Immunochemistry最新文献_第5页

Immunochemical studies of the a allotypes of rabbit heavy chain variable regions—II 兔重链可变区- ii的a型异体免疫化学研究

Immunochemistry Pub Date : 1978-08-01 DOI: 10.1016/0161-5890(78)90010-X

Aftab A. Ansari , Rose G. Mage , M. Carta-Sorcini , S. Carta , E. Appella

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引用次数: 3

Comparison by1H NMR of the hapten environment in the combining site of the dinitrophenyl binding IgA protein 315 and its fragments 二硝基苯结合IgA蛋白315及其片段结合位点半抗原环境的1h NMR比较

Immunochemistry Pub Date : 1978-08-01 DOI: 10.1016/0161-5890(78)90002-0

Arabella T. Morris , David Givol , Raymond A. Dwek

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引用次数: 3

Antibodies to poly d(A-T) and their reactions with oligonucleotides and nucleic acids 聚d抗体及其与寡核苷酸和核酸的反应

Immunochemistry Pub Date : 1978-08-01 DOI: 10.1016/0161-5890(78)90040-8

Beverly W. Lubit , Bernard F. Erlanger

{"title":"Antibodies to poly d(A-T) and their reactions with oligonucleotides and nucleic acids","authors":"Beverly W. Lubit , Bernard F. Erlanger","doi":"10.1016/0161-5890(78)90040-8","DOIUrl":"10.1016/0161-5890(78)90040-8","url":null,"abstract":"<div>Antibodies to poly d(A-T)‡ were elicited in two rabbits by immunization with complexes of heat-denatured poly d(A-T) and MBSA. Denaturation of poly d(A-T) was carried out in a salt solution and in distilled water. The antibodies were characterized by precipitation, hemagglutination and radioimmunoassay procedures. The antibody bound poly d(A-T) and cross reactions were observed with dAdo, dThd, poly(b) and ApU but not with UpA.V.faba satellite and calf thymus DNAs were bound at least as well as poly d(A-T); DNA fromB. subtilis andPseudomonas were poorly bound. After absorption of the serum with Ado-RSA, Thd-RSA and BSA, the antisera were directed primarily to heat-denatured poly d(A-T). Cross-reactions were observed with mouse myeloma DNA, poly(dT), poly(dA)-poly(dT) but not with poly(dA). The results indicated that anti-poly d(A-T) recognizes sequence and secondary structures. The immunochemical data are in accord with findings made by other workers on DNA and synthetic polynucleotides by physical studies, such as X-ray diffraction and scattering. Moreover, the immunochemical data support earlier contentions that local conformations in nucleic acids are responsive to primary sequences as is the case with proteins.Also described are procedures for the synthesis of poly d(A-T)-[3H]A and poly ([3H]A-T). For the preparation of the latter, TTP was synthesized from TMP which was prepared enzymically from Thd.</div>","PeriodicalId":13265,"journal":{"name":"Immunochemistry","volume":"15 9","pages":"Pages 663-671"},"PeriodicalIF":0.0,"publicationDate":"1978-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0161-5890(78)90040-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11938768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Meeting report: First international symposium on immunobiology of proteins and peptides 会议报告:首届国际蛋白质和多肽免疫生物学研讨会

Immunochemistry Pub Date : 1978-08-01 DOI: 10.1016/0161-5890(78)90015-9

M. Atassi, E. Benjamini, J. Goodman, A. Stavitsky