Immunobiology最新文献

{"title":"The changes of intestinal flora and its relevance with memory Tfh and B cells in rheumatoid arthritis patients treated with csDMARDs and csDMARDs + bDMARDs","authors":"Chunlei Jiang ,&nbsp;Shuhong Chi ,&nbsp;Fengkui Wang ,&nbsp;Chenyang Zhao ,&nbsp;Xiaojuan Yang ,&nbsp;Miao Liu ,&nbsp;Bin Ma ,&nbsp;Jian Chen ,&nbsp;Chunxia Su ,&nbsp;Xiangguo Duan","doi":"10.1016/j.imbio.2024.152798","DOIUrl":"10.1016/j.imbio.2024.152798","url":null,"abstract":"<div><h3>Background</h3><p>A growing body of experimental and clinical evidence has implicated gut microbiota in the onset and course of rheumatoid arthritis (RA). The imbalance of intestinal flora in RA patients may lead to abnormal expression of immune cells and related cytokines.</p></div><div><h3>Purpose</h3><p>Conventional synthetic disease-modifying antirheumatic drugs (csDMARDs) and conventional synthetic disease-modifying antirheumatic drugs combined with biological disease-modifying antirheumatic drugs (csDMARDs + bDMARDs) are widely used to treat RA, but the characteristics of gut microbiota before and after treatment and their relationship with memory Tfh/B cells and cytokines remain unclear.</p></div><div><h3>Methods</h3><p>Stool samples were collected from 50 RA patients and 25 healthy controls (HCs) for 16SrRNA gene sequencing. We examined the proportion of lymphocyte subsets in healthy controls and RA patients. Enzyme linked immunosorbent assay (ELISA) was used to detect the levels of related cytokines in serum. The α and β diversity of intestinal flora, and the correlation between intestinal flora and clinical indicators, lymphocyte subsets, cytokines were analyzed.</p></div><div><h3>Result</h3><p>At the genus level, Ruminococcaceae_Ruminococcus was decreased in the csDMARDs and csDMARDs + bDMARDs treatment group, whereas Faecalibacterium was reduced in the csDMARDs treatment group, compared to untreated group. CD4⁺CD45RO⁺CCR7⁺CXCR5⁺central memory Tfh cells and CD4⁺CD45RO⁺CCR7^-CXCR5⁺effector memory Tfh cells were significantly lower in the csDMARDs + bDMARDs treatment group than in untreated group. CD19⁺CD27⁺IgD⁺pre-switched memory B cells were higher in the csDMARDs and csDMARDs + bDMARDs treatment groups, whereas CD19⁺CD27⁺IgD^-switched memory B cells were significantly lower than in untreated group. Ruminococcaceae_Ruminococcus was negatively correlated with CD19⁺CD27⁺IgD⁺ pre-switched memory B cells but positively correlated with CD4⁺CD45RO⁺CCR7^-CXCR5⁺effector memory Tfh and CD19⁺CD27⁺IgD^-switched memory B cells in patients with RA treated with DMARDs.</p></div><div><h3>Conclusion</h3><p>The gut microbiota, memory Tfh cells, memory B cells, and cytokines of patients with RA changed significantly under different treatment regimens and had certain correlations with the clinical indicators of RA.</p></div>","PeriodicalId":13270,"journal":{"name":"Immunobiology","volume":"229 3","pages":"Article 152798"},"PeriodicalIF":2.8,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0171298524000160/pdfft?md5=830235ff7c7dafc5ad593cdb26294649&pid=1-s2.0-S0171298524000160-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140282134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of dapagliflozin on collectins and complement activation in plasma from patients with type 2 diabetes and albuminuria: Data from the DapKid cohort","authors":"Mia Jensen ,&nbsp;Mie K. Eickhoff ,&nbsp;Frederik Persson ,&nbsp;Peter Rossing ,&nbsp;Steffen Thiel ,&nbsp;Søren W.K. Hansen ,&nbsp;Yaseelan Palarasah ,&nbsp;Per Svenningsen ,&nbsp;Boye L. Jensen","doi":"10.1016/j.imbio.2024.152797","DOIUrl":"10.1016/j.imbio.2024.152797","url":null,"abstract":"<div><h3>Background</h3><p>Sodium-glucose cotransporter 2 (SGLT- 2) inhibitors exert cardiovascular and kidney-protective effects in people with diabetes. Attenuation of inflammation could be important for systemic protection. The lectin pathway of complement system activation is linked to diabetic nephropathy. We hypothesized that SGLT-2 inhibitors lower the circulating level of pattern-recognition molecules of the lectin cascade and attenuate systemic complement activation.</p></div><div><h3>Methods</h3><p>Analysis of paired plasma samples from the DapKid crossover intervention study where patients with type 2 diabetes mellitus (T2DM) and albuminuria were treated with dapagliflozin and placebo for 12 weeks (10 mg/day, n=36). ELISA was used to determine concentrations of collectin kidney 1 (CL-K1), collectin liver 1 (CL-L1), mannose-binding lectin (MBL), MBL-associated serine protease 2 (MASP-2), the anaphylatoxin complement factor 3a (C3a), the stable C3 split product C3dg and the membrane attack complex (sC5b-9).</p></div><div><h3>Results</h3><p>As published before, dapagliflozin treatment lowered Hba_1C from 74 (14.9) mmol/mol to 66 (13.9) mmol/mol (p&lt;0.0001), and the urine albumin/creatinine ratio from 167.8 mg/g to 122.5 mg/g (p&lt;0.0001). Plasma concentrations of CL-K1, CL-L1, MBL, and MASP-2 did not change significantly after dapagliflozin treatment (P&gt;0.05) compared to placebo treatment. The plasma levels of C3a (P&lt;0.05) and C3dg (P&lt;0.01) increased slightly but significantly, 0.6 [0.2] units/mL and 76 [52] units/mL respectively, after dapagliflozin treatment. The C9-associated neoepitope in C5b-9 did not change in plasma concentration by dapagliflozin (P&gt;0.05).</p></div><div><h3>Conclusion</h3><p>In patients with type 2 diabetes and albuminuria, SGLT-2 inhibition resulted in modest C3 activation in plasma, likely not driven by primary changes in circulating collectins and not resulting in changes in membrane attack complex. Based on systemic analyses, organ-specific local protective effects of gliflozins against complement activation cannot be excluded.</p></div>","PeriodicalId":13270,"journal":{"name":"Immunobiology","volume":"229 3","pages":"Article 152797"},"PeriodicalIF":2.8,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0171298524000159/pdfft?md5=c412cbc6629b0d43b57c13f30c145a8a&pid=1-s2.0-S0171298524000159-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140181910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcription suppression of GABARAP mediated by lncRNA XIST-EZH2 interaction triggers caspase-11-dependent inflammatory injury in ulcerative colitis","authors":"Dan Gu ,&nbsp;Ting Cao ,&nbsp;Shijie Yi ,&nbsp;Xiaoqian Li ,&nbsp;Ya Liu","doi":"10.1016/j.imbio.2024.152796","DOIUrl":"10.1016/j.imbio.2024.152796","url":null,"abstract":"<div><h3>Background</h3><p>We have previously found that enhancer of zeste homolog 2 (EZH2) is correlated with inflammatory infiltration and mucosal cell injury in ulcerative colitis (UC). This study aims to analyze the role of X-inactive specific transcript (XIST), a possible interactive long non-coding RNA of EZH2, in UC and to explore the mechanisms.</p></div><div><h3>Methods</h3><p>C57BL/6N mice were treated with dextran sulfate sodium (DSS), and mouse colonic mucosal epithelial cells were treated with DSS and lipopolysaccharide (LPS) for UC modeling. The UC-related symptoms in mice, and the viability and apoptosis of mucosal epithelial cells were determined. Inflammatory injury in animal and cellular models were assessed through the levels of ACS, occludin, IL-1β, IL-18, TNF-α, caspase-1, and caspase-11. Molecular interactions between XIST, EZH2, and GABA type A receptor-associated protein (GABARAP) were verified by immunoprecipitation assays, and their functions in inflammatory injury were determined by gain- or loss-of-function assays.</p></div><div><h3>Results</h3><p>XIST was highly expressed in DSS-treated mice and in DSS + LPS-treated mucosal epithelial cells. It recruited EZH2, which mediated gene silencing of GABARAP through H3K27me3 modification. Silencing of XIST alleviated body weight loss, colon shortening, and disease active index of mice and reduced inflammatory injuries in their colon tissues. Meanwhile, it reduced apoptosis and inflammation in mucosal epithelial cells. However, these alleviating effects were blocked by either EZH2 overexpression or GABARAP knockdown. Rescue experiments identified caspase-11 as a key effector mediating the inflammatory injury following GABARAP loss.</p></div><div><h3>Conclusion</h3><p>This study suggests that the XIST-EZH2 interaction-mediated GABARAP inhibition activates caspase-11-dependent inflammatory injury in UC.</p></div>","PeriodicalId":13270,"journal":{"name":"Immunobiology","volume":"229 3","pages":"Article 152796"},"PeriodicalIF":2.8,"publicationDate":"2024-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0171298524000147/pdfft?md5=b60fa3a4a6fe804afbff7824d492d409&pid=1-s2.0-S0171298524000147-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140054399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The predictive value of miR-377 and phospholipase A2 in the early diagnosis of diabetic kidney disease and their relationship with inflammatory factors","authors":"Chenhao Xing ,&nbsp;Lijing Huo ,&nbsp;Hongyue Tang ,&nbsp;Yamin Lu ,&nbsp;Guangxia Liu ,&nbsp;Fang Chen ,&nbsp;Zhan Hou","doi":"10.1016/j.imbio.2024.152792","DOIUrl":"10.1016/j.imbio.2024.152792","url":null,"abstract":"<div><h3>Objective</h3><p>The value of novel biomarkers for DKD has received increasing attention, and there is an urgent need for novel biomarkers with sensitivity, specificity and ability to detect kidney damage.miR-377 regulates many basic biological processes, plays a key role in tumor cell proliferation, migration and inflammation, and can also increase the expression of matrix proteins and fibronectin, leading to renal tubulointerstitial inflammation and renal fibrosis. Lipoprotein-associated phospholipase A2, as an inflammatory marker, is involved in the pathological process of microalbuminuria production and renal function decline, and is a predictive factor of microalbuminuria production and renal function decline, and can be used as an indicator to evaluate the progression of DKD.The aim of this study was to investigate the effects of miR-377 and phospholipase A2 on the development of diabetic kidney disease through regulation of inflammatory factors and the mechanism of action.<strong>Methods:</strong> 80 diabetic patients were divided into two groups according to urinary albumin-to-creatinine ratio (UACR): diabetic normal proteinuria group (n = 42) and diabetic proteinuria group (n = 38). Forty-three healthy people were selected as the normal control group. The serum levels of TGF-β, IL-6, and IL-18 were measured by ELISA, miR-377 was detected by qPCR, and the serum levels of phospholipase A2 were detected by electrochemiluminescence. Analyze the correlation of study group indicators, ROC curve was used to evaluate the diagnostic efficacy of miR-377 and phospholipase A2 in diabetic kidney disease. <strong>Results:</strong> The average levels of serum TGF-β, IL-6, IL-18, miR-377 and phospholipase A2 in diabetic proteinuria group were significantly higher than those in normal control group and diabetic proteinuria normal group(P &lt; 0.05). miR-377, phospholipase A2 were significantly correlated with inflammatory factors such as glomerular filtration rate and TGF-β. miR-377 and phospholipase A2 are independent predictors of diabetic kidney disease. The area under the curve of miR-377 and phospholipase A2 in the normal diabetic proteinuria group and the diabetic proteinuria group were 0.731 and 0.744, respectively. <strong>Conclusion:</strong> miR-377 and phospholipase A2 have good diagnostic efficiency for the early diagnosis of diabetic kidney disease. They can be used as early biomarkers.miR-377 and phospholipase A2 were positively correlated with inflammatory factors and involved in the occurrence and development of diabetic kidney disease.</p></div>","PeriodicalId":13270,"journal":{"name":"Immunobiology","volume":"229 2","pages":"Article 152792"},"PeriodicalIF":2.8,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S017129852400010X/pdfft?md5=6983194224f2bce5aa03350613e28105&pid=1-s2.0-S017129852400010X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139879127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and validation of potential biomarkers related to oxidative stress in idiopathic pulmonary fibrosis","authors":"","doi":"10.1016/j.imbio.2024.152791","DOIUrl":"10.1016/j.imbio.2024.152791","url":null,"abstract":"<div><p>Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, fibrotic interstitial pneumonia with a poor prognosis and a pathogenesis that has not been fully elucidated. Oxidative stress is closely associated with IPF. In this research, we aimed to identify reliable diagnostic biomarkers associated with the oxidative stress through bioinformatics techniques. The gene expression profile data from the GSE70866 dataset was retrieved from the gene expression omnibus (GEO) database. We extracted 437 oxidative stress-related genes (ORGs) from gene set enrichment analysis (GSEA). The GSE141939 dataset was used for single-cell RNA-seq analysis to identify the expression of diagnostic genes in different cell clusters. A total of 10 differentially expressed oxidative stress-related genes (DE-ORGs) were screened. Subsequently, SOD3, CD36, ACOX2, RBM11, CYP1B1, SNCA, and MPO from the 10 DE-ORGs were identified as diagnostic genes based on random forest algorithm with randomized least absolute shrinkage and selection operator (LASSO) regression. A nomogram was constructed to evaluate the risk of disease. The decision curve analysis (DCA) and clinical impact curves indicated that the nomogram based on these seven biomarkers had extraordinary predictive power. Immune cell infiltration analysis results revealed that DE-ORGs were closely related to various immune cells, especially CYP1B1 was in positive correlation with monocytes and negative correlation with macrophages M1. Single-cell RNA-seq analysis showed that CYP1B1 was mainly associated with macrophages, and SNCA was mainly associated with basal cells. CYP1B1 and SNCA were diagnostic genes associated with oxidative stress in IPF.</p></div>","PeriodicalId":13270,"journal":{"name":"Immunobiology","volume":"229 5","pages":"Article 152791"},"PeriodicalIF":2.5,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0171298524000093/pdfft?md5=31602a8da5c42ec542df4cda524036fa&pid=1-s2.0-S0171298524000093-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139879552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transmission patterns of C1-INH deficiency hereditary angioedema favors a wild-type male offspring: Our experience at Chandigarh, India","authors":"Sanghamitra Machhua ,&nbsp;Ankur Kumar Jindal ,&nbsp;Suprit Basu ,&nbsp;Isheeta Jangra ,&nbsp;Prabal Barman ,&nbsp;Rahul Tyagi ,&nbsp;Archan Sil ,&nbsp;Reva Tyagi ,&nbsp;Anit Kaur ,&nbsp;Sanchi Chawla ,&nbsp;Sendhil M. Kumaran ,&nbsp;Sunil Dogra ,&nbsp;Manpreet Dhaliwal ,&nbsp;Saniya Sharma ,&nbsp;Amit Rawat ,&nbsp;Surjit Singh","doi":"10.1016/j.imbio.2024.152790","DOIUrl":"https://doi.org/10.1016/j.imbio.2024.152790","url":null,"abstract":"<div><h3>Background</h3><p>Deficiency of C1-inhibitor (C1-INH) protein, caused by pathogenic variants in the Serpin family G member 1 (<em>SERPING1</em>) gene, is the commonest pathophysiological abnormality (in ∼95 % cases) in patients with hereditary angioedema (HAE). C1-INH protein provides negative control over kallikrein–kinin system (KKS). Although the inheritance of the HAE-C1-INH is autosomal dominant, female predominance has often been observed in patients with HAE.</p></div><div><h3>Objective</h3><p>To analyze the risk of transmission of <em>SERPING1</em> gene variant from father or mother to their offspring.</p></div><div><h3>Methods</h3><p>Pedigree charts of 42 families with a confirmed diagnosis of HAE-C1-INH and a pathogenic variant in the <em>SERPING1</em> gene were analysed. Patients with HAE who had had at least one child were included for analyses to assess the risk of transmission from the father or mother to their offspring.</p></div><div><h3>Results</h3><p>Overall, 49 % (189/385) of all offspring inherited the genetic defect. In the subgroup analyses, 54.8 % (90/164) female offspring and 44.8 % (99/221; p &lt; 0.02) male offspring inherited the genetic defect. Inheritance of the genetic defect was significantly lower in male offspring. Fathers with <em>SERPING1</em> gene variant had a statistically significant skewed transmission of the wild type to the male offspring as compared to the variant (57.8 % wild type vs. 42.1 % variant; p &lt; 0.02), whereas no statistically significant difference was found when a father transmitted the variant to a female offspring. Mothers with <em>SERPING1</em> gene variant had no statistically significant difference in variant transmission to male or female offsprings.</p></div><div><h3>Conclusion</h3><p>Results of the study suggest that the transmission pattern of <em>SERPING1</em> gene variant favours the transmission of wild-type alleles in males, especially when the father is the carrier; hence, overall, fewer males and more female offspring inherited the variant. This could be because of a selection of wild-type male sperms during spermatogenesis, as the KLK system has been reported to play a crucial role in the regulation of spermatogenesis. Although, a similar pattern was observed in the maternal transmission of the <em>SERPING1</em> gene variant; the difference was not statistically significant, likely because of a small sample size.</p></div>","PeriodicalId":13270,"journal":{"name":"Immunobiology","volume":"229 2","pages":"Article 152790"},"PeriodicalIF":2.8,"publicationDate":"2024-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0171298524000081/pdfft?md5=254aa6bda197b894d8235c2c5f28d66d&pid=1-s2.0-S0171298524000081-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139714417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The phenotype and related gene expressions of macrophages in adipose tissue of T2D mice following MSCs infusion","authors":"Wanlu Su ,&nbsp;Yaqi Yin ,&nbsp;Yu Cheng ,&nbsp;Songyan Yu ,&nbsp;Ruofan Hu ,&nbsp;Haixia Zhang ,&nbsp;Jia Hu ,&nbsp;Rui Ren ,&nbsp;Yue Zhang ,&nbsp;Jian Zhao ,&nbsp;Anning Wang ,&nbsp;Zhaohui Lyu ,&nbsp;Yiming Mu ,&nbsp;Jieqing Gao","doi":"10.1016/j.imbio.2024.152788","DOIUrl":"10.1016/j.imbio.2024.152788","url":null,"abstract":"<div><h3>Background</h3><p>Infusion of mesenchymal stem cells (MSCs) induces polarization of M2 macrophages in adipose tissue of type 2 diabetes (T2D) mice. Studies have shown that M2 macrophages were divided into four sub-phenotypes (M2a, M2b, M2c and M2d) with different functions, and manuscripts have also confirmed that macrophages co-cultured with MSCs were not matched with known four phenotype macrophages. Therefore, our study explored the phenotype and related gene expressions of macrophages in the adipose tissue of T2D mice with/without MSCs infusion.</p></div><div><h3>Methods</h3><p>We induced a T2D mouse model by using high-fat diets and streptozotocin (STZ) injection. The mice were divided into three groups: the control group, the T2D group, and the MSCs group. MSCs were systemically injected once a week for 6 weeks. The phenotype of macrophages in adipose tissue was detected via flow cytometric analysis. We also investigated the gene expression of macrophages in different groups via SMART-RNA-sequencing and quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR).</p></div><div><h3>Results</h3><p>The present study found that the macrophages of adipose tissue in the MSCs group were polarized to the M2 phenotype mixed with four sub-phenotypes. Besides, M2a and M2c held a dominant position, while M2b and M2d (tumor-associated macrophages, TAMs) exhibited a decreasing trend after infusion of MSCs. Moreover, the MSCs group did not appear to express higher levels of tumor-associated, inflammation-associated, or fibrosis-associated genes in comparison to the T2D group.</p></div><div><h3>Conclusion</h3><p>The present results unveiled that the macrophage phenotype was inclined to be present in a hybridity state of four M2 sub-phenotypes and the genes related to tumor-promoting, pro-inflammation and pro-fibrosis were not increased after MSCs injection.</p></div>","PeriodicalId":13270,"journal":{"name":"Immunobiology","volume":"229 2","pages":"Article 152788"},"PeriodicalIF":2.8,"publicationDate":"2024-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0171298524000068/pdfft?md5=e8e42f730f82ec16086a6241e9f37293&pid=1-s2.0-S0171298524000068-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139579509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the serum levels of CCL2, CCL3, and IL-29 after first and second administrations of the COVID-19 vaccine (Oxford–AstraZeneca)","authors":"Zahra Bagheri-Hosseinabadi ,&nbsp;Ayat Kaeidi ,&nbsp;Mahdi Rezvani ,&nbsp;Gholamhossein Taghipour Khaje Sharifi ,&nbsp;Mitra Abbasifard","doi":"10.1016/j.imbio.2024.152789","DOIUrl":"10.1016/j.imbio.2024.152789","url":null,"abstract":"<div><h3>Background</h3><p>Previous studies show that chemokines and cytokines play a very important role in eliciting an appropriate response against viruses. Vaccination causes inflammation in the person receiving the vaccine, accompanied with production of inflammatory molecules by immune cells. The more and better the production and expression of chemokines and cytokines by immune cells, the better the response of the acquired immune system. Chemokines and cytokines are critical in promoting the innate immune response against the COVID-19. Here we intended to assess serum levels of CCL2, CCL3, and interleukin (IL)-29 in patients received COVID-19 vaccine.</p></div><div><h3>Methods</h3><p>In this study, 40 subjects vaccinated with the Oxford–AstraZeneca COVID-19 vaccine were selected. Blood samples were collected before injection of the vaccine, 3–5 days after the first dose injection, and 3–5 days subsequent to the second vaccination. To check the serum level of CCL2, CCL3, and IL-29, ELISA technique was used.</p></div><div><h3>Results</h3><p>Our results indicated that the serum levels of CCL2, CCL3, and IL-29 were significantly higher after first and second dose of vaccination compared to before vaccine administration. Furthermore, serum levels of all these mediators were higher after second dose of vaccine compared to the first vaccine administration.</p></div><div><h3>Conclusions</h3><p>Oxford–AstraZeneca COVID-19 vaccine is able to induce inflammatory CCL2 and CCL3 chemokines as well as protective interferon lambda (IL-29).</p></div>","PeriodicalId":13270,"journal":{"name":"Immunobiology","volume":"229 2","pages":"Article 152789"},"PeriodicalIF":2.8,"publicationDate":"2024-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S017129852400007X/pdfft?md5=48cdf5d1950de614021469356fe3ada8&pid=1-s2.0-S017129852400007X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139590357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperglycemia modulates M1/M2 macrophage polarization in chronic diabetic patients with pulmonary tuberculosis infection","authors":"Sudhasini Panda ,&nbsp;Alisha Arora ,&nbsp;Kalpana Luthra ,&nbsp;Anant Mohan ,&nbsp;Naval K Vikram ,&nbsp;Neeraj Kumar Gupta ,&nbsp;Archana Singh","doi":"10.1016/j.imbio.2024.152787","DOIUrl":"10.1016/j.imbio.2024.152787","url":null,"abstract":"<div><p>Increased susceptibility to bacterial infections like tuberculosis (TB) is one of the complications of type 2 diabetes, however the underlying mechanisms remains poorly characterized. To explore how chronic hyperglycemia in diabetes affects progression of active TB, we examined mRNA expression of M1 (proinflammatory) and M2 (anti-inflammatory) cytokines/markers, in monocyte-derived macrophages obtained from patients with PTB + DM (pulmonary TB + diabetes mellitus type 2), patients with DM alone, patients with PTB alone, and healthy individuals (controls). Our findings indicate a dysregulated cytokine response in patients with both PTB and DM, characterized by decreased expression levels of interferon-gamma (IFN-γ) and inducible nitric oxide synthase (iNOS), along with increased expression levels of interleukin-1 beta (IL-1β) and CD206. Furthermore, we observed a positive correlation of IL-1β and CD206 expression with levels of glycosylated hemoglobin (HbA1c) in both PTB + DM and DM groups, while IFN-γ showed a positive correlation with HbA1c levels, specifically in the PTB + DM group. Additionally, M1 cytokines/markers, IL-1β and iNOS were found to be significantly associated with the extent of sputum positivity in both PTB and PTB + DM groups, suggesting it to be a function of increased bacterial load and hence severity of infection. Our data demonstrates that tuberculosis in individuals with PTB + DM is characterized by altered M1/M2 cytokine responses, indicating that chronic inflammation associated with type 2 diabetes may contribute to increased immune pathology and inadequate control of tuberculosis infection.</p></div>","PeriodicalId":13270,"journal":{"name":"Immunobiology","volume":"229 2","pages":"Article 152787"},"PeriodicalIF":2.8,"publicationDate":"2024-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0171298524000056/pdfft?md5=71e1a0429a554537b9343ade21ed6d95&pid=1-s2.0-S0171298524000056-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139458950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical relevance of ORCs in predicting prognosis and immunotherapy outcomes: A pan-cancer analysis","authors":"Yajun Zhao ,&nbsp;Jiangtao Wang ,&nbsp;Xiaodong Liang ,&nbsp;Chen Wang","doi":"10.1016/j.imbio.2024.152783","DOIUrl":"https://doi.org/10.1016/j.imbio.2024.152783","url":null,"abstract":"<div><h3>Background</h3><p>The origin recognition complex (ORC) consists of six subunits and mediates DNA replication by binding to its origin. Recent studies show that ORCs are closely related to various biological processes in tumors. However, a comprehensive study of ORCs in pan-cancer has not been conducted.</p></div><div><h3>Results</h3><p>A systematic evaluation of the expression, mutation, and prognostic significance of ORCs was conducted across cancer types using data from The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) databases. The single-sample Gene Set Enrichment Analysis (ssGSEA) was performed using R package “Gene Set Variation Analysis (GSVA)” to evaluate ORC score. ORC score was significantly elevated in most cancers and linked with an inferior prognosis. It was positively related to the G2/M checkpoint and DNA repair pathways. An elevated ORC score also correlated with tumor mutation burden (TMB)/ microsatellite instability (MSI). A prognosis analysis suggested that high ORC scores were associated with heightened immunotherapeutic sensitivity.</p></div><div><h3>Conclusions</h3><p>Our research elucidates the genomic changes associated with and clinical relevance of ORCs in cancer and provides unique insights for future investigation of ORCs in immunotherapy.</p></div>","PeriodicalId":13270,"journal":{"name":"Immunobiology","volume":"229 2","pages":"Article 152783"},"PeriodicalIF":2.8,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0171298524000019/pdfft?md5=1bca731bc80cb958912565d9382ca5e8&pid=1-s2.0-S0171298524000019-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139419213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}