Histopathology最新文献

{"title":"Stroma areactive invasion front areas (SARIFA) in 1,298 pT3/pT4 colorectal cancers: A strong prognostic parameter complementing established morphological criteria","authors":"Sebastian Foersch,&nbsp;Nuwar Harb,&nbsp;Anne-Sophie Litmeyer,&nbsp;Wilfried Roth,&nbsp;Irina Breus,&nbsp;Johannes Schraml,&nbsp;Annika Weiß,&nbsp;Katja Steiger,&nbsp;Julia Teply-Szymanski,&nbsp;Detlef K Bartsch,&nbsp;Carsten Denkert,&nbsp;Maxime Schmitt,&nbsp;Moritz Jesinghaus","doi":"10.1111/his.15501","DOIUrl":"10.1111/his.15501","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p>Colorectal carcinoma (CRC) is one of the most common cancers worldwide and is associated with significant morbidity and mortality. Histopathology plays a crucial role in the diagnosis, prognostication and treatment planning of CRC. In addition to well-established morphological parameters such as tumour budding, grade and histopathological subtypes, the interaction between tumour cells and adipose tissue has gained increasing attention.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods and results</h3>\u0000 \u0000 <p>The frequency of direct proximity between cancer cells and adipocytes, termed <i>Stroma Areactive Invasion Front Areas</i> (SARIFA), was assessed in a multicentre cohort of 1,298 patients with pT3/pT4 CRC. The prognostic impact of SARIFA was evaluated, with a particular focus on its relationship with established histopathological parameters. SARIFA was associated with adverse clinicopathological features and served as an indicator of poor prognosis across all survival metrics in the overall cohort (disease-specific survival [DSS]; <i>P</i> &lt; 0.001). This prognostic value remained significant across all pTNM stages (e.g. DSS in pT3, pN0, pN1/2, pM0 CRC; <i>P</i> &lt; 0.001, respectively) and identified additional adverse prognostic subgroups within tumour budding and grade categories (DSS, <i>P</i> &lt; 0.001, respectively) and within histopathological subtypes. In multivariable analyses, including pTNM stage and the aforementioned histopathological parameters, SARIFA remained a highly significant prognostic factor (DSS <i>P</i> &lt; 0.001, hazard ratio: 1.73).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Discussion</h3>\u0000 \u0000 <p>Our study confirms the high prognostic relevance of SARIFA across key clinicopathological subgroups of CRC and highlights its value as a meaningful complement to established morphological parameters. Given its ability to provide additional prognostic insights, our findings advocate for its inclusion in pathology reports to improve risk assessment and clinical management.</p>\u0000 </section>\u0000 </div>","PeriodicalId":13219,"journal":{"name":"Histopathology","volume":"87 3","pages":"464-473"},"PeriodicalIF":4.1,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/his.15501","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Featured Cover","authors":"","doi":"10.1111/his.15503","DOIUrl":"https://doi.org/10.1111/his.15503","url":null,"abstract":"<p>The cover image is based on the article <i>ROS1 immunohistochemistry as a potential predictive biomarker for ROS1-targeted therapy in breast cancer: impact of antibody clone selection</i> by Amy McCart Reed et al., https://doi.org/10.1111/his.15465.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":13219,"journal":{"name":"Histopathology","volume":"87 2","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/his.15503","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive genomic profiling and immunohistochemical analysis of prepubertal-type testicular neuroendocrine tumours in postpubertal patients reveal a possible relationship with small intestinal neuroendocrine tumours","authors":"Luca Szalai,&nbsp;Maja Leiner,&nbsp;Erika Tóth,&nbsp;Levente Kuthi,&nbsp;Andrea Kohánka,&nbsp;Krisztina Bíró,&nbsp;Áron Somorácz,&nbsp;Borbála Dénes,&nbsp;Zsombor Melegh","doi":"10.1111/his.15510","DOIUrl":"10.1111/his.15510","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p>Prepubertal-type testicular neuroendocrine tumours are rare entities with a poorly understood genetic background and immunohistochemical properties. Here, we present five cases in which immunohistochemical and molecular studies were performed to better understand the characteristics of these lesions.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods and results</h3>\u0000 \u0000 <p>Between 2006 and 2024, we identified five cases of prepubertal-type testicular neuroendocrine tumours in postpubertal patients with no extragonadal disease. Immunohistochemical studies (synaptophysin, chromogranin, CDX2, SATB2, TTF1, OCT4 and Ki67) were performed on all cases. Synaptophysin and chromogranin showed positive staining in all five cases, while TTF1 and OCT4 reactions were negative. In each case, varying levels of positivity with CDX2 and SATB2 were observed, although SATB2 was predominantly cytoplasmic. INSM1 and SF1 immunohistochemistry were performed on four cases. INSM1 showed a variable degree of positivity in all four cases examined, while SF1 was negative. Proliferative activity, assessed with Ki67 antibody, was below 10% in all cases. Chromosomal status of 12p was assessed by fluorescence in situ hybridization (FISH), and no chromosomal aberrations affecting the short arm of chromosome 12 were identified. In addition, comprehensive genomic profiling (CGP) using Oncomine Comprehensive Assay Plus was performed on two cases. Molecular analysis identified alterations previously described as characteristic of small intestinal neuroendocrine tumours: deletion of chromosome 18 in both cases and <i>CDKN1B</i> mutation in one case.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Immunohistochemistry revealed varying levels of positivity with intestinal markers in our cases. In two cases where CGP was performed, molecular alterations previously described as characteristic of small intestinal neuroendocrine tumours were identified. These findings suggest intestinal differentiation in these tumours and a possible relationship to small intestinal neuroendocrine tumours. This could also pose differential diagnostic challenges. Therefore, investigation of the gastrointestinal system is recommended in such cases to exclude metastatic lesions.</p>\u0000 </section>\u0000 </div>","PeriodicalId":13219,"journal":{"name":"Histopathology","volume":"87 5","pages":"700-706"},"PeriodicalIF":4.1,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A deep active learning framework for mitotic figure detection with minimal manual annotation and labelling","authors":"Eric Liu,&nbsp;August Lin,&nbsp;Pramath Kakodkar,&nbsp;Yayuan Zhao,&nbsp;Boyu Wang,&nbsp;Charles Ling,&nbsp;Qi Zhang","doi":"10.1111/his.15506","DOIUrl":"10.1111/his.15506","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p>Accurately and efficiently identifying mitotic figures (MFs) is crucial for diagnosing and grading various cancers, including glioblastoma (GBM), a highly aggressive brain tumour requiring precise and timely intervention. Traditional manual counting of MFs in whole slide images (WSIs) is labour-intensive and prone to interobserver variability. Our study introduces a deep active learning framework that addresses these challenges with minimal human intervention.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods and results</h3>\u0000 \u0000 <p>We utilized a dataset of GBM WSIs from The Cancer Genome Atlas (TCGA). Our framework integrates convolutional neural networks (CNNs) with an active learning strategy. Initially, a CNN is trained on a small, annotated dataset. The framework then identifies uncertain samples from the unlabelled data pool, which are subsequently reviewed by experts. These ambiguous cases are verified and used for model retraining. This iterative process continues until the model achieves satisfactory performance. Our approach achieved 81.75% precision and 82.48% recall for MF detection. For MF subclass classification, it attained an accuracy of 84.1%. Furthermore, this approach significantly reduced annotation time - approximately 900 min across 66 WSIs - cutting the effort nearly in half compared to traditional methods.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Our deep active learning framework demonstrates a substantial improvement in both efficiency and accuracy for MF detection and classification in GBM WSIs. By reducing reliance on large annotated datasets, it minimizes manual effort while maintaining high performance. This methodology can be generalized to other medical imaging tasks, supporting broader applications in the healthcare domain.</p>\u0000 </section>\u0000 </div>","PeriodicalId":13219,"journal":{"name":"Histopathology","volume":"87 4","pages":"536-547"},"PeriodicalIF":4.1,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12445828/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Repetitive invasion does not preclude the possibility of invasive-type intraductal carcinoma of the prostate","authors":"Murali Varma,&nbsp;Glen Kristiansen,&nbsp;Ming Zhou","doi":"10.1111/his.15508","DOIUrl":"10.1111/his.15508","url":null,"abstract":"","PeriodicalId":13219,"journal":{"name":"Histopathology","volume":"87 5","pages":"778-779"},"PeriodicalIF":4.1,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Renal cell carcinoma with fibromyomatous stroma (RCC FMS) and with hemangioblastoma-like areas is part of the RCC FMS spectrum in patients with tuberous sclerosis complex","authors":"Katherina Baranova,&nbsp;Jacob A Houpt,&nbsp;Deaglan Arnold,&nbsp;Andrew A House,&nbsp;Laura Lockau,&nbsp;Lindsay Ninivirta,&nbsp;Stephen Pautler,&nbsp;Haiying Chen,&nbsp;Madeleine Moussa,&nbsp;Rola Saleeb,&nbsp;Jose A Gomez,&nbsp;Asli Yilmaz,&nbsp;Farshid Siadat,&nbsp;Adrian Box,&nbsp;Douglas J Mahoney,&nbsp;Franz J Zemp,&nbsp;Manal Gabril,&nbsp;Kiril Trpkov","doi":"10.1111/his.15505","DOIUrl":"10.1111/his.15505","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p>Sporadic renal cell carcinomas with fibromyomatous stroma (RCC FMS) with coexistent haemangioblastoma (HB)-like morphology have been previously reported. Such morphology has not, however, been documented in patients with tuberous sclerosis complex (TSC). We evaluated clinicopathologic, immunohistochemical and molecular findings of RCC FMS with HB-like features in three TSC patients.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods and results</h3>\u0000 \u0000 <p>All three patients were females with confirmed germline mutations in <i>TSC1</i> in the absence of <i>VHL</i> or other alterations. One patient had bilateral nephrectomy with six separate tumours (three in each kidney), and two patients had one tumour each, resected by partial nephrectomy. Prominent fibromuscular stroma was present at the tumoral periphery and surrounding clear cell nests forming tubules and papillary formations (RCC FMS areas), admixed with solid, clear to eosinophilic and spindle cell areas (HB-like areas). HB-like areas were variably represented in individual tumours, but in some, HB-like features were almost exclusive. HB-like areas were negative for CK7 and showed inhibin-α and S100 reactivity, in contrast to typical RCC FMS areas, which were CK7 positive, while negative for inhibin-α and S100. GPNMB, which is consistently expressed in <i>TSC/mTOR</i> altered tumours, was positive in both components. On follow-up (10 months–21 years), all patients had an indolent course.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>HB-like morphology found in RCC FMS in TSC patients represents part of the morphologic spectrum of RCC FMS, which is a previously underreported and underrecognized feature. RCC FMS with HB-like areas show uniform GPNMB reactivity and are associated with <i>TSC/MTOR</i> alterations, but not with <i>VHL</i> alterations.</p>\u0000 </section>\u0000 </div>","PeriodicalId":13219,"journal":{"name":"Histopathology","volume":"87 5","pages":"687-699"},"PeriodicalIF":4.1,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/his.15505","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Loss of MTAP expression is not an accurate surrogate for CDKN2A homozygous deletions in peritoneal mesothelioma","authors":"Andrea Quaranta,&nbsp;Andrea Marzullo,&nbsp;Francesco Fortarezza,&nbsp;Sonia Maniglio,&nbsp;Concetta Caporusso,&nbsp;Floriana Pentimone,&nbsp;Federica Pezzuto,&nbsp;Domenica Cavone,&nbsp;Teresa Lettini,&nbsp;Mario Magistro,&nbsp;Cecilia Salzillo,&nbsp;Luigi Vimercati,&nbsp;Paolo Graziano,&nbsp;Gabriella Serio,&nbsp;Antonio d'Amati","doi":"10.1111/his.15502","DOIUrl":"10.1111/his.15502","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p>Mesothelioma is a malignant neoplasm of the serosal membranes originating from mesothelial cells. Peritoneal mesothelioma is the second most common mesothelial neoplasm after pleural mesothelioma, accounting for approximately 6%–15% of cases. Due to its high morphological variability, often mimicking other lesions, mesothelioma remains a diagnostic challenge. <i>CDKN2A</i> homozygous deletion has been established as a highly accurate biomarker for differentiating mesothelioma from benign mesothelial proliferations. MTAP immunohistochemistry (IHC) has been proposed as a cheaper and more reproducible surrogate for <i>CDKN2A</i> homozygous deletion (HD) detected by FISH in pleural mesothelioma. The aim of our study was to evaluate the reliability of MTAP IHC as a surrogate marker for <i>CDKN2A</i> HD in peritoneal mesothelioma.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods and results</h3>\u0000 \u0000 <p>Thirty-nine FFPE tissue samples of PeM were analysed for <i>CDKN2A</i> copy number status by FISH. MTAP IHC was performed using antibody clone 2G4, and cytoplasmic positivity was evaluated using two different cut-offs (1% and 30%). Agreement between IHC and FISH was assessed using Cohen's Kappa. A ROC curve analysis was performed to evaluate the overall diagnostic performance of MTAP IHC. McNemar's test was used to identify statistically significant discordance between the techniques, and a power analysis was conducted to confirm the adequacy of the sample size. Additionally, 14 benign peritoneal lesions were included as external controls and underwent both FISH and IHC. All control samples showed preserved MTAP expression and no <i>CDKN2A</i> deletion. <i>CDKN2A</i> HD was detected in 27/39 cases. MTAP loss was observed in 13 cases, while the remaining 26 cases showed variable levels of MTAP positivity (15%–100%; mean: 36.4%; median: 25%). Cohen's Kappa revealed a low, non-significant concordance between MTAP IHC and <i>CDKN2A</i> HD (cut-off 1%: Kappa = 0.091, <i>P</i> = 0.462; cut-off 30%: Kappa = 0.083, <i>P</i> = 0.326). ROC curve analysis (AUC = 0.569) confirmed the poor discriminatory performance of MTAP IHC. McNemar's test showed a statistically significant discordance between MTAP IHC and <i>CDKN2A</i> FISH results. Power analysis confirmed that the sample size (<i>n</i> = 39) was adequate.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>These findings may reflect biological and pathogenetic differences between pleural and peritoneal mesotheliomas. Larger, multicentric studies are needed to validate the diagnostic role of MTAP IHC in peritoneal mesothelioma.</p>\u0000 </section>\u0000 </div>","PeriodicalId":13219,"journal":{"name":"Histopathology","volume":"87 4","pages":"526-535"},"PeriodicalIF":4.1,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12445810/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144496096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surgical pathology of diffuse parenchymal lung disease in patients with polymyalgia rheumatica","authors":"Suhashini Ganapaty,&nbsp;Elena Cavazzi,&nbsp;Peter J Manchen,&nbsp;Yasmeen M Butt,&nbsp;Maxwell L Smith,&nbsp;Henry D Tazelaar,&nbsp;Anja C Roden,&nbsp;Brandon T Larsen","doi":"10.1111/his.15483","DOIUrl":"10.1111/his.15483","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p>Polymyalgia rheumatica (PMR) is a chronic autoimmune disorder that mainly affects older adults. Pulmonary disease in PMR is rare but may be under-recognized and pathological descriptions thereof are few. We aimed to characterize diffuse parenchymal lung disease (DPLD) in PMR.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods and results</h3>\u0000 \u0000 <p>Institutional archives were searched for patients having PMR and DPLD with lung tissue sampling. After excluding cases with infection, concomitant rheumatoid arthritis, or smoking-related DPLD only, 11 patients (9 women, median age 75 years) were enrolled. Clinical history and pathology slides were reviewed. One of the 11 patients (9%) had concomitant giant cell arteritis; the remaining patients had no other rheumatological diseases. All had been treated for PMR with immunosuppression, and most presented years later (median 6 years) with non-specific respiratory symptoms. Radiographically, bilateral ground-glass opacities and reticulation were typical and were usually lower lobe predominant. Histologically, fibrosis was seen in 8 of 11 (73%) patients and was unclassifiable in four; non-specific interstitial pneumonia was encountered in three patients, and usual interstitial pneumonia was seen in only one case. Evidence of acute lung injury occurred in 9 (82%) patients, including three with acute lung injury only, usually manifesting as organizing pneumonia. Diffuse alveolar haemorrhage was seen in four cases (29%), including two patients with haemoptysis and capillaritis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Our data corroborate prior reports of clinically significant DPLD in some patients with PMR. Histopathological findings mirror other rheumatological disorders and include diffuse alveolar haemorrhage with capillaritis. Additional studies are warranted to clarify the association between PMR and DPLD.</p>\u0000 </section>\u0000 </div>","PeriodicalId":13219,"journal":{"name":"Histopathology","volume":"87 3","pages":"453-463"},"PeriodicalIF":4.1,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144368828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anal squamous cell carcinoma with mucinous microcysts represents anal gland duct differentiation","authors":"Newton ACS Wong,&nbsp;Guzin Bostanci","doi":"10.1111/his.15500","DOIUrl":"10.1111/his.15500","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p>The subtype of anal cancer known as squamous cell carcinoma with mucinous microcysts (SCC-MM) is recognised to portend a poorer prognosis. However, the clinical significance of identifying this subtype has been downplayed more recently because of the frequent admixture of other SCC subtypes with SCC-MM and subjectivity in diagnosing the latter. This study aimed to immunohistochemically assess an anecdotal observation that SCC-MM morphologically resembles anal gland ducts and to thus determine whether SCC-MM has a unique immunoprofile which could then assist its distinction from differential diagnoses.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods and results</h3>\u0000 \u0000 <p>Ten immunohistochemical markers were applied to five cases of SCC-MM, three cases each of basaloid SCC and conventional SCC of the anus, as well as physiological anal tissue including anal gland ducts and both transitional and squamous epithelia. Of the latter three, the immunophenotype of SCC-MM most resembled anal gland duct epithelium, whereas the immunophenotype of basaloid SCC most resembled transitional epithelium. SCC-MM differed from basaloid SCC by expressing MUC5AC, expressing GATA3 more diffusely and showing only peripheral p63 positivity.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>SCC-MM represents anal gland duct differentiation and can be reliably distinguished from its closest and commonest differential diagnosis, basaloid SCC, by the histological identification of mucin containing cysts and by immunohistochemistry for MUC5AC, GATA3 and p63. This distinction may be clinically important for the prognostication and pathological staging of anal carcinoma in excision specimens.</p>\u0000 </section>\u0000 </div>","PeriodicalId":13219,"journal":{"name":"Histopathology","volume":"87 3","pages":"474-477"},"PeriodicalIF":4.1,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Standardizing the histopathological diagnosis of adenomyosis: an international Delphi consensus","authors":"Tristan McCaughey,&nbsp;Marsali Newman,&nbsp;Leonie Constable,&nbsp;Fatima Figueiredo,&nbsp;Samantha S Mooney,&nbsp;Charlotte Reddington,&nbsp;Helen C McNamara,&nbsp;Amber L Kennedy,&nbsp;Martin Healey","doi":"10.1111/his.15480","DOIUrl":"10.1111/his.15480","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p>To use the Delphi consensus methodology to establish standardized criteria for the histopathological diagnosis of adenomyosis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods and results</h3>\u0000 \u0000 <p>Between April and September 2024, a modified three-round Delphi consensus study was conducted. Thirty-one gynaecological pathologists from 18 countries participated in surveys to evaluate and refine a diagnostic framework for adenomyosis in hysterectomy specimens. Key areas achieving the highest level of agreement included: 4–6 blocks for routine histopathological examination of hysterectomy specimens with benign indications; defining adenomyosis as endometrial glands and/or stroma greater than 2 mm into the myometrium or more than one-third of myometrial thickness; that the absolute number of glands or stromal tissue does not contribute to the diagnosis of adenomyosis; a single gland or stromal focus can be diagnostic; and definitions of focal, extensive, superficial and deep adenomyosis. In total, 93% of respondents were in favour of standardizing the diagnosis to reduce inter-pathologist variability.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study proposes the first consensus-based guideline for the histopathological diagnosis of adenomyosis. Supported by the responses of 31 international experts through a modified Delphi method, this framework provides pathologists with clear diagnostic criteria. Further research should correlate these criteria with clinical symptoms and outcomes.</p>\u0000 </section>\u0000 </div>","PeriodicalId":13219,"journal":{"name":"Histopathology","volume":"87 3","pages":"446-452"},"PeriodicalIF":4.1,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}