Journal of Advanced Medical and Pharmaceutical Research最新文献

Comparative Neuroprotective Activities of the Ethanol Extracts of Washingtonia filifera 华盛顿仙客来乙醇提取物的神经保护活性比较

Journal of Advanced Medical and Pharmaceutical Research Pub Date : 2024-07-20 DOI: 10.21608/jampr.2024.285984.1068

Aalaa Salem, El-Sayed Marwan, Saleh H. El-Sharkawy, Amira Mira

{"title":"Comparative Neuroprotective Activities of the Ethanol Extracts of Washingtonia filifera","authors":"Aalaa Salem, El-Sayed Marwan, Saleh H. El-Sharkawy, Amira Mira","doi":"10.21608/jampr.2024.285984.1068","DOIUrl":"https://doi.org/10.21608/jampr.2024.285984.1068","url":null,"abstract":"The present study aimed to the in vitro investigation of the ethanol extracts of Washingtonia filifera roots (W.R), barks (W.B), leaves (W.L), fruitless bunches (W.F) and seeds (W.S) for their neuroprotective activities including the phenolic content, antioxidant activity, anti-inflammatory activity and the potentiality to protect mouse neuroblastoma (neuro-2A) cells against H 2 O 2 and A β 1 - 42 -induced neurotoxicity. Among the extracts, W.L and W.F had the highest phenolic content; 226.7 and 172.3 GAE, respectively. Consequently, W.L and W.F showed pronounced DPPH radical scavenging activity with IC 50 values of 19.95 and 30.46 µg/mL, respectively. The highest inhibitory potential of COX-2 was achieved by W.F then W.L with IC 50 values of 111.62 and 191.77 µg/mL. The most efficient extract for the neuroprotection against H 2 O 2 and A β 1 - 42 -induced neurotoxicity was W.F which had more potent activity than the positive control in A β 1 - 42 assay. To the best of our knowledge, this is the first report to indicate the neuroprotective activities of W. filifera leaf and the fruitless bunch extracts.","PeriodicalId":130435,"journal":{"name":"Journal of Advanced Medical and Pharmaceutical Research","volume":"117 33","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141820197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the effect of sub-inhibitory concentrations of ceftriaxone on combating multidrug-resistant Staphylococcus aureus 评价亚抑制浓度头孢曲松对抗耐多药金黄色葡萄球菌的影响

Journal of Advanced Medical and Pharmaceutical Research Pub Date : 2024-07-13 DOI: 10.21608/jampr.2024.283820.1066

Amira Omar, Tarek Elsaeed Elbanna, Fatma Sonbol, M. El-Bouseary

引用次数: 0

Isocratic RP-HPLC Method for detection of Sildenafil in some Marketed Dietary Supplements 等度 RP-HPLC 法检测某些市售膳食补充剂中的西地那非

Journal of Advanced Medical and Pharmaceutical Research Pub Date : 2024-07-06 DOI: 10.21608/jampr.2024.284952.1067

Amira H. Kamal, M. Mabrouk, Sherin F Hammad, Badiea Soliman

{"title":"Isocratic RP-HPLC Method for detection of Sildenafil in some Marketed Dietary Supplements","authors":"Amira H. Kamal, M. Mabrouk, Sherin F Hammad, Badiea Soliman","doi":"10.21608/jampr.2024.284952.1067","DOIUrl":"https://doi.org/10.21608/jampr.2024.284952.1067","url":null,"abstract":"Due to the widespread use of sildenafil, the market is full of different counterfeited preparations that contain either an unknown amount of sildenafil or mixed with one or more different varieties of unknown substances. These fake preparations are usually harmful to users. So, it is always necessary to develop specific as well as sensitive analytical procedures to detect sildenafil in such preparations. HPLC that equipped with modern sensitive detectors such as diode array detector (DAD) can play a great role in solving such problems. In this work, a new HPLC method was established for the detection of sildenafil in some marketed dietary supplements. Thermo scientific ODS HYPERSIL column (250 x 4.6 mm, 5µm) was used. The mobile phase was composed of methanol and potassium di-hydrogen phosphate buffer 0.05M (pH 3.5) at a ratio of 70:30 (v/v). Triethylamine (0.3%) was added to the mobile phase. The flow rate was 1mL/min. Detection was performed at 290 nm using a diode array detector. The method showed good linearity (5.0-80.0 µg/mL). The retention time was 5.3 min. The validation parameters were evaluated as per ICH guidelines. The findings given by the developed method were compared statistically to those achieved by a reported one. There was a good statistical agreement. The developed method is considered a sensitive and specific analytical method for the detection of sildenafil counterfeited dietary supplements.","PeriodicalId":130435,"journal":{"name":"Journal of Advanced Medical and Pharmaceutical Research","volume":" 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141672880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of formulation variables on drug release from bilosomes; effect of cholesterol concentration 配方变量对双糖体药物释放的影响；胆固醇浓度的影响

Journal of Advanced Medical and Pharmaceutical Research Pub Date : 2024-06-01 DOI: 10.21608/jampr.2024.290747.1073

T. Elebyary, Amal Sultan, Sally El-Sayed Abu-Risha, G. E. El Maghraby

{"title":"Effect of formulation variables on drug release from bilosomes; effect of cholesterol concentration","authors":"T. Elebyary, Amal Sultan, Sally El-Sayed Abu-Risha, G. E. El Maghraby","doi":"10.21608/jampr.2024.290747.1073","DOIUrl":"https://doi.org/10.21608/jampr.2024.290747.1073","url":null,"abstract":"Bilosomes are bile salts-containing vesicles that have recently drawn attention as a novel nanocarrier for drugs. Compared to traditional nanocarriers, bilosomes have the advantage of being able to withstand disruption caused by physiological bile salts normally secreted in the gastrointestinal tract. In addition to nonionic surfactants and bile salts, cholesterol is one important ingredient in bilosomes composition. This work investigated the effect of cholesterol concentration on the release rate of the entrapped drug. Tamoxifen was used as a model drug that suffers from poor oral bioavailability due to poor solubility and extensive pre-systemic degradation. Bilosomes composed of Span 60, cholesterol, and bile salts were prepared. Cholesterol was used at two different concentrations of 0.4% and 0.8% w/v, producing formulations BiL1 and BiL2, respectively. The entrapment efficiency and in vitro drug release were evaluated using Franz diffusion cells. Increasing cholesterol concentration reduced drug release. The release efficiency values after 24 hours of release study were 9.7 and 6.8% for BiL1 and BiL2, respectively. This indicates that increasing cholesterol concentration increased the rigidity of the bilosomal membrane and enhanced drug encapsulation. Reduced release would indicate that the vesicles retain the encapsulated drug, which is advantageous, taken into consideration the lymphatic absorption of the vesicles.","PeriodicalId":130435,"journal":{"name":"Journal of Advanced Medical and Pharmaceutical Research","volume":"67 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141276871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ERCC1 rs11615 Single Nucleotide Polymorphism in Bladder Cancer Patients and Safety Outcomes of Cisplatin-Based Chemotherapy 膀胱癌患者中的 ERCC1 rs11615 单核苷酸多态性与顺铂化疗的安全性结果

Journal of Advanced Medical and Pharmaceutical Research Pub Date : 2024-03-01 DOI: 10.21608/jampr.2024.271205.1063

Khaled Kotb, Moetaza Soliman, Amira Awadallah, Asmaa Ahmed, Hassan Abol-Enein, Mohamed Shams

{"title":"ERCC1 rs11615 Single Nucleotide Polymorphism in Bladder Cancer Patients and Safety Outcomes of Cisplatin-Based Chemotherapy","authors":"Khaled Kotb, Moetaza Soliman, Amira Awadallah, Asmaa Ahmed, Hassan Abol-Enein, Mohamed Shams","doi":"10.21608/jampr.2024.271205.1063","DOIUrl":"https://doi.org/10.21608/jampr.2024.271205.1063","url":null,"abstract":"Objectives To investigate the relationship between single nucleotide polymorphism (SNP) of excision repair cross-complementation group 1 (ERCC1) rs11615 and the safety outcomes of cisplatin-based adjuvant chemotherapy in post-cystectomy bladder cancer patients. Methods This pilot single-center retrospective cohort research study involved 25 patients with muscle-invasive bladder cancer who were admitted to the Urology and Nephrology Center (UNC), Mansoura University, Egypt between January and December 2016. The study tried to reveal the association between SNP rs11615 in ERCC1 gene and the change from baseline characteristics in a median of 96 weeks follow-up in blood, renal, and hepatic biochemical parameters. Results Univariate analysis of the association between rs11615 and post-chemotherapy changes in uric acid and hemoglobin levels showed a statistically significant relationship. Conclusion These findings hint at possible genetic influences in response to chemotherapy, necessitating further research to understand the mechanisms and potential clinical implications. This could lead to personalized treatment plans enhancing patient care and outcomes.","PeriodicalId":130435,"journal":{"name":"Journal of Advanced Medical and Pharmaceutical Research","volume":"78 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140403171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bio-Modulation of Post-Harvested Potato: LC-ESI-MS, GC-MS Analysis, Antioxidant and -amylase Activity of Treated Potato Peels 收获后马铃薯的生物调节：经处理的马铃薯皮的 LC-ESI-MS、GC-MS 分析、抗氧化剂和  淀粉酶活性

Journal of Advanced Medical and Pharmaceutical Research Pub Date : 2024-02-01 DOI: 10.21608/jampr.2024.243076.1060

Mariam Hewala, Sara Abouzeid, Yhiya Amen, Farid Badria

引用次数: 0

Bio-Modulation of Post-Harvested Potato: LC-ESI-MS, GC-MS Analysis, Antioxidant and -amylase Activity of Treated Potato Peels 收获后马铃薯的生物调节：经处理的马铃薯皮的 LC-ESI-MS、GC-MS 分析、抗氧化剂和  淀粉酶活性

Journal of Advanced Medical and Pharmaceutical Research Pub Date : 2024-02-01 DOI: 10.21608/jampr.2024.243076.1060

Mariam Hewala, Sara Abouzeid, Yhiya Amen, Farid Badria

引用次数: 0

Anti-SARS-CoV-2 and Antioxidant Activities of The Methanolic Extract and Its Fractions of Marrubium alysson L. Aerial Parts 凡夫草地上部位甲醇提取物及其组分抗sars - cov -2及抗氧化活性研究

Journal of Advanced Medical and Pharmaceutical Research Pub Date : 2023-07-28 DOI: 10.21608/jampr.2023.212799.1056

Eman Ezz

{"title":"Anti-SARS-CoV-2 and Antioxidant Activities of The Methanolic Extract and Its Fractions of Marrubium alysson L. Aerial Parts","authors":"Eman Ezz","doi":"10.21608/jampr.2023.212799.1056","DOIUrl":"https://doi.org/10.21608/jampr.2023.212799.1056","url":null,"abstract":"The pandemic novel coronavirus disease 2019 (COVID-19) has resulted in enormous economic damage and an exclusive health crisis, so developing a cure for this respiratory infectious disease has proceeded forward at full speed. Natural products and herbal medicines with accepted safety profiles are considered promising sources for discovering new medication leads. Genus Marrubium was reported to exhibit potential antiviral and antioxidant activities. Thus, this study aims to evaluate the inhibitory activity of the total methanolic extract and its soluble fractions: (petroleum ether, methylene chloride, and ethyl acetate) of the aerial parts of Marrubium alysson L . against SARS-CoV-2 main protease (M pro ). Also, they were quantitatively assayed for their phenolics and flavonoids contents, in addition to evaluating their antioxidant activity. The results showed that the petroleum ether fraction has the highest inhibitory activity against SARS-CoV-2 main protease (M pro ) enzyme with IC 50 value of 3.959±0.17 µg/mL compared to the reference tipranavir (IC 50 1.89±0.08 µg/mL). The ethyl acetate fraction showed the highest total phenolics and flavonoids contents as well as the highest antioxidant activity (IC 50 33.81±0.15 µg/mL) compared to the reference L-ascorbic acid (IC 50 30.43±0.14 µg/mL). It could be concluded that soluble fractions of the total methanolic extract of Marrubium alysson L . may be used as an alternative source for developing new natural inhibitors against the main protease enzyme and may be suggested for the treatment of COVID-19.","PeriodicalId":130435,"journal":{"name":"Journal of Advanced Medical and Pharmaceutical Research","volume":"21 21","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121003928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytochemical profile, Ethnobotanical and Biological Impacts of Various Zamia Species: A Mini-Review 几种扎米亚植物化学特征、民族植物学和生物学影响综述

Journal of Advanced Medical and Pharmaceutical Research Pub Date : 2023-06-23 DOI: 10.21608/jampr.2023.202326.1053

Hosam M El-Seadawy, Kamilia A Abo El-Seoud, Mona El-Aasr, Amany E. Ragab

引用次数: 0

Colorimetric Sensor for Determination of Pregabalin Based on Localized Surface Plasmon Resonance of Silver Nanoparticles 基于银纳米粒子局域表面等离子体共振的比色传感器测定普瑞巴林

Journal of Advanced Medical and Pharmaceutical Research Pub Date : 2023-05-26 DOI: 10.21608/jampr.2023.209199.1055

M. Abdel Hamid, Ahmed A. Habib, M. Mabrouk, Sherin F Hammad, M. Elshahawy

引用次数: 0