressive landscape in tumor microenvironment 肿瘤微环境中的免疫抑制环境

IF 4.4 4区医学

Immunologic Research Pub Date : 2024-05-01 DOI: 10.1007/s12026-024-09483-8

Wuyi Liu, Huyue Zhou, Wenjing Lai, Changpeng Hu, Rufu Xu, Peng Gu, Menglin Luo, Rong Zhang, Guobing Li

引用次数: 0

SIT1 identifies circulating hypoactive T cells with elevated cytotoxic molecule secretion in systemic lupus erythematosus patients SIT1 可识别系统性红斑狼疮患者中细胞毒性分子分泌增加的循环低活性 T 细胞

IF 4.4 4区医学

Immunologic Research Pub Date : 2024-05-01 DOI: 10.1007/s12026-024-09481-w

Ainizati Hasimu, Ayibaota Bahabayi, Ziqi Xiong, Qi Li, Zhonghui Zhang, Xingyue Zeng, Mohan Zheng, Zihang Yuan, Chen Liu

{"title":"SIT1 identifies circulating hypoactive T cells with elevated cytotoxic molecule secretion in systemic lupus erythematosus patients","authors":"Ainizati Hasimu, Ayibaota Bahabayi, Ziqi Xiong, Qi Li, Zhonghui Zhang, Xingyue Zeng, Mohan Zheng, Zihang Yuan, Chen Liu","doi":"10.1007/s12026-024-09481-w","DOIUrl":"https://doi.org/10.1007/s12026-024-09481-w","url":null,"abstract":"This study aims to elucidate the expression and functionality of SIT1 in circulating CD8/CD4 + T cells in humans and to delineate its significance in systemic lupus erythematosus (SLE) patients. We employed multiparametric flow cytometry to investigate the expression of SIT1 in circulating CD8/CD4 + T cells and their respective subsets, comparing healthy controls (HCs) with SLE patients. Furthermore, we assessed the levels of granzyme B, perforin, IL-17, and IFN-γ in SIT1-related CD8/CD4 + T cells from both HCs and SLE patients, both before and after PMA stimulation. Clinically, we conducted receiver operating characteristic curve analysis and correlation analysis to evaluate the clinical relevance of SIT1-related CD8/CD4 + T cells in SLE patients. SIT1 exhibited higher expression in CD4 + T cells, with SIT1 − T cells demonstrating elevated levels of granzyme B, perforin, and IFN-γ compared to SIT1 + T cells. PMA-stimulated T cells exhibited reduced SIT1 expression compared to unstimulated T cells. SLE patients displayed increased SIT1 + proportions in CD8 + T cells and decreased SIT1 + CD4 + T cell numbers. Additionally, SIT1 + cells in SLE patients exhibited significantly higher levels of granzyme B and perforin compared to HCs. SIT1 + cells demonstrated significant associations with clinical indicators in SLE patients, with indicators related to SIT1 proving valuable in the diagnosis of SLE patients. SIT1 is inversely correlated with T cell activation. In SLE patients, SIT1 expression is altered in T cells concomitant with an augmented secretion of cytotoxic molecules. This upregulation may contribute to the pathogenesis of SLE and enhance its diagnostic potential.<h3 data-test=\"abstract-sub-heading\">Graphical abstract</h3>\u0000","PeriodicalId":13389,"journal":{"name":"Immunologic Research","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140834495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0