Indian Journal of Genetics and Plant Breeding最新文献_第6页

Identification of potential donor for pro-vitamin A using functional markers in maize (Zea mays L) 利用玉米(Zea mays L)功能标记鉴定维生素A原的潜在供体

IF 1 4区生物学

Indian Journal of Genetics and Plant Breeding Pub Date : 2021-03-25 DOI: 10.31742/ijgpb.81.1.5

A. Das, A. Singode, D. Chaudhary, Yathish Kr, Chikkappa Gangadhar Karjagi, Ramesh Kumar, B. Kumar, Vishal Singh, G. Mukri, Sapna, S. Rakshit

{"title":"Identification of potential donor for pro-vitamin A using functional markers in maize (Zea mays L)","authors":"A. Das, A. Singode, D. Chaudhary, Yathish Kr, Chikkappa Gangadhar Karjagi, Ramesh Kumar, B. Kumar, Vishal Singh, G. Mukri, Sapna, S. Rakshit","doi":"10.31742/ijgpb.81.1.5","DOIUrl":"https://doi.org/10.31742/ijgpb.81.1.5","url":null,"abstract":"Vitamin A deficiency (VAD) is a global health problem. Maize naturally accumulates carotenoids including ?-carotene, a major source of vitamin A. Thirty-seven Indian maize genotypes were screened for total carotenoids and ?carotene content. A positive significant association of kernel colour was observed with total carotenoid but not for ?-carotene. The level of ?-carotene among the selected 37 genotypes was low (0.14 to 4.63µg/g) as compared to total carotenoids (15.94 to 66.46 µg/g) thus necessitates the introduction and deployment of lines with high ?carotene. Hence, 54 inbred lines developed under the HarvestPlus programme were screened with functional markers of lcyE and crtRB1 genes of the carotenoid biosynthetic pathway. Three genotypes (HP704-13, HP704- 22, and HP704-23) amplified most of the favorable alleles for both these genes. However, due to long Anthesis Silking Interval (ASI) and poor adaptation HP704-13 and HP704-23 could not be maintained. Under normal storage condition genotype, HP704-22 recorded 7.32 µg/g of ?-carotene which can be used as a donor for provitamin-A or to diversify the Indian germplasm.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2021-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45120316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of chloroplast microsatellite markers in Capsicum: Insight into evolution of Bhut Jolokia - a clad of ghost chilli landraces 辣椒叶绿体微卫星标记的发展:鬼辣椒地方品种Bhut Jolokia的进化

IF 1 4区生物学

Indian Journal of Genetics and Plant Breeding Pub Date : 2021-03-25 DOI: 10.31742/ijgpb.81.1.10

A. Islam, S. Shivaraj, Prof Vikas Kumar, D. S. Phad, H. Sonah, S. B. Tripathi, R. Deshmukh

{"title":"Development of chloroplast microsatellite markers in Capsicum: Insight into evolution of Bhut Jolokia - a clad of ghost chilli landraces","authors":"A. Islam, S. Shivaraj, Prof Vikas Kumar, D. S. Phad, H. Sonah, S. B. Tripathi, R. Deshmukh","doi":"10.31742/ijgpb.81.1.10","DOIUrl":"https://doi.org/10.31742/ijgpb.81.1.10","url":null,"abstract":"In the present study, a total of 27 chloroplast specific SSRs (CpSSR) have been identified in the chloroplast genome of Capsicum annum L. The frequency of the SSRs was about one in 5.7 kb of the chloroplast genome. Out of 27 SSRs, 26 were mono-nucleotide repeats of A/T and one was a trinucleotide repeat (TTA). Further a set of seven markers were validated by genotyping 48 capsicum accessions comprising of cultivars from five different species and landraces of unknown identity. The seven SSR markers generated a total of 27 alleles among 48 samples used in this study. The size of the amplicons varied from 161 bp (CaCpM22 and 26) to 339 bp (CaCpM06). The polymorphic information content (PIC) value for the set of the primers used ranged from 0.11 to 0.48 with an average of 0.33. The number of alleles for markers ranged from three to six with an average of 3.28 alleles per marker. The phylogenetic analysis of the chilly accessions showed that the Bhut jolokia land race is clustered along with the C. frutescence indicating the it’s probable parentage. The chloroplast genome based SSR markers identified in the present study can be further used for the marker-assisted genomic studies","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2021-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41971811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New hyper-variable SSRs for diversity analysis in mango (Mangifera indica L.) 芒果多样性分析的新的超变量SSR

IF 1 4区生物学

Indian Journal of Genetics and Plant Breeding Pub Date : 2021-03-25 DOI: 10.31742/ijgpb.81.1.13

M. Srivastav, S. Singh, J. Prakash, Rakesh Singh, N. Sharma, Sridhar Ramchandra, Rajni Devi, Ankit Gupta, A. K. Mahto, P. Jayaswal, Sangeeta Singh, N. Singh

{"title":"New hyper-variable SSRs for diversity analysis in mango (Mangifera indica L.)","authors":"M. Srivastav, S. Singh, J. Prakash, Rakesh Singh, N. Sharma, Sridhar Ramchandra, Rajni Devi, Ankit Gupta, A. K. Mahto, P. Jayaswal, Sangeeta Singh, N. Singh","doi":"10.31742/ijgpb.81.1.13","DOIUrl":"https://doi.org/10.31742/ijgpb.81.1.13","url":null,"abstract":"Whole genome sequence in mango offers unprecedented opportunities for genomics assisted crop improvement via enabling access to genome-wide genetic markers. In the present study, simple sequence repeats (SSRs) were predicted from genome sequence of mango. Based on the SSR length (? 50 bp), highly-variable mango SSRs (MSSRs) were sorted. A sub-set of 129 MSSRs was validated on a set of 24 diverse mango genotypes yielding marker validation and polymorphism of 88.96 and 85.27 per cent, respectively. One hundred and ten polymorphic markers were identified for the present set of mango genotypes. Polymorphic information content (PIC) ranged from 0.10 to 0.78 and the highest value was observed with MSSR133. The mean PIC value was 0.40 but 33 MSSR markers showed PIC values ? 0.5, suggesting that these markers can efficiently measure genetic diversity and serve for mapping of quantitative trait loci (QTLs) in mango. MSSRs data was further used for diversity analysis of mango genotypes belonging to different agro-ecological conditions including chance seedlings, landraces, exotic and indigenous germplasm and hybrids. Cluster analysis using UPGMA of 24 mango genotypes revealed that these MSSRs were informative in diversity analysis and distinguished mango genotypes based on their origin, parentage and embryo types. A novel set of 110 hyper-variable SSR markers have been added to the mango repository depicting usefulness in discriminating closely related mango germplasm and their use in mango improvement programme.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2021-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42539120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Molecular characterization of oats (Avena sativa L.) germplasm with microsatellite markers 燕麦（Avena sativa L.）种质的微卫星标记分子鉴定

IF 1 4区生物学

Indian Journal of Genetics and Plant Breeding Pub Date : 2021-03-25 DOI: 10.31742/ijgpb.81.1.18

G. Kaur, R. Kapoor, Priti Sharma, P. Srivastava

引用次数: 0

Selection of elite parents and crosses for seed yield and its components using combining ability analysis over the three environments in safflower (Carthamus tinctorious L.) 三种环境下红花（Carthamus tinctorious L.）优良亲本和杂交种产量及其组成的配合力分析

IF 1 4区生物学

Indian Journal of Genetics and Plant Breeding Pub Date : 2021-03-25 DOI: 10.31742/ijgpb.81.1.17

Indrayani H. Thorat, V. Gawande

引用次数: 4

Cytogenetic studies of three cultivated hill cotton (Gossypium arboreum L.) varieties from Bangladesh 孟加拉国三个栽培山棉品种的细胞遗传学研究

IF 1 4区生物学

Indian Journal of Genetics and Plant Breeding Pub Date : 2021-03-25 DOI: 10.31742/ijgpb.81.1.16

A. Hossain, C. Dash, Syeda Sharmeen Sultana

引用次数: 0

Identification of pigeonpea genotypes with wider adaptability to rainfed environments through AMMI and GGE biplot analyses 通过AMMI和GGE双图分析鉴定对雨养环境适应性较强的鸽豌豆基因型

IF 1 4区生物学

Indian Journal of Genetics and Plant Breeding Pub Date : 2021-03-25 DOI: 10.31742/ijgpb.81.1.7

M. Kumar, V. Ramya, C. S. Kumar, T. Raju, N. Kumar, G. Seshu, G. Sathish, D. Bhadru, M. .. Ramana

{"title":"Identification of pigeonpea genotypes with wider adaptability to rainfed environments through AMMI and GGE biplot analyses","authors":"M. Kumar, V. Ramya, C. S. Kumar, T. Raju, N. Kumar, G. Seshu, G. Sathish, D. Bhadru, M. .. Ramana","doi":"10.31742/ijgpb.81.1.7","DOIUrl":"https://doi.org/10.31742/ijgpb.81.1.7","url":null,"abstract":"Pigeonpea [Cajanus cajan (L.) Millspaugh] is an important pulse crop grown under Indian rainfed agriculture. Twenty eight pigeonpea genotypes were tested for stability and adaptability across ten rainfed locations in the States of Telangana and Karnataka, India using AMMI (additive main effects and multiplicative interaction) model and GGE (genotype and genotype by environment) biplot method. The grain yields were significantly affected by environment (56.8%) followed by genotype × environment interaction (27.6%) and genotype (18.6%) variances. Two mega environments were identified with several winning genotypes viz., ICPH 2740 (G15), TS 3R (G10), PRG 176 (G8) and ICPL 96058 (G22). E2 (Gulbarga, Karnataka), E3 (Bidar, Karnataka) and E6 (Vikarabad, Telangana) were the most discriminating environments. Genotypes, ICPH 2740, PRG 176 and TS 3R were the best cultivars in all the environments whereas PRG 158 (G9), ICPL 87119 (G12), ICPL 20098 (G19) and ICPL 96058 (G22) were suitable across a wide range of environments. Genotypes, ICPH 2740 and PRG 176 can be recommended on a large scale to the farmers with small holdings to enhance pigeonpea productivity and improve the food security","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2021-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48985284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Pattern of genetic variation in rice (Oryza sativa L.) population for sheath blight resistance over the seasons 水稻(Oryza sativa L.)群体抗叶枯病的遗传变异模式

IF 1 4区生物学

Indian Journal of Genetics and Plant Breeding Pub Date : 2021-03-25 DOI: 10.31742/ijgpb.81.1.15

R. Naveenkumar, Vineeta Singh, P. Singh, A. Anandan

引用次数: 4

Molecular tagging of Rf genes for the fertility restoration of WA-CMS system by bulk segregant analysis in rice 水稻WA-CMS系统育性恢复中Rf基因的分子标记

IF 1 4区生物学

Indian Journal of Genetics and Plant Breeding Pub Date : 2021-03-25 DOI: 10.31742/ijgpb.81.1.4

V. Thakur, Revathi Ponnuswamy, A. Singh, V. Shankar, Srinivasa D. Chary

{"title":"Molecular tagging of Rf genes for the fertility restoration of WA-CMS system by bulk segregant analysis in rice","authors":"V. Thakur, Revathi Ponnuswamy, A. Singh, V. Shankar, Srinivasa D. Chary","doi":"10.31742/ijgpb.81.1.4","DOIUrl":"https://doi.org/10.31742/ijgpb.81.1.4","url":null,"abstract":"The process of screening for fertility restoration trait involves test crossing with a set of cytoplasmic male sterile (CMS) lines and evaluation of F1 hybrids for pollen and spikelet fertility. In the present study, F2 mapping population derived from a cross, APMS 6A × RP 5933-123 was utilized to map Rf genes. The F2 population was also genetically analysed for pollen and spikelet fertility percentage. Chisquare (?2) analysis to showed that the fertility restoration trait followed expected digenic ratio. By bulk segregant analysis (BSA) likely Rf genes containing regions were located on chromosome 10. The SSR markers viz., RM304, RM258 located on chromosome 10 and RM23958 located on chromosome 9 showed clear polymorphism between two groups of fertile and sterile bulks. Based on BSA linkage analysis and F2 population, pollen and spikelet fertility analysis along with molecular screening results of Rf linked markers, it is concluded that Rf4 gene located on chromosome 10 is playing major role and contributing to 90% of fertility restoration trait of newly derived restorer line RP5933 along with minor effect genes from chromosome 9. The findings may be useful for rice hybrid breeding.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2021-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47184711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterizing the diversity of grain phytic acid content in Indian rice landraces by multivariate genetic analyses 印度地方稻籽粒植酸含量多样性的多变量遗传分析

IF 1 4区生物学

Indian Journal of Genetics and Plant Breeding Pub Date : 2021-03-25 DOI: 10.31742/ijgpb.81.1.3

Prem Chand Gyani, H. Bollinedi, S. G. Krishnan, K. Vinod, P. Bhowmick, M. Nagarajan, N. Singh, R. Ellur, Ashok K. Singh

{"title":"Characterizing the diversity of grain phytic acid content in Indian rice landraces by multivariate genetic analyses","authors":"Prem Chand Gyani, H. Bollinedi, S. G. Krishnan, K. Vinod, P. Bhowmick, M. Nagarajan, N. Singh, R. Ellur, Ashok K. Singh","doi":"10.31742/ijgpb.81.1.3","DOIUrl":"https://doi.org/10.31742/ijgpb.81.1.3","url":null,"abstract":"Phytic acid (PA) acts as chelator of cationic mineral elements iron (Fe) and zinc (Zn) and obstructs their absorption in the human gut. We have evaluated a set of 162 traditional rice landraces for phytic acid phosphorus (PA-P), inorganic phosphorus (Pi) and total phosphorus (TP). Wide variability was observed for PA-P, Pi and TP ranging from 1.12 to 3 mg/g, 0.004 to 0.16 mg/g and 1.17 to 3.04 mg/g respectively. The mineral micronutrients, Fe and Zn were not in correlation with PA-P, Pi and TP implicating the possibility of their independent improvement while PAP showed a significant positive correlation with TP and significant negative correlation with Pi. Principal component analysis (PCA) identified two principal components PC1 and PC2, explaining 50.6 % and 32.1 % of the total variation, respectively. Cluster analysis grouped the accessions into four clusters. The study has also led to the identification of promising donors such as P1490 and Gowri with low PA content to be utilized in rice biofortification programmes.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2021-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46814272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1