In Vitro Cellular & Developmental Biology - Plant最新文献

{"title":"Enhancement in the production of phenolic compounds from Fagonia indica callus cultures via Fusarium oxysporum triggered elicitation","authors":"Taimoor Khan, Muhammad Uzair Javed, Tehreem Mahmood, Bushra Khan, Tariq Khan, Muhammad Asad Ullah, Razia Khurshid, Gouhar Zaman, Christophe Hano, Nathalie Giglioli-Guivarc’h, Bilal Haider Abbasi","doi":"10.1007/s11627-023-10358-0","DOIUrl":"https://doi.org/10.1007/s11627-023-10358-0","url":null,"abstract":"<p><i>Fagonia indica</i> Burm.f. <i>(1768)</i> is a medicinally important plant showing diverse pharmaceutical benefits. It is renowned for its ability to biosynthesize several anticancer and anti-inflammatory metabolites. For the eco-friendly and sustainable synthesis of phytochemicals and plant biomass, a biotechnological technique, “elicitation,” is a highly effective method in various <i>in vitro</i> cultures. The present study includes using various concentrations of <i>Fusarium oxysporum</i> Schlecht. as an elicitor in callus cultures of <i>Fagonia indica</i>. The main goal was to achieve enhancement in biomass production and secondary metabolism. The findings demonstrated that maximum biomass production (FW: 167.42 ± 3.99 g per 100 mL; DW: 12.53 ± 1.04 g per 100 mL) was observed at 50 mg L⁻¹ of <i>Fusarium oxysporum</i> as compared to the control. Secondary metabolites showed immense production (phenolic content (9.68 ± 0.23 µg mg⁻¹); flavonoid content (2.814808 ± 0.11 µg mg⁻¹)) in callus cultures treated with 10 mg L⁻¹ of <i>Fusarium oxysporum</i> as compared with control. Moreover, the cultures possessed the highest antioxidant capacity, as determined by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS^•+) radical cation based assay and α, α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging assay, ((821.51 ± 3.20 µmol TEAC per mg DW of ABTS inhibition) (91% ± 1.45 of DPPH inhibition)) at 10 mg L⁻¹ concentration of <i>Fusarium oxysporum</i>, and the maximum ferric ion reducing activity (219.29 ± 2.36 µmol TEAC per mg DW) was noticed at 1.0 mg L⁻¹ concentration of <i>F. oxysporum</i>. <i>Fagonia indica</i> cultures also indicated the highest percent inhibition against cyclooxygenases (COX-1: 51.93% ± 1.74 and COX-2: 40.57% ± 1.99), lipoxygenase (15-LOX: 65.72% ± 1.44), and phospholipase A2 (sPLA2: 49.29% ± 1.75), when treated with different concentrations of <i>F. oxysporum</i>. HPLC analyses showed a significant accumulation of pharmacologically active components in the treated samples, with kaempferol (1245.56 mg g⁻¹) and myricetin (1139.63 mg g⁻¹) as the most accumulated compounds in the cultures with 10.0 mg L⁻¹ concentration of <i>Fusarium</i> in contrast to the control. These findings revealed that in callus cultures of <i>F. indica</i>, <i>F. oxysporum</i> could boost biomass accumulation and secondary metabolite production.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"3 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139465436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Androgenesis in soybean (Glycine max (L.) Merr.): a critical revisit","authors":"Ayyagari Ramlal, Sahil Mehta, Aparna Nautiyal, Pooja Baweja, Shivam, Deepshikha Sharma, S. K. Lal, Roshni Vijayan, Dhandapani Raju, Sreeramanan Subramaniam, Ambika Rajendran","doi":"10.1007/s11627-023-10402-z","DOIUrl":"https://doi.org/10.1007/s11627-023-10402-z","url":null,"abstract":"<p><i>Glycine max</i> (L.) Merr. (soybean) is a multi-purpose crop used for both animal and human feed. It is an economically and industrially important crop. It possesses many therapeutical and nutraceutical compounds. Therefore, soybean is referred to as ‘Gold from the soil’. Conventional breeding approaches are laborious and time-consuming. Thus, alternative biotechnological methods, such as <i>in vitro</i> micropropagation, regeneration, and transformation, could be advantageous. Despite the efforts made in the field of soybean micropropagation for haploidy and doubled haploidy, especially androgenesis, the success rate accounts for only approximately 2%. Androgenesis in soybean is lacking primarily due to its recalcitrant nature and differences in the development of microspores within a flower. Haploids and doubled haploids (DHs) have contributed immensely to crop improvement programs. In this review, soybean androgenesis history, production of haploids, and doubled haploids have been highlighted. The factors responsible for the androgenic responses have also been discussed. Furthermore, the review will be helpful in understanding the challenges in the standardization of protocol for the production of haploids and DHs in soybean, which will eventually assist breeding and crop improvement.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"24 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139105216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alpha-amylase inhibitory activity and in silico studies of in vitro sweet basil plantlets treated with chitosan and ZnO NPs","authors":"","doi":"10.1007/s11627-023-10401-0","DOIUrl":"https://doi.org/10.1007/s11627-023-10401-0","url":null,"abstract":"<h3>Abstract</h3> <p>In this study, an efficient protocol was developed to obtain multiplicated, healthy sweet basil plantlets with larger leaves and a greener color by culturing them on MS medium containing 1.0 mg/l 2iP. Chitosan and zinc oxide nanoparticles (ZnO NPs) were used as abiotic elicitors to maximize the main secondary metabolites (phenolic and flavonoid compounds and essential oil) in <em>in vitro</em> plantlets of sweet basil. Chitosan with a concentration of 4.0 mg/l recorded the maximum value of caffeic acid (1.189 mg/g DW), and 10 mg/l of ZnO NPs recorded the maximum content of rutin (0.936 mg/g DW). The plantlets cultured with 2.0 mg/l chitosan recorded the maximum percentage of essential oil (90.63%). 8,9-Dihydro-9-formyl cycloisolongifolene was the major compound (72.44%) and was detected in the plantlets treated with 2.0 mg/l chitosan. The inhibitory activity evaluation of α-amylase revealed that the maximum inhibitory activity was found in the plantlet control and those treated with 4.0 mg/l chitosan of the phenolic and flavonoid fractions. The maximum IC₅₀ (7.36 ± 0.85) was found in the plantlet extract treated with 4.0 mg/l chitosan, followed by the plantlet control, which recorded 11.53 ± 0.86. Using AutoDuck 4.2 and PyMol software, it was revealed that rutin and caffeic acid compounds (the main active components) own hydrogen binding interactions and hydrophobic binding interactions with the active site of alpha-amylase from the porcine pancreas (PPA) enzyme with good binding energy (5.74 and 5.71 kcal/mol, respectively).</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"13 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139082701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bergenia ligulata (Wall.): micropropagation, genetic fidelity, and SEM studies","authors":"","doi":"10.1007/s11627-023-10398-6","DOIUrl":"https://doi.org/10.1007/s11627-023-10398-6","url":null,"abstract":"<h3>Abstract</h3> <p><em>Bergenia</em> <em>ligulata</em>, commonly known as ‘Pashanbheda’ or Indian rhubarb, is a perennial herb that has been recognized for its diverse medicinal properties. The indiscriminate use of <em>B. ligulata</em> has brought the species to the brink of becoming threatened. This research aims to establish a robust tissue culture protocol that can be utilized for the rapid micropropagation of <em>B. ligulata</em>. This protocol is essential for ensuring the sustainable production of this valuable plant species and preventing the depletion of its natural populations. The study successfully demonstrated an efficient <em>in vitro</em> regeneration in <em>B. ligulata</em>, using leaf and petiole explants. The most effective combination for achieving the highest number of shoots on either explant (leaf or petiole) involved using Murashige and Skoog (MS) medium supplemented with 0.9 µM and 1.8 µM 6-benzylamino purine (BAP) with 0.5 µM 1-naphthaleneacetic acid (NAA). Moreover, multiple shoots were also produced on MS medium fortified with 8.8 µM BAP and 2.3 µM kinetin (Kn). To achieve optimal rooting, the 45-d-old shoot was carefully isolated and placed in a half-strength MS medium. PCR-based molecular analysis using inter simple sequence repeats (ISSR) confirmed the genetically clonal nature of regenerated plantlets. About 80% of the well-developed <em>in vitro</em> regenerated plants were acclimatized in the glasshouse, thereby showing the robustness of the developed protocol. Based on the present study, a reproducible <em>in vitro</em> technique was utilized to achieve direct regeneration of approximately 3597 plants from a single explant over a 1-yr period. This approach involved molecular fidelity analysis and scanning electron microscopy (SEM) analyses to ensure reliable results.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"17 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139071307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synseed germination, conversion, and acclimatization of seedless watermelon ‘Quality’","authors":"Putri Santika, Jong-Yi Fang","doi":"10.1007/s11627-023-10403-y","DOIUrl":"https://doi.org/10.1007/s11627-023-10403-y","url":null,"abstract":"<p>Seedless watermelons are triploid hybrids (3n) that have been growing in popularity since the 1990s. However, the high production cost and low germination rate of the hybrid seeds pose a major problem in their production. The synseed technology could make a promising alternative for seedless watermelon propagation; however, this alternative has not yet been explored. This study aimed at producing synseeds of seedless watermelon ‘Quality’ using shoot tips as the propagules after which their germination and conversion were evaluated under <i>in vitro</i> and <i>ex vitro</i> conditions. <i>In vitro</i> shoot multiplication with different concentrations of 6-benzylaminopurine (BAP), synseed production from BAP-multiplied shoot tips, synseed sowing <i>in vitro</i> using several substrates and medium supplements, and synseed sowing <i>ex vitro</i> using different antimicrobial agents were investigated. Results indicated that 1.0 mg L⁻¹ BAP resulted in the highest number of shoots per shoot tip explant. Synseeds produced from BAP-multiplied shoot tips achieved faster germination compared to synseeds from non-BAP-derived shoot tips, but there was no difference in terms of conversion and acclimatization survival rates. Agar showed greater conversion and acclimatization survival rates as well as root number, root length, and shoot length than the other substrates especially when indole-3-butyric acid (IBA) at 1.0 mg L⁻¹ was added to the medium. However, this study demonstrated the possibility of using vermiculite as an alternative substrate to agar. PPM at 0.5% was a better microbial agent than 100.0 mg L⁻¹ mancozeb for <i>ex vitro</i> synseed sowing as it achieved zero contamination and maintained the growing potential of synseeds with 65% germination, 85% survival, and 25% rooting rates.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"123 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139071310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the impact of sodium nitroprusside on morphogenesis, selected phytochemical profiling, and antioxidant activities of in vitro–raised plantlets of Citrus indica Yu. Tanaka","authors":"M. Wanlambok Sanglyne, Meera Chettri Das","doi":"10.1007/s11627-023-10400-1","DOIUrl":"https://doi.org/10.1007/s11627-023-10400-1","url":null,"abstract":"<p>The objective of this study was to assess the effect of sodium nitroprusside (SNP) on the morphology, physiology, and biochemistry of <i>Citrus indica</i> Yu. Tanaka, which is considered a native and endangered species in northeast India. Moreover, <i>C. indica</i> seeds do not germinate well in their natural habitat, and the species does not have a good commercial standing in terms of valuable metabolite synthesis. Therefore, an efficient quick-growth method with improved phytochemical synthesis has been accomplished. For mass production employing micropropagation with embryos as explants, Murashige and Skoog (MS) medium was supplemented with 0.2 µM, 0.4 µM, and 0.6 µM of SNP, 3.0 µM of 6-benzylaminopurines (BAP), and 4.0 µM of thidiazuron (TDZ). The results showed that 0.4 µM of SNP alone or in conjunction with 3.0 µM of BAP was sufficient to improve morphological characteristics. In addition, established clones were examined for membrane stability by measuring electrolyte leakage (EL) and malondialdehyde (MDA), as well as photosynthetic pigment and carotenoids. In almost all the cases, high values were recorded when 0.4 µM of SNP was used, either singly or in conjunction with 3.0 µM of BAP in the MS medium. Clonally produced plantlets were also subjected to phytochemical screening and antioxidant activities, such as hydrogen peroxide (H₂O₂), superoxide anion, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, wherein these tests also revealed incorporation of SNP exhibited enhanced phytochemical concentrations and antioxidant activities. This research can thus be used to mass produce <i>in vitro</i> plantlets with considerably higher quantities of secondary metabolites, which may make them more resistant to environmental challenges than their natural counterparts without treatment.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"15 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139051898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized protocol for isolation and transient expression of placenta-originated protoplast in pepper (Capsicum annuum L.)","authors":"","doi":"10.1007/s11627-023-10404-x","DOIUrl":"https://doi.org/10.1007/s11627-023-10404-x","url":null,"abstract":"<h3>Abstract</h3> <p>Pepper (<em>Capsicum annuum</em> L.) is one of the most widely cultivated species and is highly valued for its pungency. The pungency of pepper is primarily attributed to a group of chemical compounds known as capsaicinoids. These compounds are synthesized in the placental tissue of pepper fruits through the activation of specific genes and enzymes, thereby contributing to their pungency. Protoplast-based gene expression systems have been considered an efficient method for gene function studies, protein-protein interactions, promoter analysis, and subcellular localization. Here, we optimized an efficient protocol for isolating protoplasts from pepper placental tissues and the polyethylene glycol (PEG)-mediated transient expression of green fluorescent proteins (GFP). Several factors affecting GFP expression in intact protoplasts were evaluated and optimized in this study. Protoplast isolation was carried out using 2.0% cellulase “onozuka” R-10 and 0.3% macerozyme R-10 solution. Different amounts of plasmid DNA and various incubation times for transfection with 40% PEG resulted in different transfection efficiencies (78–85%). The highest GFP transformation efficiency was observed when 120 µL protoplast suspension (3 × 10⁷ to 5 × 10⁷/mL) was mixed with 15 µg plasmid DNA and incubated for 25 min with an equal volume of 40% PEG. The improved protocol described in this study can be helpful for the isolation and transfection of pepper placenta-originated protoplasts and for the rapid investigation of pepper gene functions.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"32 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139031212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organogenesis versus somatic embryogenesis pathway efficiencies in in vitro propagation of white and water yams","authors":"Chukwunalu O. Ossai, Morufat O. Balogun, Norbert G. Maroya","doi":"10.1007/s11627-023-10397-7","DOIUrl":"https://doi.org/10.1007/s11627-023-10397-7","url":null,"abstract":"<p>The primary goal of this study was to compare the multiplication rates of yam varieties propagated through organogenesis and somatic embryogenesis (SE). Callus was induced from axillary bud explants of three genotypes of <i>Dioscorea rotundata</i> (Asiedu, Ekiti2a, and Kpamyo) and two genotypes of <i>Dioscorea alata</i> (Swaswa and TDa2014) cultured in Murashige and Skoog (MS) medium containing 9.1 µM 2,4-dichlorophenoxylacetic acid and 5.4 µM naphthaleneacetic acid. Plantlets were regenerated in MS containing 4.4 µM benzylaminopurine and 34 µM uniconazole-P through SE. Single-node cuttings of the five genotypes were grown in MS for 8 wk <i>via</i> organogenesis. The SE and organogenesis regenerants were acclimatized and potted in a 2 (propagation techniques (PTs)) × 5 (genotypes) factorial arranged in a completely randomized design (<i>r</i> = 10). The multiplication ratios (MR), number of tubers (NoT) of the SE, and organogenesis regenerants were collected and analyzed using ANOVA, and means were separated using DMRT (<i>P</i> ≤ 0.05). The SE and organogenesis MR ranged from 1:2 (TDa2014) to 1:8 (Asiedu) and 1:4 (Asiedu) to 1:5 (Ekiti2a and TDa2014), respectively. The NoT differed among genotypes, ranging from 1.15 ± 0.49 (Swaswa) to 2.45 ± 1.39 (Asiedu), and between PTs, ranging from 1.42 ± 0.70 (SE) to 1.86 ± 1.11 (organogenesis). The optimum propagation pathway was genotype-specific.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"22 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138518012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Micropropagation of Lepidium ostleri (Brassicaceae), a native endemic plant species","authors":"Alyson M. DeNittis, Joseph M. Larson, Olga Ruiz Kopp","doi":"10.1007/s11627-023-10376-y","DOIUrl":"https://doi.org/10.1007/s11627-023-10376-y","url":null,"abstract":"<p><i>Lepidium ostleri</i> S.L. Welsh &amp; Goodrich (Ostler’s peppergrass) is an endemic plant species restricted to Ordovician limestone outcrops associated with the San Francisco Mountain Range in western Utah. Due to restricted population distribution and proximity to modern mining operations, <i>L. ostleri</i> is a species of conservation interest. This study focused on the development of a micropropagation protocol for propagating mature plants using plant tissue culture methods. Indirect shoot organogenesis was obtained from <i>L. ostleri</i> explants on Murashige and Skoog (MS) medium augmented with various concentrations of BAP (6-Benzylaminopurine), kinetin (N6-furfuryladenine), and IAA (indole-3-acetic acid). Plantlets supporting shoots grown <i>in vitro</i> were pulse treated with differing strengths of indole-3-butyric acid (IBA) and transferred to sterile soil. Following root induction, plantlets were acclimated to ambient conditions. The successful development of a micropropagation protocol supports management activities for <i>L. ostleri</i> and also contributes to <i>in vitro</i> propagation knowledge at the species, genus, and family levels.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"43 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138517947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mineral analysis of Corymbia maculata plantlets: a shortcut to optimize the mineral concentrations in tissue culture media","authors":"Jonathan Lidbetter, Antoine Martin, Sophie Parks, Paul Holford, P. Milham","doi":"10.1007/s11627-023-10372-2","DOIUrl":"https://doi.org/10.1007/s11627-023-10372-2","url":null,"abstract":"","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"21 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74119768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}