Human reproduction最新文献

{"title":"P-735 Regional Trends in Licensing and Certification of Clinical Embryologists: Insights from a Cross-Sectional Analysis","authors":"H Burjaq, G Raad, N Younis, A Alhourani, A Almohammadi, F Choucair","doi":"10.1093/humrep/deaf097.1040","DOIUrl":"https://doi.org/10.1093/humrep/deaf097.1040","url":null,"abstract":"Study question Does national licensing influence the likelihood of clinical embryologists obtaining ESHRE certification? Summary answer Licensing for embryologists in Europe is associated with a higher likelihood of pursuing ESHRE certification, a pattern not observed in regions such as Arab countries. What is known already The demand for clinical embryologists has increased due to the growing complexity of IVF cycles and staffing requirements. However, professionalism varies significantly across countries, with differences in training, recognition, and regulatory frameworks. Few studies have examined workforce regulations, with only one mapping European nations, revealing limited mandatory licensing. Since its introduction in 2008, ESHRE certification—enhanced by a proctored exam since 2021—has become the first standardized credential. Given these disparities, it is essential to assess whether national regulations influence clinical embryologists' pursuit of ESHRE certification as a means of validating expertise and enhancing professional recognition. Study design, size, duration This cross-sectional, cross-regional study examines the relationship between national licensing regulations and ESHRE certification among clinical embryologists in European and Arab countries. Primary data is collected through a survey of key informants across 17 Arab countries to assess licensing status. Secondary data includes literature-based licensing information for European countries. Both datasets are cross-matched with the number of ESHRE-certified embryologists listed on the ESHRE website up to 2024, providing a comprehensive regional comparison. Participants/materials, setting, methods Purposeful selection of key informants was employed to ensure expert sampling across 17 Arab member states. The questionnaire covered various aspects of national regulations governing clinical embryologists. For European countries, national regulatory data was extracted from the PubMed database. The number of ESHRE-certified embryologists was obtained from the ESHRE website, including those certified up to the 2024 cycle. Associations between certification and regulatory frameworks were examined within each region. Main results and the role of chance Among the 17 Arab countries analyzed, 7 have explicit national licensing frameworks for embryologists. A total of 64 ESHRE-certified embryologists were identified in this region. No statistically significant difference was found in the number of ESHRE-certified embryologists between licensed (38) and non-licensed countries (26) (Mann-Whitney U test, p = 0.959). However, licensed Arab countries had a slightly higher mean number of certified embryologists compared to non-licensed countries. Conversely, findings from 12 European countries showed an opposite trend. Among these, eight countries have established national licensing frameworks for embryologists, with a total of 753 ESHRE-certified embryologists in licensed countries. A stat","PeriodicalId":13003,"journal":{"name":"Human reproduction","volume":"1 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144513167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"P-639 Effect of preconceptional weight optimization on metabolic parameters and in vitro fertilization outcomes in infertile women with obesity","authors":"J Zadori, N Keller, V Vedelek, D Szollosi, S Lada, A Nemes, P Kempler, A Menyhart, I Baczko, T Varkonyi, C Lengyel, A Vagvolgyi","doi":"10.1093/humrep/deaf097.945","DOIUrl":"https://doi.org/10.1093/humrep/deaf097.945","url":null,"abstract":"Study question Does preconceptional weight loss improve metabolic parameters and IVF success rates in obese, infertile women? Summary answer Weight loss before IVF significantly improved metabolic markers, and 62.5% of those who reached target weight conceived through IVF, while 18.75% conceived spontaneously. What is known already The global rise in obesity significantly impacts female reproductive health. In Hungary, 22.7% of adult women are obese. Obesity contributes to metabolic and hormonal imbalances, negatively affecting fertility. Studies indicate that excess weight impairs in vitro fertilization (IVF) outcomes, although the benefits of weight loss remain debated. Research suggests that preconceptional weight reduction improves pregnancy rates, live birth rates, and reduces the number of IVF cycles needed. Lifestyle modification is the first-line treatment for obese, infertile women, particularly those with polycystic ovarian syndrome (PCOS). Exercise alone may also enhance fertility outcomes, as evidenced by recent clinical trials. Study design, size, duration A retrospective, single-center cohort study was conducted on infertile women with obesity (BMI>30 kg/m2) the Endocrinology Outpatient Clinic, Department of Medicine in cooperation with the Institute of Reproductive Medicine at the University of Szeged. Data collection was performed from January 1, 2020, to March 31, 2024 among women undergoing weight optimization before IVF treatment. There were no exclusion criteria for participation in the study. Participants/materials, setting, methods Comparisons of baseline clinical, laboratory, and biometric parameters of the infertile women with obesity (n = 58) were made with an age-matched control group (n = 45) with normal BMI, and within the group of infertile women (n = 16) both before and after the weight loss period, which included lifestyle modifications, dietary interventions, and liraglutide. The treatment of obesity, including pharmacological treatment, was carried out according to recent Hungarian guidelines. Primary outcomes were metabolic improvements and clinical pregnancy rates. Main results and the role of chance Infertile women with obesity (n = 58; age: 33.1 ± 5.42 years; BMI: 39.3 ± 6.90 kg/m²) had significantly higher systolic (136.8 ± 14.55 vs. 113.4 ± 13.87 mmHg; p < 0.001) and diastolic blood pressure (84.7 ± 11.98 vs. 69.4 ± 11.48 mmHg; p < 0.001) compared to controls (n = 45; age: 32.1 ± 7.67 years; BMI: 21.1 ± 2.02 kg/m²). They also exhibited a higher prevalence of metabolic disorders, including hypertension (0 vs. 17; p < 0.001), impaired glucose tolerance (0 vs. 16; p < 0.001), type 2 diabetes (0 vs. 5; p < 0.05), PCOS (5 vs. 32; p < 0.001), and hypothyroidism (0 vs. 17; p < 0.001). Laboratory tests revealed significant differences in glucose metabolism, lipid profile, liver function, and inflammatory markers. Following weight loss therapy with lifestyle and","PeriodicalId":13003,"journal":{"name":"Human reproduction","volume":"10 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144513181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"P-343 Progesterone resistance related Renin-angiotensin system activation is involved in endometriosis","authors":"J Li, J Chen, D Zhang","doi":"10.1093/humrep/deaf097.650","DOIUrl":"https://doi.org/10.1093/humrep/deaf097.650","url":null,"abstract":"Study question Whether renin-angiotensin system activation could be involved in endometriosis development and act as the key linking hypertension with endometriosis. Summary answer We proved that progesterone resistance related AGT-HEY1-GATA4 regulatory axis is involved in endometriosis. Intraperitoneal injection of AGT may promote the growth of peritoneal endometriosis lesions. What is known already Patients with endometriosis may be more susceptible to hypertension, and there is currently no research exploring the molecular mechanisms underlying the association between endometriosis and hypertension. Previous studies have shown AGT’s important role in hypertension, however, it is still unknown whether the activation of the AGT pathway is involved in the occurrence and development of endometriosis, whether it is a key molecular mechanism linking endometriosis with the risk of hypertension, and whether it can serve as a potential intervention target for the progression of endometriosis. Study design, size, duration Cross-analysis of the differently expressed genes by RNA-sequencing (n = 4) and potential target genes for ERa and PR by Cut-tag (n = 1) in paired eutopic and ectopic endometrium was conducted to explore the dysregulated targets in endometriosis. Dysregulation of several AGT related markers were further confirmed by IHC, q-PCR or Western blot on tissue microarrays (n = 30) or frozen tissue (n = 10). Serum levels of AGT, MME and Progesterone were measured by ELISA in endometriosis patients and controls (n = 28). Participants/materials, setting, methods 50 endometriosis patients identified by laparoscopy and 50 controls were enrolled. A human endometrial cell line (THESC) was used as in vitro model and an endometriosis mouse model were established by the peritoneal suture and fixation of endometrium. Each experiment included at least three independent samples and was repeated at least three times. Comparisons of the two groups were made by Student’s t-tests. Differences among multiple groups were compared using a one-way analysis of variance. Main results and the role of chance AGT was found upregulated in ectopic endometrium, which was predicted to be one of the up regulators for differentially expressed genes based on IPA analysis. As AGT pathway molecules, dysregulation of AGT, AGTR1, MME, CPA3, CTSG, REN and so on were confirmed between eu-and ectopic endometrial tissues by qPCR or western blot. The cross analysis showed most of the genes differentially expressed in endometriosis lesions were regulated by ERa or PR. AGT-HEY1-GATA4 regulatory axis, which carried PR binding sites, was predicted to play a role in the development of endometriosis by IPA analysis. In vivo, we further confirmed upregulation of AGT and GATA4, downregulation of HEY1 in endometriosis lesions based on the IHC analysis of tissue microarrays of eutopic endometrium and ectopic lesions, as well as the q-PCR and western blot analysis of fresh frozen tissues. In vit","PeriodicalId":13003,"journal":{"name":"Human reproduction","volume":"17 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144503365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"P-519 Assisted Reproductive Technology (ART) with or without gamete donation: comparison between patients’ emotions and need for psychological support","authors":"R Spoletini, R De Luca, G Scaravelli, M Di Trani, A Renzi, M Mazzola, V Vigiliano, S Bolli, L Speziale, A Bertini, F Fedele","doi":"10.1093/humrep/deaf097.825","DOIUrl":"https://doi.org/10.1093/humrep/deaf097.825","url":null,"abstract":"Study question What is the need for psychological support and what are the main emotions felt by patients undergoing ART with own or donated gametes? Summary answer ART treatment with gamete donation results more emotionally charged for patients. It would be helpful for them to receive adequate psychological support during the treatment. What is known already Couples facing infertility often turn to Assisted Reproductive Technology. This may cause psychological distress due to treatment uncertainty, physical strain, and financial pressures. In Italy, ART cycles with gamete donation have risen significantly, from 2,287 (3.2% of ART activities) in 2015 to 14,543 (15.2%) in 2022. Many studies report the complexity of feelings involved in gamete donation. While it offers hope for parenthood, couples may experience mixed emotions, including sadness of losing biological ties, doubt about disclosure of the child’s origins, ambivalent attitudes towards the donor. This emotional complexity highlights the need for targeted psychological support. Study design, size, duration Between 2021 and 2023 the Italian ART Registry (IARTR), in collaboration with the Department of Dynamic and Clinical Psychology and Health Studies (Sapienza University) proposed two online questionnaires created ad hoc for the survey in two different time frames to patients underwent ART donor and non-donor treatments. The study aimed to compare the emotional sphere and needs of the patients underwent ART treatment with own or donated gametes. Participants/materials, setting, methods A total of 225 patients undergoing ART with own gametes (“non-donor patients”) and 186 patient undergoing ART with gametes donation (“donor patients”) completed the questionnaire. The groups were compared on socio-demographic characteristics, availability and type of psychological counseling offered within ART centers, and emotional responses to ART cycle experience. The primary outcome was the patients' emotional sphere related to ART treatments. Statistical analysis was conducted using Student’s t-test and Chi-square test, where appropriate. Main results and the role of chance Donor patients are significantly older (41.5 years) compared to non-donor patients (37.4 years), have been trying to conceive for a longer time (>4 years vs. <3 years), and have undergone nearly one fewer ART cycle (1.6 vs. 2.4). Donor couples discuss their infertility condition less frequently than non-donor couples, both with friends (16.7% vs. 64.4%) and with colleagues (3.8% vs. 23.1%). The presence of a psychologist at the center (88.7% vs. 56.4%) and the offer of psychological counseling (65.6% vs. 29.8%) were communicated more often to donor patients. Consequently, donor patients received psychological counseling at the ART center more frequently than non-donor patients (53.8% vs. 34.7%). Donor couples found psychological support particularly helpful during the evaluation of treatment options and during pregnanc","PeriodicalId":13003,"journal":{"name":"Human reproduction","volume":"2 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144503444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"P-424 Uterine contraction during implantation period; experience of 9,999 patients with 3 or more failure of embryo transfers","authors":"H Matsubayashi, Y Ohara, M Doshida, T Takeuchi, K Doi, K Yamaguchi, A Karino, S Saito, Y Ishibashi, K Abe, T Ishikawa","doi":"10.1093/humrep/deaf097.730","DOIUrl":"https://doi.org/10.1093/humrep/deaf097.730","url":null,"abstract":"Study question Is uterine contraction frequently observed in patients with recurrent implantation failure, and how is the frequency, direction, intensity and location? Summary answer Uterine contraction was frequently (41.3%) observed in patients with recurrent implantation failure mostly in the whole uterine cavity with “lower→upper→lower” direction. What is known already Uterine peristalsis caused by uterine contraction is thought to be one of the risk factors for implantation failure, because the uterus should be quiescent at the time of implantation period.Previous studies suggested more than 2 or 3 waves/min may be a threshold for implantation failure. Although those reports focused on frequency and direction of the uterine contraction, there were no reports regarding intensity and location of the uterine contraction. Therefore, we investigated intensity and location as well as frequency and direction of the uterine contraction in the largest number of patients with recurrent failure of embryo transfers. Study design, size, duration Transvaginal ultrasonography scans of uterine peristalsis were performed at the mid luteal phase in 9,999 patients with 3 or more failure of embryo transfers in two clinics between 2013 and 2024. The transvaginal probe (6 to 10 MHz) was introduced into the vagina as gently as possible to avoid stimulating the uterine cervix. After scanning mid-sagittal plane of the uterus, the probe was fixed as steady as possible while 3 min, video was recorded simultaneously. Participants/materials, setting, methods The video images were analyzed at 10 time the normal speed using Quick Time Player by a single observer. Frequency, intensity, location and direction of the uterine contractile activity were recorded and evaluated. Intensity was divided into 3 categories; movement with the whole endometrium (strong), with the middle and the surface of the endometrium (medium), and just the surface of the endometrium (weak). Direction was complicated with many patterns (e.g., lower→upper→lower). Main results and the role of chance Of 9,999 patients (average age, 37.4), 5,866 (58.7%) did not show any uterine contraction, 4,133 (41.3%) had uterine contraction. In the contraction group, frequency was 59.1% for 1 to 3 (times/3 min), 28.4% for 4 to 6, 10.1% for 7 to 9, and 2.4% for 10 or more. Intensity was almost equal among 3 categories (strong 25.1%, medium 41.6%, weak 33.3%). Most uterine contraction was observed in the whole uterine cavity (90.2%), whereas those in the upper, middle and lower part of the uterus were 5.0%, 0.7% and 4.1%, respectively. In terms of direction, most of uterine contraction was observed as “lower→upper→lower” (70.2%), followed by “upper→lower→upper” (9.9%), “upper→lower” (8.9%), “lower→upper” (8.4%), and unfocused (2.6%). Pregnancy outcome of patients (N = 36) who had strong uterine contraction with 10 or more was retrospectively evaluated after taking piperidolate hydrochloride (150 mg/day). Patients with ","PeriodicalId":13003,"journal":{"name":"Human reproduction","volume":"22 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144503507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"O-177 Fertility literacy in action: design and pilot results from the facts! Serious game in Europe","authors":"M Martins","doi":"10.1093/humrep/deaf097.177","DOIUrl":"https://doi.org/10.1093/humrep/deaf097.177","url":null,"abstract":"With increasing infertility rates and the global trend toward delayed parenthood, fertility education is becoming an essential tool to support informed reproductive decision-making. Adolescence represents a critical period for promoting fertility awareness and fostering healthier reproductive trajectories over the life course. To improve fertility literacy among young people, Fertility Europe and associated researchers developed Facts!—an online serious game (https://myfacts.eu/) designed for adolescents. The game features nine questions focused on fertility-related topics, including the probability of conceiving, the definition of infertility, and modifiable risk factors such as age, smoking, alcohol consumption, drug use, and physical activity. The game’s interactive format allowed participants to accumulate stars for correct answers while progressing through four virtual scenarios. In collaboration with national patient associations affiliated with Fertility Europe, the game was promoted through social media and in-school campaigns, including direct outreach in secondary schools. Data were collected between July 2023 and November 2024 in Bulgaria, Norway, Poland, and Portugal. Adolescents aged 15 to 18 were invited to participate. Of the 4,648 who completed the game, 3,555 were eligible for analysis. This pilot study analyzed differences in fertility knowledge by country, gender, and age, as well as predictors of awareness in three core domains: infertility diagnosis, probability of conception, and age-related fertility decline. The narratives of adolescents, parents and teachers about the utility of this tool and its educational potential were also addressed using focus groups. Serious games like Facts! offer a promising, scalable approach to reproductive health education, delivering evidence-based content in a format that resonates with young audiences. Preliminary findings demonstrate that Facts! effectively engages adolescents and improves their understanding of fertility. Participants, parents, and teachers responded positively, highlighting the tool’s accessibility, educational value, and ability to spark meaningful discussions on reproductive health in both formal and informal settings. These findings support the integration of fertility awareness into broader health and education strategies, with the goal of equipping adolescents with the knowledge they need to make informed decisions about their reproductive futures.","PeriodicalId":13003,"journal":{"name":"Human reproduction","volume":"643 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144503579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"O-011 A live imaging system to study the spatiotemporal control of ovulation","authors":"T L Marx, C Thomas, S M Penir, M Schuh","doi":"10.1093/humrep/deaf097.011","DOIUrl":"https://doi.org/10.1093/humrep/deaf097.011","url":null,"abstract":"Study question What can we learn about the spatiotemporal and cellular dynamics of ovulation by performing high resolution live microscopy of the entire process? Summary answer We identified three distinct, highly synchronous phases of ovulation - expansion, contraction, and rupture - and investigated their regulatory mechanisms. What is known already There have been studies performing lower-resolution live imaging, but not confocal or two-photon microscopy, of some parts of ovulation. Most of what we knew comes from fixed and knockout studies, which have shed light on key pathways such as Endothelin signaling and Hyaluronic acid. These studies identified molecular regulators of follicle rupture but lacked temporal resolution to capture dynamic processes. Some in vivo imaging studies provided insights into vascular changes and oocyte release, but real-time tracking of follicle expansion, contraction, and rupture remained unexplored. Our study bridges this gap by offering a high-resolution, continuous view of ovulation dynamics. Study design, size, duration We conducted a live-imaging study using isolated mouse ovarian follicles to analyze ovulation dynamics. The study included over 100 follicles observed over 16-24 hours to capture the full sequence of ovulation. High-resolution confocal and two photon imaging allowed us to describe cellular dynamics, observe oocyte maturation inside the intact follicle and quantify the volume of the follicles over time. We additionally generated a single-cell RNA sequencing dataset of ovulating follicles to identify new pathways. Participants/materials, setting, methods Female C57BL/6J, CAG-TAG, and Oct4-GFP mice (23-28 days old) were used. Ovarian antral follicles (300-500 µm) were isolated and cultured in optimized media under controlled conditions. Live imaging of ovulation was performed using confocal and two-photon microscopy, capturing follicle expansion, contraction, and rupture. Pharmacological perturbations were applied to investigate regulatory mechanisms. Follicle volume changes were quantified using image segmentation and 3D reconstruction. Main results and the role of chance We established a live imaging system to study ovulation in isolated mouse follicles, revealing three distinct and highly synchronous phases: follicle expansion, contraction, and rupture. Expansion is driven by hyaluronic acid secretion, creating an osmotic gradient that induces fluid influx and follicle swelling. Contraction follows, initiated by smooth muscle cells surrounding the follicle, generating hydrostatic pressure. Once a threshold is exceeded, the follicle wall rapidly stretches and ruptures, releasing the oocyte and cumulus complex. This ex vivo system faithfully recapitulates ovulatory dynamics, validated through single-cell RNA sequencing and immunofluorescence analyses. Notably, ovulatory timing was consistent across follicles, independent of size, indicating an intrinsic regulatory mechanism. The role of protease ","PeriodicalId":13003,"journal":{"name":"Human reproduction","volume":"2 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144503581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"P-777 Perinatal and neonatal outcomes, along with a long-term follow-up of children, following Capacitation In-vitro Maturation (CAPA-IVM) in women with predicted excessive ovarian response","authors":"L N Vuong, T D Pham, V N Ho, H L Le, A H Le, H H Pham, C T Tran, A N Ha, T K Le, T T Le, X T Le, M H Nguyen, B G Huynh, T M Ho","doi":"10.1093/humrep/deaf097.1081","DOIUrl":"https://doi.org/10.1093/humrep/deaf097.1081","url":null,"abstract":"Study question What are the perinatal, neonatal outcomes and long-term development of children following CAPA-IVM in women with predicted excessive response to ovarian stimulation? Summary answer Cumulative live birth rate following CAPA-IVM was 37.8%. Perinatal, neonatal and long-term developmental outcomes of children born after CAPA-IVM were within the expected normal range. What is known already CAPA-IVM may be considered a viable alternative to In-vitro Fertilization (IVF) in women with predicted excessive ovarian stimulation. Results from a randomized controlled trial comparing CAPA-IVM and IVF did not demonstrate the inferiority of CAPA-IVM following the first transfer. Existing evidence on perinatal and neonatal outcomes following CAPA-IVM indicated that CAPA-IVM yields outcomes comparable to those of IVF. However, there is a paucity of data regarding the cumulative live birth rate and long-term follow-up of children born from CAPA-IVM. Study design, size, duration We have documented the perinatal and neonatal outcomes of 1,563 CAPA-IVM cycles performed at a tertiary IVF centerfrom January 2016 to December 2023. Additionally, a long-term follow-up study was conducted on children born from a randomized controlled trial (RCT) comparing CAPA-IVM and IVF, initiated in 2018. The follow-up assessments were carried out when the children were at the ages of 6 months, 12 months, 24 months, and 60 months. Participants/materials, setting, methods Cumulative live birth rate was calculated after utilizing all embryos generated from a single CAPA-IVM cycle. Low birth weight was defined as less than 2,500 grams, while high birth weight was defined as greater than 4,000 grams. Congenital anomalies was reported according to the ICD-10 system. Children were followed up to 60 months of age and assessed for weight, height, BMI, and psychomotor development using the Ages and Stages Questionnaires (ASQ-3) and Red Flag signs. Main results and the role of chance The analysis encompassed a total of 1,563 CAPA-IVM cycles, which included 2,016 embryo transfer cycles, culminating in the birth of 658 children. Among these children, 432 were singletons and 226 were twins. The cumulative live birth rate was 37.8%. The rates of hypertension, gestational diabetes mellitus, and preterm birth were 3.5%, 15%, and 24%, respectively. Mean birth weight for singletons was 3,084.1±620.7g, while for twins was 2,458.9±679.8g. The low birth weight rate was 15%, and high birth weight was 2.5%. Congenital anomalies were observed in 1.5% of the children. In a RCT comparing CAPA-IVM and IVF, 94 children born from CAPA-IVM were compared with 104 children born from IVF at 6 months, 12 months, and 24 months of age. The two groups were comparable in terms of ASQ-3 scores. At 60 months, 36 children born after CAPA-IVM were compared with 52 children born after IVF. There were no significant differences in weightand BMI between two groups, but children born from CAPA-IVM was significantly sho","PeriodicalId":13003,"journal":{"name":"Human reproduction","volume":"26 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144503626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"P-300 Aging impacts human oocyte DNA repair response, metabolic activity and sperm DNA damage: elucidating gamete quality interlink on reproductive outcomes","authors":"F Horta, H Newman, S Mercer, M Di Battista, J Mullen, B Vollenhoven, S Catt, R Gilchrist","doi":"10.1093/humrep/deaf097.608","DOIUrl":"https://doi.org/10.1093/humrep/deaf097.608","url":null,"abstract":"Study question Are gamete biomarkers such as oocyte DNA repair response, oocyte metabolic activity and sperm DNA damage linked to reproductive aging and ART outcomes? Summary answer Human oocytes showed an altered DNA damage response and metabolic activity with aging, while increased sperm DNA damage was associated with aging. What is known already Advancing maternal age significantly impacts fertility, reproductive success, offspring health, and is closely associated with a decline in oocyte quality. Although the determinants remain elusive, DNA damage repair capacity and metabolic activity are emerging as crucial factors underpinning the age-associated deterioration in oocyte quality. The essential metabolic cofactors nicotinamide adenine dinucleotide phosphate, [NAD(P)H] and flavin adenine dinucleotide (FAD) have been negatively associated with aging in somatic tissue and immature mouse oocytes. Metabolism has been linked to expression, activation or inhibition of DNA repair proteins, which is key to repair DNA damage from both oocytes and spermatozoa. Study design, size, duration A 1-year prospective cohort study was conducted. Oocytes (n = 63) at the GV (germinal vesicle) and MI (metaphase I) stage from ART cycles (n = 32) underwent assessment for oocyte DNA repair/response biomarkers such as phosphorylated ATM (pATM), YH2AX and non-invasive metabolic activity cofactors NAD(P)H and FAD. Testing for sperm DNA damage was conducted on every partner. Analysis female and male biomarkers, including ART reproductive outcomes was conducted considering age as main cofounder factor. Participants/materials, setting, methods Participants were couples presenting with either primary or secondary infertility at City Fertility, Notting Hill, Melbourne. Oocytes from patients of different age were analysed using immunocytochemistry for pATM and ɣH2AX, including NAD(P)H and FAD levels measured at the GV or MI phase using a confocal microcopy (Olympus FV1200). Images were analysed using FIJI software (v2.0.0-rc-69/1.52n), using arbitrary units. Linear mixed models were employed to determine the relationship between DNA repair and metabolic biomarkers with age. Main results and the role of chance GV oocytes exhibited nuclear expression for ɣH2AX and pATM, whereas MI oocytes displayed nuclear expression for ɣH2AX, with pATM showing a cytosolic localization post-GVBD (Germinal Vesicle Breakdown). Investigation of metabolic cofactors revealed that NAD(P)H and FAD were expressed in the cytosol, with higher expression in mitochondria, particularly for FAD. Examination of the association between age and molecular markers in immature human oocytes showed no correlation between age and ɣH2AX (n = 49). However, a significant positive relationship between age and pATM was observed in MI oocytes (p = 0.010). In the context of metabolic cofactors, the optical redox ratio [ORR: NAD(P)H/ NAD(P)H + FAD] had a significant negative relationship in MI oocytes (p = 0.020), but no ","PeriodicalId":13003,"journal":{"name":"Human reproduction","volume":"23 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144513094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"P-302 Salpingoscopy combined with methylene blue dye test for evaluating tubal conditions in hydrosalpinx patients and its correlation with post-salpingoplasty pregnancy outcomes","authors":"X Yu, H Luo","doi":"10.1093/humrep/deaf097.610","DOIUrl":"https://doi.org/10.1093/humrep/deaf097.610","url":null,"abstract":"Study question Assessment of tubal conditions in hydrosalpinx patients using salpingoscopy and Methylene blue dye test: association with post-salpingoplasty pregnancy outcomes. Summary answer Salpingoscopic characteristics of hydrosalpinx patients pinpointing tubal risk factors for infertility, including methylene blue staining and the presence of foreign materials. What is known already To assess the efficacy of salpingoscopy in evaluating tubal conditions in patients diagnosed with hydrosalpinx, in conjunction with the methylene blue dye test, and to explore the correlation of these evaluations with pregnancy outcomes following salpingoplasty. The findings are intended to offer new insights into the diagnosis and management of infertility. Study design, size, duration A retrospective analysis was performed on a cohort of 146 infertile patients who underwent laparoscopy and salpingoscopy at Peking University People’s Hospital from Oct 2021 to Sep 2023.Clinical and pathological data were systematically collected, encompassing fallopian tube characteristics, patient age, obstetric history, preoperative levels of Anti-Müllerian Hormone (AMH), Follicle-Stimulating Hormone (FSH), carbohydrate antigen 125 (CA125), and pregnancy outcomes. Participants/materials, setting, methods All patients underwent salpingoscopy during laparoscopic procedures. The distal end of the hydrosalpinx was surgically opened to facilitate visualization. Observations during salpingoscopy were meticulously recorded, including the tubal mucosa, and the luminal adhesions, foreign materials, and other notable features. Following this, a 2.8% methylene blue dye was introduced, and the tube was re-evaluated after three minutes to assess dye uptake via salpingoscopy. Tubal conditions were quantified using a scoring system based on the aforementioned observations. Main results and the role of chance Among the 146 patients included, the pregnancy rate was 43.8% and the hydrosalpinx recurrence rate was 5.5%. Methylene blue staining of the fallopian tube was identified as a significant risk factor for postoperative infertility (Z = -3.208, P = 0.001). The presence of foreign material within the tube was significantly associated with preoperative infertility (Z = 0.494, P < 0.001). A positive correlation was observed between tubal mucosal scores and adhesion scores (r = 0.374, P < 0.001). Age was negatively correlated with preoperative AMH levels (r = -0.436, P < 0.001) but positively correlated with the number of prior pregnancies (r = 0.322, P < 0.001) and deliveries (r = 0.274, P = 0.001). FSH levels were positively correlated with tubal mucosal status (r = 0.300, P = 0.026), the presence of foreign material (r = 0.390, P = 0.003), and the overall tubal score (r = 0.334, P = 0.013). Conversely, AMH levels were negatively correlated with the overall tubal score (r = -0.246, P = 0.018). Limitations, reasons for caution However, further research with larger sa","PeriodicalId":13003,"journal":{"name":"Human reproduction","volume":"38 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144513111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}