Idengaku zasshi最新文献_第2页

Reproductive isolation between Drosophila lini and its siblings. 果蝇及其兄弟姐妹之间的生殖隔离。

Idengaku zasshi Pub Date : 1995-06-01 DOI: 10.1266/jjg.70.311

Y Oguma, S Wen, M Tomaru, H Matsubayashi, T Peng

引用次数: 9

Nucleotide substitution type dependence of generation time effect of molecular evolution. 分子进化中核苷酸取代类型依赖性的代时间效应。

Idengaku zasshi Pub Date : 1995-06-01 DOI: 10.1266/jjg.70.373

Y Kisakibaru, H Matsuda

{"title":"Nucleotide substitution type dependence of generation time effect of molecular evolution.","authors":"Y Kisakibaru, H Matsuda","doi":"10.1266/jjg.70.373","DOIUrl":"https://doi.org/10.1266/jjg.70.373","url":null,"abstract":"Using DNA sequence data of 18 genes from 14 mammals, we analyzed how the average molecular evolution rate per year per site (Vy) depends on the generation time (g). (I) Assuming the relation Vy varies; is directly proportional to g(-alpha), the index of generation time effect, (alpha) was estimated to be about 0.14 for amino acid replacement substitutions (A), and about 0.32 for synonymous substitutions (S). (II) Assuming the relation Vy = V(m)g g-1 + V(e)y, where V(m)g and V(e)y are constant independent of g, the fraction, r(e) = V(e)y/Vy, of the mutation rate independent part (V(e)y) in the total evolution rate (Vy) was estimated under the assumptions of the star phylogeny and the constancy of the mutation rate per generation. r(e) was smallest for mouse with the shortest generation time among our analyzed species, and it was estimated to be about 0.57 for A and 0.31 for S. Both results do not support the view that Vy is equal to the neutral mutation rate per site both for A and for S. They are in line with the thesis that, at least for A and probably even for S, the molecular evolution rate is influenced by some causes other than the mutation rate, such as changing environment.","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"70 3","pages":"373-86"},"PeriodicalIF":0.0,"publicationDate":"1995-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.70.373","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18636262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Cloning and characterization of cDNAs for 70-kDa heat-shock proteins (Hsp70) from two fish species of the genus Oryzias. 两种水稻属鱼类70-kDa热休克蛋白(Hsp70) cdna的克隆与鉴定。

Idengaku zasshi Pub Date : 1995-06-01 DOI: 10.1266/jjg.70.423

A Arai, K Naruse, H Mitani, A Shima

引用次数: 45

Genetic studies on premating isolation in Drosophila simulans. I.AD. simulans line highly crossable with D. melanogaster. 拟果蝇早熟分离的遗传学研究。I.AD。拟合线与黑腹龙高度交叉。

Idengaku zasshi Pub Date : 1995-06-01 DOI: 10.1266/jjg.70.365

T Uenoyama, Y Inoue

引用次数: 12

A conditional negative selection for Arabidopsis expressing a bacterial cytosine deaminase gene. 表达细菌胞嘧啶脱氨酶基因的拟南芥条件阴性选择。

Idengaku zasshi Pub Date : 1995-06-01 DOI: 10.1266/jjg.70.409

T Kobayashi, S Hisajima, J Stougaard, H Ichikawa

{"title":"A conditional negative selection for Arabidopsis expressing a bacterial cytosine deaminase gene.","authors":"T Kobayashi, S Hisajima, J Stougaard, H Ichikawa","doi":"10.1266/jjg.70.409","DOIUrl":"https://doi.org/10.1266/jjg.70.409","url":null,"abstract":"The enzyme activity for cytosine deaminase, which converts cytosine to uracil in bacterial, is usually undetected in higher plants and animals. The enzyme also catalyzes conversion of non-toxic 5-fluorocytosine (5-FC) to 5- fluorouracil (5-FU), a toxic compound for plant growth. The gene encoding cytosine deaminase (codA) from Escherichia coli was fused to cauliflower mosaic virus (CaMV) 35S promoter (P35S), and cloned into a binary vector pLABR101. The resulting plasmid pLABR102 contained two marker genes for plants: a positive marker gene, bialaphos resistance (bar) gene driven by the promoter from nopaline synthase gene (Pnos) and a negative one, P35S-codA. The binary vector pLABR102 was transformed into Arabidopsis thaliana via Agrobacterium-mediated transformation. In transgenic progenies (T3) of the second (T2) generation heterozygous for a single T-DNA insertion, a 3:1 segregation ratio was observed on both bialaphos (resistance to sensitive) and 5-FC (sensitive to unaffected). From T2 plants homozygous for the T-DNA insert, on the other hand, no segregation was detected: all the T3 seedlings were resistant to bialaphos and sensitive to 5-FC. PCR and Northern analyses showed that the 5-FC sensitivity in transgenic descendants was caused by the integration and expression of the chimeric codA gene in the Arabidopsis genome. The results indicated that cytosine deaminase from E. coli is functional and useful for negative selection in Arabidopsis, and that sensitivity to 5-FC as well as the positive bialaphos resistance are dominant traits in Arabidopsis.","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"70 3","pages":"409-22"},"PeriodicalIF":0.0,"publicationDate":"1995-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.70.409","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18636874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Characterization of a family of tandemly repetitive DNA sequences from the fern sawfly, Strongylogaster osmundae (Hymenoptera: Tenthredinidae). 膜翅目:大叶蛾科(Strongylogaster osmundae)一个串联重复DNA序列的鉴定。

Idengaku zasshi Pub Date : 1995-04-01 DOI: 10.1266/jjg.70.167

S Sonoda, T Yamada, T Naito, F Nakasuji

引用次数: 0

P element-induced mutations in Drosophila melanogaster showing high interspecific crossability between D. melanogaster females and D. simulans males. P元素诱导的黑腹果蝇突变显示黑腹果蝇雌性与拟南果蝇雄性之间具有较高的种间杂交能力。

Idengaku zasshi Pub Date : 1995-04-01 DOI: 10.1266/jjg.70.197

Y Oguma, M Sawa, H Matsubayashi, M Nonaka, M Tomaru

{"title":"P element-induced mutations in Drosophila melanogaster showing high interspecific crossability between D. melanogaster females and D. simulans males.","authors":"Y Oguma, M Sawa, H Matsubayashi, M Nonaka, M Tomaru","doi":"10.1266/jjg.70.197","DOIUrl":"https://doi.org/10.1266/jjg.70.197","url":null,"abstract":"Homozygous stocks for the second or the third chromosome of Drosophila melanogaster with a single insertional plwB element were screened for high crossability with D. simulans. Reciprocal crosses between each of these stocks and D. simulans were made, and the insemination rate at two or three days was examined. From two cycles of screening of the original 575 stocks, one stock (# 687) which showed high insemination rate was selected and was backcrossed to a w strain to substitute the background. We obtained a stock which showed 10% insemination rate with D. simulans males (control was 0%). No stocks exhibiting a high crossability with D. simulans females were acquired. Revertant strains, from which the P element had been lost, were obtained from the backcrossed # 687 stock. The insemination rates of 13 revertants to D. simulans males ranged from 1% to 33%. Seven of these 13 were not significantly different from the control line but were significantly different from the backcrossed # 687 stock. It was concluded that the mutation showing high crossability with D. simulans males was caused by the P element transposition.","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"70 2","pages":"197-209"},"PeriodicalIF":0.0,"publicationDate":"1995-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.70.197","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18611485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Cloning, nucleotide sequence, and expression of tef-1, the gene encoding translation elongation factor 1 alpha (EF-1 alpha) of Neurospora crassa. 粗神经孢子虫翻译伸长因子1 α (EF-1 α)基因tef-1的克隆、核苷酸序列及表达

Idengaku zasshi Pub Date : 1995-04-01 DOI: 10.1266/jjg.70.273

A Ichi-ishi, H Inoue

引用次数: 13

Epistatic interactions among the P element-induced high interspecific crossability strains in Drosophila melanogaster. P元素诱导的黑腹果蝇高种间杂交株系间的上位性相互作用。

Idengaku zasshi Pub Date : 1995-04-01 DOI: 10.1266/jjg.70.211

H Matsubayashi, M Tomaru, M Sawa, M Nonaka, Y Oguma

{"title":"Epistatic interactions among the P element-induced high interspecific crossability strains in Drosophila melanogaster.","authors":"H Matsubayashi, M Tomaru, M Sawa, M Nonaka, Y Oguma","doi":"10.1266/jjg.70.211","DOIUrl":"https://doi.org/10.1266/jjg.70.211","url":null,"abstract":"Five Drosophila melanogaster strains showing high interspecific crossability with D. simulans males, derived from the previous screening of a set of autosomal plwB transposants, were selected and the effect of the plwB insertion under the same genetic background (white strain) on the interspecific crossability was examined. This phenotype was recessive in the two strains but semidominant in the other three strains. Trans-heterozygotes, however, showed high interspecific crossability compared with the parental homozygotes, suggesting some epistatic interactions between them. In three strains, the effect of the plwB insertion region in different backgrounds (w1118 strain) on the crossability was also tested. Homozygotes of a strain (#687) showed high interspecific crossability comparable to the w background, while homozygotes of both #68 and #783 strains showed lower crossability than w1118. Although #783 heterozygotes showed intermediate values between #783 homozygotes and w1118, #68 heterozygotes showed a significantly higher insemination rate than w1118 and the #68 homozygotes. These results suggest that the region around the plwB insertion sites of #68 and #783 affects the interspecific crossability either positively or negatively depending on the genetic background. In all the stocks, positive correlation between interspecific crossability and the intraspecific mating speed was detected.","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"70 2","pages":"211-21"},"PeriodicalIF":0.0,"publicationDate":"1995-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.70.211","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18611486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Molecular and genetic dissection of a reproductive isolation gene, zygotic hybrid rescue, of Drosophila melanogaster. 黑腹果蝇生殖隔离基因合子杂交拯救的分子和遗传解剖。

Idengaku zasshi Pub Date : 1995-04-01 DOI: 10.1266/jjg.70.223

K Sawamura, A Fujita, R Yokoyama, T Taira, Y H Inoue, H S Park, M T Yamamoto

引用次数: 18