Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao最新文献

{"title":"[The alteration of inflammatory cytokine during acute pancreatitis].","authors":"Xiaoli Chen,&nbsp;Hao Wu,&nbsp;Xinglan Huang,&nbsp;Xiaojuan Wu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To observe the alteration of both inflammatory and anti-inflammatory cytokines during acute pancreatitis, and to investigate the effect of somatostatin on modulation of inflammatory and anti-inflammatory cytokines in experimental acute pancreatitis.</p><p><strong>Methods: </strong>SD male rats were divided into 3 groups: group 1. the normal rats as control (n = 6); group 2, the rats with acute pancreatitis induced by transabdominal injection of 5% sodium cholate sulfur (at the volume of 1.0 ml/kg) into the parcreatic duct and not given drug treatment; group 3, the rats injected with stilamin 20 micrograms/kg, intravenously, 30 minutes after the successful induction of acute pancreatitis. The animals in groups 2 and 3 were killed at 2, 6 and 24 hours after operation. The blood samples were taken for measurement of IL-1, TNF alpha, IL-6 (by Bioassay) and IL-10, TNF-beta (by ELISA). The wet weight of pancreatic tissue and amylase were also determined.</p><p><strong>Results: </strong>Serum IL-1, TNF alpha, IL-6, IL-10 and TGF-beta in control group were 0.56 +/- 0.06 ng/ml, 23.50 +/- 1.87 IU/ml, 69.0 +/- 6.40 IU/ml, 32.05 +/- 14.87 pg/ml and 66.4 +/- 13.20 pg/ml, respectively. After acute pancreatitis was induced, the serum level of these inflammation-concerned cytokines increased significantly in group 2 (P < 0.05). Serum IL-1, TNF alpha, IL-6, IL-10 and TGF-beta in group 2 at 24 hours after pancreatitis were 1.15 +/- 0.13 ng/ml, 55.33 +/- 12.79 IU/ml. 127.17 +/- 13.91 IU/ml, 68.13 +/- 19.90 pg/ml, and 103.77 +/- 28.95 pg/ml, respectively. After administration of somatostatin, the inflammation-concerned cytokines in group 3 were remarkably decreased (P < 0.05). Serum IL-1, TNF alpha, IL-6, IL-10 and TGF-beta in group 3 were 0.83 +/- 0.12 ng/ml, 33.00 +/- 7.40 IU/ml. 71.83 +/- 6.34 IU/ml, 42.2 +/- 14.55 pg/ml, and 45.98 +/- 18.10 pg/ml, respectively. The indeies of the severity of pancreatitis, such as amylase and the weight of pancreas alse improved in group 3.</p><p><strong>Conclusion: </strong>Both inflammatory and antiinflammatory cytokines increased remarkably in the rats with acute pancreatitis. This result indicates that there is a potential tendency of inflammatory response syndrome and compensatory anti-inflammatory response syndrome in the course of acute pancreatitis. Somatostatin can modulate the derangement of these cytokines in acute pancreatitis.</p>","PeriodicalId":13173,"journal":{"name":"Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao","volume":"33 2","pages":"238-40, 243"},"PeriodicalIF":0.0,"publicationDate":"2002-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22236154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Relationship between the infiltration arts and penetration depth of molten glass into aluminum oxide matrix].","authors":"Yandong Mu,&nbsp;Yonglie Chao,&nbsp;Yunmao Liao,&nbsp;Yukun Meng","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To explore the influences of infiltration time and temperature on the penetration depth of GI-II glass into alumina matrices, thus providing a theoretical basis for facilitating the clinical fabrication of all-ceramic restorations.</p><p><strong>Methods: </strong>After preparing cuboid alumina specimens 7 x 7 x 5 mm3 in size, we carried out the infiltration firing procedure at 1100 degrees C for six different time duration (30, 60, 90, 120, 150, 180 minutes), and at five different temperatures (1060, 1080, 1100, 1120, 1140 degrees C) for 1 hour each. The penetration depth of glass into the alumina matrices was measured under a stereoscope with magnification of 10 times.</p><p><strong>Results: </strong>The infiltration time duration and penetration depth were not linearly but exponentially related for both Vita and GI-II materials (r = 0.9886 for Vita and r = 0.9932 for GI-II). The regression equations were as follows: d2 = 0.1122t - 0.4955 (Vita) and d2 = 0.1638t + 0.5873 (GI-II). The temperature and depth were not linearly related either, with increased penetration speed under higher temperatures for GI-II material; the tendency for Vita material was just the opposite.</p><p><strong>Conclusion: </strong>At an infiltration temperature of 1100 degrees C, the molten infiltration time duration of GI-II glass could be reduced to 1 hour for copings and 3 hours for anterior bridge substructures due to its better infiltration ability.</p>","PeriodicalId":13173,"journal":{"name":"Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao","volume":"33 2","pages":"212-4, 237"},"PeriodicalIF":0.0,"publicationDate":"2002-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22236222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Construction of DNA vaccine including a chimeric gene encoding flagellin and outer membrane protein antigen from Leptospira interrogons serovar lai].","authors":"Min Wang,&nbsp;Baomin Dai,&nbsp;Zill You,&nbsp;Zhimao Fang,&nbsp;Yajing Wang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To construct a fusion expression vector for DNA vaccine, including a flagellin gene (flaB2) and an outer membrane protein gene (ompL1) to enhance the protective immunity of Leptospira interrogans serova Lai (Stain 017).</p><p><strong>Methods: </strong>The DNA fragments encoding flaB2 and ompL1 were amplified respectively by PCR and then fused. The obtained chimeric gene ompL1-flaB2 contained a synthetic linker of ten amino acids to sustain its space conformation.</p><p><strong>Results: </strong>Identified by restriction enzyme analysis, an insert fragment of 1.8 kb was demonstrated. FlaB2 and ompL1 respectively had the same sequence as reported by DNA sequencing analysis.</p><p><strong>Conclusion: </strong>A fusion expression plasmid containing flaB2 and ompL1 was constructed.</p>","PeriodicalId":13173,"journal":{"name":"Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao","volume":"33 2","pages":"169-71"},"PeriodicalIF":0.0,"publicationDate":"2002-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22236271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Comparison of the immune response to gene transfer into the rat brain with helper or helper virus-free HSV-1 vectors].","authors":"Wei Zhang,&nbsp;A Geller","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>This study was conducted to compare the immune response to gene transfer with helper virus or helper virus-free vectors.</p><p><strong>Methods: </strong>Two vector stocks were delivered by stereotactic injection into the striatum and then the immune response markers including TCR, CD4, CD8, MHC-I and MHC-II were examined.</p><p><strong>Results: </strong>The data showed that the immune response to either vector stock reached maximal levels by 4 to 7 days after gene transfer. However, the maximal levels of all markers to the helper virus-free stock were 3 to 10 folds lower than the markers to the helper virus stock. The immune response to the helper virus-free stock declined rapidly to be close to the levels of PBS control.</p><p><strong>Conclusion: </strong>The immune response to the helper virus-free stock is significantly less than the response to the vector stock that contains helper virus.</p>","PeriodicalId":13173,"journal":{"name":"Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao","volume":"33 2","pages":"175-8"},"PeriodicalIF":0.0,"publicationDate":"2002-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22236273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The role of progesterone in the regulation of gene expression of insulin-like growth factor-I receptor in human decidual stromal cells of early pregnancy in vitro].","authors":"Shangwei Li,&nbsp;Lei Li,&nbsp;Zeyi Cao,&nbsp;Zhilan Peng,&nbsp;Ziyan Han,&nbsp;Yezhou Yang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To assess the effect of progesterone on the gene expression of insulin-like growth factor-I receptor (IGF-I R) in human decidual stromal cells of early pregnancy in vitro.</p><p><strong>Methods: </strong>Semiquantitative reverse transcriptase polymerase chain reaction, using beta-ACTIN as internal standard, was applied to determine the levels of IGF-I R mRNA in human decidual stromal cells of early pregnancy in vitro after cultured with different concentrations of progesterone for 72 hours or cultured with 0.1 mumol/L of progesterone for different periods of time.</p><p><strong>Results: </strong>The expression of IGF-I R mRNA was significantly positive in human decidual stromal cells of early pregnancy in vitro. The levels of IGF-I R mRNA were down-regulated by progesterone, and showed significant negative-correlation with the concentration of progesterone (r = -0.680, P < 0.001). The levels of IGF-I R mRNA showed no significant correlation with time when the final concentration of progesterone was 0.1 mumol/L (r = 0.005, P > 0.05).</p><p><strong>Conclusion: </strong>Progesterone may play an important role in the regulation of proliferation and decidualization of stromal cells by down-regulating the expression of IGF-I R mRNA in human decidual stromal cells of early pregnancy, which is important for the maintenance of early pregnancy.</p>","PeriodicalId":13173,"journal":{"name":"Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao","volume":"33 2","pages":"183-5, 188"},"PeriodicalIF":0.0,"publicationDate":"2002-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22236275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Study on the relationship between cervical cancer and p53 codon 72 polymorphism].","authors":"Mingrong Qie,&nbsp;Yanhua Zhang,&nbsp;Junmei Wu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>A polymorphism at codon 72 of the human tumor-suppressor gene, p53, results in translation to either arginine or proline. Some recent reports suggested that the risk of HPV-associated cervical cancer in white women is higher for those homozygous for the arginine allele than for those who are heterozygous; some reports did not. So we examined a number of cervical cancer, ovarian cancer and normal control to see if we could confirm this result.</p><p><strong>Methods: </strong>Three groups of women were studied: 20 women for normal control; 15 women with ovarian cancer; 15 women with cervical cancer. DNA from peripheral blood samples and from pathologic tissue sections was examined by PCR with allele-specific primers.</p><p><strong>Results: </strong>The proportions of individuals homozygous for the arginine allele, homozygous for the proline allele and heterozygous for the two alleles were 38%, 6% and 56% in normal women; 38%, 5% and 57% in women with ovarian cancer; 78%, 8% and 14% in women with cervical cancer, respectively. Chi-square analysis showed significant differences in the proportions.</p><p><strong>Conclusion: </strong>In this population, individuals homozygous for the arginine variant of codon 72 of the p53 gene were at increased risk of cervical cancer.</p>","PeriodicalId":13173,"journal":{"name":"Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao","volume":"33 2","pages":"274-5"},"PeriodicalIF":0.0,"publicationDate":"2002-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22235991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The expression of collagen type I and type II during the second fracture healing].","authors":"Xian Zhang,&nbsp;Fan Wang,&nbsp;Hongmei Jiang,&nbsp;Ruixiang Li","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>This study was conducted to disclose the rules of collagen type I and II production and hence to acquire knowledge about the mechanism of repairing during the second period of fracture healing.</p><p><strong>Methods: </strong>An experimental model of second fracture healing at rat's femurs was used to study the production of collagen type I and II by means of immunohistochemistry; meanwhile the histologic changes were observed with the use of HE staining and Alcian blue staining at 3 days, 1, 2, 3, 4 weeks postoperatively.</p><p><strong>Results: </strong>The expression of both collagen type I and II was detected by immunohistochemistry at 1 week. The peak expression of collagen type II occurred at 3 weeks. The higher expression of collagen type I was observed at 4 weeks.</p><p><strong>Conclusion: </strong>The morphologic changes of chondrocyte and the production of collagen type I and II by chondrocyte in cartilage callus are correlated with their repairing function during different periods of fracture healing.</p>","PeriodicalId":13173,"journal":{"name":"Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao","volume":"33 2","pages":"204-6"},"PeriodicalIF":0.0,"publicationDate":"2002-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22236218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect of para-aminobenzoic acid on the growth of Actinomyces viscosus].","authors":"Bin Guo,&nbsp;Xuedong Zhou,&nbsp;Xiaorong Xiao,&nbsp;Lei Li,&nbsp;Zhu Zhu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To examine the effects of para-aminobenzoic acid (PABA) on the growth of Actinomyces viscosus.</p><p><strong>Methods: </strong>Different concentrations of PABA (10(-10)-10(-3) g/L) were each transferred to modified Carlsson medium. Actinomyces viscosus ATCC19246 grew in them. And the cultures were incubated at 37 degrees C anaerobically in the atmosphere of 80%N2, 10%H2, 10%CO2 for 48 h. Actinomyces viscosus OD values (lambda = 540 nm) were obtained with UV-1601. Colony forming unit (CFU) was established by growth of Actinomyces viscosus in culture when different concentrations of PABA (10(-10)-10(-3) g/L) were present.</p><p><strong>Results: </strong>Different concentrations of PABA (10(-10)-10(-4) g/L) had different stimulating effects on the growth of Actinomyces viscosus (P < 0.05). But this kind of stimulating effect declined when PABA concentration was 10(-5) g/L, and as PABA (10(-3) g/L) was present, this kind of effect was missing.</p><p><strong>Conclusion: </strong>The phenomena indicated that PABA has stimulating effect on the growth of Actinomyces viscosus, particularly when PABA is at the concentration of 10(-6) g/L.</p>","PeriodicalId":13173,"journal":{"name":"Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao","volume":"33 2","pages":"210-1, 219"},"PeriodicalIF":0.0,"publicationDate":"2002-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22236221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Discharge patterns of non-motoneurons in the facial nucleus of rats].","authors":"Hengxiu Yan,&nbsp;Chengwu Zhang,&nbsp;Yu Zheng","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To explore the discharge patterns of non-motoneurons in the facial nucleus of rats.</p><p><strong>Methods: </strong>After retrograde degeneration of the facial motoneurons induced by section of the facial nerve, the discharge of non-motoneurons in the facial nucleus was extracellularly monitored, and their phase relations to the respiratory cycle were investigated.</p><p><strong>Results: </strong>In 18 rats, totally 142 non-motoneurons with spontaneous discharge were recorded in the facial nucleus. Among them, 127 (89.4%) were non-respiratory related neurons (NRRNs) and 15 (10.6%), respiratory related neurons (RRNs).</p><p><strong>Conclusion: </strong>There exist different kinds of nonmotoneurons in the facial nucleus. The results of this study suggest that the facial nucleus not only controls the movement of the facial skeletal muscles, but also participates in the regulation of other functions such as respiration.</p>","PeriodicalId":13173,"journal":{"name":"Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao","volume":"33 2","pages":"189-91"},"PeriodicalIF":0.0,"publicationDate":"2002-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22236277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[A study on the influence factors for social adaptive behavior of children].","authors":"Xiaofei Zhao,&nbsp;Qiushui Zhang,&nbsp;Youhe Shan,&nbsp;Huikai Zhang,&nbsp;Lanting Guo","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the influence factors for social adaptive behavior of children.</p><p><strong>Methods: </strong>Six hundred children of 6 to 12 years old were investigated with the adaptive behavior assessment form of American children (AAMD-ABS revised edition). The influence factors for social adaptability of children were analyzed by stepwise regression.</p><p><strong>Results: </strong>It was found that preschool fostering type, parents' relation, social mood of living area, and mother's age and health state enforced a remarkable influence on the social adaptive behavior of children.</p><p><strong>Conclusion: </strong>A good fostering type, parents' harmonious relationship, health children's social environment and good health of mother would help improve children's social adaptability.</p>","PeriodicalId":13173,"journal":{"name":"Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao","volume":"33 2","pages":"259-61"},"PeriodicalIF":0.0,"publicationDate":"2002-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22235409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}