Gut Microbes最新文献_第5页

Enterotoxigenic Escherichia coli heat labile enterotoxin affects neutrophil effector functions via cAMP/PKA/ERK signaling. 肠毒性大肠埃希氏菌热溶性肠毒素通过 cAMP/PKA/ERK 信号转导影响中性粒细胞效应功能

IF 12.2 1区医学

Gut Microbes Pub Date : 2024-09-16 DOI: 10.1080/19490976.2024.2399215

Jinglin Ma,Leen Hermans,Matthias Dierick,Hans Van der Weken,Eric Cox,Bert Devriendt

{"title":"Enterotoxigenic Escherichia coli heat labile enterotoxin affects neutrophil effector functions via cAMP/PKA/ERK signaling.","authors":"Jinglin Ma,Leen Hermans,Matthias Dierick,Hans Van der Weken,Eric Cox,Bert Devriendt","doi":"10.1080/19490976.2024.2399215","DOIUrl":"https://doi.org/10.1080/19490976.2024.2399215","url":null,"abstract":"Enterotoxigenic Escherichia coli (ETEC) are a major cause of diarrheal illness in humans and animals, induced by enterotoxins produced by these pathogens. Despite the crucial role of neutrophils in combatting bacterial infections, our understanding of how enterotoxins impact neutrophil function is limited. To address this knowledge gap, we used heat-labile enterotoxin (LT) and heat-stable enterotoxin a (STa) to investigate their impact on the effector functions of neutrophils. Our study reveals that pSTa does not exert any discernible effect on the function of neutrophils. In contrast, LT altered the migration and phagocytosis of neutrophils and induced the production of inflammatory factors via activation of cAMP/PKA and ERK1/2 signaling. LT also attenuated the release of neutrophil extracellular traps by neutrophils via the PKA signaling pathway. Our findings provide novel insights into the impact of LT on neutrophil function, shedding light on the underlying mechanisms that govern its immunoregulatory effects. This might help ETEC in subverting the immune system and establishing infection.","PeriodicalId":12909,"journal":{"name":"Gut Microbes","volume":"23 1","pages":"2399215"},"PeriodicalIF":12.2,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142245230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gut microbiota-mediated C-sulfonate metabolism impairs the bioavailability and anti-cholestatic efficacy of andrographolide. 肠道微生物群介导的C-磺酸盐代谢会损害穿心莲内酯的生物利用度和抗胆汁淤积功效。

IF 12.2 1区医学

Gut Microbes Pub Date : 2024-09-12 DOI: 10.1080/19490976.2024.2387402

Dafu Tang,Wanyu Hu,Bingxuan Fu,Xiaojie Zhao,Guoquan You,Cong Xie,Hong Yu Wang,Xueni Guo,Qianbing Zhang,Zhongqiu Liu,Ling Ye

{"title":"Gut microbiota-mediated C-sulfonate metabolism impairs the bioavailability and anti-cholestatic efficacy of andrographolide.","authors":"Dafu Tang,Wanyu Hu,Bingxuan Fu,Xiaojie Zhao,Guoquan You,Cong Xie,Hong Yu Wang,Xueni Guo,Qianbing Zhang,Zhongqiu Liu,Ling Ye","doi":"10.1080/19490976.2024.2387402","DOIUrl":"https://doi.org/10.1080/19490976.2024.2387402","url":null,"abstract":"Cholestatic liver injury results from the accumulation of toxic bile acids in the liver, presenting a therapeutic challenge with no effective treatment available to date. Andrographolide (AP) has exhibited potential as a treatment for cholestatic liver disease. However, its limited oral bioavailability poses a significant obstacle to harnessing its potent therapeutic properties and restricts its clinical utility. This limitation is potentially attributed to the involvement of gut microbiota in AP metabolism. In our study, employing pseudo-germ-free, germ-free and strain colonization animal models, along with 16S rRNA and shotgun metagenomic sequencing analysis, we elucidate the pivotal role played by gut microbiota in the C-sulfonate metabolism of AP, a process profoundly affecting its bioavailability and anti-cholestatic efficacy. Subsequent investigations pinpoint a specific enzyme, adenosine-5'-phosphosulfate (APS) reductase, predominantly produced by Desulfovibrio piger, which catalyzes the reduction of SO42- to HSO3-. HSO3- subsequently interacts with AP, targeting its C=C unsaturated double bond, resulting in the formation of the C-sulfonate metabolite, 14-deoxy-12(R)-sulfo andrographolide (APM). Inhibition of APS reductase leads to a notable enhancement in AP bioavailability and anti-cholestatic efficacy. Furthermore, employing RNA sequencing analysis and farnesoid X receptor (FXR) knockout mice, our findings suggest that AP may exert its anti-cholestatic effects by activating the FXR pathway to promote bile acid efflux. In summary, our study unveils the significant involvement of gut microbiota in the C-sulfonate metabolism of AP and highlights the potential benefits of inhibiting APS reductase to enhance its therapeutic effects. These discoveries provide valuable insights into enhancing the clinical applicability of AP as a promising treatment for cholestatic liver injury.","PeriodicalId":12909,"journal":{"name":"Gut Microbes","volume":"15 1","pages":"2387402"},"PeriodicalIF":12.2,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142231280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fecal microbiota transplantation derived from mild cognitive impairment individuals impairs cerebral glucose uptake and cognitive function in wild-type mice: Bacteroidetes and TXNIP-GLUT signaling pathway. 来自轻度认知障碍患者的粪便微生物群移植会损害野生型小鼠的脑葡萄糖摄取和认知功能：类杆菌和 TXNIP-GLUT 信号通路。

IF 12.2 1区医学

Gut Microbes Pub Date : 2024-09-12 DOI: 10.1080/19490976.2024.2395907

Tao Wang,Ling Hao,Kexin Yang,Wenjing Feng,Zhiting Guo,Miao Liu,Rong Xiao

{"title":"Fecal microbiota transplantation derived from mild cognitive impairment individuals impairs cerebral glucose uptake and cognitive function in wild-type mice: Bacteroidetes and TXNIP-GLUT signaling pathway.","authors":"Tao Wang,Ling Hao,Kexin Yang,Wenjing Feng,Zhiting Guo,Miao Liu,Rong Xiao","doi":"10.1080/19490976.2024.2395907","DOIUrl":"https://doi.org/10.1080/19490976.2024.2395907","url":null,"abstract":"Gut microbiome dysbiosis has been widely implicated in cognitive impairment, but the identity of the specific bacterial taxa and mechanisms are not fully elucidated. Brain glucose hypometabolism coincides with the cognitive decline. This study explored the link among cognition, gut microbiota and glucose uptake based on the fecal microbiota transplantation from mild cognitive impairment individuals (MCI-FMT) and investigated whether similar mechanisms were involved in 27-hydroxycholesterol (27-OHC)-induced cognitive decline. Our results showed that the MCI-FMT mice exhibited learning and memory decline and morphological lesions in the brain and colon tissues. There were reduced 18F-fluorodeoxyglucose uptake, downregulated expression of glucose transporters (GLUT1,3,4) and upregulated negative regulator of glucose uptake (TXNIP) in the brain. MCI-FMT altered the bacterial composition and diversity of the recipient mice, and the microbial signatures highlighted by the increased abundance of Bacteroides recapitulated the negative effects of MCI bacterial colonization. However, inhibiting Bacteroidetes or TXNIP increased the expression of GLUT1 and GLUT4, significantly improving brain glucose uptake and cognitive performance in 27-OHC-treated mice. Our study verified that cognitive decline and abnormal cerebral glucose uptake were associated with gut microbiota dysbiosis; we also revealed the involvement of Bacteroidetes and molecular mechanisms of TXNIP-related glucose uptake in cognitive deficits caused by 27-OHC.","PeriodicalId":12909,"journal":{"name":"Gut Microbes","volume":"14 1","pages":"2395907"},"PeriodicalIF":12.2,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142174727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gut microbiota mediate early life stress-induced social dysfunction and anxiety-like behaviors by impairing amino acid transport at the gut 肠道微生物群通过损害肠道氨基酸转运介导早期生活压力诱发的社交功能障碍和焦虑样行为

IF 12.2 1区医学

Gut Microbes Pub Date : 2024-09-11 DOI: 10.1080/19490976.2024.2401939

Jiushuang Zhu, Zhuoting Zhong, Lijie Shi, Ling Huang, Chunqiao Lin, Yan He, Xiuwen Xia, Tiane Zhang, Weijun Ding, Youjun Yang

引用次数: 0

Segmental patterning of microbiota and immune cells in the murine intestinal tract. 小鼠肠道微生物群和免疫细胞的节段模式。

IF 12.2 1区医学

Gut Microbes Pub Date : 2024-09-10 DOI: 10.1080/19490976.2024.2398126

Harithaa Anandakumar,Ariana Rauch,Moritz I Wimmer,Alex Yarritu,Gudrun Koch,Victoria McParland,Hendrik Bartolomaeus,Nicola Wilck

{"title":"Segmental patterning of microbiota and immune cells in the murine intestinal tract.","authors":"Harithaa Anandakumar,Ariana Rauch,Moritz I Wimmer,Alex Yarritu,Gudrun Koch,Victoria McParland,Hendrik Bartolomaeus,Nicola Wilck","doi":"10.1080/19490976.2024.2398126","DOIUrl":"https://doi.org/10.1080/19490976.2024.2398126","url":null,"abstract":"The intestine exhibits distinct characteristics along its length, with a substantial immune cell reservoir and diverse microbiota crucial for maintaining health. This study investigates how anatomical location and regional microbiota influence intestinal immune cell abundance. Using conventionally colonized and germ-free mice, segment-specific immune cell composition and microbial communities were assessed. Metagenomic sequencing analyzed microbiome variations, while flow cytometry and immunofluorescence examined immune cell composition. Microbiome composition varied significantly along the intestine, with diversity and abundance increasing from upper to lower segments. Immune cells showed distinct segment-specific patterning influenced by microbial colonization and localization. T cell subsets displayed varied dependence on microbiome presence and anatomical location. This study highlights locoregional differences in intestinal immune cell and microbiome composition, identifying immune subsets susceptible to microbiota presence. The findings provide context for understanding immune cell alterations in disease models.","PeriodicalId":12909,"journal":{"name":"Gut Microbes","volume":"24 1","pages":"2398126"},"PeriodicalIF":12.2,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142165920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbiome 2.0: lessons from the 2024 Gut Microbiota for Health World Summit. 微生物组 2.0：2024 年 "肠道微生物群促进健康 "世界峰会的经验教训。

IF 12.2 1区医学

Gut Microbes Pub Date : 2024-09-10 DOI: 10.1080/19490976.2024.2400579

Sushrut Jangi,Gail Hecht

{"title":"Microbiome 2.0: lessons from the 2024 Gut Microbiota for Health World Summit.","authors":"Sushrut Jangi,Gail Hecht","doi":"10.1080/19490976.2024.2400579","DOIUrl":"https://doi.org/10.1080/19490976.2024.2400579","url":null,"abstract":"This Meeting Summary highlights the key insights from the 12th meeting of the Gut Microbiota for Health World Summit, held in Washington, DC, organized by the American Gastroenterological Association (AGA) and the European Society of Neurogastroenterology and Motility (ESNM). Through a 2-day series of plenary sessions, workshops, a poster session, and live discussions involving thought leaders, physicians, researchers, and representatives from the Food and Drug Administration and the pharmaceutical industry, the conference attendees focused on the strategies and challenges in developing microbiome-based therapies to prevent and treat human disease. The conference highlighted progress in the field, including the recently successful introduction of 2 new fecal microbial transplantation-based products into the clinical setting, and the continuing development of next-generation probiotics. However, to continue to advance microbiome-directed treatments, three key themes emerged during the meeting, including (1) better methods to identify actionable targets in the microbiome (2) developing effective strategies to manipulate the microbiome (3) aligning microbiome-based therapies with existing treatment paradigms in the real world.","PeriodicalId":12909,"journal":{"name":"Gut Microbes","volume":"31 1","pages":"2400579"},"PeriodicalIF":12.2,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142165921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactobacillus acidophilus ameliorates cholestatic liver injury through inhibiting bile acid synthesis and promoting bile acid excretion 嗜酸乳杆菌通过抑制胆汁酸合成和促进胆汁酸排泄改善胆汁淤积性肝损伤

IF 12.2 1区医学

Gut Microbes Pub Date : 2024-08-29 DOI: 10.1080/19490976.2024.2390176

Lingyi Wu, Jianchun Zhou, An Zhou, Yuanyuan Lei, Li Tang, Shiping Hu, Sumin Wang, Xu Xiao, Qiao Chen, Dianji Tu, Cheng Lu, Yi Lai, Yiding Li, Xiao Zhang, Bo Tang, Shiming Yang

引用次数: 0

Supplier-origin gut microbiomes affect host body weight and select autism-related behaviors 供应商来源的肠道微生物群影响宿主体重和自闭症相关行为的选择

IF 12.2 1区医学

Gut Microbes Pub Date : 2024-08-06 DOI: 10.1080/19490976.2024.2385524

Zachary L McAdams, Kevin L Gustafson, Amber L Russell, Rachel Self, Amy L Petry, Teresa E Lever, Aaron C Ericsson

引用次数: 0

AUF1-mediated inhibition of autophagic lysosomal degradation contributes to CagA stability and Helicobacter pylori-induced inflammation AUF1 介导的自噬溶酶体降解抑制有助于 CagA 的稳定性和幽门螺旋杆菌诱发的炎症

IF 12.2 1区医学

Gut Microbes Pub Date : 2024-07-28 DOI: 10.1080/19490976.2024.2382766

Huiling Zheng, Ting Zhang, Jing Zhang, Jing Ning, Weiwei Fu, Ye Wang, Yanyan Shi, Guochao Wei, Jing Zhang, Xiangmei Chen, Shigang Ding

引用次数: 0

Gut microbial features and dietary fiber intake predict gut microbiota response to resistant starch supplementation 肠道微生物特征和膳食纤维摄入量可预测肠道微生物群对抗性淀粉补充剂的反应