Gene最新文献

{"title":"The proximity proteome of pre-40S pre-ribosomal particle components PNO1 and NOB1 using turboID proximity labeling technology","authors":"Xingyuan Xu ,&nbsp;Wenli Chen ,&nbsp;Jiefu Zheng ,&nbsp;Jian-You Liao ,&nbsp;Haiyan Yan ,&nbsp;Shuang Zhu","doi":"10.1016/j.gene.2025.149411","DOIUrl":"10.1016/j.gene.2025.149411","url":null,"abstract":"<div><h3>Background</h3><div>The ribosome assembly factors PNO1 and NOB1 play crucial roles in the maturation of the 40S ribosomal small subunit. TurboID is an efficient biotin ligase that can biotinylate proteins in proximity to the target protein and is widely used to study complex biological processes within cells. In this study, we employed this technology to investigate the complex proximity network of PNO1 and NOB1 within the cell, further revealing their key roles in ribosome biogenesis.</div></div><div><h3>Results</h3><div>Firstly, through immunofluorescence experiments, we observed that PNO1 and NOB1 have different localizations within the cell. Subsequently, by analyzing the proximal proteins labeled by PNO1-TurboID and NOB1-TurboID, we identified 871 proximal proteins for PNO1 and 1044 for NOB1, with 663 overlapping proteins. Functional analysis revealed that these proximal proteins are predominantly enriched in biological processes related to ribosome assembly, rRNA processing, and translation, all of which are closely linked to ribosome biogenesis. Notably, we validated the mass spectrometry-identified proteins through co-IP experiments and found that PNO1 and NOB1 interact with the translation-related proteins EIF4B and EIF4G2.</div></div><div><h3>Conclusion</h3><div>Our study constructed the protein network environment of ribosome assembly factors PNO1 and NOB1 within the cell and found that their neighboring proteins are primarily involved in key biological processes such as ribosome processing, mRNA translation, and the cell cycle, all of which are critical for ribosome biogenesis. These findings provide a valuable foundation for further understanding the roles of PNO1 and NOB1 in ribosome biogenesis and how they regulate this process.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"955 ","pages":"Article 149411"},"PeriodicalIF":2.6,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143737999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FSTL3 promotes colorectal cancer by activating the HIF1 pathway","authors":"Xiang-Rong Luo ,&nbsp;Li-Zhe Huang ,&nbsp;Jie Yin ,&nbsp;Zu-Ming Xiong ,&nbsp;Wen-Xin Li ,&nbsp;Cun Liao ,&nbsp;Ming-Lin Lin ,&nbsp;Wei Huang ,&nbsp;Sen Zhang","doi":"10.1016/j.gene.2025.149435","DOIUrl":"10.1016/j.gene.2025.149435","url":null,"abstract":"<div><div>Follistatin-like 3 (FSTL3) is a glycoprotein known to promote tumor growth, invasion, and angiogenesis in various cancers. However, its role in Colorectal Cancer (CRC), particularly concerning the hypoxia-inducible factor 1α (HIF1α) signaling pathway, remains unclear. The HIF1α pathway is critical in CRC progression, enabling tumor cells to adapt to hypoxia through angiogenesis, Epithelial-Mesenchymal Transition (EMT), and metabolic reprogramming. Analysis of The Cancer Genome Atlas (TCGA) and GSE39582 datasets revealed that FSTL3 is significantly upregulated in CRC tissues and correlates with poor Overall Survival (OS), Progression-Free Survival (PFS), and aggressive features such as venous, lymphatic, and perineural invasion. In vitro experiments demonstrated that FSTL3 overexpression in HCT15 and HCT116 cells promoted proliferation, migration, and cell cycle progression, whereas knockdown in LOVO and Caco2 cells suppressed these processes and induced apoptosis. Transcriptome sequencing and western blot analysis indicated that FSTL3 activated the HIF1α pathway by upregulating HIF1α, ANGPT2, and HK3, which are key regulators of angiogenesis and glycolysis. Importantly, treatment with the HIF1α inhibitor KC7F2 reversed the oncogenic effects of FSTL3 overexpression both in vitro and in vivo. In xenograft and tail vein metastasis models, KC7F2 suppressed tumor growth, reduced pulmonary metastasis, and restored lung tissue integrity, further downregulating FSTL3 and HIF1α expression. These findings suggest that FSTL3 promotes CRC progression via the HIF1α pathway and highlight its potential as a prognostic biomarker and therapeutic target for CRC treatment.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"954 ","pages":"Article 149435"},"PeriodicalIF":2.6,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143725162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pan-cancer analysis identifies EIPR1 as a potential prognostic and immunological biomarker for lung adenocarcinoma and its functional validation","authors":"Xin Zheng ,&nbsp;Xiao Zhang ,&nbsp;Jie Yu ,&nbsp;Jie Zheng","doi":"10.1016/j.gene.2025.149439","DOIUrl":"10.1016/j.gene.2025.149439","url":null,"abstract":"<div><h3>Background</h3><div>EARP and GARP complex-interacting protein 1 (<em>EIPR1</em>) may be a new oncogene in tumors, influencing the prognosis and invasion of cancer. However, a systematic analysis of the function of EIPR1 in various cancers remains vacant. Thus, we proceeded with a comprehensive analysis to ascertain the role of EIPR1 among various cancers.</div></div><div><h3>Methods</h3><div>We explored <em>EIPR1</em> expression in pan-cancer, and its association with clinical stage, survival, gene mutations and methylation by the TIMER 2.0, GEPIA2, cBioPortal, and UALCAN. The protein–protein interaction (PPI) network, immune infiltration, and immune checkpoint assessments of EIPR1 was performed using the STRING and SangerBox. The role of <em>EIPR1</em> expression in lung adenocarcinoma (LUAD) was explored by the R software. The impact of EIPR1 expression on LUAD progression was studied through in vitro assays.</div></div><div><h3>Results</h3><div><em>EIPR1</em> was overexpressed in most cancers and revealed as a potential prognostic biomarker in tumors, involving in tumorigenesis by affecting its methylation and gene mutations. The immune infiltration and immune checkpoints of tumors were related to the expression of <em>EIPR1</em>. Additionally, <em>EIPR1</em> expression affected the survival, diagnosis, clinicopathological features, tumor microenvironment, and drug sensitivity of LUAD patients. Validation studies demonstrated that EIPR1 knockdown suppressed the malignant growth, invasion, and migration of LUAD cells.</div></div><div><h3>Conclusions</h3><div>This study delivers an extensive landscape for the oncogenesis and immunological characteristics of EIPR1, which reveals that EIPR1 may serve as a potential biological target for future prognosis and immune treatment in tumors, especially in LUAD.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"954 ","pages":"Article 149439"},"PeriodicalIF":2.6,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143735238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic and cellular context-dependent expression of the human ELMO1 gene transcript variants","authors":"Nobuyo Maeda ,&nbsp;Lauren S. Taylor ,&nbsp;Melanie Nassar-Guifarro ,&nbsp;Mohamed-Yahia S. Monawar ,&nbsp;Sierra M. Dunn ,&nbsp;Nicholas A. Devanney ,&nbsp;Feng Li ,&nbsp;Lance A. Johnson ,&nbsp;Yukako Kayashima","doi":"10.1016/j.gene.2025.149438","DOIUrl":"10.1016/j.gene.2025.149438","url":null,"abstract":"<div><div>Engulfment and cell motility protein 1 (Elmo1) forms a complex with Dedicator of cytokinesis (Dock) 1–5 and promotes GTP-loading of Rac1, the major agent of cell movement. While the pathophysiological roles of Elmo1 have expanded from apoptotic cell engulfment to cancer, inflammation, diabetic nephropathy and cardiomyopathy, little information is available on its transcriptional regulation. Genome databases indicate at least five transcript variants for human <em>ELMO1</em>: the variants <em>V1</em>, <em>V4</em> and <em>V5</em> encode a full-length 727 aa protein, whereas <em>V2</em> and <em>V3</em> encode a truncated Elmo1 of 247 aa that lacks N-terminal domains. A CpG island promoter drives the major <em>V1</em> transcript, while an <em>LTR12</em> drives <em>V5</em> in intron 1, one of the three <em>LTR12</em> family of retroviral elements in <em>ELMO1</em>. In contrast, the short-forms <em>V2</em> and <em>V3</em> contain CAT-TATA type promoters. Examination of various cell lines by RT-qPCR designed to detect individual transcripts showed that basal transcriptions of the variants were very low to undetectable in cultured cells. However, treatments with Trichostatin A, a histone deacetylase inhibitor, or with 5-Aza-2′-deoxycytidine, a DNA methyl transferase inhibitor, significantly upregulated <em>V1</em>, <em>V4</em>, <em>V5</em> and <em>V2</em> expression in a cell line-specific manner, indicating that these transcripts are epigenetically regulated. Another <em>LTR12D</em> transposon in intron 13 also drives an unannotated transcript stimulated by these inhibitors. Finally, we found the levels of <em>V2</em> transcripts in the mouse and human brain exceed those of <em>V1</em>, suggesting a brain-specific regulation and role of V2 protein.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"954 ","pages":"Article 149438"},"PeriodicalIF":2.6,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic analysis and metabolic pathway mining of Phallus cremeo-ochraceus","authors":"Yiming Nie,&nbsp;Yiguo He,&nbsp;Zhifeng Zhao,&nbsp;Jing Zhang,&nbsp;Xingxiu Zhao","doi":"10.1016/j.gene.2025.149424","DOIUrl":"10.1016/j.gene.2025.149424","url":null,"abstract":"<div><div><em>Phallus cremeo-ochraceus</em> is a nutritious edible mushroom. In this study, after tissue isolation, 33 strains were obtained, among which one strain PC7 with rapid mycelial growth and stable passage was obtained. Here, after quality control and assembly, a sequence of 410,647,36 bp was obtained, with 20,218 contigs. 23,184 genes were predicted, 8,092 were repetitive sequences, and 165 were non-coding RNA (ncRNA), which was obtained by the Illumina platform. NCBI Blast+ was used to compare the protein sequences of the genes with several databases, such as NR, KOG, GO, KEGG, CAzy, etc. The NR database annotated 18,159 genes, KOG annotated 6752 genes, GO annotated 5872 genes, and KEGG annotated 3820 genes. Exploration of the terpenoid synthesis pathway of strain PC7 by KEGG, the results revealed that the genome of PC7 has a more complete metabolic pathway regarding terpenoid synthesis. The analysis of amino acid metabolic pathways shows that <em>Phallus cremeo-ochraceus</em> contains 18 genes related to amino acid metabolic pathways. Combined with carbohydrate enzyme annotation and KEGG annotation, the genes related to carbon source, nitrogen source, sulfur source and growth factor of Phallus cremeo-ochraceus were analyzed, which enriched the related genomic research of Phallus cremeo-ochraceus.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"954 ","pages":"Article 149424"},"PeriodicalIF":2.6,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143729396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated transcriptome and DNA methylome analysis reveal the browning mechanism in Agaricus bisporus","authors":"Guixuan Li ,&nbsp;Depin Feng ,&nbsp;Kebin Li ,&nbsp;Shaopeng Han ,&nbsp;Yang Lv ,&nbsp;Zhuying Deng ,&nbsp;Gongjian Zeng ,&nbsp;Xin’er Qin ,&nbsp;Xiangling Shen ,&nbsp;Shiling Liu","doi":"10.1016/j.gene.2025.149437","DOIUrl":"10.1016/j.gene.2025.149437","url":null,"abstract":"<div><div>The white button mushroom (<em>Agaricus bisporus</em>), widely cultivated worldwide as an edible mushroom, is susceptible to browning, which significantly impacts its nutritional and commercial value. Extensive research has enhanced our understanding of the mechanisms underlying this browning process. Although the role of DNA methylation in regulating gene expression has been studied in many fungi, information specifically concerning DNA methylation during the browning in <em>A. bisporus</em> is still limited. In this study, we initially evaluated the impact of temperatures (4 ℃ and room temperature) on discoloration in <em>A. bisporus</em>, and samples with similar discoloration under different temperatures were collected for transcriptome and DNA methylation sequencing. The results revealed that DNA methylation was positively correlated with browning, suggesting its involvement during the browning in <em>A. bisporus</em>. Further analysis showed the heightened methylation levels were primarily attributed to increased methylation at CHG and CHH sites. By joint analysis of transcriptome and DNA methylome, 342 genes with significant expression changes were identified to be affected by DNA methylation, and finally 13 genes were considered as important browning genes under different signaling pathways, such as ABA/ET pathway. Notably, four DNA methyltransferases were identified and validated to play important role during browning in <em>A. bisporus</em>. Altogether, this study provides theoretical insights into the functions of DNMTs in <em>A. bisporus</em>, and offers new perspectives on the role of DNA methylation in edible mushrooms.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"955 ","pages":"Article 149437"},"PeriodicalIF":2.6,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic gene expression profile of colorectal cancer","authors":"Mohamed J. Saadh ,&nbsp;Omer Qutaiba B. Allela ,&nbsp;Radhwan Abdul Kareem ,&nbsp;Lalji Baldaniya ,&nbsp;Suhas Ballal ,&nbsp;Raghav Vashishth ,&nbsp;Manisha Parmar ,&nbsp;Hayder Naji Sameer ,&nbsp;Atheer Khdyair Hamad ,&nbsp;Zainab H. Athab ,&nbsp;Mohaned Adil","doi":"10.1016/j.gene.2025.149433","DOIUrl":"10.1016/j.gene.2025.149433","url":null,"abstract":"<div><div>Colorectal cancer is a major global health burden, with significant heterogeneity in clinical outcomes among patients. Identifying robust prognostic gene expression signatures can help stratify patients, guide treatment decisions, and improve clinical management. This review provides an overview of current prognostic gene expression profiles in colorectal cancer research. We have synthesized evidence from numerous published studies investigating the association between tumor gene expression patterns and patient survival outcomes. The reviewed literature reveals several promising gene signatures that have demonstrated the ability to predict disease-free survival and overall survival in CRC patients, independent of standard clinicopathological risk factors. These genes are crucial in fundamental biological processes, including cell cycle control, epithelial-mesenchymal transition, and immune regulation. The implementation of prognostic gene expression tests in clinical practice holds great potential for enabling more personalized management strategies for colorectal cancer.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"955 ","pages":"Article 149433"},"PeriodicalIF":2.6,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Population genetic structure of the fibrinogen-related protein 1 (FREP1) in Iranian isolates of Anopheles stephensi as a promising mosquito-based malaria vaccine candidate","authors":"Omolbani Kheirkhah,&nbsp;Sakineh Pirahmadi,&nbsp;Akram Abouie Mehrizi,&nbsp;Abbasali Raz","doi":"10.1016/j.gene.2025.149436","DOIUrl":"10.1016/j.gene.2025.149436","url":null,"abstract":"<div><h3>Background</h3><div>Transmission Blocking Vaccines (TBVs) are critical for malaria eradication as they aim to interrupt the transmission cycle of <em>Plasmodium</em> parasites. However, the development of effective TBVs is challenged by the antigenic diversity of potential vaccine targets. This study investigates the fibrinogen-related protein 1 (<em>frep1</em>) gene, a promising candidate for TBVs, focusing on the genetic diversity of the <em>frep1</em> gene and immunogenic potential in <em>Anopheles stephensi</em> from southern Iran.</div></div><div><h3>Methods</h3><div>Analysis of 30 <em>An. stephensi</em> isolates collected from three malaria-endemic cities was carried out using molecular amplification, sequencing, and bioinformatics tools. The haplotype and nucleotide diversity were assessed using the DnaSP, while predicted linear B-cell epitopes were evaluated through the ABCpred. Additionally, conformational epitopes were identified via DiscoTope analysis and visualized using the PyMOL. Furthermore, MHC II-peptide interactions were examined to evaluate the potential for effective immune responses.</div></div><div><h3>Results</h3><div>Seven distinct haplotypes of the <em>frep1</em> domain were identified, with synonymous nucleotide variations indicating high genetic conservation. This conservation was complemented by the identification of 16 linear B-cell epitopes with high immunogenic scores (0.80–0.97). Conformational epitopes, prominently located at the N-terminus and C-terminus, were consistent across isolates. MHC II-binding analysis revealed peptides with strong affinities to prevalent HLA alleles in Iran, suggesting effective antigen presentation.</div></div><div><h3>Conclusion</h3><div>FREP1 demonstrates purifying selection, low genetic diversity and high immunogenic potential, highlighting the suitability of the protein as a TBV candidate. The presence of conserved epitopes and strong MHC II interactions supports the potential of this protein for inducing robust immune responses. However, regional haplotype diversity suggests that vaccine strategies may need to account for local variability. These findings underscore the promise of FREP1 for TBV development and emphasize the need for further validation in vivo.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"953 ","pages":"Article 149436"},"PeriodicalIF":2.6,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human-genetic variants associated with susceptibility to SARS-CoV-2 infection","authors":"Daniel Azcarate ,&nbsp;Felix Olasagasti Arsuaga ,&nbsp;Eva Granizo Rodriguez ,&nbsp;Eunate Arana-Arri ,&nbsp;Pedro Pablo España ,&nbsp;Maider Intxausti ,&nbsp;Cristina Sancho ,&nbsp;Aitor García de Vicuña Meléndez ,&nbsp;Oliver Ibarrondo ,&nbsp;Marian M. de Pancorbo","doi":"10.1016/j.gene.2025.149423","DOIUrl":"10.1016/j.gene.2025.149423","url":null,"abstract":"<div><div>SARS-CoV-2, the third major coronavirus of the 21st century, causing COVID-19 disease, profoundly impacts public health and workforces worldwide. Identifying individuals at heightened risk of SARS-CoV-2 infection is crucial for targeted interventions and preparedness. This study investigated 35 SNVs within viral infection-associated genes in SARS-CoV-2 patients and uninfected controls from the Basque Country (March 2020–July 2021). Its primary aim was to uncover genetic markers indicative of SARS-CoV-2 susceptibility and explore genetic predispositions to infection. Association analyses revealed previously unreported associations between SNVs and susceptibility. Haplotype analyses uncovered novel links between haplotypes and susceptibility, surpassing individual SNV associations. Descriptive modelling identified key susceptibility factors, with rs11246068-CC (IFITM3), rs5742933-GG (ORMDL1), rs35337543-CG (IFIH1), and GGGCT (rs2070788, rs2298659, rs17854725, rs12329760, rs3787950) variation in TMPRSS2 emerging as main infection-susceptibility indicators for a COVID-19 pandemic situation. These findings underscore the importance of integrated SNV and haplotype analyses in delineating susceptibility to SARS-CoV-2 and informing proactive prevention strategies. The genetic markers profiled in this study offer valuable insights for future pandemic preparedness.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"953 ","pages":"Article 149423"},"PeriodicalIF":2.6,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-660: A novel regulator in human cancer pathogenesis and therapeutic implications","authors":"Zehua Wang ,&nbsp;Xinming Su ,&nbsp;Zhiqing Zhan ,&nbsp;Hangxuan Wang,&nbsp;Shuhan Zhou,&nbsp;Jiasheng Mao,&nbsp;Hening Xu,&nbsp;Shiwei Duan","doi":"10.1016/j.gene.2025.149434","DOIUrl":"10.1016/j.gene.2025.149434","url":null,"abstract":"<div><div>MicroRNAs (miRNAs) are non-coding RNAs that regulate gene expression. Among these, miR-660, located on chromosome Xp11.23, is increasingly studied for its role in cancer due to its abnormal expression in various biological contexts. It is regulated by 8 competing endogenous RNAs (ceRNAs), which adds complexity to its function. miR- 660 targets 19 genes involved in 6 pathways such as PI3K/AKT/mTOR, STAT3, Wnt/β-catenin, p53, NF‑κB, and RAS, influencing cell cycle, proliferation, apoptosis, and invasion/migration. It also plays a role in resistance to chemotherapies like cisplatin, gemcitabine, and sorafenib in lung adenocarcinoma (LUAD), pancreatic ductal adenocarcinoma (PDAC), and hepatocellular carcinoma (HCC), thus highlighting its clinical importance. Additionally, leveraging liposomes as nanocarriers presents a promising avenue for enhancing cancer drug delivery. Our comprehensive study not only elucidates the aberrant expression patterns, biological functions, and regulatory networks of miR-660 and its ceRNAs but also delves into the intricate signaling pathways implicated. We envisage that our findings will furnish a robust framework and serve as a seminal reference for future investigations of miR-660, fostering advancements in cancer research and potentially catalyzing breakthroughs in cancer diagnosis and treatment paradigms.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"953 ","pages":"Article 149434"},"PeriodicalIF":2.6,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}