Gene最新文献

{"title":"Optimizing the delivery of CRISPR/Cas9 ribonucleoproteins for efficient genome editing in bovine embryos.","authors":"Xiaofeng Du, Alexander Quinn, Moira Menzies, Laercio R Porto-Neto","doi":"10.1016/j.gene.2025.149715","DOIUrl":"10.1016/j.gene.2025.149715","url":null,"abstract":"<p><p>To assist in establishing a streamlined and efficient workflow for generating gene-edited bovine embryos, we evaluated three transfection approaches for the delivery of CRISPR Cas9-sgRNA ribonucleoproteins into bovine zygotes: lipofection with Lipofectamine CRISPRMAX, and electroporation using either Neon or NEPA21 electroporation systems. Bos taurus prolactin receptor (PRLR) was used as the target gene. The PRLR editing outcomes were analysed by PCR genotyping and Sanger sequencing of individual embryos at day 8 post-fertilization. CRISPRMAX transfection generated up to 30 % PRLR-edited blastocysts (8 % homozygous deletion), without affecting the embryo cleavage (93 %) and blastocyst rate (39 %) relative to non-transfected controls. For both NEPA21 and Neon electroporation, we found that increasing the voltage, length and number of pulses resulted in enhanced gene editing efficiency but compromised embryo cleavage and blastocyst rates. NEPA21 electroporation with a commercial electroporation enhancer reagent produced up to 47.6 % transfected embryos with the PRLR deletion, but with decreased embryo cleavage (62 %) and blastocyst (18 %) rates. Combining NEPA21 electroporation with CRISPRMAX lipofection enhanced the gene editing efficiency to 50 % (23 % homozygous editing), with 64 % embryo cleavage rate and 18 % blastocyst rate. Notably, Neon electroporation resulted in 65.2 % PRLR-edited blastocysts with 21 % homozygous editing (50% cleavage rate and 10 % blastocyst rate). Additional studies may be necessary to further optimize electroporation parameters to achieve an optimal balance between embryo viability and gene editing efficiency. These outcomes will provide valuable insights for improving gene editing workflows for bovines and may help to promote and accelerate the widespread implementation of genome editing technology in livestock.</p>","PeriodicalId":12499,"journal":{"name":"Gene","volume":" ","pages":"149715"},"PeriodicalIF":2.4,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The important role of stress granules in prostate cancer development, progression, and drug resistance","authors":"Zhendong Xu ,&nbsp;Hongjie You ,&nbsp;Jun Gu ,&nbsp;Junhui Jiang ,&nbsp;Zejun Yan ,&nbsp;Xiaofeng Jin","doi":"10.1016/j.gene.2025.149776","DOIUrl":"10.1016/j.gene.2025.149776","url":null,"abstract":"<div><div>Prostate cancer (PCa) is the second most prevalent malignancy (7.3 %) and fifth leading cause of cancer death (4.1 %) in men globally. While lung cancer remains the predominant cancer in both incidence and mortality among all cancers, PCa exhibits geographically heterogeneous rising trends. Stress granules (SGs) are membraneless organelles formed through liquid–liquid phase separation (LLPS), playing a pivotal role in cellular stress responses, and are closely associated with various cancers, including PCa. Studies have shown that the expression of key SG-nucleating proteins, such as Ras-GTPase-activating protein-binding protein 1 (G3BP1), is upregulated in PCa, promoting the assembly of SGs. SGs can facilitate the initiation and progression of PCa by regulating mRNA stability, gene expression, and cellular signaling pathways, while also protecting cancer cells from damage under various stress conditions. Furthermore, SGs can modulate androgen receptor (AR) signaling, influencing PCa cell survival and sensitivity to androgen deprivation therapy (ADT). Additionally, SGs can promote PCa resistance to chemotherapy, including docetaxel (DTX), through interactions with various molecules involved in apoptosis, autophagy, and metabolism. This review summarizes the roles of SGs in the development, progression, and drug resistance of PCa, building on current advances in targeting SGs, highlights their promising potential as novel therapeutic targets for inhibiting malignant cancer progression, overcoming therapeutic resistance, and advancing PCa treatment strategies.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"970 ","pages":"Article 149776"},"PeriodicalIF":2.4,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145091649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “The Salvia miltiorrhiza NAC transcription factor SmNAC1 enhances zinc content in transgenic Arabidopsis”. [Gene 688 (2019) 54–61]","authors":"Bin Zhu ,&nbsp;Dong-Ao Huo ,&nbsp;Xiao-Xiao Hong ,&nbsp;Juan Guo ,&nbsp;Tao Peng ,&nbsp;Jie Liu ,&nbsp;Xiao-Long Huang ,&nbsp;Hui-Qing Yan ,&nbsp;Qing-Bei Weng ,&nbsp;Xiao-Cun Zhang ,&nbsp;Xu-Ye Du","doi":"10.1016/j.gene.2025.149757","DOIUrl":"10.1016/j.gene.2025.149757","url":null,"abstract":"","PeriodicalId":12499,"journal":{"name":"Gene","volume":"968 ","pages":"Article 149757"},"PeriodicalIF":2.4,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145080453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The anti-aging mechanisms of Catalpol by elevating mitophagy","authors":"Songtao Bie ,&nbsp;Ze Yao ,&nbsp;Ming Lu ,&nbsp;Chunshuang Li ,&nbsp;Xiang Li ,&nbsp;Hui Shang","doi":"10.1016/j.gene.2025.149765","DOIUrl":"10.1016/j.gene.2025.149765","url":null,"abstract":"<div><div>Autophagy is an important mechanism for maintaining cell homeostasis, and its decline in function is widely regarded as a key factor in aging. Catalpol (CAT), an active compound derived from the traditional Chinese herb<!--> <em>Rehmannia glutinosa</em>, has shown potential to treat various age-related diseases through the regulation of autophagy. However, how CAT exerts anti-aging effects by regulating autophagy remains unclear. Therefore, this study sought to explore the anti-aging potential and molecular mechanisms, particularly the role of CAT in autophagy and mitochondrial function in yeast cells. The PI-CLS measurement method was employed to demonstrate enhanced yeast cells viability upon CAT treatment. RNA-Seq combined with bioinformatics analyses identified <em>HSP82</em> as a key target of CAT. <em>HSP82</em> knockout and <em>HSP82</em> overexpression strains were employed to demonstrate that CAT enhances mitochondrial functions and extends cells lifespan by inducing <em>HSP82</em>-dependent mitophagy. We concluded that CAT plays an anti-aging role by regulating <em>HSP82</em>-dependent mitophagy and mitochondrial functions in yeast cells.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"969 ","pages":"Article 149765"},"PeriodicalIF":2.4,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145085759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aging-associated transcriptomic changes in the bovine endometrium","authors":"Denis Karani Wanjiru ,&nbsp;Yvan Bienvenu Niyonzima ,&nbsp;Yoichi Mizukami ,&nbsp;Hiroya Kadokawa","doi":"10.1016/j.gene.2025.149764","DOIUrl":"10.1016/j.gene.2025.149764","url":null,"abstract":"<div><div>Proper endometrial function is essential for fertility, but aging impairs endometrial activity through mechanisms that remain unclear in cattle. This study aimed to identify age-related differentially expressed genes (DEGs) in the endometrium of approximately 121-month-old multiparous Japanese Black cows (N = 5) compared with approximately 24-month-old nulliparous heifers (N = 5), using RNA sequencing. A total of 20,285 and 26,241 annotated genes were expressed in the intercaruncular (ICAR) and caruncular (CAR) regions, respectively. In aged cows, 350 genes showed higher and 84 lower expression in the ICAR, while 280 were higher and 156 lower in the CAR (P &lt; 0.01, fold change &gt;20 %). In total, 434 and 436 genes were identified as DEGs in ICAR and CAR respectively, including growth factors, receptors, and orphan G-protein-coupled receptors. Metascape analysis of ICAR DEGs revealed enrichment in pathways related to cell growth, growth factor response, lipid biosynthetic, negative regulation of cell adhesion and differentiation, and embryonic morphogenesis. CAR DEGs were enriched in collagen-associated and epithelial differentiation pathways. Ingenuity Pathway Analysis (IPA) of ICAR DEGs highlighted networks involving oxidative stress-induced senescence, arachidonic acid metabolism, epithelial adherens junction signaling, and tight junction signaling, as well as canonical pathways of G-protein-coupled receptor signaling, and glycosaminoglycan metabolism (P &lt; 0.01). IPA of CAR DEGs indicated enrichment in nonsense mediated decay, collagen chain trimerization, and wound healing signaling pathway. These findings identify DEGs and signaling pathways associated with aging in the bovine endometrium.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"970 ","pages":"Article 149764"},"PeriodicalIF":2.4,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145085747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolution of highly diverse gene orders in mitochondrial genomes of Syllidae (Annelida).","authors":"Gudrun Schwarze, Thilo Schulze, Daisuke S Sato, Toru Miura, María Teresa Aguado","doi":"10.1016/j.gene.2025.149763","DOIUrl":"https://doi.org/10.1016/j.gene.2025.149763","url":null,"abstract":"<p><p>Mitochondrial genomes are extensively utilized in phylogenetic and gene order analyses. While earlier studies suggested a conserved mitochondrial gene order within Pleistoannelida, recent findings indicate notable rearrangements, particularly among lineages in the Syllidae within Errantia. This study investigates 13 newly sequenced mitochondrial genomes (9 complete, 4 partial), uncovering complex gene order variations and offering deeper insights into their evolutionary relationships. Phylogenetic analyses confirm well-supported groups previously identified through single-gene studies, additionally, the genera Brachysyllis and Parasphaerosyllis are included in a molecular phylogenetic analysis for the first time. The phylogenetic relationships of these two genera within Syllidae are finally clarified. A total of eight different patterns other than Pleistoannelida gene order pattern are represented in Syllidae. Within Syllidae, Syllinae is the group where this varies more often and more drastically from the Pleistoannelida ground pattern. When excluding tRNAs, which tend to have higher variability in location, Streptosyllis sp. shows the greatest similarity to the Pleistoannelida ground pattern. In contrast, Syllis okadai and Megasyllis nipponica exhibit the most divergent gene order, followed by Syllis sp. The presented dataset enabled to conduct phylogenetic analyses involving a smaller number of taxa compared to earlier studies but with a substantially larger dataset containing 17 genes and spanning 13,000 to 20,000 base pairs. The findings provide further evidence on the phylogenetic relationships within the family Syllidae. The represented subfamilies are confirmed to be monophyletic and well-supported, and a newly identified, robust clade comprising Amblyosyllis and Brachysyllis is unveiled.</p>","PeriodicalId":12499,"journal":{"name":"Gene","volume":" ","pages":"149763"},"PeriodicalIF":2.4,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145069469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression patterns of PIK3CA and PTEN in cancer and type 2 diabetes mellitus","authors":"Donovan Ariel Rivero ,&nbsp;Jacqueline Lisset Tomsich ,&nbsp;Paula Agustina Benegas ,&nbsp;María Florencia Gallardo ,&nbsp;Elizabeth Méndez ,&nbsp;María Betania Mascheroni ,&nbsp;Marcela Kober ,&nbsp;Pedro Darío Zapata ,&nbsp;Cristian Alberto Ferri","doi":"10.1016/j.gene.2025.149748","DOIUrl":"10.1016/j.gene.2025.149748","url":null,"abstract":"<div><div>Type 2 diabetes mellitus (T2DM) and cancer share key metabolic and molecular pathways, including the PI3K/PTEN/AKT signaling cascade, which plays a central role in glucose metabolism and cell proliferation. This study aimed to evaluate the expression levels of <em>PIK3CA</em> and <em>PTEN</em> genes in individuals with cancer and T2DM, to explore their potential role in cancer progression among diabetic patients. A total of 115 individuals were categorized into four groups: healthy controls (C, n = 27), patients with T2DM (SD, n = 30), cancer patients (Ca, n = 28), and patients with both cancer and diabetes (CD, n = 30). Gene expression was analyzed by real-time PCR, and biochemical parameters were assessed. Statistical analysis included the Kruskal–Wallis and Spearman correlation tests. The expression of the <em>PIK3CA</em> gene was significantly higher in the Ca group compared to the other groups (p = 0.0435), with 77 % of cancer patients exhibiting overexpression. In contrast, only 37 % of CD patients showed <em>PIK3CA</em> overexpression. The expression of the <em>PTEN</em> gene was significantly lower in the SD and CD groups compared to the Ca group (p &lt; 0.05). No significant differences in <em>PIK3CA</em> expression were observed among different cancer types. These findings suggest that <em>PIK3CA</em> and <em>PTEN</em> may serve as potential peripheral indicators of systemic alterations in the PI3K/PTEN pathway in cancer and T2DM, highlighting the need for further research, including tissue validation and biomarker performance assessment.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"969 ","pages":"Article 149748"},"PeriodicalIF":2.4,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145058573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic susceptibility loci and predictive/diagnostic model for gallstone disease risk","authors":"Lili Pan ,&nbsp;Shuang Yao ,&nbsp;Xueting Zhu ,&nbsp;Guanghua Luo","doi":"10.1016/j.gene.2025.149758","DOIUrl":"10.1016/j.gene.2025.149758","url":null,"abstract":"<div><div>This study screened and identified gallstone disease (GSD) high-frequency mutation sites through sequencing of whole blood samples from 197 cases and 191 controls. Subsequently, a self-developed two-dimensional PCR (2D-PCR) method was employed to genotype the GSD susceptibility loci, which were determined through genome-wide association studies (GWAS), in 595 cases and 393 controls: <em>TM4SF4</em> (rs9843304), <em>GCKR</em> (rs1260326), and <em>CYP7A1</em> (rs6471717). We compared the genotype and allele frequencies among these groups, and performed univariate and multivariate logistic regression analyses to identify risk factors for GSD. The R programming language was used to develop a predictive model, validated through receiver operating characteristic (ROC) curves, calibration plots, and decision curve analysis (DCA). After adjusting for sex and age, the rs9843304 TT genotype (OR: 0.51, 95 % CI: 0.36–0.74, <em>P</em> &lt; 0.001) and and rs1260326 TT genotype (OR: 0.65, 95 % CI: 0.45–0.95, <em>P</em> = 0.03) were significantly associated with a lower risk of GSD compared to the CC genotype. Two nomograms were constructed and validated: (1) a comprehensive diagnostic model including liver-function tests, and (2) a streamlined predictive model excluding them. Discrimination, calibration and clinical utility were assessed with ROC curves, calibration plots and DCA. The full model achieved AUCs of 0.800 (training) and 0.806 (validation); the simplified model reached 0.713 and 0.746. Calibration curves indicated a good fit and DCA showed net-benefit thresholds of 0.38–1.00 (training) and 0.30–1.00 (validation) for the simplified model, and 0.18–1.00 for the full model. In summary, 2D-PCR offers an efficient, low-cost platform for GSD genetic screening, and the nomograms enable individualized risk prediction suitable for primary-care and health-check settings.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"969 ","pages":"Article 149758"},"PeriodicalIF":2.4,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145052733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative analysis reveals ferroptosis-related genes with RRM2 as a key driver in endometriosis","authors":"Lanping Zhong ,&nbsp;Yuhong Niu ,&nbsp;Rui Ma ,&nbsp;Yuting Luo ,&nbsp;Xin Li ,&nbsp;Qiru Wang ,&nbsp;Hongxia Xu ,&nbsp;Xiaomin Kang","doi":"10.1016/j.gene.2025.149754","DOIUrl":"10.1016/j.gene.2025.149754","url":null,"abstract":"<div><h3>Background</h3><div>Endometriosis is an estrogen-dependent disorder with unclear molecular mechanisms. Ferroptosis, a regulated iron-dependent cell death, may play a key role, yet the specific genes involved remain underexplored.</div></div><div><h3>Methods</h3><div>Ferroptosis-related genes (FRGs) were identified in endometriosis using GEO data, with key genes selected through LASSO, SVM-RFE, and cross-tabulation analysis. We validated gene expression in the tissues of patients with endometriosis using quantitative real-time polymerase chain reaction (qRT-PCR) and assessed the causal effects of these genes on endometriosis subtypes through Mendelian randomization (MR). We further validated the causal relationship between FRGs with diagnostic value and the progression of endometriosis through in vitro and in vivo experiments.</div></div><div><h3>Results</h3><div>We identified 13 differentially expressed ferroptosis-related genes (DE-FRGs) in endometriosis, including six upregulated and seven downregulated genes. Among these, FZD7, HMOX1, HSPA5, TFAP2C, and RRM2 were identified as key genes with diagnostic potential (AUC =0.91). MR analysis revealed that FZD7, HMOX1, RRM2, and HSPA5 were causally associated with different subtypes of endometriosis, with FZD7 acting as a protective factor (OR &lt; 1), while the others functioned as risk factors (OR &gt; 1). Notably, RRM2 exhibited causal associations across multiple subtypes of the disease. qRT-PCR confirmed significant upregulation of RRM2 in ectopic endometrial tissues (P &lt; 0.01). Functional validation experiments further demonstrated that RRM2 enhances the invasiveness of endometrial cells in vitro (P＜0.05), while in vivo, RRM2 overexpression induced endometriosis-like phenotypes in a mouse model.</div></div><div><h3>Conclusion</h3><div>Our integrative analysis combining transcriptomic profiling, MR, and experimental validation identified FRGs, particularly RRM2, as being causally associated with endometriosis. RRM2 may contribute to disease progression and represents a promising target for diagnosis and therapy.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"970 ","pages":"Article 149754"},"PeriodicalIF":2.4,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145052741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unravelling co-expressed microRNAs in periodontitis − significance for regulatory networks in inflammation and therapeutic targets","authors":"Priyanka Venugopal ,&nbsp;Teena Koshy ,&nbsp;Vamsi Lavu ,&nbsp;Suresh Ranga Rao ,&nbsp;Selvaraj Ramasamy ,&nbsp;Anuradha Dhanasekaran ,&nbsp;Samyuktha Hariharan ,&nbsp;Vettriselvi Venkatesan","doi":"10.1016/j.gene.2025.149759","DOIUrl":"10.1016/j.gene.2025.149759","url":null,"abstract":"<div><h3>Objective</h3><div>This case-control study, elucidates the microRNA profile associated with periodontitis, focusing on deciphering the regulatory mechanisms implicated in the inflammatory phase of periodontitis pathogenesis.</div></div><div><h3>Methods</h3><div>MicroRNA profiling was conducted by Taqman low density array and validation of selected candidates through quantitative PCR. Bioinformatics tools were employed to identify target genes and elucidate their functional roles in periodontitis. Principal component analysis of microRNAs was performed to determine their variability and regulatory patterns in periodontitis pathogenesis.</div></div><div><h3>Results</h3><div><em>miR-146a-5p, miR-125a-5p, miR-20a-5p, miR-155-5p, miR-196a-5p</em>, and <em>miR-499a-5p</em> exhibited significant dysregulation in periodontitis patients, with implications in cytokine signalling, interleukin-1 pathway modulation, and epithelial-mesenchymal transition processes crucial for periodontitis development and progression. Importantly, <em>miR-146a-5p, miR-125a-5p, miR-155-5p, miR-20a-5p</em>, and <em>miR-196a-5p</em> exhibited a pattern of co-expression, indicating a potential functional relationship and thereby provide a pattern to distinguish between individuals with generalized periodontitis and healthy individuals.</div></div><div><h3>Conclusion</h3><div>This study represents a pioneering effort in identifying a microRNA expression pattern which influence disease progression or pathogenesis, and targeting them collectively may offer more effective therapeutic interventions or diagnostic strategies and help to understand microRNA-mediated pathways in periodontal disease management.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"969 ","pages":"Article 149759"},"PeriodicalIF":2.4,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}