Frontiers in Life Science最新文献_第9页

Antioxidant, antiproliferative, genotoxic and cytoprotective effects of the methanolic extract of Padina tetrastromatica on human breast adenocarcinoma and embryonic fibroblast cell lines 四斑马草甲醇提取物对人乳腺腺癌和胚胎成纤维细胞系的抗氧化、抗增殖、遗传毒性和细胞保护作用

Frontiers in Life Science Pub Date : 2015-06-09 DOI: 10.1080/21553769.2015.1051245

Y. Chia, M. Kanthimathi, J. Rajarajeswaran, K. S. Khoo, H. Cheng

{"title":"Antioxidant, antiproliferative, genotoxic and cytoprotective effects of the methanolic extract of Padina tetrastromatica on human breast adenocarcinoma and embryonic fibroblast cell lines","authors":"Y. Chia, M. Kanthimathi, J. Rajarajeswaran, K. S. Khoo, H. Cheng","doi":"10.1080/21553769.2015.1051245","DOIUrl":"https://doi.org/10.1080/21553769.2015.1051245","url":null,"abstract":"The in vitro antioxidant, antiproliferative, genotoxic and cytoprotective effects of the methanolic extract of the seaweed Padina tetrastromatica were assayed using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, superoxide, nitric oxide and hydroxyl radical scavenging assays, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and comet assays. The methanolic extract of P. tetrastromatica showed considerable antioxidant activity through inhibition of DPPH and hydroxyl radicals, with median inhibitory concentration (IC50) values of 45.57 ± 1.63 and 36.58 ± 2.13 µg/ml, respectively. Treatment of the human breast adenocarcinoma cell line MCF-7 with the methanolic extract, at a concentration range of 0–500 µg/ml, showed considerable antiproliferative effects, with an IC50 value of 125 ± 2.03 µg/ml. The genotoxic and cytoprotective effects of the extract on the human breast adenocarcinoma cell line MCF-7 and the embryonic fibroblast cell line 3T3-L1 were evaluated by the in vitro comet assay, and the extract demonstrated a genotoxic effect on MCF-7 and antigenotoxic and cytoprotective effects on 3T3-L1 cells. The methanolic extract of P. tetrastromatica was more effective in inducing a genotoxic effect in MCF-7 cells and a cytoprotective effect in 3T3-L1 cells, compared with the standard cancer chemotherapy drug doxorubicin, which induced greater DNA damage in the normal 3T3 cells.","PeriodicalId":12756,"journal":{"name":"Frontiers in Life Science","volume":"8 1","pages":"411 - 418"},"PeriodicalIF":0.0,"publicationDate":"2015-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21553769.2015.1051245","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60089736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Salmonella: A review on pathogenesis, epidemiology and antibiotic resistance 沙门氏菌:发病机制、流行病学及抗生素耐药性研究进展

Frontiers in Life Science Pub Date : 2015-06-09 DOI: 10.1080/21553769.2015.1051243

S. Eng, Priyia Pusparajah, Nurul-Syakima Ab Mutalib, Hooi‐Leng Ser, Kok-Gan Chan, Learn‐Han Lee

{"title":"Salmonella: A review on pathogenesis, epidemiology and antibiotic resistance","authors":"S. Eng, Priyia Pusparajah, Nurul-Syakima Ab Mutalib, Hooi‐Leng Ser, Kok-Gan Chan, Learn‐Han Lee","doi":"10.1080/21553769.2015.1051243","DOIUrl":"https://doi.org/10.1080/21553769.2015.1051243","url":null,"abstract":"Salmonella is one of the most frequently isolated foodborne pathogens. It is a major worldwide public health concern, accounting for 93.8 million foodborne illnesses and 155,000 deaths per year. To date, over 2500 Salmonella serotypes have been identified and more than half of them belong to Salmonella enterica subsp. enterica, which accounts for the majority of Salmonella infections in humans. Salmonella infections that involve invasive serotypes are often life threatening, necessitating appropriate and effective antibiotic therapy. The emergence of multi-drug-resistant (MDR) Salmonella serotypes is having a great impact on the efficacy of antibiotic treatment, and an increasing prevalence of MDR strains may lead to an increase in mortality rates of Salmonella infections. Epidemiological studies indicate that MDR Salmonella serotypes are more virulent than susceptible strains, as reflected by increased severity and more prolonged disease in patients infected by MDR strains. Preventive measures have been proposed to eliminate the spread of Salmonella infection. While the maintenance of effective food hygiene and water sanitation remain the cornerstones, additional measures such as restriction of indiscriminate use of antibiotics in food animals are important. This review provides an overview of Salmonella infection, and discusses the nomenclature, pathogenesis, clinical manifestations, epidemiology and antibiotic resistance of Salmonella.","PeriodicalId":12756,"journal":{"name":"Frontiers in Life Science","volume":"8 1","pages":"284 - 293"},"PeriodicalIF":0.0,"publicationDate":"2015-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21553769.2015.1051243","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60089663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 546

Effect of honey on purified equine myeloperoxidase activity and superoxide radical production in activated Polymorphonuclear neutrophils 蜂蜜对活化的多形核中性粒细胞中纯化马髓过氧化物酶活性和超氧化物自由基产生的影响

Frontiers in Life Science Pub Date : 2015-06-08 DOI: 10.1080/21553769.2015.1044131

Saâd Aissat, H. Benbarek, T. Franck, S. Kohnen, G. Deby-dupont, D. Serteyn, M. Ahmed, A. Mouithys-Mickalad

{"title":"Effect of honey on purified equine myeloperoxidase activity and superoxide radical production in activated Polymorphonuclear neutrophils","authors":"Saâd Aissat, H. Benbarek, T. Franck, S. Kohnen, G. Deby-dupont, D. Serteyn, M. Ahmed, A. Mouithys-Mickalad","doi":"10.1080/21553769.2015.1044131","DOIUrl":"https://doi.org/10.1080/21553769.2015.1044131","url":null,"abstract":"Three Algerian honey samples (nectar honey, mixed honey and honeydew honey) were analysed for their total phenolic content (TPC) using the Folin–Ciocalteu reagent and total flavonoid content (TFC) by the aluminium chloride method. The antioxidant activity was tested both against reactive oxygen species (ROS) produced by phorbol 12-myristate 13-acetate (PMA)-stimulated equine polymorphonuclear neutrophils (PMNs) and on purified equine myeloperoxidase (MPO) enzyme activity. The ROS production by stimulated PMNs was measured by lucigenin-enhanced chemiluminescence. Specific immunological extraction followed by enzymatic detection (SIEFED) was used to measure specifically the activity of equine MPO. Nectar honey, mixed honey and honeydew honey had TPCs of 59.52 ± 1.31, 68.36 ± 1.20 and 122.76 ± 4.20 mg gallic acid equivalents (GAE)/100 g honey, respectively (mean ± SD). As for the TPC, the highest TFC was observed for honeydew honey [20.55 ± 0.72 mg catechine equivalents (CE)/100 mg honey], followed by mixed honey (7.47 ± 0.07 CE/100 mg honey) and nectar honey (4.80 ± 0.08 CE/100 mg honey). All the tested honeys displayed a dose-dependent inhibitory effect on the oxidant activities of PMNs. By the SIEFED technique, it was shown that most of the honeys interact directly with MPO: the light honeys (nectar honey and mixed honey) were inhibitors of MPO activity, while the dark honey had less inhibiting effect and even behaved as an enhancer of MPO activity at high concentrations.","PeriodicalId":12756,"journal":{"name":"Frontiers in Life Science","volume":"8 1","pages":"379 - 386"},"PeriodicalIF":0.0,"publicationDate":"2015-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21553769.2015.1044131","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60089679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

In-vivo and in-vitro techniques used to investigate Alzheimer's disease 用于研究阿尔茨海默病的体内和体外技术

Frontiers in Life Science Pub Date : 2015-06-02 DOI: 10.1080/21553769.2015.1044129

Vishvanath Tiwari, Vandana Solanki, Monalisa Tiwari

引用次数: 7

Production of ethanol from alkali-pretreated sugarcane bagasse under the influence of different process parameters 碱预处理甘蔗渣在不同工艺参数影响下生产乙醇

Frontiers in Life Science Pub Date : 2015-05-29 DOI: 10.1080/21553769.2015.1044620

M. Nadeem, Muhammad Usman Aftab, M. Irfan, Mubashar Mushtaq, A. Qadir, Q. Syed

引用次数: 10

Role of common carp (Cyprinus carpio) in aquaculture production systems 鲤鱼在水产养殖生产系统中的作用

Frontiers in Life Science Pub Date : 2015-05-22 DOI: 10.1080/21553769.2015.1045629

Mohammad Mustafizur Rahman

{"title":"Role of common carp (Cyprinus carpio) in aquaculture production systems","authors":"Mohammad Mustafizur Rahman","doi":"10.1080/21553769.2015.1045629","DOIUrl":"https://doi.org/10.1080/21553769.2015.1045629","url":null,"abstract":"Common carp (Cyprinus carpio) is considered to be a very important aquaculture species in many Asian and some European countries. It affects the aerobic decomposition of organic matter and nutrient availability in the water column via bioturbation of benthic sediment during feeding on benthic organisms. If the density of common carp is not excessive, an increase in nutrient availability may enhance photosynthesis and plankton production, whereas if it is excessive, it causes dramatic ecological disruption at both the community and ecosystem levels by changing the abiotic properties of the water. Therefore, the density of common carp is a very important factor that has a great effect on the aquatic ecosystem. The critical density of common carp largely depends on its habitat. In polyculture ponds, water quality, natural food resources and fish growth are strongly affected when the density of common carp approaches more than about 1000 kg ha−1. The critical density can be doubled if artificial feed is supplied to the carp. When its preferred food is not sufficient, the common carp switches to less preferred food and changes its behaviour and feeding niche. These factors make common carp a potential candidate not only for monoculture but also for polyculture ponds. This article reviews the role of common carp on the aquatic ecosystem, and the production and behaviour of fish in aquaculture production systems.","PeriodicalId":12756,"journal":{"name":"Frontiers in Life Science","volume":"8 1","pages":"399 - 410"},"PeriodicalIF":0.0,"publicationDate":"2015-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21553769.2015.1045629","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60089990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 104

Antimicrobial activity and resistance to heavy metals and antibiotics of heterotrophic bacteria isolated from sediment and soil samples collected from two Antarctic islands1 南极两个岛屿沉积物和土壤样品中异养细菌的抗菌活性及对重金属和抗生素的耐药性[j]

Frontiers in Life Science Pub Date : 2015-05-22 DOI: 10.1080/21553769.2015.1044130

I. Tomova, M. Stoilova-Disheva, Irina Lazarkevich, E. Vasileva-Tonkova

{"title":"Antimicrobial activity and resistance to heavy metals and antibiotics of heterotrophic bacteria isolated from sediment and soil samples collected from two Antarctic islands1","authors":"I. Tomova, M. Stoilova-Disheva, Irina Lazarkevich, E. Vasileva-Tonkova","doi":"10.1080/21553769.2015.1044130","DOIUrl":"https://doi.org/10.1080/21553769.2015.1044130","url":null,"abstract":"In this study, 24 Antarctic bacteria, isolated from sediment and soil samples from Deception and Galindez Islands, were characterized for their antimicrobial activity and response to 13 antibiotics and seven heavy metals. Multiple antibiotic resistance was observed for 67% of sediment isolates and 92% of soil isolates, suggesting medium anthropogenic impact in these Antarctic regions. The results revealed a varying response of the Antarctic bacteria to the tested heavy metals. All isolates showed multiple metal resistance towards two to six heavy metals, with minimum inhibitory concentrations ranging from 1.0 mM to 23.2 mM. The majority of the strains in both groups were resistant to lead, nickel, copper and zinc. Plasmids were detected in 21% of isolates. A consortium of highly metal-resistant bacteria could be developed with potential application for biological treatment of wastewaters. Strains that are highly sensitive to cadmium would be promising for developing biosensors to detect this highly toxic heavy metal in environmental samples. All Antarctic bacteria were found to inhibit the growth of one to all eight of the tested indicator bacteria, and 87% inhibited the growth of one to all four of the yeast indicator cultures. Promising psychrotolerant strains were detected as a valuable source of new antimicrobial compounds.","PeriodicalId":12756,"journal":{"name":"Frontiers in Life Science","volume":"8 1","pages":"348 - 357"},"PeriodicalIF":0.0,"publicationDate":"2015-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21553769.2015.1044130","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60089601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 49

Ecological data prediction and visualization system 生态数据预测与可视化系统

Frontiers in Life Science Pub Date : 2015-05-15 DOI: 10.1080/21553769.2015.1041167

Sorayya Malek, Cham Hui, L. C. Fong, Mogeeb A. A. Mosleh, P. Milow, S. K. Dhillon, Sharifah M. Syed

{"title":"Ecological data prediction and visualization system","authors":"Sorayya Malek, Cham Hui, L. C. Fong, Mogeeb A. A. Mosleh, P. Milow, S. K. Dhillon, Sharifah M. Syed","doi":"10.1080/21553769.2015.1041167","DOIUrl":"https://doi.org/10.1080/21553769.2015.1041167","url":null,"abstract":"Temporal patterns in ecological data can be visualized and communicated effectively through graphical means. The aim of this study was to develop a data prediction and visualization system based on historical data and thematic map technology to visualize forecast temporal ecological changes. The visualization system consists of prediction and data visualization modules. The prediction module is developed using a hybrid evolutionary algorithm (HEA) to classify and predict noisy ecological data. The visualization module is developed using Dotnet Framework 2.0 to implement thematic cartography for volume visualization. The visualization system is evaluated by its capability in representing the output data on a map, and by predicting the abundance of Chlorophyta based on other water quality parameters. Rules for predicting Chlorophyta abundance had a success rate of almost 90%. The integration of computational data mining using HEA and visualization using thematic maps promises practical solutions and better techniques for forecasting temporal ecological changes, especially when data sets have complex relationships without clear distinction between various variables.","PeriodicalId":12756,"journal":{"name":"Frontiers in Life Science","volume":"8 1","pages":"387 - 398"},"PeriodicalIF":0.0,"publicationDate":"2015-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21553769.2015.1041167","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60089765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Chromosome and karyotype analysis of Hibiscus mutabilis f. mutabilis 变异芙蓉的染色体和核型分析

Frontiers in Life Science Pub Date : 2015-05-14 DOI: 10.1080/21553769.2015.1041166

Yu-ping Li, Xinlei Zhang, Wen-ting Wu, Shu-xing Miao, Jun-li Chang

引用次数: 13

The science of rabies in tropical regions: From epidemiological pandemonium to prevention 热带地区狂犬病科学:从流行病学大混乱到预防

Frontiers in Life Science Pub Date : 2015-05-13 DOI: 10.1080/21553769.2015.1041186

R. Gundamaraju, R. Vemuri, Lam Sau Kuen, R. Manikam, Rajeev K. Singla, S. Sekaran, Ramesh Chakrapani

{"title":"The science of rabies in tropical regions: From epidemiological pandemonium to prevention","authors":"R. Gundamaraju, R. Vemuri, Lam Sau Kuen, R. Manikam, Rajeev K. Singla, S. Sekaran, Ramesh Chakrapani","doi":"10.1080/21553769.2015.1041186","DOIUrl":"https://doi.org/10.1080/21553769.2015.1041186","url":null,"abstract":"Rabies is a devastating viral disease affecting the central nervous system. The fatality rate is very high compared to other viral diseases. Inadequate vaccination, lack of awareness, cost of medications and ineffective post-prophylactic surveillance are some of the major reasons for the high prevalence of rabies in tropical regions, leading to more than 40,000 cases annually presenting a major threat to public health. The present report aims to provide an overview of the lethal effects of rabies in major regions of the tropics, such as China and its neighboring countries, India, Bangkok, Thailand, all major regions of Africa, such as Tanzania and Ethiopia, and tropical regions of South America. The review is based on rich literature sources including epidemiological and endemic surveys, sample analysis, and health and hygiene reports from various parts of the world. The epidemiology is elucidated in the present article. The review also highlights the incidence of cases and aspects of prophylaxis. A bird's-eye view of rabies and its epidemic impact will encourage researchers to act further on the disease, which will lead to better awareness and treatment options.","PeriodicalId":12756,"journal":{"name":"Frontiers in Life Science","volume":"8 1","pages":"210 - 214"},"PeriodicalIF":0.0,"publicationDate":"2015-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21553769.2015.1041186","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60089876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2