Human reproduction最新文献

{"title":"One-year cumulative live birth rate associated with the number of oocytes in ovarian stimulation with follitropin delta: a pooled analysis of four randomized controlled trials.","authors":"Rita Lobo, Samuel Santos-Ribeiro, Ali Falahati, Kelle Moley, Anja Pinborg, Nick S Macklon, Ida E Jepsen","doi":"10.1093/humrep/deaf111","DOIUrl":"10.1093/humrep/deaf111","url":null,"abstract":"<p><strong>Study question: </strong>What number of oocytes retrieved is associated with the highest cumulative live birth rates (CLBRs) in the fresh and subsequent frozen cycles following ovarian stimulation with follitropin delta?</p><p><strong>Summary answer: </strong>The CLBR increased with the number of oocytes retrieved, plateauing at 21-25 oocytes.</p><p><strong>What is known already: </strong>Live birth rate (LBR) per fresh cycle is the conventionally reported outcome of IVF; however, the marked increase in cryopreserved cycles in recent years suggests that the CLBR has emerged as a more relevant outcome. In the fresh cycle, the number of oocytes retrieved is regarded as a prognostic factor for LBR, and a similar association has been shown for CLBR.</p><p><strong>Study design, size, duration: </strong>Pooled analysis including 1746 patients from four randomized controlled trials. Trials were identified from clinical trials available in the Ferring Pharmaceuticals database up to June 2023. Selected trials used follitropin delta for ovarian stimulation and collected outcome data from both fresh and frozen cycles. Follitropin delta dose-response trials, as well as trials investigating follitropin delta in repeated ovarian stimulation cycles, were excluded. Patients included in the analysis underwent ovarian stimulation with follitropin delta and had at least one oocyte retrieved. The outcome of CLBR in the fresh and subsequent frozen cycles was evaluated in relation to the number of oocytes retrieved. CLBR was calculated as the number of patients with at least one live birth divided by the number of all patients included in the analysis.</p><p><strong>Participants/materials, setting, methods: </strong>Trial participants were women, 18-42 years of age, who were undergoing their first or second IVF/ICSI cycle in a GnRH antagonist/agonist protocol. Triggering was performed with hCG or GnRH agonist, and insemination was performed by IVF or ICSI. Single or double blastocyst transfer was performed on Day 5 in the fresh cycle, and all viable surplus blastocysts were cryopreserved on Day 5 or Day 6. All pregnancies from the fresh cycle and frozen cycles initiated within 1 year after the start of stimulation were followed until birth. The association between the number of oocytes retrieved and CLBR was assessed using a logistic regression analysis with fractional polynomials to obtain predicted CLBR. Subgroup analyses were performed based on age, anti-Müllerian hormone (AMH), and number of oocytes retrieved.</p><p><strong>Main results and the role of chance: </strong>Overall, 15 trials with follitropin delta were identified in the database. Of those, 11 trials were not eligible, and the remaining 4 trials were included. In total, 1746 patients were included in the analysis. The mean age was 33.8 years (range 21-42 years), and the median AMH level was 17.0 pmol/l (range 0.3-164.2 pmol/l). The vast majority of patients (1645 patients, 94.2%) were treate","PeriodicalId":13003,"journal":{"name":"Human reproduction","volume":" ","pages":"1526-1534"},"PeriodicalIF":6.1,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12314149/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144283739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The importance of understanding the human follicle reserve.","authors":"Roseanne Rosario, Evelyn E Telfer, Raymond J Rodgers, Richard A Anderson","doi":"10.1093/humrep/deaf104","DOIUrl":"10.1093/humrep/deaf104","url":null,"abstract":"","PeriodicalId":13003,"journal":{"name":"Human reproduction","volume":" ","pages":"1587-1588"},"PeriodicalIF":6.1,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144215692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rescue in vitro maturation of germinal vesicle oocytes after ovarian stimulation: the importance of the culture media.","authors":"Nuria Soler, Danilo Cimadomo, Laura Escrich, Noelia Grau, Arancha Galán, Pilar Alamá, María José de Los Santos, Laura Rienzi, María José Escribá","doi":"10.1093/humrep/deaf099","DOIUrl":"10.1093/humrep/deaf099","url":null,"abstract":"<p><strong>Study question: </strong>What are the nuclear and initial developmental outcomes of rescue-IVM oocytes across multiple commercially available media?</p><p><strong>Summary answer: </strong>Of the 11 different media tested, Medium K provided the highest rescue rate in the shortest time and elicited the highest normal oocyte activation rate (NOAR).</p><p><strong>What is known already: </strong>Following ovarian stimulation, 10-15% of the oocytes are immature with about 10% states in prophase I, i.e. the germinal vesicle (GV) stage. A consensus is still needed whether to rescue these oocytes for clinical use or not, as reports of live births from rescued-metaphase-II (MII) oocytes continues to emerge. These oocytes may be valuable to poor prognosis patients when alternatives are not available and to oncofertility patients. Nonetheless, although rescue in vitro maturation (r-IVM) is experiencing a comeback, clear good practice recommendations regarding inherent protocols are lacking. Moreover, no commercially available culture media exist to support this potentially valuable rescue strategy.</p><p><strong>Study design, size, duration: </strong>A prospective experimental study was conducted in a 1-year period at a private IVF center and entailing two consecutive phases. In study phase I, 1570 GV oocytes retrieved after ovarian stimulation from 490 young donors (maximum 4 per donor) were randomly cultured for 24 h in 11 commercially available culture media in a time lapse incubator. The two media eliciting the highest rescue rate in the shortest time were selected for study phase II. In this phase, 105 r-MII oocytes, obtained from 190 GVs rescued in the two chosen media, underwent artificial oocyte activation (AOA) and were further cultured in a time lapse incubator for 24 additional hours in Medium J until time of pronuclear fading (tPNf).</p><p><strong>Participants/materials, setting, methods: </strong>All donors (26.1 ± 3.8 years) underwent GnRH antagonist ovarian stimulation protocols with agonist trigger. The oocyte in vivo maturation rate was 80%. In the case of immature oocytes, two to four GV per woman were donated for research and cultured in an time lapse incubator. Time of GV breakdown (tGVBD) and time of the first polar body extrusion (t1PB) were annotated. AOA was conducted according to a previously published protocol. The primary outcome of study phase I was the rescue rate per cultured GV and the nuclear maturation dynamics in each medium studied. The primary outcome of study phase II was the NOAR of r-IVM oocytes in each selected medium. In this phase, time of pronuclear appearance and fading (tPNa and tPNf) and S-phase duration were also annotated.</p><p><strong>Main results and the role of chance: </strong>The GVs cultured in 3 of the 11 tested media showed rescue rates ≥55%. However, the time to reach the r-MII in two of them, namely Medium G and Medium K, was significantly shorter (19.4 ± 0.2 h, 95%CI: 19.0-19.8 h). These ","PeriodicalId":13003,"journal":{"name":"Human reproduction","volume":" ","pages":"1504-1515"},"PeriodicalIF":6.1,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12314148/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Are open-identity donors prepared for release of their identity? Long-term follow-up of a national sample of oocyte and sperm donors","authors":"Claudia Lampic, Emilia Thorup, Marie Bladh, Elizabeth Nedstrand, Xana Brinck, Agneta Skoog Svanberg, Gunilla Sydsjö","doi":"10.1093/humrep/deaf134","DOIUrl":"https://doi.org/10.1093/humrep/deaf134","url":null,"abstract":"STUDY QUESTION What are the perspectives of oocyte and sperm donors 14–17 years post-donation on the release of their identity and potential contact with donor-conceived offspring (DCO)? SUMMARY ANSWER Most oocyte and sperm donors wanted to be notified about future releases of their identity and were positive towards contact with DCO, but more than half expressed a need for support in relation to potential contact. WHAT IS KNOWN ALREADY Worldwide, an increasing number of individuals conceived by open-identity donation are reaching an age where they may request donor identity. Little is known regarding donors’ preparedness for identity-release and potential contact with DCO. STUDY DESIGN, SIZE, DURATION This study is part of the ‘Swedish Study on Gamete Donation’ (SSGD), a longitudinal multicentre study including oocyte and sperm donors at all clinics performing gamete donation in Sweden. Consecutive recruitment during a 3-year period (2005–2008) resulted in an initial sample of 299 donors (80% response rate). The present study concerns the fifth wave of data collection of the SSGD conducted 14–17 years post-donation, with very high response rates (oocyte donor 83%, sperm donor 92%). Following exclusion of donors who knew the recipients and/or knew that the donation had not resulted in a living child, the final sample comprised 100 oocyte donor and 91 sperm donor. PARTICIPANTS/MATERIALS, SETTING, METHODS Fourteen to seventeen years after having participated in open-identity donation of their gametes, participants completed a postal survey with study-specific questions. Questions concerned preferences related to the release of their identity to DCO, attitudes towards future contact with people conceived from their donations, need for support regarding potential contact, attitudes towards the perceived importance of the genetic link between parent and child, and openness about having donated oocytes or sperm. Chi-square tests, independent t-tests, and Mann–Whitney U-tests were used to compare responses between oocyte and sperm donors. Multinomial logistic regression was used to identify factors associated with donors’ attitudes towards future contact with DCO and need for support. Content analysis was used to analyse free-text responses. MAIN RESULTS AND THE ROLE OF CHANCE Almost all oocyte and sperm donors wanted to be notified about requests for their identity (93%). A majority had positive (71%) or neutral (19%) attitudes towards contact with DCO, but a small group was negative (10%), and more than half wanted support related to potential contact (59%). Free-text responses indicated that donors took the interests of both the DCO and their own family members into account when considering future contact. Donors’ attitudes towards contact with people conceived from their donations and donors’ need for support were not predicted by socio-demographic factors such as donors’ gender and legal children, nor by the perceived importance of the genetic pare","PeriodicalId":13003,"journal":{"name":"Human reproduction","volume":"9 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144755868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harmine inhibits oxidative phosphorylation, thus regulating the polarization of macrophages mediated by extracellular adenosine in endometriosis.","authors":"Anwei Lu,Shuying Lin,Jiajing Li,Haijuan Luo,Chengyu Lu,Yuzhen Wang,Yuye Cao,Xiaojuan Cao,Jiaxin Peng,Jiaming Zhang,Li Zhou,Minuo Yin","doi":"10.1093/humrep/deaf130","DOIUrl":"https://doi.org/10.1093/humrep/deaf130","url":null,"abstract":"STUDY QUESTIONCan harmine mitigate endometriosis by modulating oxidative phosphorylation (OXPHOS) suppression and macrophage polarization?SUMMARY ANSWERHarmine induces apoptosis in endometriotic lesions by inhibiting OXPHOS while also modulating macrophage polarization through the reduction of extracellular adenosine (eADO), a metabolic byproduct of OXPHOS.WHAT IS KNOWN ALREADYEndometriosis is associated with mitochondrial dysfunction while ectopic endometrial stromal cells (EESCs) exhibit heightened susceptibility to oxidative stress. Macrophages accumulate in the peritoneal cavity and peritoneal fluid, constituting a dominant immune cell population within endometriotic lesions.STUDY DESIGN, SIZE, DURATIONA total of 25 patients diagnosed with ovarian endometriosis and 25 healthy donors undergoing surgical treatment at a university-affiliated hospital within 6 months were included. Primary normal endometrial stromal cells (NESCs) and EESCs were isolated, cultured, and subjected to pharmacological drug intervention, small interfering RNA (siRNA) transfection, OXPHOS analysis, and transcriptomic profiling. Macrophage cell line RAW264.7 was used to study macrophage polarization. The in vivo study involved 24 C57BL/6 female mice to establish an endometriosis model.PARTICIPANTS/MATERIALS, SETTING, METHODSThe impact of harmine on cellular viability in vitro was assessed using Cell Counting Kit-8 (CCK-8) clonogenic assays, 5-ethynyl-2'-deoxyuridine (EdU) incorporation, and flow cytometry. OXPHOS inhibition was evaluated through mitochondrial ultrastructural changes observed via transmission electron microscopy and fluorescence staining. Oxidative stress was quantified using JC-1 mitochondrial membrane potential assays and reactive oxygen species (ROS) detection kits. Seahorse oxygen consumption rate assay was conducted to measure OXPHOS activity. Immunohistochemistry was employed to examine key protein expression in endometrial tissues. ATP and eADO levels were quantified using an ATP Assay Kit and Adenosine Assay Kit, respectively. We used RNA sequencing (RNA-seq) to identify enriched biological pathways. Flow cytometry was used to characterize macrophage populations in peritoneal fluid, and CIBERSORTx software was applied to generate immune cell expression profiles. Samples with P-values <0.05 were selected for analysis, with macrophage subtype (Mφ2/Mφ1) alterations statistically evaluated using the Wilcoxon test.MAIN RESULTS AND THE ROLE OF CHANCEEESCs exhibited greater susceptibility to harmine treatment compared to NESCs (P < 0.05). RNA-seq analysis revealed that harmine primarily influences pathways related to cell proliferation, oxidative stress, and HIF-1 signaling. Differentially expressed genes were enriched in complexes regulating mitochondrial OXPHOS. Harmine intervention significantly increased mitochondrial fission in EESCs compared to NESCs and markedly suppressed OXPHOS activity in EESCs. Treatment with harmine decreased total ROS levels","PeriodicalId":13003,"journal":{"name":"Human reproduction","volume":"13 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Double trouble: the impact of repeated biopsy and vitrification on euploid blastocyst transfer outcomes.","authors":"George Liperis,Kashish Sharma,Juan J Fraire-Zamora,Munevver Serdargullari,Alison Campbell,Andrew Thomson,Alessandra Alteri,Debbie Montjean,Omar F Ammar","doi":"10.1093/humrep/deaf146","DOIUrl":"https://doi.org/10.1093/humrep/deaf146","url":null,"abstract":"","PeriodicalId":13003,"journal":{"name":"Human reproduction","volume":"47 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144693313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial intelligence-simplified information to advance reproductive genetic literacy and health equity.","authors":"Marjan Naghdi,Ping Cao,Rick Essers,Malou Heijligers,Aimee D C Paulussen,Arie van der Lugt,Robert A C Ruiter,Wendy A G van Zelst-Stams,Andres Salumets,Masoud Zamani Esteki","doi":"10.1093/humrep/deaf135","DOIUrl":"https://doi.org/10.1093/humrep/deaf135","url":null,"abstract":"STUDY QUESTIONCan artificial intelligence (AI) and large language models (LLMs) effectively simplify patient education materials (PEMs) to advance reproductive genetic literacy and health equity?SUMMARY ANSWERLLMs offer a promising approach to support healthcare professionals in generating effective, and simplified PEMs.WHAT IS KNOWN ALREADYReproductive genetic testing and counseling holds the potential to support a personalized approach to reduce the burden of genetic disorders. However, its uptake remains limited due to the complexity of the tests and the way that PEMs have been designed. This is more prominent in reproductive genetic testing, as vulnerability of patients may lead to over- or under-use of genetic testing technologies.STUDY DESIGN, SIZE, DURATIONWe carried out a comparative observational study to evaluate the capacity of four AI/LLMs to simplify PEMs (n = 30) in reproductive genetics and assessing the clinical accuracy of simplified versions (n = 120) by experts (n = 30). Additionally, we devised a graphical user interface (GUI) to support real-time text simplification and readability analysis.PARTICIPANTS/MATERIALS, SETTING, METHODSWe collected 30 PEMs covering six topics in reproductive genetics from well-recognized platforms, such as WHO, MedlinePlus, and Johns Hopkins. Each PEM was processed by four AI/LLMs (GPT-3.5, GPT-4, Copilot, Gemini) using a fixed prompt, resulting in 120 simplified outputs. We measured readability improvements using five validated metrics, such as simple measure of gobbledygook, each capturing distinct textual characteristics such as sentence length and word complexity. To evaluate clinical reliability of the simplified outputs, a panel of experts (n = 30) in reproductive genetics independently scored each text (3 per text).MAIN RESULTS AND THE ROLE OF CHANCEAll four LLMs significantly improved the readability of the PEMs (P-values <0.001), reducing text complexity to an average 6th-7th grade reading level. While Gemini and Copilot achieved the highest improvement in readability scores, GPT-4 received the highest expert rating across all criteria-accuracy (4.1 ± 0.9), completeness (4.2 ± 0.8), and relevance of omissions (4.0 ± 0.9; P < 10-8). These findings highlight the importance of balancing readability with content integrity to support informed decision-making, as excessive simplification may compromise essential medical information. We devised an open-access GUI that provides real-time PEM simplification and readability analysis to support the integration of AI-assisted approaches in clinical practice (https://huggingface.co/spaces/CellularGenomicMedicine/HealthLiteracyEvaluator).LIMITATIONS, REASONS FOR CAUTIONCareful evaluation of LLM-simplified PEMs is required to ensure that simplification does not lead to omission of critical information. In addition, in this study, we report only the readability improvements of AI-generated texts and expert evaluations. To truly assess the potential of the","PeriodicalId":13003,"journal":{"name":"Human reproduction","volume":"14 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144677976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing fertility education to address changing societal and demographic trends.","authors":"Münevver Serdarogullari,Georgios Liperis,Sofia Makieva,Claudia Massarotti,Juan J Fraire-Zamora,Kashish Sharma,Bola Grace,Anita Fincham,Juliana Pedro,Zoya Ali","doi":"10.1093/humrep/deaf125","DOIUrl":"https://doi.org/10.1093/humrep/deaf125","url":null,"abstract":"","PeriodicalId":13003,"journal":{"name":"Human reproduction","volume":"30 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144677972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-inferior or not inferior, it is more than semantics.","authors":"Baris Ata,Erkan Kalafat,Barbara Lawrenz,Human Fatemi","doi":"10.1093/humrep/deaf140","DOIUrl":"https://doi.org/10.1093/humrep/deaf140","url":null,"abstract":"","PeriodicalId":13003,"journal":{"name":"Human reproduction","volume":"17 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144677973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reply: Non-inferior or not inferior, it is more than semantics.","authors":"He Cai,Danmeng Liu,Zan Shi,Juanzi Shi,Li Tian","doi":"10.1093/humrep/deaf141","DOIUrl":"https://doi.org/10.1093/humrep/deaf141","url":null,"abstract":"","PeriodicalId":13003,"journal":{"name":"Human reproduction","volume":"45 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144678106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}