Buletin Anatomi dan Fisiologi最新文献

{"title":"Histopatologis Aorta Tikus Putih Setelah Pemberian Teh Kombucha Konsentrasi 75% Berdasarkan Waktu Fermentasi","authors":"Sri Isdadiyanto, Stefanus Fajar","doi":"10.14710/baf.8.2.2023.130-137","DOIUrl":"https://doi.org/10.14710/baf.8.2.2023.130-137","url":null,"abstract":"Tujuan penelitian ini untuk mengetahui histopatologis aorta tikus putih (Rattus norvegicus) setelah pemberian teh kombucha kadar 75% dengan variasi waktu fermentasi. Penelitian ini menggunakan tikus putih (Rattus norvegicus) jantan sebanyak 16 ekor umur 2 bulan, dengan perlakuan Teh kombucha yang difermentasi selama 6, 9 dan 12 hari pada suhu 25oC per oral. Penelitian ini menggunakan Rancangan Acak Lengkap dengan 4 perlakuan  dan 4 ulangan, yaitu : P0 = kontrol, tanpa tambahan teh kombucha, P1 = air minum + 1,8 ml teh kombucha pagi dan sore fermentasi 6 hari, P2 = air minum + 1,8 ml teh kombucha pagi dan sore fermentasi 9 hari, P3 = air minum + 1,8 ml teh kombucha pagi dan sore fermentasi 12 hari. Penelitian dilakukan selama 28hari. Pada hari terakhir perlakuan, hewan dikorbankan dan diambil aorta serta jantung untuk pembuatan preparat histopatologi. Pengamatan preparat histopatologis aorta dengan pewarnaan hematoksilin-eosin dan dianalis secara deskriptif. Aorta tikus putih yang diberi teh kombucha yang difermentasi selama 6, 9 dan 12 hari menunjukkan gambaran normal. Berdasarkan hasil penelitian, maka dapat disimpulkan bahwa pemberian teh kombucha berdasarkan waktu fermentasi tidak mempengaruhi  struktur histologi aorta.The objective of this study was to analyze the aortic histology structure of rat (Rattus norvegicus) after treatment by 100% kombucha tea with variation of fermentation time. Sixteen male Rattus norvegicus, 2 months of age were used as experimental animals treated with kombucha tea that has been fermented for 6, 9 and 12 days at the temperature of 25oC per oral. The research design used to a completely randomized with 4 treatment (for 4 weeks) and 4 replications, namely: P0 = control, without of kombucha tea, P1 = water + 1,8 ml kombucha tea that has been fermented for 6 days in the morning and afternoon, P2 = water + 1,8 ml kombucha tea that has been fermented for 9 days in morning and afternoon, P3 = water + 1,8 ml kombucha tea that has been fermented for 12 days in morning and afternoon. The study was conducted for 28 days. On the last day of treatment, the animals were sacrificed and the aorta and heart were taken for making histopathological preparations. Observation of histopathological preparations of the aorta with hematoxylin-eosin staining and analyzed descriptively. Aorta of white rats fed with fermented kombucha tea for 6, 9 and 12 days showed normal appearance. Based on the results of the study, it can be concluded that administration of kombucha tea based on fermentation time does not affect the histological structure of the aorta.","PeriodicalId":127406,"journal":{"name":"Buletin Anatomi dan Fisiologi","volume":"121 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139229895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Struktur Histologi Testis Tikus Putih (Rattus Norvegicus L.) Setelah Pemberian Sediaan Nanokitosan Ekstrak Etanol Daun Mimba (Azadirachta Indica A.Juss)","authors":"Agung Janika Sitasiwi, Siti Muflichatun Mardiati, Alfriyan Krisna Melati","doi":"10.14710/baf.8.2.2023.122-129","DOIUrl":"https://doi.org/10.14710/baf.8.2.2023.122-129","url":null,"abstract":"Daun mimba (Azadirachta indica) memiliki banyak manfaat dalam bidang kesehatan. Hasil pengujian yang sudah dilakukan menunjukkan bahwa pemberian ekstrak daun mimba dapat mempengaruhi sistem reproduksi pada hewan jantan, namun efek yang diberikan tidak signifikan terhadap organ testis. Ukuran senyawa yang terlalu besar diduga tidak dapat menembus blood-tissue barrier sehingga perlu dikemas dalam formula dengan ukuran yang lebih kecil, berupa nanopartikel. Tujuan penelitian ini adalah mengetahui pengaruh sediaan nanokitosan ekstrak etanol daun mimba (SNEEDM) terhadap histologis testis tikus putih. Penelitian ini menggunakan 32 ekor tikus umur 2 bulan dibagi menjadi 4 perlakuan yaitu K- (aquades 2ml), K+ (larutan sediaan nanokitosan 2ml). P1 (diberi sediaan nanokitosan dan ekstrak dengan perbandingan 1:0.5), P2 (diberi sediaan nanokitosan dan ekstrak etnaol daun mimba dengan perbandingan 1:1). Hasil penelitian menunjukkan pemberian ekstrak etanol mimba dalam bentuk nanopartikel kitosan berpengaruh tidak nyata terhadap bobot testis, diameter tubulus seminiferus dan skor kerusakan tubulus seminiferus namun berpengaruh nyata terhadap ketebalan epitel tubulus seminiferus (P<0.05), sehingga dapat disimpulkan bahwa pemberian SNEEDM berpengaruh terhadap penurunan ketebalan lapisan epitel tubulus seminiferus. Azadirachta indica have many health benefits. The results of tests that have been carried out show that the administration of neem leaf extract can affect the reproductive system in male animals, but the effect given is not significant to the testicular organs. The size of the compound that is too large is thought to be unable to penetrate the blood-tissue barrier, so it could be packed in a small size particles. The purpose of this study was to determine the effect of neem leaf ethanol extract nanocytosan preparation (SNEEDM) on the histology of white rat testes. This study used 32 rats aged 2 months divided into 4 treatments namely K- (2ml distilled water), K+ (treated by 2ml preparation of nanochitosan solution), P1 (treated by preparation of nanochitosan and neem leaf ethanol extract 1:0.5 in proportion), P2 (treated by preparation of nanochitosan and neem leaf ethanol extract with 1:1 in proportion). The results are the administration of neem ethanol extract in the form of chitosan nanoparticles had no significant effect on testicular weight, seminiferous tubule diameter and seminiferous tubule damage score but had a significant effect on seminiferous tubule epithelial thickness (P <0.05), so it can be concluded that the administration of SNEEDM has an effect on reducing the thickness of the seminiferous tubule epithelial layer.","PeriodicalId":127406,"journal":{"name":"Buletin Anatomi dan Fisiologi","volume":"520 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139263008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aterosklerosis pada Tikus Putih (Rattus norvegicus L.) yang Diinduksi dengan Insulin Eksogen","authors":"Teguh Suprihatin, Widya Millatina Fazwah, Silvana Tana","doi":"10.14710/baf.8.2.2023.114-121","DOIUrl":"https://doi.org/10.14710/baf.8.2.2023.114-121","url":null,"abstract":"Insulin memiliki peran mendasar dalam pengendalian proses seluler dan fisiologis. Paparan berlebih insulin menyebabkan resistensi insulin, hiperinsulinemia, dan hiperglikemia. Penggunaan insulin pada pasien penderita DM rentan menyebabkan terjadinya  resistensi insulin yang bisa memperparah kondisi pasien.Tujuan penelitian ini adalah menganalisis pengaruh pemberian injeksi insulin eksogen terhadap tikus wistar (Rattus norvegicus L. Penelitian ini merupakan penelitian eksperimental dengan Rancangan Acak Lengkap (RAL), menggunakan 21 ekor Rattus norvegicus L. jantan berumur 21 hari yang dibagi dalam 3 kelompok perlakuan dengan 7 kali ulangan. P0 merupakan kontrol, P1 diinjeksi insulin eksogen 1,80 IU/200gBB setiap hari selama 14 hari, dan P2 diinjeksi insulin eksogen 1,80 IU/200gBB setiap hari selama 28 hari. Perlakuan diberikan selama 1 bulan. Pembedahan dilakukan setelah perlakuan berakhir untuk pembuatan preparat, kemudian dilanjutkan dengan pengukuran diameter dan lumen dari aorta dan arteri coronaria, serta ketebalan dinding aorta dan arteri coronaria. Data penelitian dianalisis menggunakan analysis of variance (ANOVA) pada taraf kepercayaan 95%. Hasil penelitian menunjukkan bahwa terdapat perbedaan tidak nyata (P>0.05) pada lebar diameter arteri coronaria dan aorta, lebar lumen arteri coronaria dan aorta, ketebalan tunika intima, media dan adventisia arteri coronaria dan aorta serta ukuran sel otot jantung. Berdasarkan hasil penelitian yang diperoleh dapat disimpulkan bahwa injeksi insulin eksogen tidak menimbulkan penebalan aterosklerosis pada Rattus norvegicus L.  Insulin has a fundamental role in control of cellular and physiological processes. Overexposure to insulin causes insulin resistance, hyperinsulinemia, and hyperglycemia. This study was to analyze the effect of exogenous insulin injection to white rats (Rattus norvegicus L.). This is an experimental study with a completely randomized design, using 21 male Rattus norvegicus L. 21 days old which were divided into 3 treatment groups with 7 replications. P0 is the control groups, P1 is exogenous insulin injection 1.80 IU/200gBW/day for 14 days, and P2 is exogenous insulin injection 1.80 IU/200gBW/day for 28 days. Treatment was given for 1 month. Surgery was carried out after the treatment ended and preparations were made, then continued with measuring the diameter and the width of the lumen of aorta and coronary arteries. The thickness of the aortic and coronary arteries’s walls. Data were analyzed using ANOVA at the 95% confidence level. The results showed that there was no significant difference (P>0.05) based on the results, exogenous insulin injection can not trigger atherosclerosis in white rats.","PeriodicalId":127406,"journal":{"name":"Buletin Anatomi dan Fisiologi","volume":"49 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139277656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pertambahan Bobot Tubuh, Panjang Tubuh dan Tinggi Tubuh Ikan Nila Merah (Oreochromis niloticus) yang Dipelihara Pada Aerasi dan Padat Tebar Berbeda","authors":"M. Djaelani, Kasiyati Kasiyati, S. Sunarno","doi":"10.14710/baf.8.2.2023.106-113","DOIUrl":"https://doi.org/10.14710/baf.8.2.2023.106-113","url":null,"abstract":"Penelitian ini bertujuan untuk mengetahui pengaruh aerasi dan padat tebar terhadap pertambahan bobot tubuh, panjang tubuh dan tinggi tubuh ikan nila merah (Oreochromis niloticus).  Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) faktorial dengan dua perlakuan, terdiri atas perlakuan kelompok dilengkapi dengan satu aerator dan perlakuan kelompok dilengkapi dengan dua aerator serta tiga tingkatan padat tebar 2,4,8 ekor ikan. Pada padat tebar normal sebanyak 2 ekor ikan nila merah dengan berat 17±1,6 g dipelihara dalam kontainer boks kapasitas 40 liter yang diisi air sebanyak 34 liter. Ikan dipelihara selama 2 bulan. Variabel yang diamati pada penelitian ini adalah pertambahan bobot tubuh, panjang tubuh dan tinggi tubuh ikan nila. Faktor lingkungan yang diamati pada penelitian ini meliputi Oksigen terlarut (DO), pH, suhu, kandungan ammonia, nitrit dan nitrat. Hasil penelitian ini menunjukkan bahwa pemeliharaan ikan nila merah dengan penambahan aerator berpengaruh nyata (P<0,05) terhadap pertambahan bobot tubuh, panjang tubuh dan tinggi tubuh ikan nila merah. This study aims to determine the effect of aeration and stocking density on body weight gain, body length and body height of red tilapia (Oreochromis niloticus). This study used a factorial completely randomized design (CRD) with two treatments, consisting of a group treatment equipped with one aerator and a group treatment equipped with two aerators and three levels of stocking density of 2,4,8 fish. At normal stocking densities, 2 red tilapia weighing 17 ± 1.6 g were reared in a 40 liter capacity box filled with 34 liters of water. Fish kept for 2 months. The variables observed in this study were the increase in body weight, body length and body height of tilapia fish. Environmental factors observed in this study include dissolved oxygen (DO), pH, temperature, ammonia, nitrite and nitrate content. The results of this study indicated that rearing red tilapia with the addition of an aerator had a significant (P<0.05) effect on body weight gain, body length and body height of red tilapia.","PeriodicalId":127406,"journal":{"name":"Buletin Anatomi dan Fisiologi","volume":"155 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139342301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efek Interaktif Selenium dan Kromium Terhadap Pertumbuhan Vegetatif dan Generatif Tagetes erecta L.","authors":"Roslinda Kristin Tambunan, Sri Kasmiyati","doi":"10.14710/baf.8.2.2023.96-105","DOIUrl":"https://doi.org/10.14710/baf.8.2.2023.96-105","url":null,"abstract":"Ffek toksik kromium (Cr) pada tanaman menyebabkan terjadinya perubahan pertumbuhan. Penggunaan unsur mikronutrien selenium merupakan salah satu strategi yang efektif untuk menurunkan efek toksik logam berat termasuk Cr dan mengatur pertumbuhan serta perkembangan tanaman. Tujuan penelitian ini menganalisis efek interaktif selenium dan kromium terhadap pertumbuhan vegetatif dan generatif Tagetes erecta.  Efek interaktif selenium dan kromium dikaji secara eksperimental menggunakan rancangan acak lengkap (RAL) dengan 2 faktor yaitu konsentrasi ion kromat (CrO42-) dalam bentuk K2CrO4 dengan 3 level konsentrasi (0, 50 dan 100 mg//L), dan konsentrasi selenium dalam bentuk selenat (SeO42-) dengan 3 level konsentrasi (0 μM/L, 40 μM/L, dan 80 μM/L). Interaksi selenium dan kromium mempengaruhi secara nyata pertumbuhan vegetatif dan generatif T. erecta. Penambahan selenium 80 μM/L mampu meningkatkan tinggi tanaman, jumlah daun, panjang akar dan jumlah bunga berturut-turut 8,5%, 11,5%, 4,5%, dan 6,0% dibandingkan tanaman kontrol (tanpa Se) pada tanaman dengan perlakuan Cr 50 mg/L. Pada penambahan Se sebesar 40 dan 80 μM/L, T. erecta dengan perlakuan Cr 100 mg/L terjadi penurunan tinggi tanaman dan panjang akar sebesar 5,4-5,9% dan 2,6-3,3% dibanding tanaman kontrol. Penambahan Se 40 dan 80 μM/L pada tanaman dengan perlakuan Cr 100 mg/L meningkatkan jumlah daun dan jumlah bunga secara nyata dibanding tanaman kontrol. Penelitian yang mengkaji tentang dosis dan peran Se dalam mekanisme toleransi terhadap logam Cr masih perlu dilakukan.   The toxic effects of chromium (Cr) on plants is to cause growth changes. The use of the micronutrient selenium is an effective strategy to reduce the toxic effects of heavy metals including Cr and regulate plant growth and development. The aim of this study was to analyze the interactive effects of selenium and chromium on the vegetative and generative growth of Tagetes erecta. The interactive effect of selenium and chromium was studied experimentally using a completely randomized design (CRD) with 2 factors, namely the concentration of chromate ion (CrO42-) in the form of K2CrO4 with 3 concentration levels (0, 50 and 100 mg//L), and the concentration of selenium in the form of selenat (SeO42-) with 3 concentration levels (0 μM/L, 40 μM/L, and 80 μM/L). The interaction of selenium and chromium significantly affects the vegetative and generative growth of T. erecta. The addition of 80 µM/L selenium was able to increase plant height, number of leaves, root length and number of flowers 8.5%, 11.5%, 4.5% and 6.0% compared to control plants (without Se), on plants treated with Cr 50 mg/L. The addition of Se of 40 and 80 μM/L, T. erecta with Cr 100 mg/L treatment reduced plant height and root length by 5.4-5.9% and 2.6-3.3% compared to control plants. In contrast, the addition of Se 40 and 80 μM/L to plants treated with Cr 100 mg/L significantly increased the number of leaves and the number of flowers compared to the control plants. R","PeriodicalId":127406,"journal":{"name":"Buletin Anatomi dan Fisiologi","volume":"207 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139345452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pengaruh Kolkisin Metode Semprot dan Tetes terhadap Respon Fenotipik dan Profil Kromosom Phalaenopsis pulcherrima","authors":"Nuri Lailatul Istiqomah, Tintrim Rahayu, Gatra Ervi Jayanti","doi":"10.14710/baf.8.2.2023.86-95","DOIUrl":"https://doi.org/10.14710/baf.8.2.2023.86-95","url":null,"abstract":"Kolkisin  merupakan mutagen untuk memengaruhi karakter  Phalaenopsis pulcherrima  tipe bunga kecil, diharapkan bunga yang lebih besar saat fase generatif. Tujuan penelitian adalah mengamati pengaruh terbaik melalui metode dan konsentrasi kolkisin yang berbeda terhadap Phalaenopsis pulcherrima pada fase vegetatif. Penelitian dilakukan di  Laboratorium Orchidology dan Nursery, Universitas Islam Malang. Metode penelitian yang digunakan adalah metode eksperimen. Rancangan penelitian berupa rancangan Acak Lengkap (RAL) dengan tiga ulangan dan terdiri dari perlakuan tetes, semprot, dan kombinasi (tetes dan semprot). Konsentrasi kolkisin 0,5%, 1%, 1,5% dan 2%. Hasil uji multivariate dan uji univariat menunjukkan metode dan konsentrasi kolkisin yang berbeda berpengaruh pada respon fenotipik Phalaenopsis pulcherrima (diameter batang, diameter akar, luas daun, ketebalan daun serta berat basah tanaman). Pada uji lanjut Games-Howell 5% perlakuan tetes dengan konsentrasi 1% merupakan perlakuan kolkisin terbaik berdasarkan indikator diameter batang, luas daun serta berat tanaman dan perlakuan tetes dengan konsentrasi kolkisin 0,5% untuk indikator ketebalan daun. Perlakuan terbaik adalah tetes dan kombinasi (tetes dan semprot) dengan konsentrasi 2% terhadap diameter akar Phalaenopsis pulcherrima.   Colchicine is a mutagen to the affect character of Phalaenopsis pulcherrima’s small flower can be bigger flowers for the generative phase. The research was to observe the best effect of different methods and concentrations of colchicine on Phalaenopsis pulcherrima in the vegetative phase. The research was conducted in the Orchidology and Nursery Laboratory, Universitas Islam Malang. The experimental method is a method of this research. Research Design with completely randomized design (CRD) in three replications of drip, spray, and combination (drops and sprays) with concentrations of 0.5%, 1%, 1.5% and 2% of colchicine’s treatment. The multivariate and  univariate test showed different methods and concentrations of colchicine effected on phenotypic response of Phalaenopsis pulcherrima (stem diameter, root diameter, leaf area, leaf thickness, and plant weight). In Games-Howell test 5%, drip treatment and 1% concentration was the best colchicine treatment in indicators of stem diameter, leaf area, plant weight and 0.5% concentration in leaf thickness. Drops and combinations treatment (concentration of 2%) is best treatment of root diameter. The best treatments are drops and combinations (drops and sprays) with a concentration of 2% on the root diameter of Phalaenopsis pulcherrima.","PeriodicalId":127406,"journal":{"name":"Buletin Anatomi dan Fisiologi","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139369436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laju Pertumbuhan Puyuh Jepang (Coturnix Coturnix Japonica) Setelah Diberi Serbuk Kunyit Dan Kurkumin Dalam Air Minum)","authors":"Silvana Tana, Abigael Eflen Sraun, T. Saraswati","doi":"10.14710/baf.8.1.2023.79-85","DOIUrl":"https://doi.org/10.14710/baf.8.1.2023.79-85","url":null,"abstract":"Penelitian ini bertujuan untuk mengetahui potensi serbuk kunyit dan kurkumin yang diberi dalam minum untuk meningkatkan laju pertumbuhan puyuh. Penelitian ini menggunakan 24 ekor puyuh jepang betina yang terdiri dari 3 perlakuan dan 8 ulangan, yaitu P0: sebagai kontrol, kelompok yang diberi air biasa, P1: kelompok puyuh diberi dosis kurkumin 0.15 mg/ekor/hari dan P2: kelompok puyuh diberi dosis bubuk kunyit 108 mg/ekor/hari. Parameter utama yang diamati adalah pertambahan bobot badan yang diukur setiap seminggu sekali pada masing-masing kelompok perlakuan, sedangkan parameter pendukung adalah konsumsi pakan, konsumsi minum dan konversi pakan.Pengukuran bobot dilakukan dengan timbangan pada setiap individu puyuh.Laju pertumbuhan diperoleh dengan menghitung selisih bobot minggu terakhir dengan bobot minggu pertamaselama 8 minggu. Data yang diperoleh dimasukkan dan diolah di Microsoft Exel 2010, mencari nilai rata-rata menggunakan fungsi AVERAGEdi Microsoft Exel.Hasil penelitian menunjukkan nilai rata-rata konsumsi pakan, bobot badan, laju pertumbuhan dan konversi pakan tidak mengalami perubahan setelah penambahan serbuk kunyit dan kurkumin, namun meningkatkan konsumsi minum puyuh. Kesimpulan dari penelitian ini adalah serbuk kunyit dan kurkumin tidak mempengaruhi konsumsi pakan, konsumsi minum, bobot dan konversi pakan sehingga menghambat laju pertumbuhan puyuh. This study aims to determine the effect of tumeric powder and curcumin dissolved in drinking water on the growth rate of Japanese quail. This study used 24 female quail consisting of 3 treatments and 8 replications, namely P0: as a control, the group was given plain water, P1: the quail group was given a dose of curcumin 0,15 mg / quail / day and P2: the quail group was given a dose of turmeric powder 108 mg / quail / day. The main parameters observed were body weight gain which was measured once a week in each treatment group, while the supporting parameters were feed consumption and drinking consumption, feed conversion. The weight measurement by weighing each quail individual. The growth rate is obtained by calculating the difference between the weight of the last week and the weight of the first week for 8 weeks. The data obtained from this study were processed using Microsoft Excel 2010 using the AVERAGE function. The results showed that the average value of feed consumption, body weight, growth rate and feed conversion after adding turmeric powder and curcumin to the treatment was significantly different from the control, but not increased drinking consumption of quail. The conclusion of this study is that the doses of turmeric powder and curcumin given has not had the potential to affect the growth rate of quails. The conclusion of this study was that the doses of curcumin 0,15 mg/quail/day and turmeric powder 108 mg/quail/day given to Japanese Quail had no potential to affect growth rate. ","PeriodicalId":127406,"journal":{"name":"Buletin Anatomi dan Fisiologi","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121370907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potensi Mangrove Avicennia Marina (Forsk.) sebagai Agen Fitoremidiasi Kadmium (Cd) di Tambak dan Laut Mangunharjo, Kecamatan Tugu, Kota Semarang","authors":"E. Hastuti, Fina Irodatul Afiyah, Munifatul Izzati","doi":"10.14710/baf.8.1.2023.71-78","DOIUrl":"https://doi.org/10.14710/baf.8.1.2023.71-78","url":null,"abstract":"Aktivitas industri di perairan Mangunharjo menghasilkan limbah yang dapat menyebabkan pencemaran logam berat salah satunya yaitu kadmium (Cd). Mangrove Avicennia marina dapat mengabsorbsi Cd dari sedimen dan air melalui akar kemudian di translokasi ke organ lain seperti daun dan akar sehingga berpotensi sebagai fitoremidiasi. Penelitian ini bertujuan untuk menganalisis kemampuan akumulasi Cd pada akar dan daun mangrove Avicennia marina di perairan laut dan tambak Mangunharjo. Penelitian ini menggunakan RAL dengan 2 faktor yaitu lokasi (tambak dan laut) dan organ (daun dan akar) masing-masing perlakuan dengan 3 ulangan. Analisis kandungan Cd menggunakan metode AAS (Atomic Absorption Spechtophotometry), data diuji dengan ANOVA, dilanjutkan dengan DMRT pada taraf uji 5 %. Hasil penelitian menunjukkan bahwa di tambak kadar Cd pada sedimen yaitu 1.0897 ppm dan pada air 0.0123 ppm, sedangkan di laut kadar Cd pada sedimen yaitu 0.1193 ppm dan pada air 0.0163 ppm. Faktor organ mangrove dan lokasi menujukkan interaksi terhadap kadar Cd dan nilai BCF. Kadar Cd tertinggi terdapat pada organ daun di tambak (0.2269 ppm) dan nilai terendah terdapat pada organ daun di laut (0.0200 ppm). Nilai BCF tertinggi terdapat pada organ akar di laut (0.5133) dan yang paling rendah adalah organ daun di laut (0.0567). Industrial activities in the waters of Mangunharjo produce waste that can cause heavy metal pollution, one of which is cadmium (Cd). Avicennia marina mangroves can absorb Cd from sediment and water through the roots and then translocated to other organs such as leaves and roots so that it has the potential as phytoremediation. This study aimed to analyze the ability of Cd accumulation in the roots and leaves of Avicennia marina mangroves in Ponds and Mangunharjo Seas. This study used RAL with 2 factors, namely location (ponds and sea) and organs (leaves and roots) each treatment with 3 replications. Analysis of Cd content using AAS (Atomic Absorption Spechtophotometry) method, data was tested by ANOVA, followed by DMRT at the 5% test level. The results showed that in ponds the Cd content in sediment was 1.0897 ppm and in water was 0.0123 ppm, while at sea the Cd level in sediment was 0.1193 ppm and in water was 0.0163 ppm. Mangrove organ factors and location showed an interaction with Cd levels and BCF values. The highest Cd levels were found in leaf organs in ponds (0.2269 ppm) and the lowest values were found in leaf organs in the sea (0.0200 ppm). The highest BCF value was found in root organs in the sea (0.5133) and the lowest was in leaf organs in the sea (0.0567). ","PeriodicalId":127406,"journal":{"name":"Buletin Anatomi dan Fisiologi","volume":"417 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"113986689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"UJi Kualitatif Metabolit Sekunder pada Beberapa Tanaman yang Berkhasiat sebagai Obat Tradisional","authors":"Abdul Khafid, Muhammad Dwijunianto Wiraputra, Agita Christyaji Putra, Nafiah Khoirunnisa, Aurora Awalia Kirana Putri, Sri Widodo Agung Suedy, Yulita Nurchayati","doi":"10.14710/baf.8.1.2023.61-70","DOIUrl":"https://doi.org/10.14710/baf.8.1.2023.61-70","url":null,"abstract":"Indonesia sebagai negara yang memiliki megabiodiversitas terbesar kedua saat ini memberikan manfaat yang begitu luar biasa. Diantara banyak tumbuhan yang ada, tidak sedikit pula tumbuhan yang bisa dimanfaatkan sebagai obat-obatan karena senyawa metabolit sekunder yang terkandung pada berbagai organnya, salah satunya adalah pada bagian daun. Beberapa senyawa metabolit sekunder memiliki khasiat obat, seperti flavonoid, saponin, alkaloid, steroid, triterpenoid dan tanin. Penelitian ini bertujuan untuk mengetahui senyawa metabolit sekunder yang terkandung di dalam daun beberapa tumbuhan berkhasiat obat, antara lain Salam (Syzygium polyanthum (Wight) Walp.), Salam Koja (Murraya koenigii), Sirih (Piper betle Linn), Pepaya (Carica papaya L.), dan Jeruk Purut (Citrus hystrixD. C.). Penelitian dilaksanakan dengan melakukan uji identifikasi senyawa metabolit sekunder pada eksrak maserasi beberapa sampel daun dalam pelarut etanol 70%. Hasil penelitian menunjukkan pada uji flavonoid, saponin, alkaloid, dan tanin positif pada kelima sampel daun, uji triterpenoid positif pada daun Jeruk Purut, uji steroid positif pada semua sampel kecuali daun Jeruk Purut. Indonesia as a country that has the second-largest mega biodiversity currently provides extraordinary benefits. Among the many plants that exist, there are so many plants that can be used as medicines because of secondary metabolites contained in various organs, one of which is in the leaves. Some of the secondary metabolic compounds had medicinal properties, such as flavonoids, saponins, alkaloids, steroids, triterpenoids, and tannins. This study aims to determine the secondary metabolite compounds contained in leaves of several plants with medicinal benefits, such as Bay Leaves (Syzygium polyanthum (Wight) Walp.), Curry Tree (Murraya koenigii), Betel (Piper betle Linn), Papaya (Carica papaya L.) and Kaffir lime  (Citrus hystrix DC). The research was done by identification test of secondary metabolites in the macerated extract of several leaf samples in 70% ethanol solvent. The results showed a positive flavonoid, saponin, alkaloid and tanin on all five-leaf samples, positive triterpenoid test on kaffir lime leaves, positive steroid test on all samples except kaffir lime leaves. ","PeriodicalId":127406,"journal":{"name":"Buletin Anatomi dan Fisiologi","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122674073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efek Pemberian Ekstrak Etanol Daun Mimba (Azadirachta indica A. Juss) Terhadap Struktur Histopatologi Hepar Tikus Putih (Rattus norvegicus L.) Jantan Hiperglikemia","authors":"Arthi Ridho Wicaksono, Siti Muflichatun Mardiati, Sri Isdadiyanto","doi":"10.14710/baf.8.1.2023.53-60","DOIUrl":"https://doi.org/10.14710/baf.8.1.2023.53-60","url":null,"abstract":"Hiperglikemia merupakan kondisi medis yang diindikasikan dengan tingginya kadar glukosa darah. Hiperglikemia dapat menyebabkan kondisi diabetes yang dapat merusak struktur histologis jaringan hepar. Mimba (Azadirachta indica A.Juss) merupakan tumbuhan obat yang dikenal di berbagai daerah di Indonesia. Ekstrak etanol daun mimba mengandung antioksidan flavonoid yang dapat membantu dalam perbaikan jaringan hepar yang rusak. Penelitian ini bertujuan untuk menganalisis efek ekstrak etanol daun mimba dalam perbaikan struktur histologis hepar. Penelitian ini menggunakan desain Rancangan Acak Lengkap (RAL). Tikus putih (Rattus norvegicus L.) sebanyak 18 ekor dibagi menjadi 6 kelompok perlakuan dan 3 kali perulangan. Penelitian dilaksanakan selama 27 hari. P0 (kontrol normal) tikus kondisi normal diberi akuades. P1 (kontrol negatif) tikus kondisi hiperglikemi diberi akuades. P2 (kontrol positif) tikus kondisi hiperglikemi diberi glibenklamid dosis 2.25 mg/kg BB. P3, P4, dan P5 merupakan kelompok tikus hiperglikemi yang diberi perlakuan ekstrak etanol daun mimba dengan dosis masing-masing 100, 200, dan 400 mg/kg BB. Kondisi hiperglikemi tikus diinduksi dengan pemberian aloksan dengan dosis 120 mg/kgBw. Data penelitian dianalisis menggunakan uji Anova pada signifikansi 95%, sedangkan data yang tidak terdistribusi secara normal dianalisis dengan uji Kruskal-Wallis. Hasil menunjukkan bahwa pemberian ekstrak etanol daun mimba berpengaruh nyata pada parameter diameter hepatosit dan skala kerusakan hepatosit (P<0,05), namun tidak memberikan pengaruh nyata pada parameter hepatosomatik indeks (P>0,05). Kesimpulan penelitian ini adalah pemberian ekstrak etanol daun mimba (Azadirachta indica A.Juss) dosis 400mg/KgBB efektif melindungi jaringan hepar tikus putih jantan dari kondisi hiperglikemia akibat induksi aloksan  Hyperglycemia is a medical condition indicated by the abnormally high blood sugar level in one’s body. Hyperglycemia usually leads to diabetes which is known to be capable of damaging the histological structures of liver tissues. Neem (Azadirachta indica A.Juss) is a well-known medicinal plant in various regions. Ethanolic extract of neem leaves contains the antioxidant flavonoid that can help repair damaged liver tissues. The aim of this research was to analyze the healing properties of the neem leaves ethanol extracts to the damaged liver tissues. This research used the Completely Randomized Design (CRD) model with a total of 18 male white rats which were divided into 6 treatment groups and 3 iterations. The research was carried out for 27 days. P0 (Normal control) the group of rats in normal condition were given distilled water. P1 (negative control) the group of rats in hyperglycemic condition were given distilled water. P2 (Positive control) the group of rats in hyperglycemic condition were given glibenclamide with the dose of 2.25 mg/kg Body Weight. P3, P4, and P5 were the group of rats in hyperglycemic conditions who were given neem leaves ethan","PeriodicalId":127406,"journal":{"name":"Buletin Anatomi dan Fisiologi","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126288597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}