Immunobiology最新文献

{"title":"PRICKLE1 gene methylation and abnormal transcription in Chinese patients with ankylosing spondylitis","authors":"Tingting Zhou ,&nbsp;Xinqi Wang ,&nbsp;Jiangping Kong ,&nbsp;Lingxiang Yu ,&nbsp;Huimin Xie ,&nbsp;Feier Wang ,&nbsp;Shenqian Xu ,&nbsp;Zongwen Shuai ,&nbsp;Qiang Zhou ,&nbsp;Faming Pan","doi":"10.1016/j.imbio.2023.152742","DOIUrl":"10.1016/j.imbio.2023.152742","url":null,"abstract":"<div><h3>Background</h3><p>Ankylosing spondylitis (AS) is a common inflammatory arthritis without a reliable biomarker. The role of methylation and mRNA expression of PRICKLE1 promoter in the pathogenesis of ankylosing spondylitis remains unclear.</p></div><div><h3>Methods</h3><p>A two-stage case-control design was used to detect the characteristics of methyl group and transcriptome of PRICKLE1 gene in Ankylosing spondylitis. The methylation degree of PRICKLE1 gene promoter region was tested by phosphate-sequencing, and further analyzed whether there was significant difference in methylation level of PRICKLE1 gene. The expression levels of PRICKLE1 mRNA in 50 AS patients and 50 healthy controls were detected by real-time quantitative PCR (RT-qPCR).</p></div><div><h3>Results</h3><p>Compared with healthy control group, the intensity of methylation in 4 ponds of PRICKLE1 in patients with Ankylosing spondylitis was low, and the mRNA levels were overexpressed (<em>P</em> = 0.017). ROC results showed that the sensitivity of PRICKLE1 was 68.67% and specificity was 71.43%.</p></div><div><h3>Conclusion</h3><p>There is a significant change in the concentration of serum PRICKLE1 mRNA​in patients with Ankylosing spondylitis, and the degree of gene methylation is significantly reduced, suggesting that PRICKLE1 gene maybe involved in the pathogenesis of Ankylosing spondylitis, which may be useful for predicting the occurrence of AS and finding new early screening indicators.</p></div>","PeriodicalId":13270,"journal":{"name":"Immunobiology","volume":"228 6","pages":"Article 152742"},"PeriodicalIF":2.8,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41127444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cafeteria diet-induced obesity remodels immune response in acute Trypanosoma cruzi infection","authors":"Amanda Goulart ,&nbsp;Naira Ferreira Anchieta ,&nbsp;Pedro Alexandre Sampaio ,&nbsp;Vânia Brazão ,&nbsp;Jefferson Luiz Da Silva ,&nbsp;Gisele Bulhões Portapilla ,&nbsp;Andressa Duarte ,&nbsp;Daiane Yukie Tezuca ,&nbsp;Maiara Voltarelli Providello ,&nbsp;Angelita Maria Stabile ,&nbsp;José Clóvis do Prado Júnior","doi":"10.1016/j.imbio.2023.152747","DOIUrl":"10.1016/j.imbio.2023.152747","url":null,"abstract":"<div><h3>Background</h3><p>Obesity is a global problem associated with several conditions, including hypertension, diabetes, arthritis and cardiovascular diseases. With the increase in the prevalence of obesity in recent years, mostly in developing countries, it is important to study its impact on various diseases, including infectious illnesses, such as Chagas disease, caused by the protozoan <em>Trypanosoma cruzi</em>. Considering that a diet rich in salt, sugar, and fat is associated with obesity, this study aimed to evaluate the influence of cafeteria diet (CAF)-induced obesity on immune responses in <em>T. cruzi</em>-infected rats.</p></div><div><h3>Methods</h3><p>Male <em>Wistar</em> Hannover rats were provided with water and food <em>ad libitum</em> (chow group). The CAF-fed groups received a normal rodent diet or CAF. The animals were intraperitoneally infected with 10⁵ trypomastigote forms of the Y strain of <em>T. cruzi</em> present in the whole blood from a previously infected mouse.</p></div><div><h3>Results</h3><p>CAF-fed rats showed a significant increase in visceral adipose tissue weight compared to chow-fed rats. A significant reduction in CD3⁺ CD4⁺ helper splenic T cells was observed in obese-infected rats compared to non-obese-infected rats, as well as CD11b and macrophages. In addition, macrophages from obese animals displayed reduced RT1b levels compared to those from control animals. Moreover, INF-γ, an important factor in macrophage activation, was reduced in obese-infected rats compared with their counterparts.</p></div><div><h3>Conclusions</h3><p>These results indicate that a CAF can impair the cell-mediated immune response against <em>T. cruzi.</em></p></div>","PeriodicalId":13270,"journal":{"name":"Immunobiology","volume":"228 6","pages":"Article 152747"},"PeriodicalIF":2.8,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41126686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Levels of soluble TNF receptors (sTNFR1 and sTNFR2) increase with clinical worsening of patients and are related to COVID-19 mortality","authors":"Melayne Rocha Aciole ,&nbsp;Juliana Prado Gonçales ,&nbsp;Patrícia Areias Feitosa Neves ,&nbsp;Cynthia Regina Pedrosa Soares ,&nbsp;Marta Iglis de Oliveira ,&nbsp;Heloisa Ramos Lacerda de Melo ,&nbsp;Reginaldo Gonçalves de Lima Neto ,&nbsp;Líbia Cristina Rocha Vilela Moura ,&nbsp;Paulo Sergio Ramos Araújo ,&nbsp;Virginia Maria Barros de Lorena","doi":"10.1016/j.imbio.2023.152748","DOIUrl":"10.1016/j.imbio.2023.152748","url":null,"abstract":"<div><p>The present study aimed to inspect the serum levels of the soluble receptors, sTNFR1 and sTNFR2, in patients with COVID-19. The large production of inflammatory cytokines is an essential process in the pathogenesis of COVID-19. TNF is a multifaceted proinflammatory cytokine which has soluble and membrane receptors. Thus, knowing the role of these receptors will help better understand this disease's immunopathogenesis. We included 131 patients confirmed for SARS-CoV-2, separated into three groups: ward patients without O2 support, group A (n = 14); ward patients with O2 support, group B (n = 85), and patients in an intensive care unit (ICU), group C (n = 32), making up the receptors dosed by flow cytometry. The results showed that sTNFR1 and sTNFR2 are associated with disease severity, being higher in group C when compared to group A. As for the levels of receptors and their relationship with the degree of lung involvement, we found higher values of sTNFR1 in patients in group 1 (pulmonary involvement &lt; 25%), suggesting that inflammatory processes related to TNF are not necessarily associated with the primary site of infection. When we analysed the patients who passed away compared to those who recovered, both receptors significantly increased the mortality numbers. These findings suggest a relevant influence of soluble receptors in the inflammatory processes involved in the pathogenesis of COVID-19. Wherefore, we suggest using these receptors as biomarkers of severity and mortality of the disease.</p></div>","PeriodicalId":13270,"journal":{"name":"Immunobiology","volume":"229 1","pages":"Article 152748"},"PeriodicalIF":2.8,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0171298523045503/pdfft?md5=9cc98f1f35933cadcff9f9c456d6c2c0&pid=1-s2.0-S0171298523045503-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135346641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Moderate and high-intensity interval training protect against diabetes-induced modulation of hepatic CD86 and CD206 expression associated with the amelioration of insulin resistance and inflammation in rats","authors":"Reza Sheikh ,&nbsp;Saeid Shakerian ,&nbsp;Seyed Reza Fatemi Tabatabaei ,&nbsp;Abdolhamid Habibi","doi":"10.1016/j.imbio.2023.152745","DOIUrl":"10.1016/j.imbio.2023.152745","url":null,"abstract":"<div><p><span><span><span>Diabetes Mellitus (DM) can damage the function of metabolic tissues, including the liver. Liver macrophages are the first responders to tissue damage or exercise. We sought to determine whether eight weeks of interval training (HIIT &amp; MIIT) protect against diabetes-induced modulation of hepatic CD86 and CD206 expression associated with the amelioration of insulin resistance and inflammation in rats. Thirty rats were divided into six groups, including a control group, MIIT, HIIT, DM, DM + MIIT, and DM + HIIT (n = 5 in each group). Diabetes was induced using a combination of a high-fat diet (HFD) and STZ. </span>Wistar rats<span> in the exercise groups were subjected to moderate and high-intensity interval training for eight weeks. After sample collection, liver tissue was removed and weighed. Serum levels of TNFα, IL-6, TGFβ, and IL-10 were measured by ELISA. </span></span>Protein expression of the immune markers CD86 and CD206 in liver tissue was determined by immunohistochemical staining. Induction of diabetes increased </span>glycemic indices<span>, insulin resistance, and liver injury enzymes, especially in DM and DM + HIIT groups (p &lt; 0.05). Moreover, diabetic groups showed an increase in liver CD86 protein expression, an increase in TNFα, IL-6, and TGFβ serum levels, and a decrease in liver CD206 and serum IL-10 (p &lt; 0.05). Doing exercise while being diabetic, especially MIIT, significantly reversed the aforementioned factors and reduced insulin resistance (p &lt; 0.05), except IL-10). We concluded that performing exercise training specially MIIT by decreasing CD86 and increasing CD206 in the liver, followed by decreasing pro-inflammatory factors (TNFα, IL-6) caused the regulation of liver enzymes and insulin resistance in diabetic rats. Therefore, it seems that exercise training by regulating macrophage markers CD86 and CD206 can reduce damage to the insulin-signaling pathway by reducing pro-inflammatory cytokines.</span></p></div>","PeriodicalId":13270,"journal":{"name":"Immunobiology","volume":"228 6","pages":"Article 152745"},"PeriodicalIF":2.8,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10312984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluid shear stress enhances dendritic cell activation","authors":"Jenna A. Dombroski,&nbsp;Schyler J. Rowland,&nbsp;Abigail R. Fabiano,&nbsp;Samantha V. Knoblauch,&nbsp;Jacob M. Hope,&nbsp;Michael R. King","doi":"10.1016/j.imbio.2023.152744","DOIUrl":"10.1016/j.imbio.2023.152744","url":null,"abstract":"<div><p>Ex vivo activation of dendritic cells (DCs) has been widely explored for targeted therapies, although these treatments remain expensive. Reducing treatment costs while enhancing cell activation could help to make immunotherapies more accessible. Cells can be activated by both internal and external forces including fluid shear stress (FSS). FSS activates cells via opening of mechanosensitive ion channels. In this study, dendritic cells were activated by sustained exposure to circulatory levels of fluid shear stress using a cone-and-plate flow device and analyzed for activation markers. After 1 h of shear stress exposure, an increase in cytokine release was present in immortalized cells as well as phosphorylation of the proteins NF-κB and cFos in primary DCs. Changes in DC morphology, metabolism and proliferation were also observed. These compelling new findings point to the potential for using FSS to activate DCs for ex vivo therapeutics.</p></div>","PeriodicalId":13270,"journal":{"name":"Immunobiology","volume":"228 6","pages":"Article 152744"},"PeriodicalIF":2.8,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41119113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting C5a is beneficial in critically ill COVID-19 patients","authors":"Endry H.T. Lim ,&nbsp;Alexander P.J. Vlaar ,&nbsp;Matthijs C. Brouwer ,&nbsp;Diederik van de Beek","doi":"10.1016/j.imbio.2023.152743","DOIUrl":"10.1016/j.imbio.2023.152743","url":null,"abstract":"","PeriodicalId":13270,"journal":{"name":"Immunobiology","volume":"228 6","pages":"Article 152743"},"PeriodicalIF":2.8,"publicationDate":"2023-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10634054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"89 Bi-functional C5 antibody-fusion protein (LP-005) with potential best-in-class bioactivity for complement inhibition","authors":"Heng Liu,&nbsp;Haili Ma,&nbsp;Hongzhou Yang,&nbsp;Yunhua Liu,&nbsp;Ruowen Guo,&nbsp;Ming Xie","doi":"10.1016/j.imbio.2023.152540","DOIUrl":"10.1016/j.imbio.2023.152540","url":null,"abstract":"","PeriodicalId":13270,"journal":{"name":"Immunobiology","volume":"228 5","pages":"Article 152540"},"PeriodicalIF":2.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42547476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"164 Role of nutraceuticals in ameliorating inflammation in Alzheimer[StQuote]s disease","authors":"J. Alruwaili, T. Hughes","doi":"10.1016/j.imbio.2023.152614","DOIUrl":"https://doi.org/10.1016/j.imbio.2023.152614","url":null,"abstract":"","PeriodicalId":13270,"journal":{"name":"Immunobiology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44346333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"101 CD59 isoforms IRIS-1 and IRIS-2 are expressed in human and mouse brains, required for neurotransmitter release, and have a decreased expression in Alzheimer[StQuote]s disease patient[StQuote]s brains","authors":"Ewelina Golec ,&nbsp;Yasmin Serjieh ,&nbsp;Maja Karlsson ,&nbsp;Ben C. King ,&nbsp;Malin Wennström ,&nbsp;Anna M. Blom","doi":"10.1016/j.imbio.2023.152552","DOIUrl":"10.1016/j.imbio.2023.152552","url":null,"abstract":"","PeriodicalId":13270,"journal":{"name":"Immunobiology","volume":"228 5","pages":"Article 152552"},"PeriodicalIF":2.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48518375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"112 Distinctive dosage requirements between C3 and Factor D (FD) in the activation of the alternative complement pathway","authors":"Xiaobo Wu,&nbsp;Zheng Hu,&nbsp;Thomas Xu,&nbsp;John Atkinson","doi":"10.1016/j.imbio.2023.152563","DOIUrl":"10.1016/j.imbio.2023.152563","url":null,"abstract":"","PeriodicalId":13270,"journal":{"name":"Immunobiology","volume":"228 5","pages":"Article 152563"},"PeriodicalIF":2.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46330924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}