Genetika最新文献_第10页

[Sequencing and analysis of the resistome of Streptomyces fradiae ATCC19609 in order to develop a test system for screening of new antimicrobial agents]. [对传统链霉菌ATCC19609的抗性组进行测序和分析，以开发新的抗菌药物筛选测试系统]。

Genetika Pub Date : 2016-06-01

A A Vatlin, O B Bekker, L N Lysenkova, A M Korolev, A E Shchekotikhin, V N Danilenko

{"title":"[Sequencing and analysis of the resistome of Streptomyces fradiae ATCC19609 in order to develop a test system for screening of new antimicrobial agents].","authors":"A A Vatlin, O B Bekker, L N Lysenkova, A M Korolev, A E Shchekotikhin, V N Danilenko","doi":"","DOIUrl":"","url":null,"abstract":"The paper provides the annotation and data on sequencing the antibiotic resistance genes in Streptomyces fradiae strain ATCC19609, highly sensitive to different antibiotics. Genome analysis revealed four groups of genes that determined the resistome of the tested strain. These included classical antibiotic resistance genes (nine aminoglycoside phosphotransferase genes, two beta-lactamase genes, and the genes of puromycin N-acetyltransferase, phosphinothricin N-acetyltransferase, and aminoglycoside acetyltransferase); the genes of ATP-dependent ABC transporters, involved in the efflux of antibiotics from the cell (MacB-2, BcrA, two-subunit MDR1); the genes of positive and negative regulation of transcription (whiB and padR families); and the genes of post-translational modification (serine-threonine protein kinases). A comparative characteristic of aminoglycoside phosphotransferase genes in S. fradiae ATCC19609, S. lividans TK24, and S. albus J1074, the causative agent of actinomycosis, is provided. The possibility of using the S. fradiae strain ATCC19609 as the test system for selection of the macrolide antibiotic oligomycin A derivatives with different levels of activity is demonstrated. Analysis of more than 20 semisynthetic oligomycin A derivatives made it possible to divide them into three groups according to the level of activity: inactive (>1 nmol/disk), 10 substances; with medium activity level (0.05–1 nmol/disk), 12 substances; and more active (0.01–0.05 nmol/disk), 2 substances. Important for the activity of semisynthetic derivatives is the change in the position of the 33rd carbon atom in the oligomycin A molecule.","PeriodicalId":12707,"journal":{"name":"Genetika","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35765764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Two novel mutations in gene SPG4 in patients with autosomal dominant spastic paraplegia]. [常染色体显性痉挛性截瘫患者SPG4基因的两种新突变]。

Genetika Pub Date : 2016-06-01

A F Akhmetgaleyeva, I M Khidiyatova, E V Saifullina, R F Idrisova, R V Magzhanov, E K Khusnutdinova

引用次数: 0

[Aggression and empathy as genetic differentiation factors of urban population]. [攻击性和共情作为城市人群的遗传分化因素]。

Genetika Pub Date : 2016-06-01

L A Atramentova, E N Luchko

引用次数: 0

[Current state of the genetic polymorphism in spring barley (Hordeum vulgare L.) from Russia assessed by the alleles of hordein-coding loci]. [利用大麦蛋白编码位点的等位基因评估俄罗斯春大麦（Hordeum vulgare L.）遗传多态性的现状]。

Genetika Pub Date : 2016-06-01

E V Lyalina, S V . Boldyrev, A A Pomortsev

{"title":"[Current state of the genetic polymorphism in spring barley (Hordeum vulgare L.) from Russia assessed by the alleles of hordein-coding loci].","authors":"E V Lyalina, S V . Boldyrev, A A Pomortsev","doi":"","DOIUrl":"","url":null,"abstract":"Starch gel electrophoresis was performed to study the polymorphism of hordeins encoded by the Hrd A, Hrd B, and Hrd F loci in 211 varieties of spring barley. For 41 of these varieties, the genetic formulas were established for the first time. In the two samples of varieties, the comparative analysis of allelic diversity and allele frequencies of hordein-coding loci was carried out. The first sample consisted of 101 spring barley varieties approved for the use on the territory of the Russian Federation in 1999, while the second sample included 160 spring barley varieties that were approved in 2014; 49 of these varieties were common for both samples. It is demonstrated that the current tendency to reduction of the proportion of heterogeneous spring barley varieties is mainly due to the introduction of foreign varieties homogeneous for the hordein-coding loci. At the same time, there is an increase in polymorphism of hordein-coding loci in modern spring barley varieties. The number of alleles for the Hrd A locus increased by five alleles, and for the Hrd B locus, by nine alleles. Along with the alleles recorded earlier in barley landrace populations and varieties bred in 20th century, three novel alleles of the Hrd A locus and four alleles of the Hrd B locus were identified. The number of alleles of the Hrd F locus remained unchanged (four), and the changes in their frequencies were small. At the same time, the changes in frequency observed for some alleles of the Hrd A and Hrd B loci were statistically significant. All newly identified alleles of hordein-coding loci were found with low frequencies (from 0.003 to 0.006), so despite the increased number of alleles, no statistically significant increase in genetic diversity in terms of μ and PIC indices was observed.","PeriodicalId":12707,"journal":{"name":"Genetika","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35766944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Polymorphism of CD209 and TLR3 genes in populations of North Eurasia]. [CD209和TLR3基因在欧亚大陆北部人群中的多态性]。

Genetika Pub Date : 2016-06-01

A V Barkhash, V N Babenko, M I Voevoda, A G Romaschenko

{"title":"[Polymorphism of CD209 and TLR3 genes in populations of North Eurasia].","authors":"A V Barkhash, V N Babenko, M I Voevoda, A G Romaschenko","doi":"","DOIUrl":"","url":null,"abstract":"The DC-SIGN (dendritic cell-specific intercellular adhesion molecule (ICAM)-3-grabbing non-integrin) and TLR3 (toll-like receptor 3) proteins are key effectors of the innate immunity and particularly play an important role in the organism’s antiviral defense as pattern-recognition receptors. Previously, we demonstrated that certain genotypes and alleles of single nucleotide polymorphisms (SNPs) rs2287886 (G/A) in the promoter region of the CD209 gene (encoding DC-SIGN) and rs3775291 (G/A, Leu412Phe) in the exon 4 of the TLR3 gene are associated with human predisposition to tick-borne encephalitis in the Russian population. In the present work, the distribution of genotype and allele frequencies for these SNPs was studied in seven populations of North Eurasia, including Caucasians (Russians and Germans (from Altai region)), Central Asian Mongoloids (Altaians, Khakass, Tuvinians, and Shorians), and Arctic Mongoloids (Chukchi). It was found that the CD209 gene rs2287886 SNP A/A genotype and A allele, as well as the TLR3 gene rs3775291 SNP G/G genotype and G allele (the frequencies of which in our previous studies were increased in tick-borne encephalitis patients as compared with the population control (Russian citizens of Novosibirsk)), are preserved with a high frequency in Central Asian Mongoloids (who for a long time regularly came in contact with tick-borne encephalitis virus in places of their habitation). We suggested that predisposition to tick-borne encephalitis in Central Asian Mongoloid populations can be predetermined by a different set of genes and their polymorphisms than in the Russian population.","PeriodicalId":12707,"journal":{"name":"Genetika","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35766871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Genetic polymorphism, haplotype distribution, and phylogeny of Daphnia (Cladocera: Anomopoda) species from the water bodies of russia as inferred from the 16S mtDNA gene sequencing]. [从16S mtDNA基因测序推断的俄罗斯水体水蚤(枝总目:无足目)物种的遗传多态性、单倍型分布和系统发育]。

Genetika Pub Date : 2016-06-01

E I Zuykova, N A Bochkarev, N G Sheveleva

引用次数: 0

[The haplomatch program for comparing Y-chromosome STR-haplotypes and its application to the analysis of the origin of Don Cossacks]. [用于比较y染色体str -单倍型的单倍配型程序及其在顿河哥萨克血统分析中的应用]。

Genetika Pub Date : 2016-05-01

M I Chukhryaeva, I O Ivanov, S A Frolova, S M Koshel, O M Utevska, R A Skhalyakho, A T Agdzhoyan, Yu V Bogunov, E V Balanovska, O P Balanovsky

{"title":"[The haplomatch program for comparing Y-chromosome STR-haplotypes and its application to the analysis of the origin of Don Cossacks].","authors":"M I Chukhryaeva, I O Ivanov, S A Frolova, S M Koshel, O M Utevska, R A Skhalyakho, A T Agdzhoyan, Yu V Bogunov, E V Balanovska, O P Balanovsky","doi":"","DOIUrl":"","url":null,"abstract":"STR haplotypes of the Y chromosome are widely used as effective genetic markers in studies of human populations and in forensic DNA analysis. The task often arises to compare the spectrum of haplotypes in individuals or entire populations. Performing this task manually is too laborious and thus unrealistic. We propose an algorithm for counting similarity between STR haplotypes. This algorithm is suitable for massive analyses of samples. It is implemented in the computer program Haplomatch, which makes it possible to find haplotypes that differ from the target haplotype by 0, 1, 2, 3, or more mutational steps. The program may operate in two modes: comparison of individuals and comparison of populations. Flexibility of the program (the possibility of using any external database), its usability (MS Excel spreadsheets are used), and the capability of being applied to other chromosomes and other species could make this software a new useful tool in population genetics and forensic and genealogical studies. The Haplomatch software is freely available on our website www.genofond.ru. The program is applied to studying the gene pool of Cossacks. Experimental analysis of Y-chromosomal diversity in a representative set (N = 131) of Upper Don Cossacks is performed. Analysis of the STR haplotypes detects genetic proximity of Cossacks to East Slavic populations (in particular, to Southern and Central Russians, as well as to Ukrainians), which confirms the hypothesis of the origin of the Cossacks mainly due to immigration from Russia and Ukraine. Also, a small genetic influence of Turkicspeaking Nogais is found, probably caused by their occurrence in the Don Voisko as part of the Tatar layer. No similarities between haplotype spectra of Cossacks and Caucasus populations are found. This case study demonstrates the effectiveness of the Haplomatch software in analyzing large sets of STR haplotypes.","PeriodicalId":12707,"journal":{"name":"Genetika","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35765506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Catechol-oxide-methyltransferase (COMT rs4680:G>A) gene polymorphism does not affect analgesics’ demand after elective hip replaceme. 儿茶酚-氧化物-甲基转移酶(COMT rs4680:G>A)基因多态性不影响选择性髋关节置换术后镇痛药的需求。

Genetika Pub Date : 2016-05-01 DOI: 10.7868/s0016675816030048

M Białecka, A Jurewicz, P Cięszczyk, A Machoy-Mokrzyńska, M Kurzawski, K Leźnicka, V Dziedziejko, K Safranow, M Droździk, A Bohatyrewicz

{"title":"Catechol-oxide-methyltransferase (COMT rs4680:G>A) gene polymorphism does not affect analgesics’ demand after elective hip replaceme.","authors":"M Białecka, A Jurewicz, P Cięszczyk, A Machoy-Mokrzyńska, M Kurzawski, K Leźnicka, V Dziedziejko, K Safranow, M Droździk, A Bohatyrewicz","doi":"10.7868/s0016675816030048","DOIUrl":"https://doi.org/10.7868/s0016675816030048","url":null,"abstract":"Pain in patients with hip osteoarthritis appears long before surgery, and requires effective management as it affects patient comfort and daily activities. Therefore, the search for factors influencing response rate to analgesics is mandatory. In recent years, increasing attention has been paid to genetic factors underlying pain threshold and treatment efficacy. Polymorphic gene of catechol-oxide-methyltransferase (COMT) is a candidate gene associated with pain pathology and treatment response. The aim of the study was to evaluate association between the COMT rs4680:G>A polymorphism and demand for analgesics in patients subjected to elective hip replacement. The study included 196 patients after hip replacement surgery. Opioid demand was recorded and analgesic efficacy was scored using a four-level verbal pain intensity scale. COMT rs4680:G>A polymorphism was analysed by PCR-RFLP method. The studied COMT genotypes did not influence opioid administration in the studied patients from the day of surgery till day 6 afterwards. The distribution of the COMT rs4680:G>A in the studied subjects was as follows: GA—52.04%, AA—23.98% and GG—23.98%. It can be concluded that the COMT rs4680:G>A polymorphism is not associated with opioid demand in patients after elective hip replacement.","PeriodicalId":12707,"journal":{"name":"Genetika","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35765956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Species divergence in Iris series Lacteae (Iridaceae) in Russia and adjacent countries based on chloroplast DNA sequence data]. [基于叶绿体DNA序列数据的俄罗斯及邻近国家鸢尾科(鸢尾科)物种分化]。

Genetika Pub Date : 2016-05-01

E V Boltenkov, E V Artyukova, M M Kozyrenko

引用次数: 0

[The role of ATP-dependent chromatin remodeling complexes in regulation of genetic processes]. [atp依赖性染色质重塑复合物在调控遗传过程中的作用]。

Genetika Pub Date : 2016-05-01

M Yu Mazina, N E Vorobyeva

引用次数: 0