D. Bout, H. Dupas, M. Capron, A. El Gazawi , Y. Carlier, A. Delacourte , A. Capron
{"title":"曼氏血吸虫苹果酸脱氢酶的纯化、免疫化学及生物学特性研究","authors":"D. Bout, H. Dupas, M. Capron, A. El Gazawi , Y. Carlier, A. Delacourte , A. Capron","doi":"10.1016/0161-5890(78)90036-6","DOIUrl":null,"url":null,"abstract":"<div><p>Affinity chromatography with AMP-Sepharose allows us to purify malate dehydrogenase of<em>Schistosoma mansoni</em> and to perform the studies about the immunochemical and biological properties of this enzyme. MDH has a mol. wt 60,000 and has two isoenzymes (pI 7.1 and 8.5), which are antigenically different. Isoenzyme (pI 7.1) is the antigen called 4, specific of the genus<em>Schistosoma</em> and is highly immunogenic. By immunofluorescence, MDH was localized in the cellular layer of the coecum. Mice immunized with purified MDH exhibited a significant decrease of worm burden.</p></div>","PeriodicalId":13265,"journal":{"name":"Immunochemistry","volume":"15 9","pages":"Pages 633-638"},"PeriodicalIF":0.0000,"publicationDate":"1978-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0161-5890(78)90036-6","citationCount":"16","resultStr":"{\"title\":\"Purification, immunochemical and biological characterization of malate dehydrogenase ofSchistosoma mansoni\",\"authors\":\"D. Bout, H. Dupas, M. Capron, A. El Gazawi , Y. Carlier, A. Delacourte , A. Capron\",\"doi\":\"10.1016/0161-5890(78)90036-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Affinity chromatography with AMP-Sepharose allows us to purify malate dehydrogenase of<em>Schistosoma mansoni</em> and to perform the studies about the immunochemical and biological properties of this enzyme. MDH has a mol. wt 60,000 and has two isoenzymes (pI 7.1 and 8.5), which are antigenically different. Isoenzyme (pI 7.1) is the antigen called 4, specific of the genus<em>Schistosoma</em> and is highly immunogenic. By immunofluorescence, MDH was localized in the cellular layer of the coecum. Mice immunized with purified MDH exhibited a significant decrease of worm burden.</p></div>\",\"PeriodicalId\":13265,\"journal\":{\"name\":\"Immunochemistry\",\"volume\":\"15 9\",\"pages\":\"Pages 633-638\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1978-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0161-5890(78)90036-6\",\"citationCount\":\"16\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Immunochemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0161589078900366\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Immunochemistry","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0161589078900366","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Purification, immunochemical and biological characterization of malate dehydrogenase ofSchistosoma mansoni
Affinity chromatography with AMP-Sepharose allows us to purify malate dehydrogenase ofSchistosoma mansoni and to perform the studies about the immunochemical and biological properties of this enzyme. MDH has a mol. wt 60,000 and has two isoenzymes (pI 7.1 and 8.5), which are antigenically different. Isoenzyme (pI 7.1) is the antigen called 4, specific of the genusSchistosoma and is highly immunogenic. By immunofluorescence, MDH was localized in the cellular layer of the coecum. Mice immunized with purified MDH exhibited a significant decrease of worm burden.
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