Genetika最新文献_第7页

[Analysis of complete sequence of cryptic plasmid pTP33 from Yersinia pestis isolated in Tuva natural focus of plague]. [图瓦鼠疫自然疫源地鼠疫耶尔森菌隐质粒pTP33全序列分析]。

Genetika Pub Date : 2016-09-01

M V Afanas’ev, S V Balakhonov, E G Tokmakova, V S Polovinkina, E A Sidorova, V V Sinkov

{"title":"[Analysis of complete sequence of cryptic plasmid pTP33 from Yersinia pestis isolated in Tuva natural focus of plague].","authors":"M V Afanas’ev, S V Balakhonov, E G Tokmakova, V S Polovinkina, E A Sidorova, V V Sinkov","doi":"","DOIUrl":"","url":null,"abstract":"This paper studies a full nucleotide sequence of cryptic plasmid pTP33, which was isolated from the typical plague strain of the Tuvinian natural focus, Yersinia pestis I-2638. Sequencing was carried out using the 454 GS Junior platform (Roche). In analysis using the software package GS De Novo Assembler v. 2.7 (Roche) and the algorithm Newbler v. 2.7, 1855 nucleotide reads, which contained 1101246 nucleotides, were assembled to a contig of 33 978 bp. The GC content of the obtained nucleotide sequence was 50.25%. During annotation, we found 56 open reading frames. Homologs of the predicted reading frames were sought in the BLAST databases. We detected 22 reading frames coding hypothetical proteins, 23 frames coding phagerelated proteins, and 11 frames coding proteins with known functions, including toxin–antitoxin system YefM-YoeB, nucleic acids and polysaccharides metabolism proteins (exopolysaccharide production protein ExoZ, exodeoxyribonuclease VIII), and replication proteins (ParA). Some predicted pTP33 proteins were found to be homologs (from 45 to 75%) with sequences of phage-related proteins of certain microorganisms—endosymbionts of insects (Sodalis glossinidius) and endosymbionts of entomopathogenic nematodes (Photorhabdus luminescens, P. asymbiotica, Xenorhabdus bovienii).","PeriodicalId":12707,"journal":{"name":"Genetika","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35767471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Molecular phylogenetic analysis of the ant genus Formica L. (Hymenoptera: Formicidae) from Palearctic region]. [古北地区蚁属蚁群的分子系统发育分析[j]。

Genetika Pub Date : 2016-08-01

I A Antonov, Yu S Bukin

引用次数: 0

[Molecular genetic analysis of five extant reserves of black honeybee Apis melifera melifera in the Urals and the Volga region]. 乌拉尔和伏尔加河地区现存5个黑蜜蜂保护区的分子遗传分析。

Genetika Pub Date : 2016-08-01

R A Ilyasov, A V Poskryakov, A V Petukhov, A G Nikolenko

{"title":"[Molecular genetic analysis of five extant reserves of black honeybee Apis melifera melifera in the Urals and the Volga region].","authors":"R A Ilyasov, A V Poskryakov, A V Petukhov, A G Nikolenko","doi":"","DOIUrl":"","url":null,"abstract":"Local populations of the black honeybee Apis mellifera mellifera from the Urals and the Volga region were examined in comparison with local populations of southern honeybee subspecies A. m. caucasica and A. m. carpatica from the Caucasus and the Carpathians. Genetic analysis was performed on the basis of the polymorphism of nine microsatellite loci of nuclear DNA and the mtDNA COI–COII locus. On the territory of the Urals and the Volga region, five extant populations (reserves) of the black honeybee A. m. mellifera were identified, including the Burzyanskaya, Tatyshlinskaya, Yuzhno-Prikamskaya, Visherskaya, and Kambarskaya populations. These five populations are the basis of the modern gene pool of the black honeybee A. m. mellifera from the Urals and the Volga region. The greatest proportion of the remaining indigenous gene pool of A. m. mellifera (the core of the gene pool of the population of A. m. mellifera) is distributed over the entire territory of Perm krai and the north of the Republic of Bashkortostan. For the population of A. m. mellifera from the Urals and the Volga region, the genetic standards were calculated, which will be useful for future population studies of honeybees.","PeriodicalId":12707,"journal":{"name":"Genetika","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35766881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Inheritance of longitudinal white shell bands in the snail Littorina obtusata (Gastropoda, Prosobranchia)]. [腹足纲，前鳃纲)蜗牛白壳纵向带的遗传]。

Genetika Pub Date : 2016-08-01

E V Kozminsky

引用次数: 0

[The heteroplasmy level of some mutations in gene MT-CYB among women with asymptomatic atherosclerosis]. 【MT-CYB基因突变在无症状动脉粥样硬化女性中的异质性水平】。

Genetika Pub Date : 2016-08-01

V V Sinyov, M M Chicheva, V A Barinova, A I Ryzhkova, R I Zilinyi, V P Karagodin, A Yu Postnov, I A Sobenin, A N Orekhov, M A Sazonova

{"title":"[The heteroplasmy level of some mutations in gene MT-CYB among women with asymptomatic atherosclerosis].","authors":"V V Sinyov, M M Chicheva, V A Barinova, A I Ryzhkova, R I Zilinyi, V P Karagodin, A Yu Postnov, I A Sobenin, A N Orekhov, M A Sazonova","doi":"","DOIUrl":"","url":null,"abstract":"Atherosclerosis is a polygenic socially significant disease whose risk factors include coronary heart disease, diabetes, hypertension, and myocardial infarction. According to the literature, mutations m.14846G>A (G34S), m.15762G>A (G339Q), m.15084G>A (W113Ter), and m.15059G>A (G190Ter) of cytochrome B gene (MT-CYB) are associated with mitochondrial myopathies, myoglobinuria, and exercise intolerance. Preliminary studies carried out by the authors made it possible to discover an association of certain mitochondrial genome mutations with atherosclerotic lesions of aortic intima in people who died as a result of an accident or sudden death. The most interesting seemed to be the data on the association of mutations m.14846G>A and m.15059G>A of the cytochrome B gene with lipofibrous aortic plaques, because these mutations affect the mitochondrial respiratory chain enzyme. Defects in the given chain may be the reason for the launch of pathogenic mechanisms in the human body. Owing to the fact that mutations in the mitochondrial genome are inherited by the maternal type, it was decided to analyze cytochrome B gene mutations in a sample of female volunteers from Moscow oblast. According to the findings, mutations m.14846G>A and m.15059G>A are highly significantly associated with atherosclerotic lesions of the carotid arteries: m.14846G>A is antiatherogenic and m.15059G>A is proatherogenic.","PeriodicalId":12707,"journal":{"name":"Genetika","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35765779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Phylogenetic relationships of the species of Oxytropis DC. subg. Oxytropis and Phacoxytropis (Fabaceae) from Asian Russia inferred from the nucleotide sequence analysis of the intergenic spacers of the chloroplast genome]. 棘豆属植物的系统发育关系。subg。根据叶绿体基因组基因间间隔序列的核苷酸序列分析，推断产于俄罗斯亚洲的棘豆属(Oxytropis)和棘豆属(phaacoxytropis)。

Genetika Pub Date : 2016-08-01

A B Kholina, M M Kozyrenko, E V Artyukova, D V Sandanov, E A Andrianova

{"title":"[Phylogenetic relationships of the species of Oxytropis DC. subg. Oxytropis and Phacoxytropis (Fabaceae) from Asian Russia inferred from the nucleotide sequence analysis of the intergenic spacers of the chloroplast genome].","authors":"A B Kholina, M M Kozyrenko, E V Artyukova, D V Sandanov, E A Andrianova","doi":"","DOIUrl":"","url":null,"abstract":"The nucleotide sequence analysis of trnH–psbA, trnL–trnF, and trnS–trnG intergenic spacer regions of chloroplast DNA performed in the representatives of the genus Oxytropis from Asian Russia provided clarification of the phylogenetic relationships of some species and sections in the subgenera Oxytropis and Phacoxytropis and in the genus Oxytropis as a whole. Only the section Mesogaea corresponds to the subgenus Phacoxytropis, while the section Janthina of the same subgenus groups together with the sections of the subgenus Oxytropis. The sections Chrysantha and Ortholoma of the subgenus Oxytropis are not only closely related to each other, but together with the section Mesogaea, they are grouped into the subgenus Phacoxytropis. It seems likely that the sections Chrysantha and Ortholoma should be assigned to the subgenus Phacoxytropis, and the section Janthina should be assigned to the subgenus Oxytropis. The molecular differences were identified between O. coerulea and O. mandshurica from the section Janthina that were indicative of considerable divergence of their chloroplast genomes and the species independence of the taxa. The species independence of O. czukotica belonging to the section Arctobia was also confirmed.","PeriodicalId":12707,"journal":{"name":"Genetika","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35765992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Genetic structure of the Russian populations of Pyrenophora tritici-repentis, determined by using microsatellite markers]. [利用微卫星标记确定的俄罗斯白蛉种群遗传结构]。

Genetika Pub Date : 2016-08-01

N V Mironenko, O A Baranova, N M Kovalenko, L A Mikhailova, L P Rosseva

{"title":"[Genetic structure of the Russian populations of Pyrenophora tritici-repentis, determined by using microsatellite markers].","authors":"N V Mironenko, O A Baranova, N M Kovalenko, L A Mikhailova, L P Rosseva","doi":"","DOIUrl":"","url":null,"abstract":"The population genetic structure of plant pathogenic fungus Pyrenophora tritici-repentis was examined using microsatellite (SSR) markers. According to the geographical origin of the pathogen populations, they were designated as North Caucasian (S, 33 isolates), northwest (Nw, 39), and Omsk (Om, 43). The populations were analyzed at the nine most polymorphic SSR loci, at which 75 alleles were identified. To characterize the genetic variation within and between populations, the AMOVA algorithm as implemented in the Arlequin v. 3.5 software program was used. The number of alleles per locus ranged from 5 to 12 and their sizes varied within the range from 180 to 400 bp. The mean gene diversity at SSR loci was high for all populations (H = 0.58–0.75). The populations were considerably different in the frequencies of individual alleles of the SSR loci. Most isolates in the populations were represented by unique haplotypes. The within-population variation of the isolates at molecular markers was 86.4%; among the populations, 13.6%. Substantial interpopulation differences were found between the Om and S (F st = 0.16) and between the Om and Nw (F st = 0.20) populations, whereas between the S and Nw populations, these differences were small (F st = 0.05). Thus, it was demonstrated that the population of P. tritici-repentis from Omsk oblast had the independent status of the geographical population; northwest and North Caucasian populations differed in the allelic diversity of SSR loci, and despite the low F st value (0.05), they also belonged to independent geographical populations.","PeriodicalId":12707,"journal":{"name":"Genetika","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35766079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Association of polymorphic markers of chemokine genes, their receptors, and CD14 gene with coronary atherosclerosis]. [趋化因子基因多态性标记物及其受体和CD14基因与冠状动脉粥样硬化的关系]。

Genetika Pub Date : 2016-08-01

T R Nasibullin, L F Yagafarova, I R Yagafarov, Ya R Timasheva, V V Erdman, I A Tuktarova, O E Mustafina

{"title":"[Association of polymorphic markers of chemokine genes, their receptors, and CD14 gene with coronary atherosclerosis].","authors":"T R Nasibullin, L F Yagafarova, I R Yagafarov, Ya R Timasheva, V V Erdman, I A Tuktarova, O E Mustafina","doi":"","DOIUrl":"","url":null,"abstract":"Atherosclerosis represents an inflammatory response to the disturbance of the endothelial layer in the arterial bloodstream. In the present study, an analysis of associations of polymorphic markers for the genes controlling synthesis of proteins involved in atherosclerosis pathogenesis in coronary atherosclerosis (CA) patients (217 subjects) and in a control group (250 subjects) was conducted. The following genes were examined: rs991804 (CCL2 gene), rs1126579 (CXCR2 gene), rs4074 (CXCL1 gene), rs4073 (CXCL8 gene), rs333 (CCR5 gene), rs2471859 (CXCR4 gene), rs1801157 (CXCL12 gene), and rs2569190 (CD14 gene). Using the Monte Carlo and Markov chain (APSampler) method, allele/genotype combinations associated with both low and high CA risk were revealed. The most important findings included the following: CXCR4*T/T + CCL2*C + CCR5*I/I (P perm = 1 × 10–6, OR = 0.44, 95% CI 0.3–0.63), CXCR2*C + CD14*C + CXCL12*G + CCL2*C + CCR5*D (P perm = 4 × 10–6, OR = 5.78, 95% CI 2.34–14.28), CD14*C + CCL2*C/C + CCR5*D (P perm = 6.3 × 10–6, OR = 5.81, 95% CI 2.17–15.56), CXCL8*A + CXCR2*C + CD14*T + CXCR4*C (P perm = 0.01, OR = 3.21, 95% CI 1.63–6.31).","PeriodicalId":12707,"journal":{"name":"Genetika","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35767464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Manifestation of multimodality in a simple ecological-genetic model of population evolution]. [多模态在种群进化的简单生态遗传模型中的表现]。

Genetika Pub Date : 2016-08-01

O L Zhdanova, E Ya Frisman

引用次数: 0

[Association of variable rs1801282 locus of PPARG2 gene with diabetic nephropathy]. 【PPARG2基因rs1801282位点变异与糖尿病肾病的关系】。

Genetika Pub Date : 2016-08-01

D Sh Avzaletdinova, L F Sharipova, O V Kochetova, T V Morugova, V V Erdman, O E Mustafina

引用次数: 0