Identification of potential donor for pro-vitamin A using functional markers in maize (Zea mays L)

IF 1 4区 生物学 Q3 PLANT SCIENCES
A. Das, A. Singode, D. Chaudhary, Yathish Kr, Chikkappa Gangadhar Karjagi, Ramesh Kumar, B. Kumar, Vishal Singh, G. Mukri, Sapna, S. Rakshit
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引用次数: 0

Abstract

Vitamin A deficiency (VAD) is a global health problem. Maize naturally accumulates carotenoids including ?-carotene, a major source of vitamin A. Thirty-seven Indian maize genotypes were screened for total carotenoids and ?carotene content. A positive significant association of kernel colour was observed with total carotenoid but not for ?-carotene. The level of ?-carotene among the selected 37 genotypes was low (0.14 to 4.63µg/g) as compared to total carotenoids (15.94 to 66.46 µg/g) thus necessitates the introduction and deployment of lines with high ?carotene. Hence, 54 inbred lines developed under the HarvestPlus programme were screened with functional markers of lcyE and crtRB1 genes of the carotenoid biosynthetic pathway. Three genotypes (HP704-13, HP704- 22, and HP704-23) amplified most of the favorable alleles for both these genes. However, due to long Anthesis Silking Interval (ASI) and poor adaptation HP704-13 and HP704-23 could not be maintained. Under normal storage condition genotype, HP704-22 recorded 7.32 µg/g of ?-carotene which can be used as a donor for provitamin-A or to diversify the Indian germplasm.
利用玉米(Zea mays L)功能标记鉴定维生素A原的潜在供体
维生素A缺乏症是一个全球性的健康问题。玉米天然积累的类胡萝卜素包括-胡萝卜素,这是维生素a的主要来源。37个印度玉米基因型进行了总类胡萝卜素和-胡萝卜素含量的筛选。籽粒颜色与总类胡萝卜素呈显著正相关,与-胡萝卜素无显著正相关。与总类胡萝卜素含量(15.94 ~ 66.46µg/g)相比,37个基因型的-胡萝卜素含量(0.14 ~ 4.63µg/g)较低,因此需要引进和配置高-胡萝卜素的品系。因此,在HarvestPlus计划下培育的54个自交系用类胡萝卜素生物合成途径的lcyE和crtRB1基因的功能标记进行了筛选。三个基因型(HP704-13、HP704- 22和HP704-23)扩增了这两个基因的大部分有利等位基因。但由于开花吐丝间隔长,适应性差,无法维持HP704-13和HP704-23。在正常贮藏条件下,基因型HP704-22的-胡萝卜素含量为7.32µg/g,可作为维生素a原的供体或使印度种质资源多样化。
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来源期刊
CiteScore
1.80
自引率
10.00%
发文量
0
审稿时长
6-12 weeks
期刊介绍: Advance the cause of genetics and plant breeding and to encourage and promote study and research in these disciplines in the service of agriculture; to disseminate the knowledge of genetics and plant breeding; provide facilities for association and conference among students of genetics and plant breeding and for encouragement of close relationship between them and those in the related sciences; advocate policies in the interest of the nation in the field of genetics and plant breeding, and facilitate international cooperation in the field of genetics and plant breeding.
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