Gene最新文献

{"title":"Gene Expression Profiling of Maslinic Acid-treated MCF-7 Breast Cancer Cells Using Nanostring nCounter Pancancer Pathway Panel","authors":"Soon Yan Tan ,&nbsp;Chai Nien Foo ,&nbsp;Foong Leng Ng ,&nbsp;Chee Hong Tan ,&nbsp;Yang Mooi Lim","doi":"10.1016/j.gene.2024.149043","DOIUrl":"10.1016/j.gene.2024.149043","url":null,"abstract":"<div><div>Breast cancer remains a significant global health concern, impacting millions of women every year<strong>.</strong> Maslinic acid (MA), a pentacyclic triterpene has been found to exert promising anticancer effect in various cancers, including breast cancer, yet the underlying mechanisms remain unclear. This study aims to elucidate the anticancer properties of MA via gene expression profiles in breast cancer cells. Cytotoxicity assay results revealed that MCF-7 exerts the highest sensitivity after 72 h of MA treatment followed by T-47D and MDA-MB-231. MCF-7 were then selected for in-depth analysis using the Nanostring nCounter Pancancer Pathway Panel to analyze the differential expression of genes (DEGs). Across three time points (24, 48, and 72 h), 20 significant DEGs were identified, of which 5 were upregulated and 15 were downregulated. In silico analysis indicated that these DEGs were involved in Pathway of Cancer, Focal Adhesion-PI3K-mTOR Signaling Pathway, PI3K-Akt, and Ras Signaling Pathway. The regulation of these DEGs contributes to several cellular activities such as apoptosis, inhibition of cell proliferation, cell cycle and survival, reduction of glycolysis, angiogenesis, and DNA repair. Additionally, the unfolded protein response emerged as a noteworthy biological process in this study. This study unravels the molecular mechanisms underpinning the therapeutic potential of MA against breast cancer.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"935 ","pages":"Article 149043"},"PeriodicalIF":2.6,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142564239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variants that get straight to your heart – Cardiogenetic secondary findings in exome sequencing","authors":"Kirsten Wenderholm ,&nbsp;Theresa Brunet ,&nbsp;Elisabeth Graf ,&nbsp;Marie Arens ,&nbsp;Eimo Martens ,&nbsp;Juliane Winkelmann ,&nbsp;Julia Hoefele ,&nbsp;Dominik S. Westphal","doi":"10.1016/j.gene.2024.149063","DOIUrl":"10.1016/j.gene.2024.149063","url":null,"abstract":"<div><h3>Background</h3><div>Exome sequencing has been established as a fundamental tool in genetic diagnostics. It may also provide information about variants in genes unrelated to the primary purpose, so-called secondary findings. Especially, diagnoses of unnoticed inborn cardiac diseases are of high clinical relevance due to therapeutic options in context of prevention of sudden cardiac death.</div></div><div><h3>Methods</h3><div>Exome data of 9962 individuals was analysed for relevant cardiogenetic findings. Genes were selected according to ACMG recommendations for secondary findings (v.3.1). First, a filter for (likely) pathogenic variants, published in the ClinVar database, was used. Second, exome data was screened for loss of function (LoF) variants in genes in which LoF is a known disease pathomechanism. All variants were evaluated by geneticists regarding their pathogenicity.</div></div><div><h3>Results</h3><div>Pathogenic or likely pathogenic variants were identified in 136 different individuals (136/9962, 1.4%), with the Low-Density Lipoprotein Receptor gene (<em>LDLR</em>, 24/136, 17.6%) and the Titin gene (<em>TTN</em>, 24/136, 17.6%), being the most frequently affected ones. 31.6% (43/136) of the identified variants had been reported beforehand, while 47.1% (64/136) had not been reported. The remaining cases (29/136, 21.3%) were part of research projects with no written reports. In 26.5% (36/136), the finding would have been missed, if only index patients and not their parents had been screened for secondary findings in case of trio ES.</div></div><div><h3>Conclusion</h3><div>As demonstrated in our study, at least one or two out of one hundred people are likely to carry a pathogenic cardiogenetic variant. Counselling geneticist and clinicians need to be aware of these findings in exome and genome sequencing. Informed consent of the patient regarding the report of secondary findings should absolutely be obtained beforehand.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"935 ","pages":"Article 149063"},"PeriodicalIF":2.6,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142564245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial genome of Neuryurus rudis (Xenarthra, Cingulata); contribution to phylogeny and origin of glyptodonts","authors":"Luciano Brambilla ,&nbsp;Damián A. Ibarra ,&nbsp;María C. Barboza ,&nbsp;Edgardo G. Bresso ,&nbsp;Germán Rosano ,&nbsp;Germán Pérez ,&nbsp;Pablo Straccia ,&nbsp;Rubén D. Scian ,&nbsp;Lucas R. Brun","doi":"10.1016/j.gene.2024.149059","DOIUrl":"10.1016/j.gene.2024.149059","url":null,"abstract":"<div><div>The remarkable glyptodonts have sparked the interest of evolutionary biologists since the 19th century, in their attempts to elucidate the phylogenetic relationships among the various species of these armored giants and their relationship with other xenarthrans. In recent years, the molecular analysis of the first glyptodont has included them within the cingulates, as a special group of armadillos that lost the mobility of the bands of their armor during their evolutionary history. In this research, we obtained the mitochondrial DNA sequence of the elusive and poorly known glyptodont <em>Neuryurus rudis</em>, inferring its phylogenetic position with respect to the glyptodont <em>Doedicurus</em> sp. and extant armadillos. This study reaffirms glyptodonts as a subgroup of cingulates, with <em>Neuryurus</em> and <em>Doedicurus</em> sharing a common ancestor from the late Oligocene or early Miocene and traces the group’s origin back to an armadillo ancestor in the Eocene.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"936 ","pages":"Article 149059"},"PeriodicalIF":2.6,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gardner syndrome in a Tunisian family: Identification of a rare APC mutation through targeted NGS","authors":"Rania Abdelmaksoud-Dammak ,&nbsp;Nihel Ammous-Boukhris ,&nbsp;Souhir Guidara ,&nbsp;Hassen Kamoun ,&nbsp;Hela Gdoura ,&nbsp;Baha Barkia ,&nbsp;Mouna Boudabbous ,&nbsp;Nabil Tahri ,&nbsp;Hazem Ben Ameur ,&nbsp;Salah Boujelbene ,&nbsp;Raja Mokdad Gargouri","doi":"10.1016/j.gene.2024.149065","DOIUrl":"10.1016/j.gene.2024.149065","url":null,"abstract":"<div><div>Gardner syndrome (GS) is a subtype of familial adenomatous polyposis (FAP) characterized by colorectal polyps, multiple osteomas, soft tissue tumors, and specific oral manifestations, such as jaw osteomas. GS is caused by mutations in the <em>APC</em> gene, resulting in a nonfunctional protein. This study reports a comprehensive clinical evaluation and genetic analysis of a Tunisian family affected by GS. Targeted exome sequencing and Sanger sequencing techniques were employed to identify and validate mutations in the <em>APC</em> gene. Clinical observations of the patient revealed multiple sebaceous cysts, frontal and maxillary osteomas, and several gastrointestinal polyps. Genetic analysis revealed a pathogenic variant (c.4652-4655del) in the <em>APC</em> gene, leading to a truncated protein. Additionally, genetic testing of the patient’s child indicated that the child does not carry the APC pathogenic variant.</div><div>In conclusion, our study highlights the importance of genetic testing in raising awareness of GS among clinicians to ensure early diagnosis and effective management, thereby reducing the risk of development and progression of colorectal cancer.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"935 ","pages":"Article 149065"},"PeriodicalIF":2.6,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142564208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The pertinence of resistin gene single nucleotide polymorphism G > A and its expression in oral cancer","authors":"Kiran Arif ,&nbsp;Fouzia Shaikh ,&nbsp;Rizma Khan ,&nbsp;Faraz Ahmed Baig ,&nbsp;Talat Mirza","doi":"10.1016/j.gene.2024.149062","DOIUrl":"10.1016/j.gene.2024.149062","url":null,"abstract":"<div><h3>Background/aim</h3><div>Oral cancer (OC) is the leading cause of fatalities in Pakistan among males due to inadequate oral hygiene and chewing habits. However, genetic susceptibility patterns also play a critical role in disease progression. Since the frequency of <em>Resistin</em> (<em>RETN)</em> SNP (Single nucleotide polymorphism) <em>rs3219175</em> is unknown; there is a requirement for early diagnosis of the OC. Therefore, the current study aims to determine the frequency of targeted SNP and develop a safe, simple, and fast alternative technique for better treatment using a real-time PCR assay with HRM (high-resolution melting curve) analysis.</div></div><div><h3>Materials and methods</h3><div>A case-control study was conducted on 35 Oral squamous cell carcinomas (OSCC) diagnosed patients and 35 healthy individuals. HRM and RT-PCR results were analysed by the bioinformatics analyses.</div></div><div><h3>Results</h3><div>The frequency of <em>RETN</em> SNP <em>rs3219175</em> genotypes GG and GA in male patients was 16 (46 %) and 5 (14 %) respectively and in females 8 (23 %) and 6 (17 %) respectively. The chi-square test of independence consummated the assessment between males and females in both control and patients. The relation between these variables was significant (p &lt; 0.05). The interaction network of String 8.3 demonstrates strong interactions at a high confidence score, which helps to characterize functional disorders that may be a causative factor for oral pathology. Reactome and KEGG data were acquired to rule out the pathway involvement of the targeted gene. MuPIT software was used to identify the 3D structure or RETN and their expected mutation effect.</div></div><div><h3>Conclusion</h3><div>This study provides baseline data regarding the frequency of <em>RETN</em> SNP <em>rs3219175</em> among the Pakistani population. For further clarification of their stage in cancer emergence and growth, large-scale studies must be conducted. This study might be helpful in the precision medicine approach and provide better therapeutic for OSCC patients.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"935 ","pages":"Article 149062"},"PeriodicalIF":2.6,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of cathepsins on liver hepatocellular carcinoma: Insights from genetic and functional analyses","authors":"Qi Liu ,&nbsp;Junyi Chen ,&nbsp;Yuyang Liu ,&nbsp;Shengwei Zhang ,&nbsp;Hui Feng ,&nbsp;Tao Wan ,&nbsp;Shemin Zhang ,&nbsp;Ning Zhang ,&nbsp;Zhanyu Yang","doi":"10.1016/j.gene.2024.149064","DOIUrl":"10.1016/j.gene.2024.149064","url":null,"abstract":"<div><div>Liver Hepatocellular Carcinoma (LIHC), ranked as the second deadliest cancer globally, poses a major health challenge because of its widespread occurrence and poor prognosis. The mechanisms underlying LIHC development and progression remain unclear. Cathepsins are linked to tumorigenesis in other cancers, but their role in LIHC is underexplored. This study employed integrative analyses, including Mendelian Randomization (MR), bulk RNA-sequencing (bulk-seq), single-cell RNA sequencing (scRNA-seq), immunohistochemical (IHC) analysis, and cellular experiments with siRNA technology, to investigate the role of cathepsin E (CTSE) in LIHC. MR analysis identified CTSE as a factor associated with increased LIHC risk. Prognostic analysis using TCGA data showed that higher CTSE levels are linked to poorer survival, establishing CTSE as an independent prognostic risk factor. Integrative transcriptome analysis revealed close relation of CTSE to the extracellular matrix. scRNA-seq from TISCH2 demonstrated that CTSE is predominantly expressed in malignant LIHC cells. IHC confirmed higher CTSE expression in LIHC tissues compared to peritumoral tissues. Functional assays, such as qRT-PCR, Western blot, cell proliferation, and colony formation experiments, demonstrated that siRNA-mediated CTSE knockdown in HepG2 and Huh7 cell lines notably suppressed cell proliferation and altered the FAK/Paxillin/Akt signaling cascade. This research enhances our comprehension of LIHC development, emphasizing CTSE as a promising prognostic marker and potential therapeutic target. Inhibiting CTSE could slow the progression of LIHC, presenting novel opportunities for therapeutic approaches.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"935 ","pages":"Article 149064"},"PeriodicalIF":2.6,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142564216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CircRNA regulates lung cancer metastasis","authors":"Han Li ,&nbsp;Fan wu ,&nbsp;Yaqi Han ,&nbsp;Ziyi Guo ,&nbsp;Tangbing Chen ,&nbsp;Zhongliang Ma","doi":"10.1016/j.gene.2024.149060","DOIUrl":"10.1016/j.gene.2024.149060","url":null,"abstract":"<div><div>Lung cancer stands prominently among the foremost contributors to human mortality, distinguished by its elevated fatality rate and the second-highest incidence rate among malignancies. The metastatic dissemination of lung cancer stands as a primary determinant of its elevated mortality and recurrence rates, underscoring the imperative for comprehensive investigation into its metastatic pathways. Circular RNAs (circRNAs), a subclass of non-coding RNA (ncRNA) molecules, have garnered attention for their pivotal involvement in the genesis and advancement of lung cancer. Emerging evidence highlights the indispensable functions of circRNAs in orchestrating the metastatic cascade of lung cancer. This review primarily discusses the mechanisms by which circRNAs act as competitive endogenous RNAs (ceRNAs) and modulate various signaling pathways to regulate lung cancer metastasis. CircRNAs influence critical cellular processes including angiogenesis, autophagy, and glycolysis, thereby exerting influence over the metastatic cascade in lung cancer. These discoveries offer innovative perspectives and therapeutic avenues for the diagnosis and management of lung cancer.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"935 ","pages":"Article 149060"},"PeriodicalIF":2.6,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tanshinone T1/T2A inhibits non-small cell lung cancer through Lin28B-let-7-BORA/MYC regulatory network","authors":"Yanli Li ,&nbsp;Ziyi Guo ,&nbsp;Ping Li ,&nbsp;Jing Guo ,&nbsp;Huimin Wang ,&nbsp;Wei Pan ,&nbsp;Fan Wu ,&nbsp;Jingjing Li ,&nbsp;Jinrong Zhou ,&nbsp;Zhongliang Ma","doi":"10.1016/j.gene.2024.149058","DOIUrl":"10.1016/j.gene.2024.149058","url":null,"abstract":"<div><h3>Background</h3><div>Lung cancer is the leading cause of cancer-related deaths worldwide. Tanshinones are a group of compounds in <em>Salvia miltiorrhiza</em>. Although the effects of tanshinone I (T1) and tanshinone IIA (T2A) are widely concerned, the mechanisms of T1 and T2A in lung cancer is rarely studied.</div></div><div><h3>Experimental procedure</h3><div>Xenograft tumor growth was performed to detect the role of T1/T2A in vivo. Next-generation sequencing of miRNA expression profiles in T1/T2A-treated A549 cells showed that T1/T2A upregulated the expression of the let-7 family. Then, let-7a-5p and its downstream target gene <em>BORA</em> were identified as the research objects in this paper. Mechanistically, we examined the interplay between miR-let-7 and <em>BORA</em> through the dual-luciferase reporter assay. Finally, the potential regulatory role of T1/T2A on <em>Lin28B</em> and <em>MYC</em> was explored.</div></div><div><h3>Results</h3><div>This study found that the let-7 family was significantly up-regulated via “Next-generation” sequencing (NGS) in the T1/T2A-treated A549 cell line, while <em>BORA</em> was downregulated. <em>BORA</em> was confirmed as a direct target of let-7. LncRNA MYCLo-5 was up-regulated after treatment with tanshinones. Knockdown of MYCLo-5 promoted the cell cycle and proliferation of non-small cell lung cancer (NSCLC) cells.</div></div><div><h3>Conclusions</h3><div>This study explored the effects of tanshinone T1 and T2A on NSCLC <em>in vitro</em> and <em>in vivo</em>, revealing the T1/T2A-let-7/<em>BORA</em>/MYCLo-5 regulatory pathway, which provided new insights for lung cancer treatment.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"935 ","pages":"Article 149058"},"PeriodicalIF":2.6,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BZW2 is a potential regulator of non-small cell lung cancer progression","authors":"Yan Mo ,&nbsp;Xueyong Feng ,&nbsp;Jincheng Su,&nbsp;Guoyong Chen,&nbsp;Lei Xian","doi":"10.1016/j.gene.2024.149055","DOIUrl":"10.1016/j.gene.2024.149055","url":null,"abstract":"<div><h3>Background</h3><div>Personalized targeted therapy has become an important strategy for cancer treatment owing to its remarkable therapeutic efficacy and safety. However, drug resistance remains the primary cause of treatment failure. Basic leucine zipper and W2 domain 2 (BZW2), which is aberrantly expressed in cancer, has been implicated in tumor progression and may serve as a new therapeutic target. Therefore, the role of BZW2 in non-small cell lung cancer (NSCLC) requires further investigation.</div></div><div><h3>Methods</h3><div>The expression and genetic alterations of BZW2 in pan-cancers were explored using The Cancer Genome Atlas (TCGA) PanCancer databases. The mRNA and protein levels of BZW2 in patients with NSCLC were verified in our cohort. Functional experiments including CCK8, colony formation, and transwell assays were performed to evaluate the impact of BZW2 on the proliferative, migratory, and invasive capacities of SK-MES-1 cells. Gene Set Enrichment Analysis was used to identify underlying biological processes and pathways. Single-cell RNA (scRNA) sequencing data were employed to investigate the tumor microenvironment of NSCLC and the co-expression of BZW2 and stemness-related genes.</div></div><div><h3>Results</h3><div>Dysregulated BZW2 expression was observed in various malignant tumors. BZW2 expression was found to be significantly elevated in NSCLC. BZW2 depletion inhibited the growth, mobility, and invasive abilities of lung squamous cell carcinoma SK-MES-1 cells. BZW2 may be related to signaling pathways such as nucleotide excision repair, ubiquitin-mediated proteolysis, and the P53 signaling pathway. Biological processes, including translational initiation, tRNA processing, and RNA methylation, were observed to be enriched in the high-BZW2 group. Furthermore, there was a positive correlation between BZW2 and the m6A- and m5C-related genes. scRNA analysis revealed a co-expression relationship between BZW2 and stemness-related genes such as CD44, SOX9, and CD133.</div></div><div><h3>Conclusions</h3><div>Elevated BZW2 expression is associated with the proliferation, migration, and invasion of NSCLC, and BZW2 may be a potential therapeutic target for NSCLC.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"935 ","pages":"Article 149055"},"PeriodicalIF":2.6,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide analysis of the MADS-box gene family in mango and ectopic expression of MiMADS77 in Arabidopsis results in early flowering","authors":"Haixia Yu ,&nbsp;Liming Xia ,&nbsp;Jiawei Zhu ,&nbsp;Xiaojie Xie ,&nbsp;Ying Wei ,&nbsp;Xi Li ,&nbsp;Xinhua He ,&nbsp;Cong Luo","doi":"10.1016/j.gene.2024.149054","DOIUrl":"10.1016/j.gene.2024.149054","url":null,"abstract":"<div><div>Mango (<em>Mangifera indica</em> L.) is an important tropical fruit, and timely flowering and fruit setting are very important for mango production. The <em>MADS-box</em> gene family is involved in the regulation of flower induction, floral organ specification, and fruit development in plants. The identification and analysis of the <em>MADS-box</em> gene family can lay a foundation for the study of the molecular mechanism of flowering and fruit development in mango. In this study, 119 <em>MiMADS-box</em> genes were identified on the basis of genome and transcriptome data. Phylogenetic analysis revealed that these genes can be divided into two classes. Forty-one type I proteins were further divided into three subfamilies, and seventy-eight type II proteins were further classified into eleven subfamilies. Several pairs of alternative splicing genes were found, especially in the <em>SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1</em> (<em>SOC1</em>) subfamily. The <em>MiMADS-box</em> genes were distributed on 18 out of the 20 mango chromosomes. Cis-element analysis revealed many light-, stress-, and hormone-responsive elements in the promoter regions of the mango <em>MiMADS-box</em> genes. Expression pattern analysis revealed that these genes were differentially expressed in multiple tissues in mango. The highly expressed <em>MiMADS77</em> was subsequently transformed into Arabidopsis, resulting in significant early flowering and abnormal floral organs. Yeast two-hybrid (Y2H) assays revealed that MiMADS77 interacts with several MiMADS-box proteins. In addition, we constructed a preliminary flowering regulatory network of <em>MADS-box</em> genes in mango on the basis of related studies. These results suggest that <em>MiMADS77</em> genes may be involved in flowering regulation of mango.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"935 ","pages":"Article 149054"},"PeriodicalIF":2.6,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}