Histopathology最新文献

{"title":"Assessment and classification of sex cord-stromal tumours of the testis: recommendations from the testicular sex cord-stromal tumour (TESST) group, an Expert Panel of the Genitourinary Pathology Society (GUPS) and International Society of Urological Pathology (ISUP)","authors":"Andres M Acosta,&nbsp;Maurizio Colecchia,&nbsp;Eva Comperat,&nbsp;Kristine M Cornejo,&nbsp;Anthony J Gill,&nbsp;Sounak Gupta,&nbsp;John C Cheville,&nbsp;Muhammad T Idrees,&nbsp;Chia-Sui Kao,&nbsp;Fiona Maclean,&nbsp;Andres Matoso,&nbsp;Maria Rosaria Raspollini,&nbsp;Kvetoslava Michalova,&nbsp;Miguel Reyes Múgica,&nbsp;Satish K Tickoo,&nbsp;Toyonori Tsuzuki,&nbsp;Thomas M Ulbright,&nbsp;Sean R Williamson,&nbsp;Stephanie Siegmund,&nbsp;Lynette M Sholl,&nbsp;Pilar Gonzalez-Peramato,&nbsp;Daniel M Berney","doi":"10.1111/his.15482","DOIUrl":"10.1111/his.15482","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p>Testicular sex cord-stromal tumours (TSCSTs) are relatively rare, accounting for ~5% of all testicular neoplasms. They were historically classified into Leydig cell tumour, Sertoli cell tumour, granulosa cell tumour, and unclassified sex cord-stromal tumour. More recently, classification was expanded to incorporate additional histologic types, including some associated with inherited cancer predisposition syndromes. However, the classification of TSCSTs still relies entirely on morphology, with some tumour types being defined based on their resemblance to ovarian counterparts. In recent years, molecular studies have identified drivers and genomic alterations associated with aggressive behaviour and progression; however, these findings have not yet impacted classification and management.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods and results</h3>\u0000 \u0000 <p>Under sponsorship of the International Society of Urological Pathology (ISUP) and the Genitourinary Pathology Society (GUPS), a group of genitourinary pathologists was assembled in 2023 with the aim of assessing how to use these new data to improve the classification and management of TSCSTs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>This paper summarizes the recommendations derived from the consensus activities and the first meeting of the <i>te</i>sticular <i>s</i>ex cord-<i>s</i>tromal <i>t</i>umour (TESST) group (held at Johns Hopkins Hospital, Baltimore, USA, 3/23/2024).</p>\u0000 </section>\u0000 </div>","PeriodicalId":13219,"journal":{"name":"Histopathology","volume":"87 5","pages":"660-676"},"PeriodicalIF":4.1,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/his.15482","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144316815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Practical implementation of AI in a non-academic, non-commercial Pathology laboratory: Real world experience and lessons learned","authors":"Frederik Deman,&nbsp;Glenn Broeckx,&nbsp;Sabine Declercq,&nbsp;Quentin Degotte,&nbsp;Jakob Raymaekers,&nbsp;Roberto Salgado,&nbsp;Amélie Dendooven","doi":"10.1111/his.15481","DOIUrl":"10.1111/his.15481","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p>As pathology departments transition towards digital workflows, the integration of artificial intelligence (AI) is anticipated to become increasingly common. This study aimed to describe the real-world implementation and impact of AI integration in routine pathological diagnostics, specifically focusing on prostate biopsy evaluations at the Department of Pathology, ZAS Hospitals, Antwerp.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods and Results</h3>\u0000 \u0000 <p>An AI tool for analysing prostate biopsies was integrated into the department's daily workflow by embedding it into existing laboratory information and reporting systems. Following a short adaptation period, the use of AI led to measurable improvements. Most notably, there was a reduction in the number of immunohistochemical tests required, indicating more confident primary diagnoses. Additionally, a significant decrease in turnaround times for biopsy evaluations was observed, highlighting improved efficiency. The implementation process was closely monitored, and practical insights were gathered to guide future AI deployments in pathology.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The first-year experience of integrating AI into daily pathological practice demonstrated tangible benefits in diagnostic efficiency and workflow optimization. However, the process also revealed several challenges related to real-world deployment, including adaptation by staff and system integration hurdles. The lessons learned provide valuable guidance for other institutions considering similar AI implementations, reinforcing the importance of strategic planning, training and system compatibility in successful adoption.</p>\u0000 </section>\u0000 </div>","PeriodicalId":13219,"journal":{"name":"Histopathology","volume":"87 5","pages":"635-646"},"PeriodicalIF":4.1,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/his.15481","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sarcoma harbouring CEP170::RAD51B fusion: a case report emphasizing diagnostic caution in low-read, pseudogene-associated rearrangements","authors":"Zhengchun Lu,&nbsp;Benjamin F Smith,&nbsp;Kristina M Wakeman,&nbsp;Dustin Shackleton,&nbsp;Jae Elkind,&nbsp;Marcela Riveros Angel,&nbsp;Christopher G Suciu,&nbsp;Tanaya Neff,&nbsp;Carol Beadling,&nbsp;Sintawat Wangsiricharoen","doi":"10.1111/his.15477","DOIUrl":"10.1111/his.15477","url":null,"abstract":"","PeriodicalId":13219,"journal":{"name":"Histopathology","volume":"87 3","pages":"483-485"},"PeriodicalIF":4.1,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methylation analysis as a diagnostic tool for sweat gland tumours classification with emphasis on the distinction between digital papillary adenocarcinoma and mimickers","authors":"Pierre Sohier,&nbsp;Nicolas Macagno,&nbsp;Anne Tallet,&nbsp;Coralie Mousset,&nbsp;Maxime Battistella,&nbsp;Eduardo Calonje,&nbsp;Andreas Von Deimling,&nbsp;Thibault Kervarrec","doi":"10.1111/his.15479","DOIUrl":"10.1111/his.15479","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p>Digital papillary adenocarcinoma (DPA) is a rare sweat gland carcinoma arising on acral sites. The main differential diagnosis included tubular adenoma, hidradenoma, poroid hidradenoma, and mixed tumours, distinction between DPA and these mimickers being crucial for therapeutic management. Recently, HPV42 was identified as the main oncogenic driver of most DPA. Controversially, a few sweat gland tumour cases diagnosed as “DPA” but lacking the HPV42 genome and harbouring instead a <i>BRAF</i>^V600E mutation, a genetic hallmark of tubular adenomas, have been recently described. Methylation analysis is a powerful tool for tumour classification systems. In this context, the aim of the present study is to evaluate the accuracy of methylation analysis for sweat gland tumour classification with special emphasis on the distinction between DPA and mimickers.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods and results</h3>\u0000 \u0000 <p>Twelve DPA, 11 tubular adenomas, 12 hidradenomas, 8 apocrine and 6 eccrine mixed tumours, 7 poromas and 6 adnexal sweat gland carcinoma not otherwise specified (NOS) were submitted for DNA methylation profiling. The results of this analysis show that most of these tumour types formed their own unique cluster, setting them apart from the others. In particular, DPA cases clustered together and were distinct from other tumour entities including tubular adenomas.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Our data support the distinction between DPA and tubular adenomas as two unique entities and further confirm DNA methylation profiling as a relevant tool for tumour classification.</p>\u0000 </section>\u0000 </div>","PeriodicalId":13219,"journal":{"name":"Histopathology","volume":"87 4","pages":"618-623"},"PeriodicalIF":4.1,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12445876/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144266057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epithelial elements in superficial angiomyxomas: mimicry of adnexal development, and mesenchymal-to-epithelial transition","authors":"Carlos Monteagudo,&nbsp;Liria Terrádez,&nbsp;Esther Alvarez,&nbsp;Paloma Masiá,&nbsp;Mónica Espino,&nbsp;María Ortega","doi":"10.1111/his.15478","DOIUrl":"10.1111/his.15478","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p>A subset of superficial angiomyxomas contains epithelial elements. Rarely, especially in Carney's complex patients, arborizing adnexal proliferations are present, with some authors proposing that they could be authentic adnexal neoplasms. Our aim was to determine if the epithelial elements are trapped adnexal epithelium, epithelial outgrowths triggered by myxoid tumour cells or genuinely neoplastic.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods and results</h3>\u0000 \u0000 <p>We studied the clinicopathological and immunophenotypic features (immunohistochemistry and double immunofluorescence) of 43 superficial angiomyxomas, including one from a Carney's complex patient. We found hair follicle placode/germ formation in 7 cases; elongated and/or branched eccrine elements in 4 cases and solid/cystic infundibular-like hyperplastic epithelium in 10 cases. S100A4-positive mesenchymal condensates surrounded follicular and eccrine epithelial buds close to myxoid tumour cells, but only the latter showed variable PRKAR1A loss. In the Carney's complex case, prominent eccrine duct branching was found mimicking fibrofolliculoma, with vimentin-positive cell aggregates connecting and integrating with the pre-existing epithelium. By double immunofluorescence, they showed cytokeratin and E-cadherin immunoreactivity while still expressing vimentin as evidence of mesenchymal-to-epithelial transition.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Our results suggest that adnexal epithelial elements reproduce their embryogenesis through the involvement of S100A4-positive mesenchymal niches in both follicular and eccrine elements. These niches are close to myxoid cells with PRKAR1A loss, whereas the complex epithelial structures retain PRKAR1A, which suggests that the former induce non-neoplastic growth of the latter. Finally, we provide evidence for the role of mesenchymal-to-epithelial transition in the branching of the eccrine duct epithelium in the Carney's complex case, probably secondary to protein kinase A activation.</p>\u0000 </section>\u0000 </div>","PeriodicalId":13219,"journal":{"name":"Histopathology","volume":"87 2","pages":"321-328"},"PeriodicalIF":3.9,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/his.15478","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144215706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic correlates of malignancy in TFE3-rearranged PEComa: a series of 14 cases","authors":"David J Papke Jr.,&nbsp;Igor Odintsov,&nbsp;Navin R Mahadevan,&nbsp;Christopher DM Fletcher,&nbsp;John Hanna","doi":"10.1111/his.15476","DOIUrl":"10.1111/his.15476","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p>Perivascular epithelioid cell tumours (PEComas) show variable smooth muscle and melanocytic differentiation and mostly harbour mTOR pathway activation via <i>TSC2</i> inactivation. Five–10% of sporadic PEComas instead harbour fusions involving <i>TFE3</i>, an vmTOR pathway target. Malignancy in <i>TSC2</i>/<i>1</i>-inactivated PEComa correlates with <i>TP53</i>, <i>RB1</i> or <i>ATRX</i> inactivation. Here, we investigated genetic correlates of malignancy in <i>TFE3</i>-rearranged PEComa.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods and Results</h3>\u0000 \u0000 <p>Fourteen <i>TFE3</i>-rearranged PEComas, confirmed by FISH and/or sequencing, occurred in 11 females (79%) and 3 males aged 9–64 years (median: 31.5 yr). Body sites were uterus (3 tumours), extremities (3), colon (2), nasal cavity (2), neck (1), retroperitoneum (1), bladder (1) and ovary (1). Nine tumours (64%) lacking cytologic atypia were diagnosed prospectively as benign, and five cytologically atypical tumours were diagnosed prospectively as malignant.</p>\u0000 \u0000 <p>By immunohistochemistry, tumours expressed SMA (6/13; 46%), HMB-45 (5/13; 38%), desmin (3/13; 23%) and melan-A (2/13; 15%), and not MITF (10 tumours), S-100 (7), SOX10 (4), pan-K (5) or EMA (3). By DNA sequencing, all nine benign tumours lacked complex copy number alterations (CNAs) or inactivation of <i>TP53</i>, <i>ATRX</i> or <i>RB1</i>. In contrast, three of four (75%) assessable malignant tumours showed complex CNAs, and only one of five malignant PEComas (20%) harboured <i>TP53</i> inactivation. Among eight patients with follow-up (57%), all four benign PEComas neither recurred nor metastasized (median: 5.0 yr; range: 3.3–8.1 yr), while all four malignant tumours metastasized.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>We conclude that malignant <i>TFE3</i>-rearranged PEComas frequently harbour complex CNAs, which could be of diagnostic utility. Malignant <i>TFE3-</i>rearranged PEComas lacked highly recurrent alterations in <i>TP53</i>, <i>RB1</i> or <i>ATRX</i>.</p>\u0000 </section>\u0000 </div>","PeriodicalId":13219,"journal":{"name":"Histopathology","volume":"87 3","pages":"436-445"},"PeriodicalIF":4.1,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144215707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The first reported case of effective targeted therapy in extrahepatic cholangiocarcinoma harboring an epidermal growth factor receptor exon 19 deletion","authors":"Ling-Jen Hung,&nbsp;Shih-Chiang Huang,&nbsp;Chih-Wei Wang,&nbsp;Wen-Kuan Huang,&nbsp;Ching-Fu Chang","doi":"10.1111/his.15473","DOIUrl":"10.1111/his.15473","url":null,"abstract":"","PeriodicalId":13219,"journal":{"name":"Histopathology","volume":"87 2","pages":"329-332"},"PeriodicalIF":3.9,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144173600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TERT dysregulation through intrachromosomal rearrangement in a skull-based soft tissue myoepithelial carcinoma","authors":"Prokopios P Argyris,&nbsp;Kyle K VanKoevering,&nbsp;Daniel M Jones,&nbsp;Steve Oghumu,&nbsp;O Hans Iwenofu","doi":"10.1111/his.15464","DOIUrl":"10.1111/his.15464","url":null,"abstract":"","PeriodicalId":13219,"journal":{"name":"Histopathology","volume":"87 2","pages":"337-340"},"PeriodicalIF":3.9,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comment on ‘The evolving molecular characterization, histological criteria and nomenclature of adenoid ameloblastoma as a World Health Organization tumour type’","authors":"Sampurna Raha,&nbsp;Rajiv S. Desai,&nbsp;Shivani P. Bansal,&nbsp;Pankaj M. Shirsat,&nbsp;Pooja S. Prasad","doi":"10.1111/his.15475","DOIUrl":"10.1111/his.15475","url":null,"abstract":"","PeriodicalId":13219,"journal":{"name":"Histopathology","volume":"87 3","pages":"481-482"},"PeriodicalIF":4.1,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atypical spindle cell/pleomorphic lipomatous tumour: a clinicopathologic, immunohistochemical and molecular study of 55 cases, highlighting TP53 gene alterations as a genetic hallmark of atypical pleomorphic lipomatous tumour","authors":"Fleur Cordier,&nbsp;Tony G Kleijn,&nbsp;Thomas Mentzel,&nbsp;Uta Flucke,&nbsp;Joost van Gorp,&nbsp;Liesbeth Ferdinande,&nbsp;Sarah Van Belle,&nbsp;Siebe Loontiens,&nbsp;Joni Van der Meulen,&nbsp;Jo Van Dorpe,&nbsp;Arjen H G Cleven,&nbsp;David Creytens","doi":"10.1111/his.15474","DOIUrl":"10.1111/his.15474","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p>Atypical spindle cell lipomatous tumour (ASLT) and atypical pleomorphic lipomatous tumour (APLT) have been grouped together under the umbrella designation atypical spindle cell/pleomorphic lipomatous tumour (ASPLT) in the 2020 edition of the World Health Organization (WHO) Classification of Soft Tissue and Bone Tumours. They are thought to exist on a morphologic spectrum and share similar clinicopathologic and biological characteristics. The aim of this study was to further explore the genetic background of ASLTs and APLTs by employing DNA-based next-generation sequencing and immunohistochemistry, with a specific focus on the <i>TP53</i> gene.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods and results</h3>\u0000 \u0000 <p>Using DNA-based NGS and immunohistochemistry, <i>TP53</i> alterations were identified in 20 out of 21 APLT cases (95%). This is in contrast to the ASLT cases, in which no <i>TP53</i> alterations could be observed. Among APLT cases with an abnormal p53 immunohistochemical profile and successful DNA NGS testing, 92% (12 of 13 cases) harboured a <i>TP53</i> alteration.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>APLTs predominantly harbour a <i>TP53</i> alteration in contrast to ASLT cases. Our findings support the classification of APLT as a distinct (sub)entity within a spectrum that overlaps with ASLT, and it remains to be determined whether the broader term ‘ASPLT’ will hold up. Furthermore, p53 immunostaining proved to be a potentially valuable diagnostic tool, aiding pathologists in differentiating between ASLT and APLT.</p>\u0000 </section>\u0000 </div>","PeriodicalId":13219,"journal":{"name":"Histopathology","volume":"87 2","pages":"197-205"},"PeriodicalIF":3.9,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144127402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}