GMS Hygiene and Infection Control最新文献

Evaluation of epidemiological, clinical, and microbiological features of vulvovaginal candidiasis. 外阴阴道念珠菌病的流行病学、临床和微生物学特征评价。

IF 1.7

GMS Hygiene and Infection Control Pub Date : 2025-04-30 eCollection Date: 2025-01-01 DOI: 10.3205/dgkh000544

Reza Faraji, Abbas Maleki, Abbas Gheisoori, Taha Rashidi, Amirhossein Salimi Mansouri, Fatemeh Rashidi, Sadegh Faraji, Alireza Kashefizadeh, Arezoo Bozorgomid

{"title":"Evaluation of epidemiological, clinical, and microbiological features of vulvovaginal candidiasis.","authors":"Reza Faraji, Abbas Maleki, Abbas Gheisoori, Taha Rashidi, Amirhossein Salimi Mansouri, Fatemeh Rashidi, Sadegh Faraji, Alireza Kashefizadeh, Arezoo Bozorgomid","doi":"10.3205/dgkh000544","DOIUrl":"10.3205/dgkh000544","url":null,"abstract":"Background: Vaginal candidiasis is induced by abnormal growth of yeast on the mucous membranes of the female genital tract. Approximately 75% of women experience a yeast infection once in their lifetime. This study explored the epidemiological, clinical, and microbiological features of vaginal candidiasis in diabetic women referred to health and treatment centers in Kermanshah in 2023.Methods: This cross-sectional descriptive study was conducted on 215 diabetic women. A questionnaire was prepared for each participant. The samples were examined microscopically and cultured on Sabouraud dextrose agar (SDA). To identify different species of Candida (C.), various complementary tests were performed, such as the germ tube and differential sugar absorption test (API). A sensitivity test was applied to positive samples by the broth macrodilution method. Data were analyzed using the chi-squared test in SPSS.Results: Out of the 215 vaginal swabs investigated, 66 specimens were Candida-species positive (30.7%). 11.6% of participants were diagnosed with candidal vulvovaginitis by direct microscopic examination and 20.9% by culturing on SDA. The Candida species isolated were: C. albi ca n s with 36 cases (54.5%), C. glabrata with 14 cases (21.2%), C. tropicalis with 9 cases (13.6%) and C. parapsilosis with 7 cases (10.6%). All species isolated showed the same sensitivity to the antifungal drugs used.Conclusion: The culture method was more sensitive than the direct microscopic examination. C. albicans was the most prevalent species isolated from patients. Non-albicans species were not prevalent.","PeriodicalId":12738,"journal":{"name":"GMS Hygiene and Infection Control","volume":"20 ","pages":"Doc15"},"PeriodicalIF":1.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101135/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the implementation of infection control policies in health facilities in the Popokabaka health district in the Democratic Republic of Congo. 评价刚果民主共和国波波卡巴卡卫生区卫生设施感染控制政策的执行情况。

IF 1.7

GMS Hygiene and Infection Control Pub Date : 2025-04-30 eCollection Date: 2025-01-01 DOI: 10.3205/dgkh000542

Fraste Kaswij Muswiya, Martin Mutuza Bakuzeza, Dalau Nkamba Mukadi

{"title":"Evaluation of the implementation of infection control policies in health facilities in the Popokabaka health district in the Democratic Republic of Congo.","authors":"Fraste Kaswij Muswiya, Martin Mutuza Bakuzeza, Dalau Nkamba Mukadi","doi":"10.3205/dgkh000542","DOIUrl":"10.3205/dgkh000542","url":null,"abstract":"Background: Healthcare quality in health facilities relies on the implementation of and providers' adherence to an effective infection control program. The aim of this study was to assess the implementation level of infection prevention and control (IPC) guidelines in healthcare facilities in a low-income country.Methods: This was a cross-sectional study conducted in 18 healthcare facilities of the Popokabaka health district in the Democratic Republic of Congo. Data were collected and analyzed following the IPC assessment framework developed by the World Health Organization (WHO). The framework consisted of eight different sections, of which each is scored up to 100. The level of implementation in each facility was assessed based on a scoring system: inadequate (0-200), basic (201-400), intermediate (401-600), and advanced (601-800).Results: The median score of all facilities was 181.3, IQR 145.0-228.1, with a range from inadequate to basic. Ten (55.6%) healthcare facilities had an inadequate IPC implementation level, while eight (44.4%) had a basic level. IPC education and training were the components that were the most poorly implemented in the surveyed facilities. None of these facilities had multimodal strategies to implement IPC interventions.Conclusion: The level of IPC guideline implementation in healthcare facilities of the Popokabaka health district remains basic as a result of low resource investment in the IPC program. It negatively impacts the quality of care and exposes patients and healthcare providers to healthcare-associated infections.","PeriodicalId":12738,"journal":{"name":"GMS Hygiene and Infection Control","volume":"20 ","pages":"Doc13"},"PeriodicalIF":1.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101134/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design and development of primers for detection of Streptococcus pneumoniae, Haemophilus influenzae, and Neisseria meningitidis. 设计和开发用于检测肺炎链球菌、流感嗜血杆菌和脑膜炎奈瑟菌的引物。

IF 1.7

GMS Hygiene and Infection Control Pub Date : 2025-04-30 eCollection Date: 2025-01-01 DOI: 10.3205/dgkh000545

Leila Azimi, Fatemeh Shirkavand, Shahnaz Armin, Fereshteh Karbasian, Hannan Khodaei

{"title":"Design and development of primers for detection of Streptococcus pneumoniae, Haemophilus influenzae, and Neisseria meningitidis.","authors":"Leila Azimi, Fatemeh Shirkavand, Shahnaz Armin, Fereshteh Karbasian, Hannan Khodaei","doi":"10.3205/dgkh000545","DOIUrl":"10.3205/dgkh000545","url":null,"abstract":"Background: The mortality rate of meningitis is still alarmingly high in certain regions across the globe. The objective of this research is to identify the most effective primers for detecting Streptococcus (S.) pneumoniae, Haemophilus (H.) influenzae, and Neisseria (N.) meningitidis using Real-Time PCR technology.Materials and methods: Two sets of primers were developed for detecting S. pneumoniae, H. influenzae, and N. meningitidis using the Primer Biosoft Allele ID 7.6 application. The study examined the minimum bacterial copy numbers detectable by each primer, as well as their specificity.Results: CtrA and hpd2 could detect the 400 copy numbers/ml of H. influenzae, and N. meningitidis and LytA2 could detect the 40 copy numbers/ml of S. pneumoniae. The sensitivity and specificity of all primers was 100% (CI: 95%).Conclusion: Using more sensitive primers to detect the bacterial agent responsible for causing bacterial meningitis increases the chance of identifying the causative bacteria. The primers designed in this study could identify the selected bacteria with at least 10 times more sensitivity than the currently available commercial diagnostic kits in Iran.","PeriodicalId":12738,"journal":{"name":"GMS Hygiene and Infection Control","volume":"20 ","pages":"Doc16"},"PeriodicalIF":1.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101131/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation, characterization, therapeutic and prophylactic applications of a lytic bacteriophage to combat multi-drug resistance Shigella flexneri: an animal study model. 一种抗多重耐药福氏志贺氏菌的裂解噬菌体的分离、鉴定、治疗和预防应用：动物研究模型

IF 1.7

GMS Hygiene and Infection Control Pub Date : 2025-04-30 eCollection Date: 2025-01-01 DOI: 10.3205/dgkh000543

Parisa Abbasi Fashami, Abazar Pournajaf, Nour Amirmozafari, Masoume Hallajzadeh, Vahid Pirhajati Mahabadi, Reza Saghiri, Soraya Khafri, Rezvan Golmoradi Zadeh, Sousan Akrami, Sajjad Asgharzadeh, Mehdi Rajabnia

{"title":"Isolation, characterization, therapeutic and prophylactic applications of a lytic bacteriophage to combat multi-drug resistance Shigella flexneri: an animal study model.","authors":"Parisa Abbasi Fashami, Abazar Pournajaf, Nour Amirmozafari, Masoume Hallajzadeh, Vahid Pirhajati Mahabadi, Reza Saghiri, Soraya Khafri, Rezvan Golmoradi Zadeh, Sousan Akrami, Sajjad Asgharzadeh, Mehdi Rajabnia","doi":"10.3205/dgkh000543","DOIUrl":"10.3205/dgkh000543","url":null,"abstract":"Background: Shigella (S.) flexneri is one of the most important causes of disease in children with diarrhea in Iran. Today, bacteriophages are an attractive option to resolve the drug resistance problem among pathogenic agents. Accordingly, the present study aimed to isolate a lytic bacteriophage of S. flexneri and investigate its impact on reducing excretion of Shigella in mouse models suffering from bacillary dysentery.Method: S. flexneri ATCC12022 was used. Identification of the phage isolated from hospital wastewater was performed using Transmission Electron Microscopy (TEM). Stability tests were performed to determine the sensitivity of isolated phages to various factors such as temperature, pH and bile salts. A male Syrian mouse model (C57), with mice 6 weeks of age weighing 22-25 g, was used to ensure safety and efficacy of the bacteriophage in reducing Shigella in stool. Treatment with the phage was performed (I) 1 h before, (II) 1 h after, (III) 5 h after, and (IV) 1 h before +1 h after bacterial challenge.Results: TEM indicated that the bacteriophage used in this study belongs to the Myoviridae family. Administration of one dose of bacteriophage before the infection can accelerate improvement post-transfection, and administration of bacteriophage post-infection has a therapeutic influence.Conclusion: In vivo and in vitro results indicate that our bacteriophage causes complete lysis of S. flexneri. Thus, this phage could be a therapeutic option for treating bacillary dysentery resulting from multidrug-resistant S. flexneri.","PeriodicalId":12738,"journal":{"name":"GMS Hygiene and Infection Control","volume":"20 ","pages":"Doc14"},"PeriodicalIF":1.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101133/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of antimicrobial efficacy of Acacia catechu mouthrinse and HiOra herbal mouthrinse and their influence on Streptococcus mutans count and Lactobacillus spp. count in children with early childhood caries. 儿茶口服液与HiOra草药口服液抑菌效果的比较及其对儿童早期龋齿变形链球菌和乳杆菌数量的影响。

IF 1.7

GMS Hygiene and Infection Control Pub Date : 2025-04-28 eCollection Date: 2025-01-01 DOI: 10.3205/dgkh000541

Madhura Joshi, Shruthi B Patil

{"title":"Comparison of antimicrobial efficacy of Acacia catechu mouthrinse and HiOra herbal mouthrinse and their influence on Streptococcus mutans count and Lactobacillus spp. count in children with early childhood caries.","authors":"Madhura Joshi, Shruthi B Patil","doi":"10.3205/dgkh000541","DOIUrl":"10.3205/dgkh000541","url":null,"abstract":"Background: Early childhood caries (ECC) is a chronic, infectious disease affecting young children. Though several preventive methods/measures are, the awareness about the benefits of a Ayurvedic preparation and its limited side effects is high. Thus, aim of this study was to compare efficacy of Ayurvedic formulations in reduction of the micro-organism causing ECC.Method: In a double-blind randomized, placebo-controlled trial 60 children between 6 and 71 months age with ECC were divided into group I (n=20, control group), group II (n=20, Acacia catechu group) and group III (n=20, HiOra group). Unstimulated saliva was collected pre and after 16th day post mouthrinse. The baseline microbiological colony count was performed for Streptococcus mutans and Lactobacillus spp. The results obtained were statistically analysed using non parametric test.Results: There was significant reduction in the amount of colony-forming units (cfu) between the control and the treatment groups. In the HiOra group the reduction of cfu was tendentially greater (p>0.05) than in the Acacia catechu group. In the HiOra group only significant reduction of Streptococcus (S.) mutans was seen, whereas in the Acacia catechu group significant reduction of S. mutans and Lactobacillus spp. was seen.Conclusion: Reduction in total microorganisms was less significant among the experimental groups. However the reduction in total colony count was greater in HiOra followed by Acacia catechu and the control group showed lesser reduction value.","PeriodicalId":12738,"journal":{"name":"GMS Hygiene and Infection Control","volume":"20 ","pages":"Doc12"},"PeriodicalIF":1.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101132/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The whole is greater than the sum of its parts: effect of implementing an ABS consultation service in the ABS program on antibiotic consumption at a tertiary military hospital. 整体大于部分之和：某三级军队医院在ABS项目中实施ABS咨询服务对抗生素消费的影响

IF 1.7

GMS Hygiene and Infection Control Pub Date : 2025-03-10 eCollection Date: 2025-01-01 DOI: 10.3205/dgkh000540

David Zeidler, Gwendolyn Scheumann, Claudia Baessler, Manuel Döhla, Dominic Preuß, Dominic Rauschning

{"title":"The whole is greater than the sum of its parts: effect of implementing an ABS consultation service in the ABS program on antibiotic consumption at a tertiary military hospital.","authors":"David Zeidler, Gwendolyn Scheumann, Claudia Baessler, Manuel Döhla, Dominic Preuß, Dominic Rauschning","doi":"10.3205/dgkh000540","DOIUrl":"https://doi.org/10.3205/dgkh000540","url":null,"abstract":"Background: Infections with multidrug resistant (MDR) pathogens represent an enormous challenge for the healthcare system. By implementing the principles of Antibiotic Stewardship (ABS), the rational use of antibiotics is promoted to counteract the increasing development of resistance on the one hand and to ensure adequate treatment for patients on the other.Methods: An interdisciplinary ABS team has therefore been set up at the Bundeswehr Central Hospital and is available to medical staff every working day as part of a consultation and ward-rounds service. The work of the ABS team primarily serves to advise on the treatment, diagnosis and prevention of infectious diseases. It also intends to provide knowledge during the training of young medical officers in order to promote the rational use of antibiotics even outside the Bundeswehr Central Hospital.Results: Since the implementation of the consultation and ward-rounds service, antibiotic consumption at the Bundeswehr Central Hospital has been reduced by 25%. In this context, the halving of carbapenem consumption is particularly noteworthy. Furthermore, the rising number of consultation requests shows an increasing need for the service and acts as a marker for ABS awareness.Conclusion: The reduction in antibiotic consumption is not solely due to the mentioned service, but is also the result of interdisciplinary ABS measures.","PeriodicalId":12738,"journal":{"name":"GMS Hygiene and Infection Control","volume":"20 ","pages":"Doc11"},"PeriodicalIF":1.7,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12060362/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143993173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing oropharyngeal swabbing techniques: the relationship between force applied and SARS-CoV-2 detection sensitivity. 优化口咽拭子技术：用力与SARS-CoV-2检测灵敏度的关系

IF 1.7

GMS Hygiene and Infection Control Pub Date : 2025-03-07 eCollection Date: 2025-01-01 DOI: 10.3205/dgkh000539

Peter Melcher, Corinna Pietsch, Sandra Bergs, Yasmin Youssef, Paul Rahden, Pierre Hepp, Ralf Henkelmann

{"title":"Optimizing oropharyngeal swabbing techniques: the relationship between force applied and SARS-CoV-2 detection sensitivity.","authors":"Peter Melcher, Corinna Pietsch, Sandra Bergs, Yasmin Youssef, Paul Rahden, Pierre Hepp, Ralf Henkelmann","doi":"10.3205/dgkh000539","DOIUrl":"https://doi.org/10.3205/dgkh000539","url":null,"abstract":"Aim: Nasopharyngeal and oropharyngeal swabs are essential for diagnosing SARS-CoV-2 infections, with nucleic acid testing (NAT) being the most sensitive method. However, NAT results are heavily influenced by preanalytical factors, including quality of the sample. This study examines the effect of applied force during oropharyngeal sampling on sample quality, specifically assessing cell count and the associated NAT cycle threshold (Ct) values.Methods: A three-phase investigation was conducted to explore the relationship between sampling force and cell quantity, as well as the impact of cell count on NAT sensitivity.Results: A significantly lower Ct value was achieved by artificially increasing the cell count in a swab sample and applying a greater force resulted in higher cell counts, but the opposite effect on Ct values of SARS-CoV-2 NAT was shown.Conclusion: These findings indicate that while applying greater force during sample collection increases the number of collected cells, it does not improve the sensitivity of SARS-CoV-2 detection and can even lead to poorer results. Further research should focus on optimizing swab design to improve sample quality and the number of cells obtained.","PeriodicalId":12738,"journal":{"name":"GMS Hygiene and Infection Control","volume":"20 ","pages":"Doc10"},"PeriodicalIF":1.7,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12060361/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143963199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral mucosal lesions in pediatric patients. 儿科患者口腔黏膜病变。

IF 1.7

GMS Hygiene and Infection Control Pub Date : 2025-03-06 eCollection Date: 2025-01-01 DOI: 10.3205/dgkh000536

Vasudevi Ramiah, Devaki Manivannan, Karthik Shunmugavelu, Barun Kumar, Evangeline Cynthia Dhinakaran

引用次数: 0

Molecular detection of phenol-soluble modulin-mec (PSM-mec) in Staphylococcus aureus clinical isolates from Federal Medical Center Birnin Kebbi, North-West, Nigeria. 尼日利亚西北部Birnin Kebbi联邦医学中心金黄色葡萄球菌临床分离株酚溶性调素-mec的分子检测

IF 1.7

GMS Hygiene and Infection Control Pub Date : 2025-03-06 eCollection Date: 2025-01-01 DOI: 10.3205/dgkh000538

Isah Musa Maishanu, Adeshina O Gbonjubola, Hussaini Mujahid, Busayo O Olayinka

{"title":"Molecular detection of phenol-soluble modulin-mec (PSM-mec) in Staphylococcus aureus clinical isolates from Federal Medical Center Birnin Kebbi, North-West, Nigeria.","authors":"Isah Musa Maishanu, Adeshina O Gbonjubola, Hussaini Mujahid, Busayo O Olayinka","doi":"10.3205/dgkh000538","DOIUrl":"https://doi.org/10.3205/dgkh000538","url":null,"abstract":"Aim: This study was carried out to isolate and detect virulence genes associated with Staphylococcus (S.) aureus clinical isolates from the Federal Medical Center Birnin Kebbi, Nigeria.Methods: To obtain S. aureus isolates, samples were taken from urine, sputum, blood and wound sources. S. aureus was phenotypically identified using Microgen staph ID system and PSM-mec and PVL genes were detected using polymerase chain reaction (PCR).Results: A total of 48 non-duplicate S. aureus isolates were obtained (21 from wound swabs, 7 from blood, 15 from urine, and 5 from sputum). From the 14 S. aureus isolates examined by PCR, the most abundant gene was PSM-mec (42.8%), while the PVL was the least abundant with 21.4%.Conclusion: Because it gives highly specific and accurate results, it is essential to use the PCR technique to detect S. aureus virulence determinants as well as PSM-mec and PVL as targets for antimicrobial agents.","PeriodicalId":12738,"journal":{"name":"GMS Hygiene and Infection Control","volume":"20 ","pages":"Doc09"},"PeriodicalIF":1.7,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12059804/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144013626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Letter to the editor regarding the paper "S2k-Guideline hand antisepsis and hand hygiene". 就《s2k手部消毒和手部卫生指南》一文致编辑的信。

IF 1.7

GMS Hygiene and Infection Control Pub Date : 2025-03-06 eCollection Date: 2025-01-01 DOI: 10.3205/dgkh000537

Maren Eggers, Katrin Steinhauer, Florin H H Brill

引用次数: 0