HereditasPub Date : 2025-06-07DOI: 10.1186/s41065-025-00464-x
Li Yan, Jiang-Han Li, Ai-Li Zhang, He Li, Bo Pang, De-Yang Meng, Qian Fu, Li-Juan Du, Yan Su
{"title":"The potential role of biomarkers CD28 and PF4 in the pathogenesis of idiopathic pulmonary fibrosis and their impact on the prognosis: an immune microenvironment analysis.","authors":"Li Yan, Jiang-Han Li, Ai-Li Zhang, He Li, Bo Pang, De-Yang Meng, Qian Fu, Li-Juan Du, Yan Su","doi":"10.1186/s41065-025-00464-x","DOIUrl":"10.1186/s41065-025-00464-x","url":null,"abstract":"<p><strong>Background: </strong>This study aims to identify and investigate biomarkers associated with mitochondrial-related genes (MRGs) and programmed cell death-related genes (PCDRGs) that concurrently influence the progression of idiopathic pulmonary fibrosis (IPF) and to explore the underlying biological mechanisms involved.</p><p><strong>Methods: </strong>The GSE28042 and GSE27957 datasets, comprising 1,136 MRGs and 1,548 PCDRGs, were utilized in this study. Differentially expressed genes (DEGs) between the IPF and control groups were initially identified through differential expression analysis. Subsequently, key module genes closely associated with IPF samples were selected using Weighted Gene Co-expression Network Analysis (WGCNA). Intersection genes 1 and 2 were then identified by overlapping DEGs with key module genes, MRGs, and PCDRGs. Candidate genes were further selected through Spearman correlation analysis involving intersection genes 1 and 2. Additionally, biomarkers were identified, and a risk model was developed using Cox regression analysis, proportional hazards (PH) assumption testing, and machine learning methods. Patients with IPF were stratified into high- and low-risk cohorts. Finally, functional enrichment analysis, immune infiltration analysis, regulatory network construction, and reverse transcription quantitative PCR (RT-qPCR) were conducted separately to validate the findings.</p><p><strong>Results: </strong>CD28 and PF4 were identified as biomarkers, and a risk model was established. The distinct risk cohorts exhibited differences in pathways related to hemostasis, prion diseases, and other biological processes. A significant positive correlation with was observed between CD28 and native CD4 T cells, while PF4 showed a negative correlation with activated NK cells. Based on these two biomarkers, 30 miRNAs and 532 lncRNAs were predicted, resulting in the construction of a lncRNA-miRNA-biomarker network. Additionally, 11 chemicals associated with these biomarkers were identified. RT-qPCR analysis further confirmed that expression levels of CD28 and PF4 were significantly reduced in IPF samples (P < 0.05).</p><p><strong>Conclusion: </strong>The results of this study suggested that the biomarkers CD28 and PF4 might play a potential role in the pathogenesis of IPF and might have an impact on the prognosis of the disease. These findings might offer valuable insights for future treatment strategies and prognostic evaluation for patients with IPF.</p>","PeriodicalId":12862,"journal":{"name":"Hereditas","volume":"162 1","pages":"98"},"PeriodicalIF":2.7,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12144798/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144247491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The bibliometric analysis of research on traditional Chinese medicine regulating gut microbiota for cancer treatment from 2014 to 2024.","authors":"Youfeng Lei, Yueqin Shan, Danfeng Zhou, Chunyan Chen","doi":"10.1186/s41065-025-00456-x","DOIUrl":"10.1186/s41065-025-00456-x","url":null,"abstract":"<p><strong>Objective: </strong>The modulation of gut microbiota by Traditional Chinese Medicine (TCM) offers a promising approach to cancer treatment. However, a comprehensive bibliometric evaluation of this emerging field is lacking.</p><p><strong>Objective: </strong>This study aimed to systematically analyze global research trends, hotspots, and future directions related to TCM regulation of gut microbiota in cancer therapy from 2014 to 2024.</p><p><strong>Methods: </strong>Publications were retrieved from the Web of Science Core Collection. Bibliometric and visual analyses were conducted using VOSviewer and CiteSpace to examine publication trends, country and institutional collaborations, core authors and journals, keyword co-occurrence, and research frontiers.</p><p><strong>Results: </strong>A total of 340 relevant articles were identified. The number of publications increased significantly after 2018, indicating growing interest in this field. China dominated the research landscape, both in productivity and institutional collaboration. Core research hotspots included \"short-chain fatty acids,\" \"tumor microenvironment,\" \"apoptosis,\" and \"immune response.\" Thematic evolution analysis highlighted a shift from general gut microbiota research to precise molecular mechanisms and targeted regulation. Emerging topics such as \"metabolomics\" and \"immune checkpoint blockade\" suggest future directions.</p><p><strong>Conclusion: </strong>This study provides a comprehensive overview of the current research landscape on TCM-modulated gut microbiota in cancer treatment. By identifying core contributors, research hotspots, and frontiers, it offers valuable guidance for future investigations and interdisciplinary collaborations in this promising field.</p>","PeriodicalId":12862,"journal":{"name":"Hereditas","volume":"162 1","pages":"94"},"PeriodicalIF":2.7,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12131599/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144215662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HereditasPub Date : 2025-06-03DOI: 10.1186/s41065-025-00461-0
Zhigao Zhang, Fucheng Zhang, Chuan Xue, Xiaoling Song, Yaojun Wang
{"title":"LncRNA FLG-AS1 inhibits esophageal squamous cell carcinoma by regulating the miR-23a-3p/HOXD10 axis.","authors":"Zhigao Zhang, Fucheng Zhang, Chuan Xue, Xiaoling Song, Yaojun Wang","doi":"10.1186/s41065-025-00461-0","DOIUrl":"10.1186/s41065-025-00461-0","url":null,"abstract":"<p><strong>Background: </strong>Esophageal cancer (EC) is the ninth most common cancer worldwide that kills about 300,000 people each year. Esophageal squamous cell carcinoma (ESCC) is the main type of EC. Long non-coding RNAs (lncRNAs) have been proven to be severely dysregulated in EC, but the functions of more lncRNAs still need to be explored.</p><p><strong>Methods: </strong>To explore the new molecular mechanism of ESCC development, the online biology databases (GEO, lncRNASNP2, Starbase, TargetScan) were employed to investigate the novel pathways implicated. To assess the expression levels of FLG-AS1, miR-23a-3p, and associated genes, we utilized RT-qPCR. The expression of HOXD10 was evaluated through western blotting analysis. To elucidate the regulatory interactions among FLG-AS1, miR-23a-3p, and HOXD10, a combination of dual luciferase assays, silencing techniques, and overexpression studies were conducted. The migratory and invasive capabilities of the cells were examined using a transwell apparatus. Cell viability was measured employing the CCK-8 assay, while apoptosis was detected through Annexin V/PI double staining methodology. Concentrations of glucose and lactic acid were determined utilizing appropriate biochemical kits.</p><p><strong>Results: </strong>FLG-AS1 and HOXD10 exhibited low expression levels in ESCC cells, whereas miR-23a-3p was found to be highly expressed. FLG-AS1 was observed to reduce the free level of miR-23a-3p by directly binding to it, and in turn, miR-23a-3p inhibited the expression of HOXD10 by targeting its mRNA. The overexpression of FLG-AS1 and HOXD10 resulted in the attenuation of anaerobic glycolysis, as well as a decrease in the migratory and invasive capabilities of ESCC cells, effectively reversing their resistance to cisplatin. Conversely, the upregulation of miR-23a-3p yielded opposing effects. Furthermore, ESCC patients exhibiting elevated levels of FLG-AS1 and HOXD10, alongside reduced expression of miR-23a-3p, demonstrated a significantly higher 5-year survival rate post-surgery.</p><p><strong>Conclusion: </strong>FLG-AS1 effectively inhibits the progression of ESCC and counters cisplatin resistance through the modulation of the miR-23a-3p/HOXD10 axis. This is a new mechanism affecting ESCC and will provide new ideas for the targeted therapy of ESCC.</p>","PeriodicalId":12862,"journal":{"name":"Hereditas","volume":"162 1","pages":"96"},"PeriodicalIF":2.7,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12131396/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144215685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HereditasPub Date : 2025-06-03DOI: 10.1186/s41065-025-00450-3
Dan Xuan, Xiaowan Wang, Dandan Feng, Li Wang, Yonghui Xia
{"title":"Identification of CD36 as a contributor in inflammatory response of rheumatoid arthritis and screening of feasible bioactive drugs targeting it.","authors":"Dan Xuan, Xiaowan Wang, Dandan Feng, Li Wang, Yonghui Xia","doi":"10.1186/s41065-025-00450-3","DOIUrl":"10.1186/s41065-025-00450-3","url":null,"abstract":"<p><strong>Background: </strong>Rheumatoid arthritis (RA) is a chronic inflammatory disease. This study aims to identify candidate therapeutic targets and promising drugs for RA.</p><p><strong>Methods: </strong>RA-related microarray datasets (GSE77298 and GSE206848) and inflammatory genes (IRGs) were downloaded from Gene Expression Omnibus database and GeneCards database, respectively. After removing batch effects, differentially expressed genes (DEGs) were screened using filtering criteria of P < 0.05 and |log2(fold change)|> 1. Differentially expressed IRGs (DE-IRGs) were then obtained. Key gene modules were identified by weighted gene co-expression network analysis (WGCNA), and the hub genes were then identified from the results of protein-protein interaction (PPI) network analysis, WGCNA and DE-IRGs, and validated by a external dataset GSE93272. Receiver operating characteristic (ROC) curve was used to evaluate the diagnostic effect of the predicted hub genes. In addition, drug prediction was performed through virtual screening. mRNA and protein expression of cluster of differentiation 36 (CD36) were detected by RT-qPCR and Western blot. After RA fibroblast-like synovial cells (RA-FLS) were treated with piceatannol and epicatechin, cell proliferation was detected by CCK-8 assay, and flow cytometry was used to detect cell cycle and apoptosis, and the secretion of inflammatory cytokines was detected by enzyme-linked immunosorbent assay.</p><p><strong>Results: </strong>Three hub genes were finally identified, including CD36, perilipin 1 and lipoprotein lipase. CD36 was further identified as a candidate biomarker and therapeutic target for RA, which had relatively good diagnostic efficacy for RA. Compared with fibroblast-like synovial cells (FLS), mRNA and protein expression levels of CD36 in RA-FLS were significantly up-regulated (P < 0.05). Piceatannol and epicatechin had good binding affinity with CD36 (docking score < -5 kcal/mol), and piceatannol treatment or epicatechin treatment inhibited the proliferation and inflammation of RA-FLS and induced cell cycle arrest and apoptosis (P < 0.05).</p><p><strong>Conclusion: </strong>CD36 is a potential biomarker and therapeutic target associated with synovial inflammation of RA, and piceatannol and epicatechin are potential natural drugs for RA treatment. Overall, these findings provide new insights into the clinical diagnosis and treatment of RA.</p>","PeriodicalId":12862,"journal":{"name":"Hereditas","volume":"162 1","pages":"95"},"PeriodicalIF":2.7,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12131835/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144215684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"PDE3B and HBB are key prognostic biomarkers driving cell proliferation and regulating immune microenvironment in breast cancer.","authors":"Bolong Yin, Xiangrong Luo, Xuebo Yan, Hui Shen, Jianping Jiang","doi":"10.1186/s41065-025-00470-z","DOIUrl":"10.1186/s41065-025-00470-z","url":null,"abstract":"<p><strong>Background: </strong>Breast cancer is a heterogeneous malignancy with diverse tumor subpopulations and complex tumor-immune interactions. This study explores the prognostic and functional roles of PDE3B and HBB in breast cancer, focusing on their contributions to proliferation and immune microenvironment modulation.</p><p><strong>Methods: </strong>Single-cell RNA sequencing (scRNA-seq) and TCGA data were analyzed to identify malignant subpopulations and prognostic genes. Differential gene expression, KEGG enrichment, LASSO regression, and Kaplan-Meier survival analyses were performed. Immune infiltration was assessed using EPIC deconvolution. Functional validation included qRT-PCR, IHC, Western blot, and proliferation assays in MDA-MB-231 cells.</p><p><strong>Results: </strong>Malignant cell type 3 exhibited the highest proliferative potential. PDE3B and HBB were identified as prognostic markers, strongly associated with poor survival and immune cell infiltration. Overexpression of these genes enhanced proliferation, while their knockout suppressed it.</p><p><strong>Conclusion: </strong>PDE3B and HBB drive breast cancer proliferation and immune modulation, making them promising biomarkers and therapeutic targets. Further research should assess their potential in targeted therapies.</p>","PeriodicalId":12862,"journal":{"name":"Hereditas","volume":"162 1","pages":"97"},"PeriodicalIF":2.7,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12131617/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144215661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HereditasPub Date : 2025-05-31DOI: 10.1186/s41065-025-00429-0
Mostafa A Abdel-Maksoud, Taghreed N Almana, Saeedah Almutair, Abdulaziz Alamri, Ibrahim A Saleh, Mohamed Y Zaky, Wahidah H Al-Qahtani, Yasir Hameed
{"title":"Deciphering the role of Hashimoto's Thyroiditis-related key genes in thyroid cancer via detailed in silico analysis followed by the experimental validation.","authors":"Mostafa A Abdel-Maksoud, Taghreed N Almana, Saeedah Almutair, Abdulaziz Alamri, Ibrahim A Saleh, Mohamed Y Zaky, Wahidah H Al-Qahtani, Yasir Hameed","doi":"10.1186/s41065-025-00429-0","DOIUrl":"10.1186/s41065-025-00429-0","url":null,"abstract":"<p><strong>Background: </strong>Thyroid cancer, characterized by significant genetic and epigenetic alterations, remains a critical focus of molecular oncology. This study investigates eight key genes (BRAF, EIF1 AX, FOXE1, KRAS, PDGFRA, PIK3 CA, PTEN, and TERT) that are deregulated in Hashimoto's Thyroiditis and their roles in thyroid cancer.</p><p><strong>Methods: </strong>Cell culture, nucleic acid extraction, RT-qPCR, bisulfite sequencing, and various in silico tools and databases.</p><p><strong>Results: </strong>Expression analysis using RT-qPCR revealed significant (p-value < 0.05) down-regulation of BRAF, EIF1 AX, FOXE1, KRAS, PDGFRA, PIK3 CA, PTEN, and TERT genes in thyroid cancer cell lines compared to controls, with ROC curves indicating high diagnostic accuracy (AUC 0.93-0.99). Bisulfite sequencing demonstrated increased promoter methylation across all eight genes in cancerous samples, suggesting epigenetic silencing as a regulatory mechanism. Validation through UALCAN, OncoDB, and HPA confirmed reduced gene and protein expression in additional thyroid cancer cohorts. Genetic alteration analysis via cBioPortal showed prevalent BRAF mutations, whereas other genes exhibited fewer alterations. Kaplan-Meier survival analysis linked lower expression of BRAF and PIK3 CA to poorer overall survival. Correlation studies using TISIDB and TISCH2 databases highlighted associations between gene expression and immune modulation, revealing significant correlations with immune cell infiltration and diverse immune subtypes. Moreover, miRNA-mRNA network analysis identified hsa-mir- 628 - 5p as a critical regulator targeting these genes. The impact of BRAF overexpression on SW579 cells was assessed through various functional assays. Overexpression of BRAF resulted in reduced cell proliferation, colony formation, and wound healing, which may reflect context-dependent effects. While BRAF is typically oncogenic, its overexpression may lead to cellular stress or negative feedback mechanisms that impair these processes.</p><p><strong>Conclusion: </strong>This comprehensive analysis elucidates the complex regulatory landscape of these genes in thyroid cancer, emphasizing the significant role of epigenetic modifications and providing insights into potential diagnostic and therapeutic avenues.</p>","PeriodicalId":12862,"journal":{"name":"Hereditas","volume":"162 1","pages":"91"},"PeriodicalIF":2.7,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12126899/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HereditasPub Date : 2025-05-31DOI: 10.1186/s41065-025-00457-w
Shaohua Ling, Delong Xie, Lifang Huang, Siqi Huang, Chun Tian, Liying Huang, Rong Chen, Li Qin, Xiao Qin
{"title":"Identification of EPHB4 as a potential causal gene and therapeutic target for endometriosis using Mendelian randomization.","authors":"Shaohua Ling, Delong Xie, Lifang Huang, Siqi Huang, Chun Tian, Liying Huang, Rong Chen, Li Qin, Xiao Qin","doi":"10.1186/s41065-025-00457-w","DOIUrl":"10.1186/s41065-025-00457-w","url":null,"abstract":"<p><strong>Objectives: </strong>Endometriosis is a common condition among women, characterized by chronic pain and infertility, presenting significant challenges for clinicians. This study aims to identify potential druggable targets to offer new therapeutic approaches.</p><p><strong>Method: </strong>We utilized the summary-data-based Mendelian randomization (SMR) method to investigate the causal relationships between druggable genes that encode plasma proteins and endometriosis. The data sources included the deCODE database, the UKB-PPP, and the FinnGen database. Colocalization analysis was used to identify whether candidate genes and the disease share a common causal genetic variant. Finally, we measured the protein abundance and relative mRNA expression levels of targeted druggable genes in the plasma and peripheral blood mononuclear cells (PBMCs) of endometriosis patients using ELISA and RT-qPCR.</p><p><strong>Results: </strong>By integrating the results of SMR and colocalization analyses, we found that EPHB4 is strongly associated with the risk of endometriosis, with higher levels of EPHB4 correlating with an increased risk of the condition (P<sub>FDR</sub> < 0.05, PPH4 = 0.99). RSPO3 is moderately associated, with higher levels of RSPO3 correlating with an increased risk of endometriosis (P<sub>FDR</sub> < 0.001, PPH4 = 0.78). CD109, SAA1, SAA2, FSHB, and SEZ6L2 are weakly associated with endometriosis, with higher levels of FSHB and SEZ6L2 correlating with an increased risk of endometriosis, and higher levels of CD109, SAA1, and SAA2 correlating with a decreased risk of endometriosis (P<sub>FDR</sub> < 0.05, PPH4 < 0.6). ELISA and RT-qPCR analyses showed that the EPHB4 protein abundance in plasma and mRNA expression levels in PBMCs were significantly higher in the endometriosis group compared to the control group (P-value < 0.05).</p><p><strong>Conclusions: </strong>We found that the druggable genes EPHB4, CD109, SAA1, SAA2, FSHB, and SEZ6L2 may be associated with the pathogenesis of endometriosis and are potential therapeutic targets for drug treatment. However, this preliminary study is limited by sample size and population diversity, requiring further validation to confirm the reliability of these findings.</p>","PeriodicalId":12862,"journal":{"name":"Hereditas","volume":"162 1","pages":"92"},"PeriodicalIF":2.7,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12126875/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HereditasPub Date : 2025-05-31DOI: 10.1186/s41065-025-00466-9
Ying Liao, Weiwei Zhang, Zhenfei Huang, Liu Yang, Mingjin Lu
{"title":"Diagnostic and prognostic value of miR-146b-5p in acute pancreatitis.","authors":"Ying Liao, Weiwei Zhang, Zhenfei Huang, Liu Yang, Mingjin Lu","doi":"10.1186/s41065-025-00466-9","DOIUrl":"10.1186/s41065-025-00466-9","url":null,"abstract":"<p><strong>Objective: </strong>MicroRNAs hold great potential as biomarkers for assessing the progression of acute pancreatitis (AP). This study aimed to explore the value of miR-146b-5p in the diagnosis and prognosis of AP patients.</p><p><strong>Methods: </strong>110 AP patients were included and divided into 40 severe AP (SAP) patients and 70 non-SAP patients based on disease severity. Serum miR-146b-5p levels were measured using RT-qPCR. The diagnostic value of miR-146b-5p was evaluated utilizing ROC curves. Pearson correlation coefficient was employed to analyze the correlations between APACHEII, BISAP, and MCTSI scores and miR-146b-5p levels. The AP cell model was constructed by treating AR42J cells with deoxycholic acid (DCA), the proliferative capacity of cells was measured with CCK-8, apoptosis was measured by flow cytometry, and IL-6 and IL-8 protein levels were analyzed by ELISA.</p><p><strong>Results: </strong>Serum miR-146b-5p levels were decreased in SAP and unfavorable patients. Serum miR-146b-5p was able to effectively differentiate between SAP and non-SAP patients, and also effectively differentiate between unfavorable and favorable patients. MiR-146b-5p levels were significantly negatively correlated with APACHEII score (r=-0.6676), BISAP score (r=-0.5696), and MCTSI score (r=-0.5857). Furthermore, in the AP cell model, miR-146b-5p expression was down-regulated, proliferative capacity was diminished, apoptosis was increased, and IL-6 and IL-8 levels were elevated, but overexpression of miR-146b-5p partially reversed these changes.</p><p><strong>Conclusion: </strong>miR-146b-5p expression is down-regulated in the serum of SAP patients and cells, and it has a good diagnostic effect. It may be a potential biomarker and therapeutic target for AP.</p>","PeriodicalId":12862,"journal":{"name":"Hereditas","volume":"162 1","pages":"93"},"PeriodicalIF":2.7,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12126874/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HereditasPub Date : 2025-05-31DOI: 10.1186/s41065-025-00462-z
Yan-Zhen Wang, Jiang Yang, Xin-Min Han
{"title":"Clinical efficacy of Shaomazhijing granules in the treatment of Tourette's syndrome: a randomized controlled trial.","authors":"Yan-Zhen Wang, Jiang Yang, Xin-Min Han","doi":"10.1186/s41065-025-00462-z","DOIUrl":"10.1186/s41065-025-00462-z","url":null,"abstract":"<p><strong>Objective: </strong>We designed this study to verify the clinical efficacy and safety of Shaomazhijing granules, a Chinese patent medicine, in the treatment of Tourette's syndrome (TS) with liver-yang hyperactivity, liver wind, and phlegm-fire disturbance.</p><p><strong>Methods: </strong>We enrolled a total of 603 children and adolescents aged 5-18 years with TS in this randomized, double-blinded, multicenter study. We randomly assigned participants to a Shaomazhijing granules group, a Tiapride group, or a placebo group in a ratio of approximately 3:1:1, respectively. We evaluated the treatment results using the traditional Chinese medicine (TCM) syndrome quantitative classification scale and also compared the incidence of adverse events among the three groups.</p><p><strong>Results: </strong>The TCM syndrome of all patients improved over time. At week eight of TCM treatment, the overall syndrome score, primary symptoms (muscle tics), and secondary symptoms (emotional and psychological) of patients in the Shaomazhijing granules and tiapride groups showed significant improvements when compared to that of patients in the placebo group. Compared with the tiapride group, the Shaomazhijing granules group showed better improvement in the secondary symptoms (P < 0.05). While the clinical efficacy for primary symptoms of patients in the Shaomazhijing granules was similar (P = 0.969) with that of patients in tiapride groups. The TCM syndrome clinical control rate and the clinically excellent effectiveness rate of the Shaomazhijing granules group (3.45% and 44.51%) and tiapride group (2.86% and 26.67%) were higher than that of the patients in placebo group (1.04% and 12.50%, P < 0.001). Patients in placebo group (11.2%) and Shaomazhijing granules group (13.8%) had significantly lower overall adverse event rates in comparison with those in tiapride group (26.8%, P = 0.002).</p><p><strong>Conclusion: </strong>The clinical efficacy of Shaomazhijing granules is comparable to tiapride in reducing reducing the primary symptoms (muscle tics) of TS. Besides, it showed better efficacy in improving secondary (emotional and psychological) symptoms. Its safety profile is better than tiapride. Based on these results, Shaomazhijing granules can be considered a safe and effective treatment for patients with TS.</p>","PeriodicalId":12862,"journal":{"name":"Hereditas","volume":"162 1","pages":"90"},"PeriodicalIF":2.7,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125844/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HereditasPub Date : 2025-05-29DOI: 10.1186/s41065-025-00458-9
Tingting Zhao, Ying Peng
{"title":"Identification of immune-related genes and molecular subtypes associated with preeclampsia via bioinformatics analysis and experimental validation.","authors":"Tingting Zhao, Ying Peng","doi":"10.1186/s41065-025-00458-9","DOIUrl":"10.1186/s41065-025-00458-9","url":null,"abstract":"<p><strong>Background: </strong>Preeclampsia (PE) is a pregnancy disorder that occurs after 20 weeks of pregnancy. The objective of this study was to identify potential immune-related biomarkers and molecular subtypes for the treatment of PE.</p><p><strong>Methods: </strong>Three datasets of GSE10588, GSE25906 and GSE48424 were downloaded from the Gene Expression Omnibus (GEO) database. The names of immune-related genes were retrieved from the ImmPort immune database. To screen the differentially expressed immune-related genes, the \"limma\" R package was used. An analysis of logistic regression was used to identify the key genes and a nomogram was constructed using these key genes. These key gene expression profiles were further validated using qRT-PCR. In addition, the landscape of immune cell infiltration was investigated using the CIBERSORTX software. The potential molecular subtypes of PE were also investigated using the \"ConsensusClusterPlus\" R package.</p><p><strong>Results: </strong>The 103 immune-related genes differentially expressed were identified, including 47 up-regulated genes and 56 down-regulated genes. Univariate and multivariate logistic regression analysis was used to screen five key genes, including CCL24, ENG, LCP2, GNAI1 and FLT3. The key genes were strongly associated with immune cell infiltration. Two molecular subtypes (C1 and C2) were identified. Both exhibited distinct levels of immune cell infiltration and gene expression.</p><p><strong>Conclusion: </strong>This study identified five key genes, as well as immune-related subtypes, that could provide potential therapeutic targets and aid in the design of more precise PE immunotherapy.</p>","PeriodicalId":12862,"journal":{"name":"Hereditas","volume":"162 1","pages":"89"},"PeriodicalIF":2.7,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12123883/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}